Natwar Jha

• B.sc, M.sc. Ph.D. Biotechnology
• Researcher at Pondicherry University

In this study, fermented rice gruel, a traditional food in South Telangana, India, was used as a source for the isolation of biologically significant probiotics. A total of 56 morphologically diverse strains were isolated and screened for their ability to inhibit dental biofilms as well as foodborne and aquaculture bacterial pathogens. Among these,...

The goal of this research was to determine the effect of dietary supplementation of vitamin C-loaded chitosan nanoparticles (VC-CSNPs) on the antioxidant activity, disease resistance, non-specific immunity, and gene expression of the whiteleg shrimp Litopenaeus vannamei which was fed for eight weeks and challenged with Vibrio harveyi. Antioxidants...

In this study, we optimized a simple method of cryopreservation for Mugil cephalus sperm based on post-thaw motility and viability. A series of experiments were conducted by changing the extender, cryoprotectant and freezing height above the liquid nitrogen (LN) surface. First, we carried out the cryopreservation using the extender V2E and cryoprot...

Rhizophora mucronata Leaves Polysaccharide (RMLP) was extracted and purified from the mangrove Rhizo-phora mucronata leaves by anion-exchange and size-exclusion column chromatography. Physicochemical characterization of RMLP was evaluated by spectrtoscopic analysis (FT-IR, 1 H & 13 C NMR) and RP-HPLC. The molecular weight of RMLP was found to be 2....

The Box-Behnken design was applied to determine the optimal parameters of the extraction condition by using the response surface methodology (RSM) from the leaves of Sonneratia caseolaris L. The result indicates the best-optimized conditions used for the extraction of polysaccharides at 84.02 °C temperature, 3.12 h time, and 27.31 mL/g for the wate...

The selenium nanoparticles (SeNPs) linked with exopolysaccharide (EPS) of E. faecium MC-5 were successfully produced. UV–Vis spectra, DLS, zeta potential, SEM, TEM, FT-IR, thermogravimetric analysis-DSC, and X-ray diffraction measurements were used to characterize the SeNPs in terms of formation, size, stability, and functional group attachment, mo...

E. faecium MC-5 was isolated and characterized from the gut content of C. carpio and supplemented with a pellet diet at the ratio of 1.0 × 10⁶, 1.0 × 10⁹, and 1.0 × 10¹² cfu g–1. Then it was fed to Cirrhinus mrigala for 42 days and the growth performances were evaluated. The growth performance of experimental groups was significantly (P < 0.05) inc...

Background Selenium (Se) is considered an important micronutrient for human and animal health having potential biological functions and it is required for the prevention and treatment of diseases. The present study was designed to optimize the synthesis conditions of selenium nanoparticles by the Taguchi design method using sodium selenite as a pre...

The goal of this study was to determine the unique characteristics of Enterococcus faecium MC-5, a probiotic bacteria isolated from the intestine of a fish, Cyprinus carpio specularis, collected from Dal Lake in Srinagar, Kashmir, India. For this, the important valuable probiotic attributes, some functional properties, and safety assessments were a...

A gene encoding lipase enzyme from Bacillus flexus PU2 was cloned and expressed in E. coli BL21 (DE3) pLysS and purified protein having the molecular weight of 34 kDa. This lipase was found to be alkaline (pH 9) and slightly thermophilic. This lipase was observed to retain its activity in the presence of methanol, ethanol, DMSO, and acetone. Ferrou...

In this study, we developed a simple and robust method of cryopreservation of sperm in Mugil cephalus. A series of experiments were conducted to develop a simplified method by changing the extender, cryoprotectant and freezing height above the liquid nitrogen (LN2) surface. Fresh sperm were diluted 1:1 in a cryomedium (cryomedium = extender + cryop...

Avicennia marina mangrove leaves polysaccharide (AMLP) was used for the synthesis of polysaccharide-based selenium (AMLP-SeNPs) and silver nanoparticles (AMLP-AgNPs). The synthesized nanoparticles were further characterized by UV-Vis, DLS, FT-IR, X-ray diffraction, and HR-TEM analysis. A 60-day (8 weeks) feeding trial experiment was conducted to in...

The lipase gene from Psychrobacter celer PU3 was cloned into pET-28a(+) expression vector and overexpressed in E. coli BL21 (DE3) pLysS cells. The purified Psychrobacter celer lipase (PCL) was characterized as an alkaline active enzyme and has a molecular mass of around 30 kDa. The PCL was active even at a low temperature and the optimum range was...

In the present study, Enterococcus faecium MC-5 isolated from the fish gut was cultured in the MRS broth to produce exopolysaccharide (EPS). The maximum EPS production was optimized using Response Surface Methodology (RSM) with three different factors which include sucrose, ammonium nitrate and pH. RSM result predicted that maximum EPS (16.48 g/L)...

The present work is carried out to determine the biodegradation potential of n-hexadecane by bacterial strain P. aeruginosa PU1 isolated from soil polluted with transformer oil. The bacteria showed its ability to grow in n-hexadecane containing medium and its utilization was revealed by a 2, 6-dichlorophenol indophenol assay, where complete decolou...

A low molecular weight polysaccharide, the AMLP was isolated from the leaves of Avicennia marina and sequentially purified using DEAE-Sepharose fast flow and size exclusion chromatography. Monosaccharide analysis suggested that AMLP is mainly composed of fucose (38.34 %), galactose (30.16 %), mannose (13.32 %), and rhamnose (18.18 %). IR spectrosco...

Aim: The present study aims to evaluate the potential antioxidant and antiproliferative properties of probiotic bacterial isolates Weissella cibaria p3B, Bacillus subtilis CS, and Bacillus tequilensis CL, isolated from South Indian fermented tomato pickle (homemade) and gut content of indigenous country chicken. Methods and results: The bacteria...