Neelesh Sharma

Sher-e-Kashmir University of Agricultural Sciences and Technology Jammu | SKUAST · Veterinary Medicine

The "stem cells" are commonly defined as "cells capable of self-renewal through replication and differentiating into specific lineages". The mammary gland contains functional stem/progenitor cells. The current study was planned with the objectives to study the differentiation dynamics of Korean Holstein mammary epithelial stem cells (KHMESCs) under...

The dairy industry is a multi-billion dollar industry catering the nutritional needs of all age groups globally through the supply of milk. Clinical mastitis has a severe impact on udder tissue and is also an animal welfare issue. Moreover, it significantly reduces animal value and milk production. Mammary tissue damage reduces the number and activ...

The study was conducted on 20 Holstein X Sahiwal cross bred dairy cows, with an average milk production of 2,752±113.79 liters in 284±5.75 days during a single lactation, that were divided in to two groups of 10 animals. We investigated the oxidative stress and antioxidant status during the transition period in dairy cows. In this study, plasma lev...

With the ever-increasing global population, it is impossible to meet the demand for animal protein by relying only on conventional methods due to the depleting natural resources. It is very challenging to ensure a sustainable supply of animal proteins from a single source or form and requires a holistic approach by using all suitable options. The p...

Mastitis is a multi-etiological complex disease of dairy cows and negatively affects the quality and quantity of milk. Milk is a nutritious food for human being; milk quality is negatively affected by intramammary infection of dairy cows. A total of 300 milk samples were collected from mastitis dairy cows irrespective of parity and stage of lactati...

Mastitis is the inflammation of the secretory or alveolar part of the udder and is the most expensive disease of the dairy industry. The estimated economic loss in the US dairy industry alone is approximately $2 billion annually. The substantial increase in economic losses, owing to the high occurrence rate and low recovery rate, is alarming for th...

Abstract The in-vitro meat is a novel concept in food biotechnology comprising field of tissue engineering and cellular agriculture. It involves production of edible biomass by in-vitro culture of stem cells harvested from the muscle of live animals by self-organizing or scaffolding methodology. It is considered as efficient, environmental friendl...

Fast and precise diagnosis of infectious and non-infectious animal diseases and their targeted treatments are of utmost importance for their clinical management. The existing biochemical, serological and molecular methods of disease diagnosis need improvement in their specificity, sensitivity and cost and, are generally not amenable for being used...

Polyvinyl alcohol (PVA) and polyvinyl pyrrolidone (PVP) are the two most investigated biopolymers for various tissue engineering applications. However, their poor tensile strength renders them unsuitable for cardiac tissue engineering (CTE). In this study, we developed and evaluated PVA‐PVP‐based patches, plasticized with glycerol or propylene glyc...

Background Myogenic regulatory factors (MRFs) such as MyoD, Myf6 and Myf5 play vital role in growth and development of muscles. Jeju native pig (JNP) is the top ranker in Korea amongst the indigenous livestock reared for meat purpose. Meager studies covering transcript abundance of the MRFs and related to their co-expression with Pax7 in JNP have b...

Present study was conducted in 240 day-old broiler chicks to assess the effect of condensed tannins (CT) containing guava leaf meal (GLM) supplemented diet on nutrient composition, litter metabolites viz. pH, moisture and ammonia concentration) and microbial counts after completion of feeding trial of 42 days. Chicks were randomly allocated into 4...

Ready-to-use, biodegradable and biocompatible polymeric patch based on the blend of poly(vinyl alcohol) (PVA) and poly(vinyl pyrrolidone) (PVP) polymers and methods for their preparation and use in wound dressing and tissue engineering are disclosed. Applications of such biocompatible patch include closure of clinical wounds, post-operative incisio...

Context. Mastitis is an inflammation of mammary gland parenchyma, and is an unending cause of economic loss to the dairy industry. The interest in research on biomarker discovery for the diagnosis of bovine mastitis stems largely from the need to identify reliable biomarkers. Aim. To determine the putative biomarkers of mastitis by using bioinforma...

Mastitis is the most devastating economic disease of dairy cattle. Mastitis in dairy cattle frequently occurs during the dry period or in early lactation. E. coli. and S. aureus is the main causative agent of mastitis. S. aureus has an ability to form encapsulated focuses in the udder and develop subclinical form of mastitis. This property of bacte...

Early diagnosis and treatment of any disease of animals is important for best results in the veterinary clinics, hence present paper is formulated with the emphasis on updated early diagnostic markers in veterinary science. Besides this cytokines are being used as immunomodulators, biomarkers and may further be used for different diagnostic purpose...

Heart failure is an important cause of morbidity and mortality in canines. W ith the improvement in diagnostic technology, diagnosis of heart failure has become a routine in referral clinics. Sixteen dogs diagnosed with congestive left heart failure, employing history, clinical signs, radiography and electrocardiography were studied for level of ca...

Sus scrofa provides a major source of animal protein for humans as well as being an excellent biomedical model. This study was carried out to understand, in detail, the genetic and functional variants of Jeju Native Pigs and miniature pigs through differential expression profiling of the genes controlling their immune response, growth performance,...

BRM270 is the most leading phytochemical extract that possesses potent anticancer properties. A major challenge associated with this drug is its low bioavailability and thus requires high dosages for cancer treatment. Here, we report the novel nano-synthesis of phyto-composite, BRM270 for the first time by mechanical milling method with specific mo...

Aminoglycoside antibiotics have long been used in antibacterial therapy. Despite their beneficial effects, aminoglycosides have considerable nephrotoxic and ototoxic side effects. So, present study was done to evaluate biochemical and oxidative changes in amikacin induced nephrotoxicity in experimental rats. A total of 24 rats were equally divided...

To know the status of trace minerals in buffaloes from various agro-climatic zones of Jammu division, a baseline survey was conducted. A total of 193 buffalo blood and 169 fodder samples offered during various seasons were collected and analyzed. The average values of Cu, Zn, Fe, Mn and Co in buffalo plasma samples were 14.86, 31.27, 56.05, 1.98 an...

For more than a quarter of a century, sequencing technologies from Sanger’s method to next-generation high-throughput techniques have provided fascinating opportunities in the life sciences. The continuing upward trajectory of sequencing technologies will improve livestock research and expedite the development of various new genomic and technologic...

This study assessed the effect of supplementation of novel transgenic phytase on growth performance and bone mineralization in Korean native broiler chickens. The experiment was designed using four dietary groups: those with a diet supplemented with (A) recombinant phytase, (B) transgenic phytase from the plant Lemna minor, (C) or wild-type L. mino...

The current study was carried out with an aim to determine the prevalence of clinical and subclinical mastitis in dairy buffaloes in both organized and unorganized farms of Jammu and RS Pura region. Screening was done on 150 and 500 randomly selected animals from organized and unorganized farms respectively. Today it can be estimated that nearly ha...

Cytotherapeutics refers to prevention, treatment, cure or mitigation of disease or injuries by the administration of autologous, allogenic or xenogenic cells (manipulated or altered ex vivo). Cells with the power of unlimited self-renewal which when triggered can differentiate into advantageous specialized cell types, such types of cells are known...

The antibacterial and anti-inflammatory properties of lactoferricin have been ascribed to its ability to sequester essential iron. The objective of the study was to clone bovine lactoferricin (LFcinB) gene into PiggyBac Transposon vector, expression study in the bovine mammary epithelial stem cells (bMESCs) and also to determine the antimicrobial p...

Although there are several ways such as karyotyping to evaluate the quality and normality of embryos, the counting of total cell in blastocyst after the differential staining has been used as a simple indicator for quality of culture systems and normality of embryo itself. This differential staining method was regarded as a basic technique of early...

Background Several efforts have been deployed to cure osteosarcoma, a high-grade malignant bone tumour in children and adolescents. However, some challenges such as drug resistance, relapse, and tumour metastasis remain owing to the existence of cancer stem cells (CSC). There is an urgent need to develop cost-effective and safe therapies. Methods...

Hepatocellular carcinoma (HCC) is a major threat to human health worldwide and development of novel antineoplastic drug is demanding task. BRM270 is a proprietary combination of traditional medicinal herbs, has been shown to be effective against a wide range of stem-like cancer initiating cells (SLCICs). However, the underlying mechanism and antitu...

Abstract Recent years have seen an unpretending increase in research using dietary phytochemicals for targeting cancer and cancer stem cells (CSCs) due to the limited efficacy of conventional chemotherapy and radiotherapy and numerous associated side effects. A large number of dietary phytochemicals using traditional recommendation and experimenta...

Wogonin is a plant flavonoid compound extracted from Scutellaria baicalensis (Huang-Qin or Chinese skullcap) and has been studied thoroughly by many researchers till date for its anti-viral, anti-oxidant, anti-cancerous and neuro-protective properties. Numerous experiments conducted in vitro and in vivo have demonstrated wogonin's excellent tumor i...

This study was performed to identify the non- synonymous polymorphisms in the myosin heavy chain 1 gene (MYH1) association with skeletal muscle development in economically important Jeju Native Pig (JNP) and Berkshire breeds. Herein, we present an in silico analysis, with a focus on (a) in silico approaches to predict the functional effect of non-s...

The athletic abilities of the horse serve as a valuable model to understand the physiology and molecular mechanisms of adaptive responses to exercise. We analyzed differentially expressed genes in triceps brachii muscle tissues collected from Eonjena Taeyang and Jigusang Seryeok Thoroughbred horses and their co-expression networks in a large-scale...

Lipocalin-2 (LCN2) is a 25-kDa protein in the lipocalin family that shows antibacterial activity. The aim of this study was to clone human LCN2 into the mammalian-specific pIRES2-AcGFP1 vector and to determine the antibacterial activity of the recombinant pIRES2-AcGFP1-hLCN2 plasmid against major bacterial pathogens that cause bovine mastitis. The...

In pig breeding, semen of pedigreed boars carries a vast economic importance. The present analytical study was planned at Jeju Island, South Korea. In the study, 74 boars of age group between 1 to 2 years of Landrace, Yorkshire, Duroc and Berkshire breeds were included. The season around the year and breed of the boar are the determinants which aff...

Tumor initiating cancer stem-like cells (TICSCs) have recently become the object of intensive study. Human-Lipocalin-2 (hLCN2) acts as a biomarker for cancers. The aim of the present study was to explore new insights regarding the potential role of LCN2 in inducing epithelial to mesenchymal transition (EMT) by transfecting LCN2 into CD133+-A549-TIC...

Fermentation of meat is a traditional energy efficient preservation method widely used for improving quality, functionality, keeping quality and shelf life of fermented meat products. Fermentation of meat causes number of physical, biochemical and microbial changes, which eventually imparting functional properties, sensory characteristics and nutri...

Curcumin is a natural polyphenol and essential curcuminoid derived from the rhizome of the medicinal plant Curcuma longa (L.) is universally acknowledged as "Wonder drug of life". It is a vital consumable and restorative herb, commonly keened for several ailments such as cancer, arthritis, pain, bruises, gastrointestinal quandaries, swelling and mu...

Abstract: The monomorphism of newly hatched chicks is a major hurdle for poultry farmers at the time of sexing. The traditional sexing techniques are inaccurate and time-consuming. Therefore, the present study aimed to develop a loop-mediated isothermal amplification (LAMP)-based sex determination method to obtain a higher separation accuracy rate...

Recent evidence demonstrated that Lipocalin 2 (LCN2) is garnering interest from a wide spectrum as biomarker. Here, we present an in silico characterization of LCN2 belonging to prominent organisms focusing for their physicochemical and structural differences. We found significant variations in physicochemical properties between organisms and low s...

The nuclear factor κB (NF-κB) and interleukin-6 (IL-6) contribute to multidrug resistance (MDR) in tumor chemotherapy. The essential phenomenon of oncogenic activation of NF-κB in cancer-initiating cells showing MDR resulting from increased IL-6 expression is still unclear. Cancer stem cells (CSCs) have been the objective of intensive study. The ai...

This study was carried out with the objective to investigate the differentially expressed genes (DEGs) between Jeju native pig (JNP) and Berkshire piglets. The RNA-Seq technique was used to investigate the transcriptomes in the fat, liver and longissimus dorsi muscle from these two breeds. Paired-end reads of the sequences that passed the quality f...

Background Mesenchymal stem cells (MSCs) are considered an attractive approach for gene or drug delivery in cancer therapy. Thus, in this study, the ability of human bone marrow-derived MSCs expressing the cytosine deaminase/5-fluorocytosine prodrug (CD/5-FC MSCs) to target the human osteosarcoma cell line Cal72 was evaluated.Methods The stable CD/...

The undigested phytates excreted through the feces causes the environmental pollution. The phytase enzyme in feed for layers increases the bioavailability of phosphorus, reduces its excretion and is also reported to be a valuable source of pigment for meat or egg. The objective of this study was to assess the effect of supplementation of phytase ex...

Phenotypic characterization and body biometric in 13 traits (height at withers, body length, chest girth, paunch girth, ear length, tail length, length of tail up to switch, face length, face width, horn length, circumference of horn at base, distances between pin bone and hip bone) were recorded in 233 adult Gojri buffaloes from Punjab and Himacha...

Human, murine and monkey spermatogonial stem cells (SSCs) have the capability to undergo self-renewal and differentiation into different body cell types in vitro, which are expected to serve as a powerful tool and resource for the developmental biology and regenerative medicine. We have successfully isolated and characterized the chicken SSCs from...

A total of 350 samples 50 each from raw poultry meat, poultry cloacal swabs and human diarrheic cases, besides 200 eggs were processed for the isolation of Salmonella. Effect of varying temperature and pH on growth of Salmonella isolates was evaluated by growing the organism in Brain Heart Infusion Broth at 4, 10, and 30°C with pH values of 4.5, 5....

The study was conducted to record the prevalence of allergic dermatitis in canine amongst the dermatitis cases presented at the Veterinary Clinics and Teaching Hospital, F.V.Sc & A.H., R.S Pura, Central Veterinary Hospital Talab Tillo in the Jammu region. The diagnosis was done by intradermal skin testing, IgE level, clinical score status on the ba...