Nathalie Mignet

Nathalie Mignet
CNRS Université de Paris INSERM

PhD

About

178
Publications
24,258
Reads
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3,249
Citations
Citations since 2016
95 Research Items
2045 Citations
20162017201820192020202120220100200300400
20162017201820192020202120220100200300400
20162017201820192020202120220100200300400
20162017201820192020202120220100200300400
Additional affiliations
January 2014 - December 2015
French Society for Nanomedecine
Position
  • CEO
Description
  • 2014 Upcoming events Joint Meeting SFNano & SCT – April 8, Paris Translational Nanomedicine, Intern., August 27-29, Angers, France Workshop In vitro/in vivo correlation- October 4-6, Porto, Portugal SFNano Annual Meeting 2014, December 9-12, Nancy
January 2004 - December 2013
Université René Descartes - Paris 5
Position
  • Team leader Vectors for molecular imaging and targeted therapy
October 2000 - December 2003
French National Centre for Scientific Research
Position
  • Centre national de la recherche scientifique/Aventis Pharma

Publications

Publications (178)
Article
Monoclonal antibodies (mAbs) represent a dynamic class of biopharmaceutical products, as evidenced by an increasing number of market authorizations for mAb innovator and biosimilar products. Stability studies are commonly performed during product development, for instance, to exclude unstable molecules, optimize the formulation or determine the sto...
Article
Full-text available
We report the formulation, characterization, colloidal stability, and in vitro efficiency of Fisetin nanocrystals stabilized by poloxamer P407. Such nanocrystals present a nanometer scale (148.6 ± 1.1 nm) and a high homogeneity (polydispersity index of 0.17 ± 0.01), with a production yield of 97.0 ± 2.5%. The engineered formulations of nanocrystals...
Article
We present in vivo ultrasound modulated optical tomography (UOT) results on mice, using the persistent spectral hole burning (PSHB) effect in a Tm ³⁺ :YAG crystal. Indocyanine green (ICG) solution was injected as an optical absorber and was clearly identified on the PSHB-UOT images, both in the muscle (following an intramuscular injection) and in t...
Article
Persistent luminescence nanoparticles (PLNPs) are attracting growing interest for non-invasive optical imaging of tissues with a high signal to noise ratio. PLNPs can emit a persistent luminescence signal through the tissue transparency window for several minutes, after UV light excitation before systemic administration or directly in vivo through...
Article
Full-text available
Les techniques de pharmacotechnie actuelles permettent d'améliorer la biodisponibilité, stabilité et efficacité des molécules et par extension innover en termes de stratégies thérapeutiques. Elles peuvent, à titre d'exemple, permettre l'optimisation des doses de substances actives et d'excipients au regard de l'efficacité thérapeutique du produit f...
Article
Liposomes constitute the most exploited drug-nanocarrier with several liposomal drugs on the market. Microfluidic-based preparation methods stand up as a promising approach with high reproducibility and the ability to scale up. In this study, liposomes composed of DOPC, cholesterol, and DSPE-PEG 2000 with different molar ratios were fabricated usin...
Article
Full-text available
Aim: Gene-based immunotherapy against cancer is limited by low gene transfer efficiency. In the literature, interleukin-12 (IL-12) encoding plasmid associated with sonoporation has been shown to enhance antitumoral activity. Moreover, non-viral carriers and high-frequency ultrasound have both been shown to promote immune response activation. Here,...
Article
Full-text available
Pregnant women are still considered as drug orphans. Developing new medications for pregnancy complications is an urgent need. Nanomedicines seem to be a promising approach to control the biodistribution of drugs to ensure both the mother’s and the fetus’ safety. Understanding the interaction between nanoparticles and the placental barrier is a key...
Article
Flavonoids have been considered as promising molecules for cancer treatment due to their pleiotropic properties such as anti–carcinogenic, anti–angiogenic or efflux proteins inhibition. However, due to their lipophilic properties and their chemical instability, vectorization seems compulsory to administer flavonoids. Flavonoids have been co-encapsu...
Article
Full-text available
Pregnancy-associated disorders affect around 20% of pregnancies each year around the world. The risk associated with pregnancy therapeutic management categorizes pregnant women as “drug orphan” patients. In the last few decades, nanocarriers have demonstrated relevant properties for controlled drug delivery, which have been studied for pregnancy-as...
Article
Full-text available
Self-emulsifying microbubbles, especially designed to increase the contrast of ultrasound images by the inclusion of perfluorocarbon molecules, have been studied by thermal analysis techniques. The microbubbles were made of a blend of gas (20%), surfactants (50%) and water (30%). The surfactants were mixtures of polysorbate-85, Span-80, poloxamer 1...
Article
Full-text available
Pregnancy is a delicate state, during which timely investigation of possible physiological anomalies is essential to reduce the risk of maternal and fetal complications. Medical imaging encompasses different technologies to image the human body for the diagnosis, course of treatment management, and follow-up of diseases. Ultrasound (US) is currentl...
Article
Full-text available
Glaucoma is a wide-spread eye disease caused by elevated intraocular pressure. Uncontrolled, this pressure may lead to damages to the optic nerve. Prostaglandin analogues, such as latanoprost and travoprost (which are water-insoluble active substances), are the most used class of active pharmaceutical ingredient. To administer them as eye drops, pr...
Article
Full-text available
Monoclonal antibodies (mAbs) represent a major category of biopharmaceutical products which due to their success as therapeutics have recently experienced the emergence of mAbs originating from different types of trafficking. We report the development of an analytical strategy which enables the structural identification of mAbs in addition to compr...
Article
Full-text available
According to the Center for Disease Control and Prevention (CDC), the coronavirus disease 2019, a respiratory viral illness linked to significant morbidity, mortality, production loss, and severe economic depression, was the third-largest cause of death in 2020. Respiratory viruses such as influenza, respiratory syncytial virus, SARS-CoV-2, and ade...
Article
Objectifs Identification d’anticorps monoclonaux dans des échantillons saisis à l’aide de méthodes analytiques complémentaires permettant de démontrer sans ambiguïté l’identité de la protéine étudiée et de distinguer d’éventuelles traces de dégradations. Méthode Les anticorps monoclonaux (mAbs) connaissent un succès incontestable comme agents thér...
Article
Full-text available
The affinity between functional nanoparticles (NPs) and proteins could determine the efficacy of nanoprobes, nanosensors, nanocarriers, and many other devices for biomedical applications. Therefore, it is necessary to develop analytical strategies to accurately evaluate the magnitude of these protein corona interactions in physiological media. In t...
Article
Full-text available
(1) Background: Glioblastoma (GBM) is the most frequent cerebral tumor. It almost always relapses and there is no validated treatment for second-line GBM. We proposed the coencapsulation of fisetin and cisplatin into liposomes, aiming to (i) obtain a synergistic effect by combining the anti-angiogenic effect of fisetin with the cytotoxic effect of...
Article
FOLFOX is the most common chemotherapy combination prescribed in colorectal cancer. It is composed of calcium levofolinate, 5-fluorouracil and oxaliplatin which demonstrated synergistic outcome. Nowadays, the lack of all-in-one formulation is due to the chemical composition of the pharmaceutical products and the highly pH-dependent stability of eac...
Article
Full-text available
Oxidative stress, which is one of the main harmful mechanisms of pathologies including ischemic stroke, contributes to both neurons and endothelial cell damages, leading to vascular lesions. Although many antioxidants have been tested in preclinical studies, no treatment is currently available for stroke patients. Since cerium oxide nanoparticles (...
Preprint
Full-text available
Oxidative stress, which is one of the main harmful mechanisms of pathologies including is-chemic stroke, contributes to both neurons and endothelial cell damages, leading to vascular lesions. Although many antioxidants have been tested in preclinical studies, no treatment is currently available for stroke patients. Since cerium oxide nanoparticles...
Article
Full-text available
Since its discovery, evidence that siRNA was able to act as an RNA interference effector, led to its acceptation as a novel medicine. The siRNA approach is very effective, due to its catalytic mechanism, but still the limitations of its cellular delivery should be addressed. One promising form of non-viral gene delivery system is liposomes. The var...
Article
One of the main problems of colorectal cancer is not the treatment of the primary tumor but the metastatic stage. Means of metastatic spread is the invasion of the peritoneal cavity which leads to peritoneal metastasis (PM). PM cannot be easily cured, and the current treatments is rather heavy, combining cytoreductive surgery with intravenous and i...
Article
Optical in vivo imaging has become a widely used technique and is still under development for clinical diagnostics and treatment applications. For further development of the field, researchers have put much effort into the development of inorganic nanoparticles (NPs) as imaging probes. In this trend, our laboratory developed ZnGa1.995O4Cr0.005 (ZGO...
Article
Spurred by high risk for local tumor recurrence and non-specific toxicity of systemic chemotherapy, clinicians have recently granted a growing interest to locoregional therapeutic strategies. In this perspective, we recently developed a multipurpose thermosensitive hydrogel based on reversible thermogelling properties of poloxamers P407 and P188, a...
Article
Peritoneum represents a frequent site of metastasis especially for digestive and ovarian primary cancers. The conventional approach to treat peritoneal metastasis consists in systemic chemotherapy, but the median survival associated is only a few months. Recent therapeutic developments result in an aggressive strategy associating cytoreductive surg...
Article
Full-text available
Nanoparticle technology in cancer chemotherapy is a promising approach to enhance active ingredient pharmacology and pharmacodynamics. Indeed, drug nanoparticles display various assets such as extended blood lifespan, high drug loading and reduced cytotoxicity leading to better drug compliance. In this context, organic nanocrystal suspensions for p...
Article
Full-text available
Persistent luminescence nanoparticles (PLNPs) are innovative nanomaterials highly useful for bioimaging applications. Indeed, due to their particular optical properties, i.e., the ability to store the excitation energy before slowly releasing it for a prolonged period of time, they allow in vivo imaging without auto-fluorescence and with a high tar...
Article
Full-text available
Cationic liposomes have been considered as potential vectors for gene delivery thanks to their ability to transfect cells with high efficiency. Recently, the combination of diagnostic agent and therapeutic agents in the same particle to form a theranostic system has been reported. Magnetic liposomes are one of these examples. Due to the magnetic na...
Article
Ascorbic acid is a powerful antioxidant compound involved in many biological functions, and a chronic deficiency is at the origin of scurvy disease. A simple, rapid and cost effective CE method was developed for the separation and simultaneous quantification of ascorbic acid and the major degradation products: dehydroascorbic acid, furfural and fur...
Article
Nanogels are currently considered as promising nanosized drug delivery carriers. Nanogels are made of a crosslinked polymeric network which could encapsulate both hydrophilic and hydrophobic drugs due to their tunable nature. The ability of nanogels to control drug release is vastly described in the literature and researchers are consistently impro...
Article
Full-text available
Mydriasis is required prior to many eye examinations and ophthalmic surgeries. Nowadays, phenylephrine hydrochloride (PHE) and tropicamide (TPC) are extensively used to induce mydriasis. Several pharmaceutic dosage forms of these two active ingredients have been described. However, no optimal therapeutic strategy has reached the market. The present...
Article
Theranostic nanocarriers of antivascular drug encapsulated in thermosensitive ultramagnetic liposomes can be advantageously designed to provide a locally high concentration and an active delivery, with image-guided Magnetic Resonance Imaging (MRI) so as to reliably cure tumor. We propose a novel therapeutic strategy consisting of the magnetic accum...
Article
Protein conjugates such as antibody-drugs conjugates (ADCs) represents the next generation of therapeutic proteins. They allow to combine the biological properties of the protein format with the characteristics of the conjugated ligands. The reaction implemented to couple ligands to the peptide backbone represents a crucial aspect of the production...
Chapter
In the last two decades the development of biomaterials has demonstrated an unprecedented progress which has been emphasized by the introduction of a significant number of innovative materials. As such, the advent of biomaterials represents a formidable opportunity for the implementation of therapeutic treatments, able to induce tissue or organ reg...
Article
The ultimate goal of in vivo imaging is to provide safe tools to probe the inside of a body in order to obtain pathological information, monitor activities, and examine disease progression or regression. In this context zinc gallate doped with chromium III (ZGO) nanoparticles with persistent luminescence properties have been previously developed, t...
Article
Liposomes are nanocarriers composed of phospholipids, especially designed to potentially carry drugs. However, liposomes suffer in terms of leakage of small hydrophilic drugs. To control the release, a system with lipid shell and polymeric viscous core, namely Hybrid liposome/polymer inside (HLPin), has been designed. For this purpose, we setup a s...
Poster
Full-text available
The deleterious role of oxidative stress in neuronal but also vascular lesions following cerebrovascular diseases (including stroke) is clearly established. However, so far no antioxidant strategy has shown clinical efficacy. Cerium oxide nanoparticles (CNPs) have interesting antioxidant capacities via scavenging free radicals and mimicking antioxi...
Article
ntratumoral injection of biocompatible gels is increasingly used for the sustained delivery of drugs and vaccines to enhance the anti-cancer immune response. Granulocyte-macrophage colony stimulating factor (GM-CSF) has become an attractive adjuvant thanks to its ability to boost the antitumor immune response by inducing proliferation, maturation a...
Article
Controlled distribution of a drug by its association to a nanocarrier is a promising approach for the treatment of pregnancy disorders such as preeclampsia. For this application, tracking both the nanocarrier and the drug is necessary to ensure the safety of both the mother and the foetus. This study reports a method to visualize and quantify the u...
Article
Despite the increasing number of clinical trials in gene therapy, no ideal methods still allow non-viral gene transfer in deep tissues such as the liver. We were interested in ultrasound (US)-mediated gene delivery to provide long term liver expression. For this purpose, new positively charged microbubbles were designed and complexed with pFAR4, a...
Article
Lactosylated albumin is currently used as a radiopharmaceutical agent to image the liver asialoglycoprotein receptors and quantify hepatic liver function in various diseases. A lactosylated protein (LACTAL) conjugate showed excellent liver uptake compared to non-lactosylated protein and a high signal to noise ratio, based on the biodistribution in...
Article
Full-text available
Radiofrequency ablation (RFA) of colorectal liver metastases activates a specific T-cell response that is ineffective in avoiding recurrence. Recently, local immunomodulation garnered interests as a way to improve the immune response. We were interested in improving the RFA immune response priming to propose a curative treatment of colorectal cance...
Article
The Cover Feature highlights the importance of drug solid‐state properties for optimized pharmaceutical activity in terms of ex‐ and in vivo stability prior to reaching the targeted cell. For instance, whatever the administration route (e.g., enteral, parenteral), stabilized nanocrystals efficiently cross the extracellular matrix to the cancer cell...
Chapter
Ultrasound-mediated gene delivery is an interesting approach, which could help in increasing gene transfer in deep tissues. Moreover, it allows for performing experiments guided by the image to determine which elements are required. Microbubbles complexed with a eukaryotic expression cassette are excellent agents as they are responsive to ultrasoun...
Chapter
Lipidic vesicles have been extensively studied for their capacity to condensate and deliver nucleic acids to the cells. Many different amphiphilic lipidic structures have been proposed each of them bringing some advances in nonviral gene transfection. The ionic or neutral nature of the lipids induces tremendous differences in the behavior of the co...
Article
The solid form screening of active principal ingredients is a challenge for the pharmaceutical drug development since more than 80% of the marketed drugs are formulated in the solid form. A broad and comprehensive study of the various solid forms of the matter is needed to enhance its translation to clinic. Therefore, the most suitable solid form h...
Article
Background Inflammation involves a heterogeneous macrophage population, for which there is no readily available MR assessment method. Purpose To assess the feasibility of distinguishing proinflammatory M1 and antiinflammatory M2 macrophages at MRI enhanced with gadolinium liposomes or ultrasmall superparamagnetic iron oxide particles. Study Type...
Article
The development of probes for biomolecular imaging and diagnostics is a very active research area. Among the different imaging modalities, optics emerged since it is a noninvasive and cheap imaging technique allowing real time imaging. In vitro, this technique is very useful however in vivo, fluorescence suffers from low signal-to-noise ratio due t...
Poster
Full-text available
The deleterious role of oxidative stress in neuronal but also vascular lesions following ischemic stroke is clearly established. However, so far no antioxidant strategy has shown clinical efficacy. Cerium oxide nanoparticles (CNPs) have interesting antioxidant capacities via scavenging free radicals and mimicking antioxidant enzymes activities. The...
Article
Full-text available
In the context of increasing liver diseases, no contrast agent is currently available in Europe and united states to directly assess the liver function. Sole, neolactosylated human serum albumin is being clinically used in Asia. In order to perform preclinical studies in the context of liver diseases, we conceived a fluorescent lactosylated albumin...
Article
Nucleic acids (NAs) have been considered as promising therapeutic agents for various types of diseases. However, their clinical applications still face many limitations due to their charge, high molecular weight, instability in biological environment and low levels of transfection. To overcome these drawbacks, therapeutic NAs should be carried in a...
Article
AGuIX® are sub-5 nm nanoparticles made of a polysiloxane matrix and gadolinium chelates. This nanoparticle has been recently accepted in clinical trials in association with radiotherapy. This review will summarize the principal preclinical results that have led to first in man administration. No evidence of toxicity has been observed during regulat...
Article
Amphiphilic triblock copolymers (Atri) made of perfluorinated alkyl chain linked to hydrocarbon chain and methoxy-polyethylene glycol of three different molecular weights were synthesized. In vitro evaluation demonstrated that these new compounds were non-cytotoxic. Characterization and interaction of each triblock copolymer with a branched polyami...
Article
Ultrasmall silica nanoparticles (NPs), having hydrodynamic diameters under 10 nm are promising inorganic platforms for imaging and therapeutic applications in medicine. Herein is described a new way for synthesizing such kind of NPs in a one-pot scalable protocol. These NPs bear DOTA (1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid) ligand...
Article
Full-text available
Purpose: The development of theranostic nanocarriers as an innovative therapy against cancer has been improved by targeting properties in order to optimize the drug delivery to safely achieve its desired therapeutic effect. The aim of this paper is to evaluate the magnetic targeting (MT) efficiency of ultra-magnetic liposomes (UML) into CT26 murin...