Nathalie Boulle

Nathalie Boulle
Centre Hospitalier Universitaire de Montpellier | CHU Montpellier · Département de biopathologie cellulaire et tissulaire des tumeurs

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98
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Publications

Publications (98)
Article
The vaginal ecosystem is a key component of women’s health. It also represents an ideal system for ecologists to investigate the consequence of perturbations on species diversity and emerging properties between organisational levels. Here, we study how exposure to different types of menstrual protection products is linked to microbial, immunologica...
Article
Full-text available
Human papillomaviruses (HPVs), the most oncogenic virus known to humans, are often associated with Herpes Simplex Virus-2 (HSV-2) infections. The involvement of the latter in cervical cancer is controversial but its long-term infections might modulate the mucosal microenvironment in a way that favors carcinogenesis. We know little about coinfection...
Preprint
Human papillomaviruses (HPVs), the most oncogenic virus known to humans, are often associated with Herpes Simplex Virus-2 (HSV-2) infections. The involvement of the latter in cervical cancer is controversial but its long-term infections might modulate the mucosal microenvironment in a way that favors carcinogenesis. We know little about coinfection...
Preprint
Full-text available
Objective To determine if the use of menstrual cups rather than tampons is associated with more or less health risk. Design Analysing biological, demographic, and behavioural data in a cohort of women who reported using mostly tampons ( n = 81) or menstrual cups ( n = 22). Setting A cross-sectional analysis using the inclusion data of a single ce...
Article
In France, cervical cancer screening based on cervical smear has a participation rate of around 60%. New screening strategies are encouraged to increase the participation of under-screened women, including vaginal self-sampling with high-risk human papillomavirus (HR-HPV) testing. This study was based on the distribution of an anonymous self-admini...
Article
Understanding genital infections by Human papillomaviruses (HPVs) remains a major public health issue, especially in countries where vaccine uptake is low. We investigate HPV prevalence and antibody status in 150 women (ages 18 to 25) in Montpellier, France. At inclusion and one month later, cervical swabs, blood samples and questionnaires (for dem...
Preprint
Background. Understanding genital infections by Human papillomaviruses (HPVs) remains a major public health issue, especially in countries where vaccine uptake is low. Methods. We investigate HPV prevalence and antibody status in 150 women (ages 18 to 25) in Montpellier, France. At inclusion and one month later, cervical swabs, blood samples and qu...
Article
Full-text available
Introduction Human papillomaviruses (HPVs) are responsible for one-third of all cancers caused by infections. Most HPV studies focus on chronic infections and cancers, and we know little about the early stages of the infection. Our main objective is to better understand the course and natural history of cervical HPV infections in healthy, unvaccin...
Article
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Estrogens play a pivotal role in breast cancer etiology, and endocrine therapy remains the main first line treatment for estrogen receptor alpha (ERα) positive breast cancer. ER are transcription factors whose activity is finely regulated by various regulatory complexes, including histone deacetylases (HDACs). Here, we investigated the role of HDAC...
Article
Full-text available
Infections of stratified epithelia contribute to a large group of common diseases, such as dermatological conditions and sexually transmitted diseases. To investigate how epithelial structure affects infection dynamics, we develop a general ecology-inspired model for stratified epithelia. Our model allows us to simulate infections, explore new hypo...
Data
Effect of re-seeding on wart-associated HPV infection kinetics. a. Infection with baseline parameters. b. Here infection rate of basal cells β decays to zero with time (with decay rate b = 0.05). No wart-like manifestation of the infection is possible. (EPS)
Data
Chlamydia dynamics. a. Time series of uninfected cells (Ud and Up) and the infected cells of the same layers (Id and Ip). Infection with baseline parameters. (EPS)
Data
Supporting information. Supplementary methods and results. (PDF)
Data
Non-stratified HPV infection model schematic. A population of target cells, T, becomes infected by interacting with free virions, V at a rate β. Infected cells, I, self-proliferate, ρa, due to HPV infection. Infected cells die naturally, μ, and release the virions they contain with a burst size of θ. Free virions are cleared by mucus, ζ, and infect...
Data
Supporting code. Mathematica file that generates figures for non-stratified model. (NB)
Data
Supporting code. R file that uses 3 csv data files for model fits. (R)
Data
Effect of parameter variations on the kinetics of HR-HPV infection. a. Infection with baseline parameters. b. and c. HR-HPV gives wart-like infections with either higher HPV-driven proliferation, (3x αb) or higher burst size (1 order of magnitude higher), thus demonstrating that HR-HPVs need to keep both of these parameters down in order to have fl...
Data
Non-stratified HPV model. A. Time series of i. low cell proliferation driven by HPV infection (wart-associated-HPV-like), ρa = 0.7 and θ = 106, and ii. high cell proliferation by HPV infection (HR-HPV-like), ρa = 1.4 and θ = 104. Cell accumulation and duration are the opposite of what is seen in real infections, i.e. HR-HPV infections should accumu...
Article
Background: With population ageing, post-menopausal women represent a new group to be considered in cervical cancer screening strategies, including the significance of High Risk (HR)-HPV detection. Objectives: A retrospective analysis was conducted in a cohort of 406 menopausal women attending routine gynaecological consultation at the Hospital...
Article
Background: Little is known about human papillomavirus (HPV) shedding in human breast milk. Objective: To investigate HPV shedding in mature breast milk specimens collected from breastfeeding African women living with HIV-1 and not receiving antiretroviral treatment. Design: 62 African women enrolled in the ANRS 12174 trial participated in thi...
Preprint
Full-text available
Infections of stratified epithelia collectively represent a large burden on global health. Experimental models provide a means to understand how the cell dynamics themselves influence the outcomes of these infections. Mathematical approaches are needed to improve quantification and theoretical advancement of these complex systems. Here, we develop...
Article
A 17-year-old girl displayed symptoms of intra-cranial hypertension revealed by chronic diffuse cephalalgia. She underwent surgery for strabismus in childhood and had clinical evidence of ocular albinism (OA), characterized by a partially transilluminated iris at eye examination, and a heterogeneous retinal pigmentation at fundoscopy. This conditio...
Article
Objectives: To investigate the presence of recently discovered human polyomaviruses in cervical specimens collected from African and French women, in relation to HIV serostatus, high-risk human papillomavirus infection (HR-HPV) and cervical disease. Methods: Cervical specimens were collected from 140 HIV-1-seropositive African women and 50 HIV-s...
Article
Full-text available
Histone lysine acetylation is an epigenetic mark regulated by histone acetyltransferases and histone deacetylases (HDAC) which plays an important role in tumorigenesis. In this study, we observed a strong overexpression of class IIa HDAC9, at the mRNA and protein levels, in the most aggressive human breast cancer cell lines (i.e. in basal breast ca...
Article
Full-text available
To investigate the presence of recently discovered human polyomaviruses in cervical specimens collected from African and French women, in relation to HIV serostatus, high-risk human papillomavirus infection (HR-HPV) and cervical disease. Cervical specimens were collected from 140 HIV-1-seropositive African women and 50 HIV-seronegative French wom...
Article
Reactive oxygen species (ROS) are key players in the apoptotic effects induced by short-term tamoxifen treatment of breast cancer cells, but also in acquired resistance following long-term treatment. Whereas the use of the selective estrogen receptor down-regulator fulvestrant is promising, especially in patients who develop tamoxifen resistance, o...
Article
Full-text available
The inhibitors of mutant BRAF that are used to treat metastatic melanoma induce squamoproliferative lesions. We conducted a prospective histopathological and molecular study on 27 skin lesions from 12 patients treated with vemurafenib. Mutation hot spots in HRAS, NRAS, KRAS, BRAF, and Pi3KCA were screened. HPV and HPyV infection status were also de...
Article
But de l’étude L’implication du virus Human Papillomavirus (HPV) dans les papillomes naso-sinusiens reste débattue. Selon les études, environ un tiers des papillomes renferme de l’HPV. Le rôle de l’HPV dans la transformation maligne demeure également discuté. Dans une série de 35 patients nous avons étudié la corrélation entre la présence d’HPV et...
Article
La présence de polyomavirus humains dans le tractus génital féminin a été très peu explorée jusqu’ici. Quelques rares études ont toutefois rapporté l’association de certains polyomavirus avec des lésions cervicales précancéreuses ou cancéreuses. Nous avons recherché la présence de « nouveaux » polyomavirus (MCPyV, HPyV6, HPyV7 et TSPyV) dans les pr...
Article
La prévalence de l’infection HPV dans la population féminine varie avec l’âge, avec un pic d’environ 30 % autour de 20–25 ans. Elle diminue ensuite progressivement après l’âge de 30 ans pour devenir inférieure à 10 %. Plusieurs études ont cependant rapporté une ré-augmentation de la prévalence de l’infection HPV pouvant atteindre jusqu’à 15 % à par...
Article
In France, cervical screening is opportunistic and approximately 40% of women do not attend regular screening programs. The aim of this study was (1) to assess the prevalence of human papillomavirus (HPV) cervical infection and of cytological abnormalities in a population of young pregnant women with poor adherence to cervical cancer screening and...
Article
Full-text available
Animal models of atherosclerosis suggest that B cells have contradictory protective or proatherogenic effects that are also subset and context dependent. To further understand the pathophysiology of human atheroma, we characterized local Ig production and functional properties of resident B cells in human arterial lesions. Ig repertoires were analy...
Poster
Ig repertoires were analyzed by RT-PCR on tissue extracts from carotid endarterectomy samples of 13 atherosclerotic patients. Cytokine, surface marker and transcription factor expression was studied on adventitial lymphocytes isolated by laser capture microdissection (LCM). In adventitia, B lymphocytes constituted diffuse infiltrates or formed smal...
Article
Full-text available
Formalin is the key agent for tissue fixation and pathological diagnosis. However, it poorly preserves nucleic acids and this can impair molecular studies. An alternative to formalin would be a fixative which can allow both morphologic and molecular analyses. To assess the suitability of such a fixative, breast (n = 11) and colon (n = 12) tumor sam...
Article
Background: Estrogen receptors (ER) drive breast cancer development through the regulation of gene transcription and the inhibition of ER signalling is the main therapeutic strategy for hormone-dependent breast cancers. Selective ER modulators (such as tamoxifen) or aromatase inhibitors are commonly administered to patients. However, de novo and ac...
Poster
Estrogens play a pivotal role in the etiology of breast cancer. Estrogen receptor (ER) activity is finely regulated by various transcriptional regulatory complexes including histone acetyl transferases (HAT) and histone deacetylases (HDACs). Previous studies have underlined the potential role of some HDACs in breast tumor progression and their inte...
Article
Full-text available
The estrogen receptor (ER) regulates transcription of specific genes by interacting with multiprotein complexes containing his tone acetyltransferase (HAT) and histone deacetylase (HDAC). RE and their transcriptional core gulators can be modified by acetylation on lysine residues. This post-translational modification controls their ability to inter...
Article
The PapilloCheck® assay was compared with the Digene HC2 HPV DNA assay for the detection of 13 high-risk human papillomaviruses (HPV) in 240 samples, including 181 cervical scrapes and 59 anal scrapes. Overall, 75 (30.5%) samples were positive by the Digene HC2 HPV DNA assay: 34 (18.8%) cervical scrapes and 41 (69.5%) anal scrapes. By considering o...
Article
Molecular analysis of tissue lesions is increasingly used in laboratories to identify new prognostic and therapeutic markers. Formalin has long been the tissue fixative of choice in the laboratories of pathology, as it preserves tissue morphology allowing accurate histological diagnosis. However, formalin is highly toxic and alters nucleic acids an...
Article
Full-text available
Estrogens play an essential role in the normal physiology of the breast as well as in mammary tumorigenesis. Their effects are mediated by two nuclear estrogen receptors, ERα and β, which regulate transcription of specific genes by interacting with multiprotein complexes, including histone deacetylases (HDACs). During the past few years, HDACs have...
Article
Human papillomavirus (HPV) detection and typing using the PapilloCheck test and cytological examination were carried out in anal samples collected from 67 men seropositive for human immunodeficiency virus (HIV) who have sex with men. Fifty (74.6%) patients had anal HPV infection, 46 (68.7%) had high-risk (HR) HPV infection, and 38 (56.7%) had multi...
Article
Full-text available
Molecular diagnosis has been proposed to enhance the intra-operative diagnosis of sentinel lymph node (SLN) invasion in head and neck squamous cell carcinoma (HNSCC). Although cytokeratin (CK) mRNA quantification with real-time reverse transcriptase-PCR (QRT-PCR) has produced encouraging results, the more discriminating markers remain to be identif...
Article
Over the past few years there has been a considerable development of DNA amplification by polymerase chain reaction (PCR), and real-time PCR has now superseded conventional PCR techniques in many areas, e.g., the quantification of nucleic acids and genotyping. This new approach is based on the detection and quantification of a fluorescent signal pr...
Article
Introduction L’importance croissante de l’évaluation des anomalies génétiques dans le diagnostic et la prise en charge thérapeutique des cancers colorectaux, associée à une reconnaissance du caractère cancérigène du formol engage de nombreux laboratoires de pathologie dans une démarche de substitution du formol. A ce titre, le nouveau fixateur RCL2...
Article
Full-text available
Although numerous studies have underlined the role of histone deacetylases (HDAC) in breast physiology and tumorigenesis, little is known on the particular contribution of the various classes of HDACs in these processes. Using estrogen receptor-alpha (ERalpha)-positive MCF-7 breast cancer cells, the effects of MC1575 and MC1568, two novel class II-...
Article
Thymidine phosphorylase (TP)/platelet-derived endothelial cell growth factor is associated with tumor angiogenesis. We evaluated the TP mRNA and protein expression in basal cell carcinomas (BCC) and in various skin tumors including numerous BCC histological simulants. Immunohistochemistry was performed on 99 paraffin sections of formalin-fixed skin...
Article
Full-text available
In mammalian cells, the level of estrogen receptor alpha (ERalpha) is rapidly decreased upon estrogen treatment, and this regulation involves proteasome degradation. Using different approaches, we showed that the Mdm2 oncogenic ubiquitin-ligase directly interacts with ERalpha in a ternary complex with p53 and is involved in the regulation of ERalph...
Article
L’acquisition par les cellules cancéreuses d’un phénotype métastatique représente un des aspects les plus péjoratifs de la progression tumorale. Ce phénotype se caractérise par l’invasion des tissus voisins, par la migration des cellules métastatiques via le sang et la lymphe, vers des organes qu’elles sont capables d’envahir et dans lesquels elles...
Article
Heterotrimeric G proteins are signal transduction proteins coupled to hormone receptors that activate intracellular second messenger systems, mainly cyclic adenosine monophosphate mediated protein kinase. Recent studies indicate that G proteins may have a major role in oncogenesis as well as in tumor invasiveness and cell proliferation. The involve...
Article
A key challenge in the clinical proteomics of cancer is the identification of biomarkers that would enable early detection, diagnosis and monitoring of disease progression to improve long-term survival of patients. Recent advances in proteomic instrumentation and computational methodologies offer a unique chance to rapidly identify these new candid...
Article
Methacarn and RCL2, a new noncrosslinking fixative, were compared to formalin-fixed or frozen tissue samples of the same invasive breast carcinoma and were evaluated for their effects on tissue morphology and immunohistochemistry as well as DNA and RNA integrity. The histomorphology of methacarn- or RCL2-fixed paraffin-embedded tumors was similar t...
Article
Full-text available
The main goal of sentinel lymph node (SLN) detection in head and neck squamous cell carcinomas is to limit neck dissections to pN+ cases only. However, intraoperative + diagnosis cannot be routinely done using the current gold standard, serial step sectioning with immunohistochemistry. Real-time quantitative reverse transcription-PCR (RT-PCR) is po...
Article
The proliferative action of ERalpha largely accounts for the carcinogenic activity of estrogens. By contrast, recent data show that ERbeta displays tumor-suppressor properties, thus supporting the interest to identify compounds that could increase its activity. Here, we show that histone deacetylase inhibitors (HDI) upregulated ERbeta protein level...
Article
Full-text available
The proliferative action of ERα largely accounts for the carcinogenic activity of estrogens. By contrast, recent data show that ERβ displays tumor-suppressor properties, thus supporting the interest to identify compounds that could increase its activity. Here, we show that histone deacetylase inhibitors (HDI) upregulated ERβ protein levels, whereas...