Nataliia Kutsokon

Institute of Cell Biology and Genetic Engineering

In vitro shoot regeneration is a fast and reliable method for propagation of valuable tree genotypes and important step in genetic manipulations. This study aimed to establish an optimal in vitro culture initiation and regeneration protocol for highly productive poplar clones ‘Novoberlinska-3’ (Populus pyramidalis × P. laurifolia) and ‘Volosystopli...

Water deficiency is one of the most important abiotic factors limiting the growth and productivity of plants. Under conditions of water deficiency, plants can synthesize osmoprotectors, in particular proline. The aim of our work was to estimate the accumulation of free proline in the leaves of two poplar clones 'Slava Ukrainy', 'Guliver' and willow...

The radiobiologist Alper T. in collaboration with Griffith J.S. proposed that some proteins can cause disease after misfolding. In 1997 Prusiner S.B. was awarded the Nobel Prize for the study of the prions contribution in diseased mammals life. For example, it is supposed that the well-known facts about wild animals' mass death (deer and saiga in A...

Poplar is an economically important forest tree used for multiple applications, including bioenergy production. Although genetic engineering allows a quick introduction of desirable traits, simultaneously, it may deliver unintended side effects. Herein, we compared the protein composition in leaves of microclonally propagated fast-growing hybrid cl...

Soil salinization is an important abiotic factor negatively affecting plant growth, development and productivity. Fast-growing poplar and willow trees are important plants for bioenergy production demonstrating varying degrees of adaptation to different habitats. The study of salt resistance in different clones of poplars and willows will reveal ge...

In the face of global climate changes, studies of bud burst and bud set phenology in trees are necessary to determine the duration of the growing season of plants, the optimal planting period, and seasonal works to achieve high productivity. The purpose of our study was to investigate bud burst phenology in different poplar and willow clones, what...

The irrational anthropogenic influence on soils often leads to negative consequences, one of which is salinization. Among various types of salinity, chloride salinity has the greatest negative effect. Accumulatiоn of chlorine ions by plant cells causes disturbances in their metabolism, as a result of cell membrane damage. Such changes have a negati...

Breeding and cultivation of poplar (Populus sp.) and willow (Salix sp.) was practiced by many researchers throughout the 20th century. Mostly theirs interests were focused on the long-term period of plant cultivation (up to 20 years) with using the wood in timber or paper-pulp industry. Cultivation of poplar and willow under short rotations (up to...

http://biosystems-journal.chnu.edu.ua/index.php?journal=BioSystems&page=article&op=view&path%5B%5D=34

Aim. To carry out genetic transformation of poplar Populus nigra x P. deltoides clone Gradizka with the model gene construct pCB002 carrying selective gene of kanamycin resistance and marker gene of β-glucuronidase. Methods. Genetic transformation was performed with the using leaf, stem and petiole poplar explants. Transformants were selected on th...

A search for ecologically convenient technologies for energy production is becoming more important due to constant increase in anthropogenic environmental load. Using the renewable resources for energy producing, particularly wood biomass from short rotation tree plantations, is accompanied by less pollutants emission into atmosphere. The techno­lo...

Мета. Провести генетичну трансформацію тополі Populus nigra x P. deltoides клон Градізька модельною конструкцією pCB002, що містить селективний ген стійкості до антибіотика канаміцину та маркерний ген β-глюкуронідази. Методи. Генетичну трансформацію проводили із використанням листкових, стеблових та черешкових експлантів тополі. Для виявлення транс...

The genus Populus contains some of the most economically important tree species and hybrids in the world. We compared productivity of short and long-rotation poplar plantations using published data from 23 countries to determine if climate, particularly temperature, had any effect on the observed patterns of productivity. We discovered that climate...

The main advantages that could be obtained by poplar plantation production were described in this review. We also described the significance of poplars for industry and for solutions to ecological problems. Taking into consideration the results obtained by genetic engineering methods, we analyzed the trends in the improvement of the Populus phenoty...

The publications containing data on the generation and study of transgenic plants tolerant to various types of abiotic stresses were analyzed. Mechanisms of plant protection against stresses and genes encoding a wide spectrum of compounds that confer the ability to survive under stress conditions, which cause inhibition of development and are even...

Analysis of genotoxicity is of a great importance due to the pollution of the environment by genotoxicants. The most harmful anthropogenic pollutants as heavy metals, surface-active substances, pesticides and radionuclides are mutagens or/and carcinogens. At date many biotests for determination of genotoxicity in our environment are proposed. Plant...

Rogue" cells determined by human geneticists as the cells with many chromosome aberrations. The main enigma of this phenomenon is its levels are unexpected higher in many studies. Frequently, these cells emergence in human are linked with radiation or viruses. "Rogue" cells supposed to be very important in mutagenesis, oncogenesis and even in evolu...

Analysis of genotoxicity with plants assays is of a great importance due to pollution of the wastewater and soil by genotoxicants. The objective of these studies was to determine the approach to risk assessment contaminated with radionuclide different wastewater from 30-km zone of the Chernobyl and Kyiv region using the transgenic lines Arabidopsis...

Using the Tradescantia and Allium-assays, we analyzed the changes induced both on genes and chromosomes, evaluate the genetic damage of radiation pollutants, studied gene mutations in Tradescantia-SH. Both assays used are supposed to be the sensitive biomonitors of the genotoxicity of environmental factors. Our results demonstrate contaminated soil...

The average number of aberrations per aberrant cell was concluded to carry out information on chromosome instability peculiarities induced by different mutagens as it was shown in our previous work. The purpose of the current study was to present comparative analysis of intercellular distribution of number of aberrations and their theoretical appro...

The average number of aberrations per aberrant cell was concluded to carry out information on chromosome instability peculiarities induced by different mutagens as it was shown in our previous work. The purpose of the current study was to present comparative analysis of intercellular distribution of number of aberrations and their theoretical appro...

Analysis of chromosome instability (CI) is of great importance in view of pollution of the environment by genotoxic factors. Frequency of aberrant cells, spectrum of chromosome aberrations, damages of aberrant cell and distribution of aberrations in the cells are the most conventional parameters of CI. We have carried out the comparative analysis o...

The purpose of this study was to elucidate the genome damage induced by 241Am-irradiation using different parameters of cytogenetic evaluation in Allium-test. The root tip cells test-system for the cytogenetic effects studying was used. 241AmCl3 of different concentrations was used (1.5 x 10(-9)-1.5 x 10(-7) g/l). Water solution-to-plant transfer f...