Nataliya Archipowa

Universität Regensburg | UR · Institute of Biophysics and physical Biochemistry

The determination of ligand–receptor binding affinities plays a key role in the development process of pharmaceuticals. While the classical radiochemical binding assay uses radioligands, fluorescence-based binding assays require fluorescent probes. Usually, radio- and fluorescence-labeled ligands are dissimilar in terms of structure and bioactivity...

The syntheses of two novel, organic, and chiral photocatalysts are presented. By combining donor-acceptor cyanoarene-based photocatalysts with a chiral phosphoric acid, bifunctional catalysts have been designed. In preliminary proof-of-concept reactions, their use in both enantioselective energy transfer and photoredox catalysis is demonstrated.

Deazaflavins are well suited for reductive chemistry acting via a consecutive photo‐induced electron transfer, in which their triplet state and semiquinone – the latter is formed from the former after electron transfer from a sacrificial electron donor – are key intermediates. Guided by mechanistic investigations aiming to increase intersystem cros...

The Cover Feature shows the photocatalytic oxidative cycloelimination of a coumarin dimer by a flavinium salt inspired by the light‐induced photolyase DNA repair reaction in nature. Light absorption and subsequent intersystem crossing (isc) enable diffusion‐controlled electron transfer (eT) from the coumarin dimer to the excited triplet flavinium s...

Flavinium salts are frequently used in organocatalysis but their application in photoredox catalysis has not been systematically investigated to date. We synthesized a series of 5‐ethyl‐1,3‐dimethylalloxazinium salts with different substituents in the positions 7 and 8 and investigated their application in light‐dependent oxidative cycloelimination...

Drosophila melanogaster cryptochrome functions as the primary blue-light receptor that mediates circadian photo entrainment. Absorption of a photon leads to reduction of the protein-bound FAD via consecutive electron transfer along a conserved tryptophan tetrad resembling the signalling state required for conformational changes and induction of sub...

Hydride transfers play a crucial role in a wide range of biological systems. However, its mode of action - concerted or stepwise - is still under debate. Light-dependent NADPH: protochlorophyllide oxidoreductase (POR) catalyzes the stereospecific trans addition of a hydride anion and a proton across the C17-C18 double bond of protochlorophyllide. W...

Hydride transfers play a crucial role in a wide range of biological systems. However, its mode of action - concerted or stepwise - is still under debate. Light-dependent NADPH: protochlorophyllide oxidoreductase (POR) catalyzes the stereospecific trans addition of a hydride anion and a proton across the C17-C18 double bond of protochlorophyllide. W...

All cryptochromes are currently classified as flavoproteins. In animals their best-described role is as components of the circadian clock. This circadian function is variable, and can be either light-dependent or -independent; the molecular origin of this difference is unknown. Type I animal cryptochromes are photoreceptors that entrain an organism...

The enzyme protochlorophyllide oxidoreductase (POR, EC 1.3.1.33) has a key role in plant development. It catalyzes one of the later steps in chlorophyll synthesis, the light-induced reduction of protochlorophyllide (PChlide) into chlorophyllide (Chlide) in the presence of NADPH. Two isozymes of plant POR, POR A and POR B from barley, which differ i...