Nataliya Ashukina

Nataliya Ashukina
National Academy of Medical Sciences of Ukraine · Sytenko Institute of Spine and Joint Pathology

PhD, Candidate of Biol. Sci

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75
Publications
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66
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Publications

Publications (75)
Article
Full-text available
Every year, more and more people suffer from illnesses and disabilities that occur due to lumbar pain. Many studies, some of that use in-vivo models, are conducted to decrease the socioeconomic impact of the consequences of degenerative spine diseases. Objective. To evaluate the advantages and disadvantages of different in vivo models that are use...
Article
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Objective: The aim: To assess the bone remodeling around highly porous titanium cups TTM in patients with low bone mass one year after total hip arthroplasty (THA). Patients and methods: Materials and methods: In this prospective study were included 18 patients (18 hips), whom was performed primary THA with the use of TTM cups. According to T-sc...
Article
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Bone tissue engineering strategy involves the 3D scaffolds and appropriate cell types promoting the replacement of the damaged area. In this work, we aimed to develop a fast and reliable clinically relevant protocol for engineering viable bone grafts, using cryopreserved adipose tissue‐derived mesenchymal stromal cells (MSCs) and composite 3D colla...
Article
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The number of elderly people is constantly increasing all over the world. They are most often the patients who need orthopedic surgeries like arthroplasty, osteosynthesis and others. It is known that the process of bone regeneration depends on the patient's age. However, certain characteristics of bone regeneration process depend on the age remain...
Article
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Background. The reconstruction of long bone defects that occur after resection of tumors is a problem that requires constant study. Bone allografts are often used in this scenario. Unfortunately, while they are prepared, allografts partially lose their strength and osteoinductive properties; their survivability in oncological patients is only 40% a...
Article
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Based on the systematic approach to the diagnosis of spinal pathology, there are no fundamental differences in the etiology of spine degenerative disease, facet joints arthritis, and other diseases. These diseases are considered multifactorial: age (aging), systemic regulatory factors (hormones, peptides, cytokines), genetic predisposition, inadequ...
Article
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The objective of the study is to determine the current tendencies in the use of osteoplastic materials based on tricalcium phosphate (TCP) in orthopedics and traumatology. Methods. The search of the scientific information for the analysis was carried out in the PubMed, Google Scholar, World Digital Library, ScienceDirect. Results. The development o...
Article
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Rheumatoid arthritis is an inflammatory autoimmune disease that occurs as a result of impaired immune tolerance, leading to an aberrant immune response to autologous antigens. Multipotent mesenchymal stromal cells (MMSCs) and the biologically active substances they produce can promote the activation of regenerative processes in the organism not onl...
Article
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Aim: To analyze the influence of heat stable anti-A and anti-B antibodies of the persons with anti-A and anti-B absorbing ability of erythrocytes different from their blood group type on erythrocyte membrane. Materials and methods: Reaction of hemagglutination with complement at room temperature, 4°C, 37°C with the heated sera, elution and method...
Article
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The aim of the study is to improve the method of surgical treatment for patients with tumors of long bones with the use of segmental bone allograft. Material and method. The authors have improved the method of surgical treatment of patients with tumor bone lesions using segmental allografts. Step-cut osteotomy was performed to achieve the best con...
Article
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Objective. To study internal tensions in bone and soft tissues of shin at normal condition and at isolated tibia nonunion, the range of fragments displacement, callous structure and to sustain the conception of treatment. Methods. Due to finite-element method we created three models: I-anatomic norm; II-transverse defect of 5 mm height on the middl...
Poster
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Background According to independent multicenter studies the tibia shaft nonunion is about 12%. Earlier, A.Sarmiento, L. Latta (1981) proposed to perform resection of a fragment of the fibula at the functional treatment of tibia nonunion.
Article
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Prospective trends in the development of glass-ceramic materials for bone endoprosthesis replacement were analyzed. The glass-ceramic materials based on calcium phosphate silicate glasses containing modifying components (MgO, CoO, V2O5, SrO, Cu2O, MoO3 and La2O3) were suggested. The period of resorption of the developed glass-ceramic materials was...
Article
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Objective: To investigate the relationship between the structure of articular cartilage and subchondral bone of the knee or hip joint in postmenopausal women with osteoarthrosis.Methods: The postoperative material was obtained during the operations of total hip or knee arthroplasty of 20 women with X-ray confirmed knee (n=10) or hip (n=10) osteoart...
Article
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Background To compare structural features of the femoral bone of ovariectomized and non-ovariectomized rats after implantation of porous materials (TANTALUM, CONCELOC, TTM, ATLANT). Methods Experiments were carried out on 56 white laboratory female rats aged 6 months. Rats were randomly assigned into groups: sham-operated control group (SH) or ova...
Preprint
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Background To compare structural features of femoral bone of ovariectomized and non-ovariectomized rats after implantation of porous materials (TANTALUM, CONCELOC, TTM, ATLANT). Methods Experiments were carried out on 56 white laboratory female rats aged 6-months. Rats were randomly assigned into groups: Sham control group (SH) or ovariectomy group...
Preprint
Full-text available
Background: To compare structural features of femoral bone of ovariectomized and non-ovariectomized rats after implantation of porous materials (TANTALUM, CONCELOC, TTM, ATLANT). Methods: Experiments were carried out on 56 white laboratory female rats aged 6-months. Rats were randomly assigned into groups: Sham-operated control group (SH) or ovarie...
Article
It is possible that testicular dysfunctions, including idiopathic infertility in men, may be caused by changesin connective tissue, which are characterized by disorders of its metabolism, the content of collagen and glycosaminoglycans(GAGs). Such patients quite often have the anomalies of the genital system. It is the anomaliesin the development of...
Article
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This study investigated a person with an AB0 discrepancy. Her blood group initially typed at the birth as AB Rh+ (positive); however, it was B Rh+ (positive) or Rh- (negative) when she was in her teens. At room temperature, her erythrocytes were agglutinated by anti-B, and the agglutination was significantly weaker at 37 ºC. As a result, her erythr...
Article
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The aim is to study the features of the influence of local ultraviolet radiation on the organization and structure of collagen fibers in the radiation area. The studies were performed on 30 albino guinea pigs weighing 400-500 g, exposed to local ultraviolet radiation. Animals were removed from the experiment after 2 hours, 4 hours, 3 days, 8 days a...
Article
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The difficulties of the organ-preserving operations on the proximal tibia are associated with the need to cut off the patellar ligament during surgical access for ablastic tumor resection, which causes difficulties in restoring the function of the knee joint in the postoperative period. Objective: on experimental rats to determine the morphological...
Article
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The aim is to study the bone response to the implantation of two glass-ceramic materials into the distal metaphysis of the femur of rats. Experiment was carried out on 30 white 4-month-old laboratory male rats. Animals were randomly divided into two groups for the implantation of two types of calcium silicate-phosphate glasses (BS-11 or ASZ-5) into...
Article
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Blocking of the growth plate (GP) using plates with screws (tension band plating) is a modern method used to correct deformities and moderate leg length discrepancy in growing children. Determining the duration of temporary bilateral blocking without the occurrence of irreversible changes of GP is of paramount importance important. Methods: Two-mon...
Article
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Objective. To analyze the methods of revealing weak A and B antigens on the erythrocytes in AB0 system. Methods. Patients after knee and joint arthroplasty were examined on group-specific characteristics with revealing weak A and B antigens on the erythrocytes. Methods of absorption, agglutination, agglutination with complement and antiglobulin tes...
Article
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The aim is to study the characteristics of bone regeneration under the conditions of implantation of carbon/carbon composite material with and without pyrocarbon coating in the vertebral bodies of rat. C/c composite (n=9) or c/c composite & PC (n=9) were implanted into hole defects in the LIII vertebral bodies of white laboratory male rats (6-7 mon...
Patent
СПОСІБ МОДЕЛЮВАННЯ СПОСОБУ ФІКСАЦІЇ ІМПЛАНТОВАНОГО АЛОКОМПОЗИТНОГО ЕНДОПРОТЕЗА ПРОКСИМАЛЬНОГО ВІДДІЛУ СТЕГНОВОЇ КІСТКИ Реферат: Спосіб моделювання способу фіксації імплантованого алокомпозитного ендопротеза проксимального відділу стегнової кістки, заснований на моделюванні дефекту проксимального відділу стегнової кістки, здійснюють шляхом проведенн...
Article
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Adamantinoma is one of the rare bone tumors. It demonstrates a large variety of morphological patterns and specific radiological data. The complexity of the diagnosis and the rare frequency of this disease are the main reasons for difficult diagnostic verification and patients treatment. The profile of the adamantinoma of the distal tibia, T2N0M0,...
Article
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In vivo research is an important link in the development of osteoplastic materials. Aim: to study the structural features and markers of bone metabolism after an implantation into the distal metaphysis of the rat femur of glass crystalline calcium phosphate material (GCM). Methods: hole defects in the distal metaphysis of the femur were performed i...
Article
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In the elderly and senile age, osteoporosis and spinal osteochondrosis are found with high frequency, which determines the study of their common development mechanisms. Objective: to study the level of 25(OH)D and 1.25(OH)2D3 and indicators of bone metabolism in the blood serum of postmenopausal women with spinal osteochondrosis and low bone minera...
Article
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Finding the optimal graft for filling bone defects and optimizing reparative osteogenesis are actual problems of orthopаedics and traumatology. The peculiarities of the restructuring and the biocompatibility of plastic materials saturated with bioactive factors of blood serum are poorly understood. Objective: to study the morphological features of...
Article
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Achilles tenopathy is one of the common diseases of the lower limb in sportsmen. Prevalence of achilles tendopathy is 60 % out of all such pathologies occurring in the foot area. In most cases, the treatment of this condition is conservative, using the methods of physical rehabilitation, as well as non-steroidal anti-inflammatory drugs. Objective:...
Article
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Today, the technology of open reposition and external osteosynthesis (AO) is leading one in the treatment of intraarticular fractures. In the presence of many positive characteristic of these technologies, their high invasiveness, causing a significant amount of early infection necrotic complications and late regeneration disorders, is an objective...
Article
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The prevalence of degenerative changes in the intervertebral disc and associated pain causes the development methods of minimally invasive therapy for inimize injuries during surgery and to obtain effective results. For microdiscectomy are used various methods in the case of intervertebral disc herniation. However, changes occur at the tissue leve...
Article
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This study focuses on mechanisms which regulate the process of fracture healing. We studied the form and position of the fibrin-blood clots (FBC) in the zone near the fragments among patients with limb fractures: macroscopically (during open surgical operations to reposition bone fragments) and using sonography. We conducted a histological and immu...
Article
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Aim: to investigate the structural and functional changes in paravertebral muscles of patients with degenerative diseases of the lumbar spine based on histological and electron microscopic analyzes. Material and methods: histological and electron microscopic analysis of multifidus muscles of 49 patients (27 men and 22 women) was performed. Patients...
Article
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For the treatment of the moderate leg length discrepancy (2–6 cm) in children surgeons use temporal growth plate (GP or epiphyseal cartilage) block with plates and screws. Objective: to study morphological changes in distal femur growth plate of rabbits under bilateral temporal block with non-locking plates and screws. Methods: we blocked distal GP...
Article
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In the case of instrumental spine fusion with the achieved biomechanical rigidity of the operated segment, the use of platelet-rich fibrin to stimulate osteogenesis is promising. Objective: to study the possibility of lumbar spine fusion under conditions of platelet-rich fibrin usage in the experiment. Methods: fusion modeling was made in 42 mature...
Article
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Background. An increase in the frequency of useful fusion requires new biomaterials that increase the graft osteogenic potential. The purpose of our work was to study the bone fusion formation in experimental posterolateral spinal fusion with the use of bone allograft and local autograft and autologous platelet-rich fibrin. Materials and methods....
Article
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For understanding of reasons of bone nonunion after fractures we have to study the mechanisms which are on the base of cells differentiation. Objective: on the base of clinical and morphologic examination we studied the mechanisms of cells differentiation at regeneration process after shaft fractures. Methods: for histological study we took fibrin-...
Article
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Disturbances of structure and function of paravertebral muscles are one of the risk factors of lumbar spine degenerative diseases. It is proved that muscles play an important role in spine fusion and in other bones fusion. Objective: to analyze the relationship between the results of the posterolateral lumbar fusion after stabilization of LIV–LV ve...
Article
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On the base of literature review the possibility of hyaluronic acid application in the treatment of patients with arthritis and diseases of joint adjacent tissues was assessed. hyaluronic acid is important component of joint tissues, provides its normal function. At arthritis changes appear normal concentration of hyaluronic acid and its molecular...
Article
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In the process of developing methods for treating fractures of vertebral bodies, in particular in case of traumatic injury, it is necessary to understand the biology of the processes of formation and reorganization of the regenerate for the possibility of optimizing reparative osteogenesis. Objective: to assess the effect of platelet rich fibrin on...
Article
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Objective: to study, using immunohistochemical methods, the content of the vascular endothelial growth factor and trans­forming growth factor (TGF-β) in tissues adjacent to bone frag­ments after fracture in humans, as well as in autofibrin obtained in vitro. Methods: biopsy samples (interfragmental fibrin-blood clot, periosteum, muscular and fatty...
Conference Paper
Background. The first stage of fracture healing is considered to be inflammation, which is initiated by tissues ruptures and hematoma formation. In our report we want to perform other point of view. Objectives. To determine the structural peculiarities of fibrin clots in the interfragments space and to study reparation processing at the first two w...
Article
Full-text available
Objective: to assess the structural and functional state of the rat knee after modeling posttraumatic extraarticular deformation of the femur. Methods: experiment was performed in 39 white mature (6 months old) male rats. To model the deformation of varus (test group, 15 rats) after transverse osteotomy of the fe­mur, a bent angled rod was introduc...
Article
Full-text available
The formation of extra-articular traumatic deformities is a com­plication that occurs in about a third of cases with nonunion or pseudarthrosis after isolated diaphyseal fractures of long bones. Objective: based on the analysis of scientific informa­tion to determine the structural and functional properties of ar­ticular cartilage (AC) under physio...
Article
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Methods of fixation are used for the treatment of diaphyseal fractures, which produce structure «fragments – fixation device» with various mechanical properties and the displacement range of fragments. Purpose: to justify the concept of the effect of the displacement of bone fragments in the process of formation of the regenerate in the treatment o...
Article
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Structural violations in paravertebral muscles — spinal units stabilizators, are related to the risk factors for degenerative lumbar spane diseases. Experts pay attention to their condition after the surgical treatment — spinal fusion. However effect of muscle activity on the result of surgical treatment is not studied well. Subject: to evaluate re...
Article
Full-text available
Stable posttraumatic deformities of long bones lead to changes in interrelationships in damaged segments that could cause dysfunction of muscles and ligaments, joint surface integrity and range of motion in limb joints. The goal: to investigate in experiment on rats the structural peculiarities of muscles adjacent to peak of posttraumatic femur dia...
Article
Full-text available
Multifidus muscles located in the middle layer of paravertebral muscles provide support for the positioning of the spine, its rota­tion and extension movements. Changing in their structure with age, resulting in injuries or degenerative processes lead to their dysfunction. Objective: To investigate the structural features of the multifidus muscle (...
Article
Full-text available
Bone regeneration is a cascade of cellular and molecular phe­nomena leading in the fracture region to the formation of bone tissue which is identical to the original one. The process is divided into stages between which it is impossible to draw a clear line. Objective: To identify key clinical and morphological phenomena that define the staging pro...
Article
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Based on the analysis of scientific literature authors summarized information about the structural organization of the paravertebral muscles in normal condition and degenerative diseases of the lumbar spine. It is proved that in the paravertebral muscles originating from the dorsal myotomes (m. multifidus, m. erector spinae) unlike other skeletal m...
Article
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Despite on significant progress in the development of vertebrology the problem of treatment of degenerative diseases of the spine remains relevant due to the significant amount of people suffering from back pain. One of the approaches to the study of the etiopathogenesis of degenerative diseases of the spine might be conducting experimental studies...
Article
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According to the Kharkiv interregional MSEC occurrences of nonunion fractures requiring additional surgical treatment ranges from 12.5 to 26 %. Treatment of such complication appeared requires weighed approach which might take into consideration its causes and clinical features in order to repeated surgery to be the last one. Purpose: to evaluate c...
Article
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The authors examined venous blood of three volunteers and determined the time of the formation (45-60 minutes) of a fibrin-blood clot in closed space in vitro, as well as they studied the effects of mechanical factors (pressure and mixing) on the rate of this formation. Dependence of the structure and mechanical properties of the formed fibrin upon...
Article
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An experimental study has been carried out on 64 white laboratory female rats aged 6 months. It has been established that a decrease of the thyroid hormone level in the animals by introduction of Thiamazol (1 mg/100 g body weight, per os) causes a delay of the repair process in the femur diaphysealdefect for all the periods of the research. Abnorma...
Article
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The experiment was carried out on 75 white rats (age of 6-8 months). During morphological analysis it was determined that continuous injection of high doses of hydrocortisone (5 mg/kg body weight) to mature rats inhibits osteoreparation process in femur metaphyseal defects. Introduction of L-thyroxine (25 μg/100 g body weight) causes accelerate for...
Article
Full-text available
Experimental study was carried out on 50 white laboratory female rats (age 6-8 months). It was determined that decrease of thyroid hormone in the animals’ organism by introduce thiamazol (1 mg/100 g body weight, per os) causes the delay of the repair process in the femur metaphyseal defect for all the periods of the research. At the inflammation st...
Article
Full-text available
The assessment of bone mineral density (BMD) was performed at the lumbar spine of practically healthy 447 women at the age from 20 to 80. Dual-energy X-ray absorptiometry was used. The minimal BMD at the vertebral body L I as compared to vertebral bodies L II-L IV was established. BMD was progressive increased in the caudal line. Highest BMD indexe...
Article
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The purpose of this analytical literature review was to generalize results of researches in studying the mineral bone tissue density (MBTD) of the vertebral bodies, adjacent to an area of spondylodesis. It was revealed that spondylodesis was followed by both increases and decreases of MBTD both in the very area of spondylodesis and in adjacent vert...
Article
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У статті наведено результати експериментальних досліджень та клінічного використання різновидів кальцій-фосфатної кераміки для заміщення порожнинних кісткових дефектів кульшової западини у випадках первинного та ревізійного ендопротезування кульшового суглоба. Отримані результати свідчать про ефективність використання кожного із запропонованих виді...
Article
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The article contains data of a histological study of menisci, which were removed because of their destruction in 17 patients during surgical interventions on the knee joints for structure anomalies. Tissues of the removed menisci revealed destructive changes (disturbances in histoarchitectonics, cell necrobiosis, destructions in the collagen fibre...
Article
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Study was carried out at 40 female white laboratory rats (age 6-8 moths). It was determined that excess of thyroid hormone in the organism of rats by introduce L-thyroxine (25 µg/100 g body weight, per os) causes accelerated formation of tissue-specific structures at early stages of repair of bone defect. But in the bone regenerate destruction of c...
Article
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Метою роботи було створення експериментальних моделей післятравматичних діафізарних деформацій стегнової кістки лабораторних щурів. Автори розробили та детально описали моделі з використанням внутрішньокісткового (стабільний та нестабільний остеосинтез) та зовнішнього спице-стрижневого (стабільний остеосинтез) фіксаторів. Розроблені експериментальн...
Article
Full-text available
Experimental modeling of posttraumatic varus deformity of rat femur using intramedullary nailing was conducted. After 1, 3, and 6 months the reparative osteogenesis delay was established during histological analysis. Osteogenesis went according to secondary bone union After 3 months into group of animals having femur deform-ity the destructive fiss...
Article
Full-text available
Analysis of 49 literature sources was carried out. It was shown that thyroid hormones effect on the structural and metabolic characteristics of the bone by direct (through the nuclear receptors) and indirect (due to disbalance of other hormones and biological active substances) ways. Released in the childhood the thyroid hormone excess promotes inc...
Article
Two kinds of calcium phosphate ceramic (CPC) granules of high porosity (50 +/- 5%) and improved (for such materials) compressive strength (10-25 MPa) consisted of hydroxyapatite (PHA) and a mixture of hydroxyapatite (HA) and beta-tricalcium phosphate (beta-TCP) in 60 HA/40 beta-TCP composition (PCPC) were developed. A comparative study of in vivo b...
Article
The experiments were made on 24 rabbits at the age of 2 month. A defect was modelled in the lateral region of the distalmetaphysis and greater trochanter of the femur. The choise of the implantation areas depended upon different conditions of physiological loading. The bone fragments togeser with the implantation areas were examined with help of hi...