Navid Kashaninejad

Navid Kashaninejad
Griffith University · Queensland Micro- and Nanotechnology Centre

BSc, MSc, PhD

About

75
Publications
78,020
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1,232
Citations
Additional affiliations
January 2009 - July 2013
Nanyang Technological University
Position
  • PhD Student

Publications

Publications (75)
Article
Electrical neuron stimulation holds promise for treating chronic neurological disorders, including spinal cord injury, epilepsy, and Parkinson’s disease. The implementation of ultrathin, flexible electrodes that can offer noninvasive attachment to soft neural tissues is a breakthrough for timely, continuous, programable, and spatial stimulations. W...
Article
Full-text available
Separation and detection of cells and particles in a suspension are essential for various applications, including biomedical investigations and clinical diagnostics. Microfluidics realizes the miniaturization of analytical devices by controlling the motion of a small volume of fluids in microchannels and microchambers. Accordingly, microfluidic dev...
Article
Full-text available
Keeping the oxygen concentration at the desired physiological limits is a challenging task in cellular microfluidic devices. A good knowledge of affecting parameters would be helpful to control the oxygen delivery to cells. This study aims to provide a fundamental understanding of oxygenation process within a hydrogel-based microfluidic device cons...
Article
Full-text available
Current chemotherapies can often kill fast-growing cancer cells, but are ineffective in destroying cancer stem cells (CSCs). This study aimed to investigate the effect of in vitro cell culture conditions on the features of lung adenocarcinoma cells (represented by A549, non-small cell type (NSCLC), cell lines) and the induction of cancer stem-like...
Preprint
Electrical neuron stimulation holds promise for the treatment of several chronic neurological disorders, including spinal cord injury, epilepsy, and Parkinson’s disease. The implementation of ultrathin, flexible electrodes, that can offer non-invasive attachment to soft neural tissues, is a breakthrough technology for timely, continuous, programabl...
Chapter
Micro/nanofluidic drug delivery systems have attracted significant attention as they offer unique advantages in targeted and controlled drug delivery. Based on the desired application, these systems can be categorized into three different groups: in vitro, in situ and in vivo microfluidic drug delivery platforms. In vitro microfluidic drug delivery...
Article
DOI: 10.1002/elps.202100126 The cover picture shows a sheathless three‐dimensional viscoelastic focusing of white blood cells, red blood cells, and cancer cells in a zigzag microchannel. Cell focusing is essential for the downstream cell separation, detection, counting or analysis. In general, an extended channel length is required to achieve effic...
Article
Full-text available
Rapid isolation of white blood cells (WBCs) from whole blood is an essential part of any WBC examination platform. However, most conventional cell separation techniques are labor-intensive and low throughput, require large volumes of samples, need extensive cell manipulation, and have low purity. To address these challenges, we report the design an...
Article
Full-text available
The up-and-coming microfluidic technology is the most promising platform for designing anti-cancer drugs and new point-of-care diagnostics. Compared to conventional drug screening methods based on Petri dishes and animal studies, drug delivery in microfluidic systems has many advantages. For instance, these platforms offer high-throughput drug scre...
Preprint
Full-text available
Rapid isolation of white blood cells (WBCs) from whole blood is an essential part of any WBC examination platform. However, most conventional cell separation techniques are labor-intensive and low throughput, require large volumes of samples, need extensive cell manipulation, and have low purity. To address these challenges, we report the design an...
Preprint
Full-text available
The up-and-coming microfluidic technology is the most promising platform for designing anti-cancer drugs and new point-of-care diagnostics. Compared to conventional drug screening methods based on Petri dishes and animal studies, drug delivery in microfluidic systems has many advantages. For instance, these platforms offer high throughput drug scre...
Article
Full-text available
Microfluidic lab-on-chip devices are widely being developed for chemical and biological studies. One of the most commonly used types of these chips is perfusion microwells for culturing multicellular spheroids. The main challenge in such systems is the formation of substantial necrotic and quiescent zones within the cultured spheroids. Herein, we p...
Article
Microfluidic particle focusing has been a vital prerequisite step in sample preparation for downstream particle separation, counting, detection or analysis, and has attracted broad applications in biomedical and chemical areas. Besides all the active and passive focusing methods in Newtonian fluids, particle focusing in viscoelastic fluids has been...
Article
Full-text available
DNA methylation is a cell-type-specific epigenetic marker that is essential for transcriptional regulation, silencing of repetitive DNA and genomic imprinting. It is also responsible for the pathogenesis of many diseases, including cancers. Herein, we present a simple approach for quantifying global DNA methylation in ovarian cancer patient plasma...
Preprint
Full-text available
Microfluidic lab-on-chip devices are widely being developed for chemical and biological studies. One of the most commonly used types of these chips is perfusion microwells for culturing multicellular spheroids. The main challenge in such systems is the formation of substantial necrotic and hypoxic zones within the cultured spheroids. Herein, we pro...
Article
Full-text available
Wearable and implantable bioelectronics have experienced remarkable progress over the last decades. Bioelectronic devices provide seamless integration between electronics and biological tissue, offering unique functions for healthcare applications such as real-time and online monitoring and stimulation. Organic semiconductors and silicon-based flex...
Article
Full-text available
Dermal interstitial fluid (ISF) is a novel source of biomarkers that can be considered as an alternative to blood sampling for disease diagnosis and treatment. Nevertheless, in vivo extraction and analysis of ISF are challenging. On the other hand, microneedle (MN) technology can address most of the challenges associated with dermal ISF extraction...
Article
Full-text available
Intracellular delivery is considered an indispensable process for various studies, ranging from medical applications (cell-based therapy) to fundamental (genome-editing) and industrial (biomanufacture) approaches. Conventional macroscale delivery systems critically suffer from such issues as low cell viability, cytotoxicity, and inconsistent materi...
Article
Full-text available
In article number 2005363, Majid Ebrahimi Warkiani and co‐workers comprehensively review the existing intracellular delivery technologies at the macro‐ and micro‐/nanoscale based on their impact resolution. This study gives an insight to researchers interested in intracellular delivery, ranging from biologists looking for the most appropriate cargo...
Preprint
Full-text available
Dermal interstitial fluid (ISF) is a novel source of biomarkers that can be considered as an alternative to blood sampling for disease diagnosis and treatment. Nevertheless, in vivo extraction and analysis of ISF are challenging. On the other hand, microneedle (MN) technology can address most of the challenges associated with dermal ISF extraction...
Article
Full-text available
Fluid shear stress, osmotic pressure gradient, and extracellular matrix stiffness are invivo, mechanical stimuli that affect cells’ interaction with drugs in biological structures. This paper introduces an integrated concentration gradient generator (CGG) capable of providing cell monolayers with these stimuli and demonstrates the procedures for de...
Article
In article number 2000108, Navid Kashaninejad, Nam‐Trung Nguyen, and co‐workers overcome the bottleneck of evaporation in liquid marbles (LMs) by incorporating an agarose hydrogel inside the LMs. The findings show that LMs with hydrogel prolong the lifetime of the platform and leads to the formation of perfect multicellular spheroids.
Article
Digital microfluidics based on liquid marble (LM) has recently emerged as a promising platform for liquid handling and cell‐based assays. However, evaporation is a critical problem in such platforms, hindering their wide‐range applications in various fields. This study aims to develop a functional sessile LM system for long‐term 3D cell culture. Pr...
Article
Full-text available
Elucidating the microscale interaction between magnetism and fluid flow is of great importance for designing micro-magnetofluidic gradient generators, micromixers, and particle sorters. Co-flowing magnetic and non-magnetic fluids can lead to instability at their interface and subsequent rapid mixing. The mismatch in the magnetisation of the fluids...
Article
Full-text available
Long non-coding RNA HOX transcript antisense intergenic RNA (HOTAIR) is one of the promising biomarkers that has widely been used in determining the stages of many cancers, including ovarian cancer. In cancer diagnostics, the two key analytical challenges for detecting long non-coding RNA biomarkers are i) the low concentration levels (nM to fM ran...
Article
Full-text available
Microfluidics has been demonstrating enormous potential through its role in recent advances in biological sciences. However, designing a new and customized microfluidic platform, gaining a better understanding of its function and the underlying physics still pose significant technical challenges. On the one hand, experimental approaches have been c...
Article
Full-text available
Liver cancer, especially hepatocellular carcinoma (HCC), is an aggressive disease with an extremely high mortality rate. Unfortunately, no promising markers are currently available for the early diagnosis of this disease. Thus, a reliable biomarker reflecting the early behaviour of the tumour will be valuable for diagnosis and treatment. The Ras ho...
Article
Microfluidic concentration gradient generators (μCGGs) are indispensable parts of many emerging lab-on-a-chip platforms for biological studies and drug delivery applications. Most of the μCGGs reported in the literature can only generate the desired concentration gradients in a micron-sized sample (e.g., cells). As such, there is an unmet need to d...
Article
Full-text available
Droplet-based microfluidics offers significant advantages, such as high throughput and scalability, making platforms based on this technology ideal candidates for point-of-care (POC) testing and clinical diagnosis. However, the efficiency of co-encapsulation in droplets is suboptimal, limiting the applicability of such platforms for the biosensing...
Article
Full-text available
Controlling the evaporation process of a droplet is of the utmost importance for a number of technologies. Also, along with the advances of microfabrication, micropatterned surfaces have emerged as an important technology platform to tune the wettability and other surface properties of various fundamental and applied applications. Among the geometr...
Article
Full-text available
In recent years, a new group of nanomaterials named as nanozymes that exhibit enzyme-mimic catalytic activity, has emerged as a promising alternative to natural enzymes. Nanozymes can address some of the intrinsic limitations of natural enzymes such as high cost, low stability, difficulty in storage, and specific working conditions (i.e., narrow su...
Chapter
This chapter discusses the integrations of the nanoparticles with microfluidic platforms for biological diagnosis. It evaluates the applications of two emerging formats of microfluidics, digital microfluidics and paper‐based microfluidics, for biological diagnosis. Microfluidics is an enabling technology that can be employed in numerous biological...
Article
Concentration gradient generators (CGGs) help biologists to perform large scale, fast and high-throughput experiments. This paper introduces a design tool called Tree-like Concentration gradient generator Design Tool (TCDT). The performance of this tool is validated both numerically and experimentally. Three CGGs were fabricated using three differe...
Article
Full-text available
No matter how sophisticated the structures are and on what length scale the pore sizes are, fluid displacement in porous media can be visualized, captured, mimicked and optimized using microfluidics. Visualizing transport processes is fundamental to our understanding of complex hydrogeological systems, petroleum production, medical science applicat...
Article
In article number 1900425, Majid Ebrahimi Warkiani and co‐workers report the use of a newly developed 3D printing resin for rapid softlithography of microfluidic chips, reducing the total design‐to‐development time from several days to less than 5 hrs. This could open new opportunities for biologists and scientists with minimum knowledge about micr...
Preprint
Full-text available
Fluid mechanics of flow in hydrophobic, rectangular microchannels with finite aspect ratios is of paramount importance. In such microchannels, not only the effect of the side walls should be taken into account, but also the classical assumption of no-slip boundary condition (BC) is no longer valid at the solid-liquid interface. Accordingly, slip fl...
Article
Full-text available
Microfluidic cell culture platforms are ideal candidates for modeling the native tumor microenvironment because they can precisely reconstruct in vivo cellular behavior. Moreover, mathematical modeling of tumor growth can pave the way toward description and prediction of growth pattern as well as improving cancer treatment. In this study, a modifie...
Article
Polydimethylsiloxane (PDMS) has long been a matter of great interest in microfluidics due to its unique features. For instance, PDMS biocompatibility allows investigators to perform cell-based assay while its transparency facilitates real-time monitoring. PDMS coating also enables researchers to perform their experiments in the presence of organic...
Article
Full-text available
This study presents a new form of velocity distribution in laminar liquid flow in rectangular microchannels using the eigenfunction expansion technique. Darcy friction factor and Poiseuille number are also obtained analytically. Due to the symmetry of the solutions, the effects of changing the aspect ratio from 0 to ∞ are also discussed. Using fini...
Article
Autoantibodies produced by the patients' own immune systems in response to foreign substances are emerging as an attractive biomarker for early detection of cancer. These serum immunobiomarkers are produced in large quantities despite the presence of very less amount of the corresponding antigens, and thus presenting themselves as a novel class of...
Article
Electronic supplementary information (ESI) available. ABSTRACT: Dielectrophoresis is a robust approach for manipulating bio-particles in microfluidic devices. In recent years, many groups have developed dielectrophoresis-based microfluidic systems for separation and concentration of various types of bio-particles, where the gradient of the electric...
Article
High-throughput, rapid and homogeneous mixing of microdroplets in a small length scale such as that in a microchannel is of great importance for lab-on-a-chip applications. Various techniques for mixing enhancement in microfluidics have been extensively reported in the literature. One of these techniques is the mixing enhancement with magnetofluidi...
Article
Full-text available
Tumor metastasis is responsible for the vast majority of cancer-associated morbidities and mortalities. Recent studies have disclosed the higher metastatic potential of circulating tumor cell (CTC) clusters than single CTCs. Despite long-term study on metastasis, the characterizations of its most potent cellular drivers, i.e., CTC clusters have onl...
Article
Cryopreservation without cryoprotectant remains a significant challenge for the reestablishment of cell culture after freeze-thaw. Thus, finding an alternative and simple cryopreservation method is necessary. Liquid marble (LM) based digital microfluidics is a promising approach for cryoprotectant-free cryopreservation. However, the use of this pla...
Conference Paper
Full-text available
We report a unique and robust bioreactor platform based on liquid marble (LM) with embedded hydrogel for digital microfluidics. The LM is a liquid droplet coated with hydrophobic powder. To prevent this droplet from evaporation, the LM floats on a liquid surface. Furthermore, a hydrogel loaded with growth factors and drugs is embedded in the LM. Th...
Article
There has been increasing interest in electronic systems with integrated microfluidic active cooling modules. However, the failure of 3C-SiC/Si interface with increasing temperature has prevented the development of 3C-SiC power electronic devices. Here, we develop all integrated transparent heating, sensing and cooling systems on a single silicon c...
Article
Full-text available
Three-dimensional (3D) cell culture systems can be regarded as suitable platforms to bridge the huge gap between animal studies and two-dimensional (2D) monolayer cell culture to study chronic diseases such as cancer. In particular, the preclinical platforms for multicellular spheroid formation and culture can be regarded as ideal in vitro tumour m...
Article
Full-text available
Three-dimensional (3D) cell culture systems can be regarded as suitable platforms to bridge the huge gap between animal studies and two-dimensional (2D) monolayer cell culture to study chronic diseases such as cancer. In particular, the preclinical platforms for multicellular spheroid formation and culture can be regarded as ideal in vitro tumor mo...
Article
Thin, porous membranes are important components in a microfluidic device, serving as separators, filters, and scaffolds for cell culture. However, the fabrication and the integration of these membranes possess many challenges, which restrict their widespread applications. This paper reports a facile technique to fabricate robust membrane-embedded m...
Article
Full-text available
Microfluidic devices have been widely used for biological and cellular studies. Microbioreactors for three-dimensional (3D) multicellular spheroid culture are now considered as the next generation in in vitro diagnostic tools. The feasibility of using 3D cell aggregates to form multicellular spheroids in a microbioreactor with U-shaped barriers has...
Article
A cell spheroid is a three-dimensional (3D) aggregation of cells. Synthetic, in-vitro spheroids provide similar metabolism, proliferation, and species concentration gradients to those found in-vivo. For instance, cancer cell spheroids have been demonstrated to mimic in-vivo tumor microenvironments, and are thus suitable for in-vitro drug screening....
Article
Full-text available
A microfluidic system provides an excellent platform for cellular studies. Most importantly, a three-dimensional (3D) cell culture model reconstructs more accurately the in vivo microenvironment of tissue. Accordingly, microfluidic 3D cell culture devices could be ideal candidates for in vitro cell culture platforms. In this paper, two types of 3D...
Article
Full-text available
Micro and nanotechnology can potentially revolutionize drug delivery systems. Novel microfluidic systems have been employed for the cell culture applications and drug delivery by micro and nanocarriers. Cells in the microchannels are under static and dynamic flow perfusion of culture media that provides nutrition and removes waste from the cells. T...
Article
Full-text available
The fields of assisted reproductive technology (ART) and in vitro fertilization (IVF) have progressed rapidly, yet still need further improvements. Microfluidic technology can incorporate various ART procedures such as embryo/gamete (sperm/oocyte) analysis, sorting, manipulation, culture, and monitoring. The introduction of paper-based and droplet-...
Article
Full-text available
This paper reports the fabrication of electrospun polydimethylsiloxane (PDMS) membranes/scaffolds that are suitable for three-dimensional (3D) cell culture. Through modification the ratio between PDMS and polymethylmethacrylate (PMMA) as carrier polymer, we report the possibility of increasing PDMS weight ratio of up to 6 for electrospinning. Incre...
Article
Full-text available
The interdisciplinary research field of microfluidics has the potential to revolutionize current technologies that require the handling of a small amount of fluid, a fast response, low costs and automation. Microfluidic platforms that handle small amounts of liquid have been categorised as continuous-flow microfluidics and digital microfluidics. Th...
Conference Paper
Full-text available
امروزه بیماري سرطان سهم بالایی از مرگ و میر انسانها را در سراسر جهان در بر میگیرد بنابراین مقابله با این بیماري و تلاش براي درمان و پیشگیري آن از اهمیت بالایی برخوردار است. یک ابزار که میتواند در تشخیص و درمان سرطان نقش بهسزایی ایفا کند، ایجاد محیط کشت سهبعدي سلولهاي سرطانی است. اما محیطهاي کشت سهبعدي معمولی، نمیتوانند به خوبی شرایط و ویژگیهاي درون...