Natsaran Saichana

Mae Fah Luang University | MFU · School of Science

Quaternized chitosan (QCS) was blended with pectin (Pec) to improve water solubility and antibacterial activity of the hydrogel films. Propolis was also loaded into hydrogel films to improve wound healing ability. Therefore, the aim of this study was to fabricate and characterize the propolis-loaded QCS/Pec hydrogel films for use as wound dressing...

Lophiostomataceae species commonly occur as saprobes on twigs, stems or bark on diverse woody and herbaceous plants in aquatic and terrestrial habitats. In the present study, we introduce Lophiostoma khanzada-kirgizbaeva sp. nov. and Paucispora xishanensis sp. nov. as new species isolated from woody substrates from Uzbekistan and China, respectivel...

Grasses are agriculturally, economically and ecologically dominant among plants. Their fungal diversity is fascinating but has been relatively poorly studied or restricted to a few important hosts or pathogens. Therefore, numerous grass-inhabiting fungal species are awaiting discovery or re-study based on morphology and molecular phylogeny. In an o...

We identified a novel flavoprotein-cytochrome c complex D-gluconate dehydrogenase (GADH) encoded by gndXYZ of Gluconobacter oxydans NBRC 3293, which is phylogenetically distinct from previously reported GADHs encoded by gndFGH and gndSLC of Gluconobacter spp. To analyze the biochemical properties of respective GADHs, Gluconobacter japonicus NBRC 32...

Botryosphaeriaceae species are associated with grapevine trunk diseases. Lasiodiplodia theobromae plays a significant role as one of the most virulent grapevine pathogens in the world. In this study, we evaluate the effects of temperature on phytotoxin‐induced virulence in Lasiodiplodia theobromae. The fungal strain is grown in Czapek‐Dox liquid me...

Vinegar is fermented product from alcoholic and acetic acid fermentation which can be made from various material such as crops, fruits and honey. Microorganisms, yeast and AAB, and substrate are key for vinegar production which can determine the quality and production time of vinegar. This study aims to use the isolated yeast and AAB from homemade...

Two common types of glutinous rice, Khao Niew Dam (KK) and Khao Niew Kiew Ngu (KN), found in Chiang Rai, Thailand were selected to study the feasibility to be the raw material for vinegar production. The malting process is a key role to gain the sufficient sugar content for wort preparation which is used as the substrate for the vinegar fermentatio...

A stinkhorn fungus was collected from the mountainous area of Yoshida campus, Yamaguchi University, Japan. Morphological characterization and similarity of large subunit ribosomal DNA sequences identified the fungus as Pseudocolus fusiformis. MonoTrap™ was combined with gas chromatography-mass spectrometry (GC-MS) to identify volatile organic compo...

Ability of poly (styrene- alt -maleic anhydride) (PSMA) to undergo a conformational transition into an amphipathic α -helix coil offers one possible mechanism by which PSMA surface activity can be switched on or off in response to the pH change. This behaviour allows it to be useful in membrane solubilization for extraction technology. Bioactive co...

An updated checklist of microfungi, excluding bambusicolous fungi, recorded on grasses from Thailand is provided. The host plant(s) from which the fungi were recorded in Thailand is given. Those species for which molecular data is available is indicated. In total, 172 species and 35 unidentified taxa have been recorded. They belong to the main taxo...

Bacterial cellulose (BC) is a biological material, which is naturally produced by acetic acid bacteria (AAB). Bacterial cellulose has been used as an agent in various industrial applications, such as food, biomaterial, cosmetic and medical industries. The aim of this study was to use low-cost materials to investigate the optimum condition to produc...

Bacterial cellulose (BC) is widely used in various applications. To apply in biomedical use, the natural BC and low-cost production should be considered. The aim of this study was to apply the spent coconut water (SCW) as supplement in the culture medium to produce high yield of BC. An acetic acid bacterium, Komagataeibacter nataicola (formerly Ace...

A Truncatella-like coelomycete fungus was isolated from dead branches of Cytisus and Helichrysum species from Arezzo Province, Italy. DNA sequence analyses of a combined LSU, ITS and SSU dataset indicated a close phylogenetic affinity of our isolate to Hyponectria buxi (Hyponectriaceae). Herein we describe it as a new genus with Pseudotruncatella a...

Table S1. PCR primers used for construction of PqqE mutant enzymes

PqqE is a radical S-adenosyl-l-methionine (SAM) enzyme that catalyzes the initial reaction of pyrroloquinoline quinone (PQQ) biosynthesis. PqqE belongs to the SPASM (subtilosin/PQQ/anaerobic sulfatase/mycofactocin maturating enzymes) subfamily of the radical SAM superfamily and contains multiple Fe-S clusters. To characterize the Fe-S clusters in P...

This book, written by leading international authorities in the field, covers all the basic and applied aspects of acetic acid bacteria. It describes the importance of acetic acid bacteria in food industry by giving information on the microbiological properties of fermented foods as well as production procedures. Special attention is given to vinega...

Methylobacterium extorquens AM1 is an aerobic facultative methylotroph known to secrete pyrroloquinoline quinone (PQQ), a cofactor of a number of bacterial dehydrogenases, into the culture medium. To elucidate the molecular mechanism of PQQ biosynthesis, we are focusing on PqqE which is believed to be the enzyme catalyzing the first reaction of the...

Acetic acid bacteria are gram-negative obligate aerobic bacteria assigned to the family Acetobacteraceae of Alphaproteobacteria. They are members of the genera Acetobacter, Gluconobacter, Gluconacetobacter, Acidomonas, Asaia, Kozakia, Swaminathania, Saccharibacter, Neoasaia, Granulibacter, Tanticharoenia, Ameyamaea, Neokomagataea, and Komagataeibac...

In vitro adaptation is one of the most challenging subjects in biology to understand adaptive evolution. Microbial adaptation to temperature is not only interesting in terms of understanding the adaptation mechanism, but also useful for industrial applications. In this study, we attempted the in vitro adaptation of Acetobacter pasteurianus SKU1108...

l-Sorbose reductase from Gluconobacter frateurii (SR) is an NADPH-dependent oxidoreductase. SR preferentially catalyzes the reversible reaction between d-sorbitol and l-sorbose with high substrate specificity. To elucidate the structural basis of the catalytic mechanism and the substrate specificity of SR, we have determined the structures of apo-S...

Further upstream of sldSLC, genes for FAD-dependent D-sorbitol dehydrogenase in Gluconobacter frateurii, three additional genes (sldR, xdhA, and perA) are found: for a transcriptional regulator, NAD(P)-dependent xylitol dehydrogenase, and a transporter protein, a member of major facilitator superfamily, respectively. xdhA and perA but not sldR were...

Acetobacter pasteurianus is a Gram-negative strictly aerobic bacterium that is widely used for the industrial production of vinegar. Three Acetobacter pasteurianus strains, SKU1108, NBRC 3283 and IFO 3191, have the same 16S rRNA sequence (100 % sequence identity) but show differences in thermotolerance. To clarify the relationships between phylogen...

l-Sorbose reductase from Gluconobacter frateurii (SR) is an NADPH-dependent oxidoreductase. SR preferentially catalyzes the reversible reaction between d-sorbitol and l-sorbose with high substrate specificity. To elucidate the structural basis of the catalytic mechanism and the substrate specificity of SR, we have determined the structures of apo-S...

We isolated thermotolerant Gluconobacter strains that are able to produce 5-keto-d-gluconic acid (5KGA) at 37°C, a temperature at which regular mesophilic 5KGA-producing strains showed much less growth and 5KGA production. The thermotolerant strains produced 2KGA as the major product at both 30 and 37°C. The amount of ketogluconates produced at 37°...

For easy measurement of 5-keto D-gluconate (5KGA) and 2-keto D-gluconate (2KGA), two enzymes, 5KGA reductase (5KGR) and 2KGA reductase (2KGR) are useful. The gene for 5KGR has been reported, and a corresponding gene was found in the genome of Gluconobacter oxydans 621H and was identified as GOX2187. On the other hand, the gene for 2KGR was identifi...

Most Gluconobacter species produce and accumulate 2-keto-d-gluconate (2KGA) and 5KGA simultaneously from d-glucose via GA in culture medium. 2KGA is produced by membrane-bound flavin adenine dinucleotide-containing GA 2-dehydrogenase (FAD-GADH). FAD-GADH was purified from “Gluconobacter dioxyacetonicus” IFO 3271, and N-terminal sequences of the thr...