Nataliia Hudz

• Doctor of Pharmaceutical sciences. Professor
• Professor at Danylo Halytsky Lviv National Medical University

Background: Carotenoids are bioactive tetraterpenoid C40 pigments that are actively synthesized by plants, bacteria, and fungi. Compounds such as α-carotene, β-carotene, lycopene, lutein, astaxanthin, β-cryptoxanthin, fucoxanthin, and zeaxanthin have attracted increasing attention for their antiaging properties. They exhibit antioxidant, neuroprote...

In the current economic conditions, an active search is conducted for cost-effective and well-balanced feeds and feed mixtures for fish farming. The development of functional feeds using biomass of wild algae is becoming a central focus in the fish feed production. Although these supplements offer a promising path to sustainable and environmentally...

This study aimed to analyze the compositions of flavonoids and phenolic acids in the aerial parts of two Artemisia L. species (Artemisia ludoviciana Nutt. and Artemisia campestris L.) when grown in Ukraine. High-performance liquid chromatography analysis (HPLC) detected the presence of 11 flavonoids and 10 phenolic acids in the plant materials unde...

The anti-aging effects of alpha-lipoic acid (αLA), a natural antioxidant synthesized in human tissues, have attracted a growing interest in recent years. αLA is a short-chain sulfur-containing fatty acid occurring in the mitochondria of all kinds of eukaryotic cells. Both the oxidized disulfide of αLA and its reduced form (dihydrolipoic acid, DHLA)...

Bee bread is one of the least studied bee products. In this study, ten bee bread samples were characterized using palynology and HS–SPME–GC–MS (headspace solid-phase microextraction gas chromatography-mass spectrometry). In total, over one hundred different volatile components were identified, belonging to different chemical groups. Only ten common...

Aim: To determine the role of mitochondrial dysfunction in the pathogenesis of atherosclerosis based on the analysis of research data and statistics from the MEDLINE, Scopus and Web of Science Core Collection electronic databases for 2007-2023. Materials and Methods: A comprehensive review of literature sources from the MEDLINE, Scopus and Web of S...

Background Propolis and its major phenolic compound, caffeic acid phenethyl ester (CAPE), have garnered considerable scientific interest due to their anti-inflammatory properties and potential therapeutic applications. Objectives This narrative review explores the potential utility of CAPE in cancer treatment. Methods We comprehensively reviewed...

This review aims to analyze Mentha piperita L. as a potential raw material for the development of new health-promoting products (nutraceuticals, cosmetics, and pharmaceutical products). A lot of scientific publications were retrieved from the Scopus, PubMed, and Google Scholar databases which enable the study and generalization of the extraction pr...

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An alcohol hangover is a complex set of adverse symptoms that occur after excessive consumption of alcoholic beverages. Hangovers are not solely caused by ethanol, but mainly by its toxic metabolites, such as acetaldehyde and acetic acid. Most of the negative symptoms occur when the concentration of alcohol in the blood decreases after drinking. Gi...

Satureja montana L. is widely used in traditional medicine and cooking. New varieties of this species are created and grown for different purposes. Essential oil (EsO) samples were obtained from the fresh flowering shoots of four new Ukrainian cultivars of S. montana: 'Krymski smaragd', 'Lunata', '697-1', and 'N3-18'. The EsO of cultivars 'Lunata'...

The study aimed to analyse the adverse drug reactions report form data received by the State Expert Center of the Ministry of Health of Ukraine from healthcare professionals in the Lviv region in 2022. Regarding specific types of medicines, the ones with proven cause-and-effect relationships that caused the highest frequency of adverse drug reactio...

In this article we presented some facets of the development of liquid ethanolic extracts of Salvia sclarea L. The primary purpose was to elaborate the analytical procedure of the quantitative determination of the total flavonoid content with the establishing dominating group of flavonoids in the extracts of Salvia sclarea by colorimetric aluminum c...

The yield of essential oils isolated from plants is not very high and the hydrodistilled residue by-products rich in polyphenols could be used to increase the profitability of such plant raw materials [1–4]. The aim of the study was to evaluate the compositions and contents of phenolic compounds in the dry extracts obtained from the by-product of A...

The physicochemical characteristics of phycocyanin extracted from cyanobacteria collected in Kaunas Lagoon were studied (spectrum characteristics, C-PC content in the dry mass and chemical purity). It was determined that the tested concentrations of C-PC in purified water should be in the range of 0.02–0.16% for measuring C-PC content in the dry ma...

In this study gives the characteristic of Salvia officinalis L. genotypes (Lamiaceae)grown in the Southern Steppe of Ukraine. S. officinalis one of the most well-known plants from Salvia L. genus that is used as an aromatic, medicinal, and culinary herb. The plant raw material of this plant is characterized by numerous biological activities that al...

Dear Colleagues! Essential oils are a complex mixture of plant volatile compounds which are mainly terpenoids or phenolic compounds for their nature. These compounds are synthesized in dedicated cell types present in almost all parts of the plant, from roots and leaves to flowers and fruits. There is a huge difference between chemotypes of the same...

Assessment of the prospects of introduced and created varieties of Lavandula angustifolia L. in the conditions of the Southern Steppe in terms of yield and essential oil content in raw materials, as well as the influence of the weather conditions of the year on these economically valuable traits is given. The study included 11 samples of Lavandula...

Aim. The aim of the study was to develop the methodical approach to determine the antioxidant activity of the tinctures of Monarda fistulosa herb and flowers and roots of Echinacea purpurea, as well as to develop their technology in laboratory conditions. Materials and Methods. The following methods were used: analysis, synthesis, systematization,...

Мета роботи. Провести ідентифікацію поліфенолів та терпеноїдів у траві Thymus pulegioides (сорт «2/6-07»), Thymus richardii (сорт «Fantasy») і Thymus vulgaris (сорт «Jalos») з використанням методу тонкошарової хроматографії (ТШХ), а також здійснити газохроматографічний аналіз ефірної олії Thymus richardii. Матеріали і методи. Метод тонкошарової хро...

ABSTRACT The aim of the work. To perform the identification of phenolic compounds and terpenoids in the aerial parts of Thymus pulegioides L. (cultivar '2/6-07'), Thymus richardii Pers. (cultivar 'Fantasy') and Thymus vulgaris L. (cultivar 'Jalos') using thin layer chromatography (TLC), as well as to conduct the gas-chromatographic analysis of Thym...

Silica nanoparticles were applied as the carrier of chloramphenicol (2,2-dichloro-N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]acetamide), and were loaded in a 1% carbopol-based gel (poly(acrylic acid)), which allowed obtainment of an upgraded drug form. The samples of silica materials were obtained by means of modified Stöber synthesis, a...

Cyano-phycocyanin is one of the active pigments of the blue-green algae and is usually isolated from the filamentous cyanobacteria Arthrospira platensis Gomont (Spirulina). Due to its multiple physiological functions and non-toxicity, cyano-phycocyanin may be a potential substance for the topical treatment of various skin diseases. Considering that...

New cultivars of lavender adapted to arid steppe conditions were developed by the Institute of Rice of Ukrainian National Academy of Agrarian Sciences (NAAS). This work is a part of the characterization process of the new cultivars. The chemical composition of the essential oil of the seven new Lavandula angustifolia and eight new Lavandula x inter...

c-phycocyanin (phycocyanin) is a pigment-protein complex of the light-harvesting phycobiliprotein family that takes part in the primary phase of photosynthesis in lower plants. The phycocyanin content depends on such factors as the species of microalgae, physical state of biomass, extraction techniques, etc. The main methods for obtaining phycocyan...

This review aims to analyze propolis as a potential raw material for the development and manufacture of new health-promoting products. Many scientific publications were retrieved from the Scopus, PubMed, and Google Scholar databases via searching the word "propolis". The different extraction procedures, key biologically active compounds, biological...

The SARS-CoV-2 pandemic affected the entire world and contributed to severe health and economic consequences. A safe and effective vaccine is a tool allowing the pandemic to be controlled. Hence, we aimed to conduct a survey on vaccinations against seasonal influenza and COVID-19 in Poland, Lithuania, and Ukraine. We also evaluated societal attitud...

The yield of essential oils in plants is not high and postdistillation wastes rich in phenolic compounds could be used to enhance the profitability of essential oil-bearing plants. The aim of the study was to evaluate polyphenols in a dry extract obtained from the postdistillation waste of the American basil (Ocimum americanum L.) herb, and to cond...

The DPPH assay provides an easy and rapid way to evaluate the antioxidant activity of herbal preparations. The most important stage of the elaboration of the analytical procedure of total antioxidant activity measured by the DPPH test is to select the volume of an extract or its appropriate dilution for the determination of the reliable total antio...

As a part of the ongoing interest in the nutritional and antioxidative properties of a little-known East Asian plant species, the aim of the study was to determine the contents of macronutrients and selected elements, profiles of fatty and amino acids, the content of phenolic compounds, β-carotene, vitamin A and E, and antioxidant activity of Schis...

Oxidative stress is involved in many diseases. Antioxidants are important as they can delay or stop the formation of free radicals by giving hydrogen atoms or scavenging them [3]. An antioxidant can be deined as any molecule capable of preventing or delaying oxidation of other molecules, usually such as lipids, proteins or nucleic acids [2, 3]. Phy...

Lavender, otherwise known as Lavandula angustifolia Mill., is widely used in landscaping, and its oil is a valuable raw material used in many industries. Therefore, new varieties of this plant are bred. The essential oil composition obtained from fresh flowers of thirteen new Ukrainian cultivars of L. angustifolia were analysed by GC-MS, and eighty...

Due to its vast therapeutic potential, the plant-derived polyphenol curcumin is utilized in an ever-growing number of health-related applications. Here, we report the extraction methodologies, therapeutic properties, advantages and disadvantages linked to curcumin employment, and the new strategies addressed to improve its effectiveness by employin...

The import dependence of the pharmaceutical market of Ukraine on concentrates for hemodialysis determines the relevance of their manufacture. Elaboration of the transfer of drug development to industrial manufacture and, accordingly, the development of industrial technology of liquid acid concentrates for hemodialysis involves scaling the process,...

Plants of the Lamiaceae Martinov family are very useful for people because of their medicinal properties. They are rich in essential oils and polyphenols with valuable healing effects. The development of chromatographic methods for the identification of this family representative's raw materials is a perspective trend for the pharmaceutical industr...

The present study was focused on the chemical composition and antimicrobial activity of the essential oils (EsO) obtained from five Lamiaceae representatives grown in the south of Ukraine. Among them are Salvia sclarea L., Monarda didyma (cultivar ‘Cambridge Scarlet’), Thymus pulegioides (cultivar ‘2/6-07’), Thymus vulgaris (cultivar ‘Jalos’), and...

Сhronic kidney disease (CKD) is the important public and medical problem in the world because of a large burden on health care systems. The prevalence of CKD and number of dialysis patients are increasing in the world. The prevalence and incidence of CKD depends on age, race, and gender of patients, region, and the presence of the CKD registry in a...

Мета роботи. Хроматографічний аналіз ефірних олій монарди лимонної (Monarda citriodora Cerv. ex Lag, родина Lamiaceae Martinov), отриманих із трави рослини в різні фази вегетації. Матеріали і методи. Матеріалом для досліджень була трава M. citriodora, яку заготовляли на дослідних ділянках у Херсонській області на початку цвітіння та у період масов...

This study aimed to determine the composition and content of polyphenols in the dry extract obtained from the hydrodistilled residue by-product of the wild bergamot (Monarda fistulosa L., Lamiaceae Martinov family) herb (MFDE) and to evaluate its safety and pharmacological properties. The total phenolic content (TPC) in the MFDE was 120.64 mg GAE/g...

The component composition of Monarda citriodora (Lamiaceae) essential oils was studied by GC/MS. There were identified 28 components in the essential oil obtained from the herb during the beginning of flowering, and 27 compounds were found during the total flowering of plants. The predominant components of both essential oils were aromatic monoterp...

This study was focused on the chemical composition and antimicrobial activity of the essential oils obtained from the three Thymus species: T. serpyllum L. T. pulegioides L. and T. richardii Pers. The aerial parts of plants were collected in the flowering stage in Kherson region (Ukraine). The isolation of the essential oils was performed by hydrod...

Abstract: Six tinctures were prepared from four different propolis samples of Ukrainian origin. The total flavonoid content (TFC) in the tinctures and propolis samples was determined by the aluminum-chloride method. The TFC of the propolis samples ranged from 4.78% to 7.18% and 11.0% to 19.5% with reference to quercetin and rutin, respectively. The...

GENDER-SPECIFIC FEATURES OF ARTERIAL HYPERTENSION DEVELOPMENT AND TREATMENT IN UKRAINE NATALIIA H. STEPANIUK1, NATALIIA I. HUDZ1and ALLA H. STEPANIUK2 1Danylo Halytsky Lviv National Medical University, Lviv, Ukraine 2National Pirogov Memorial Medical University, Vinnytsya, Ukraine Abstract: Modern cardiology, as well as other fields of medicine, fo...

The aim of this paper is to develop a scientifically based methodology for the development of acid concentrates in order to perform purposefully organized research on the implementation of these concentrates into industrial manufacture. Material and Methods. Methodological concept of acid concentrates development. The objects of the study are liqu...

The aim of the study was chromatographic analysis of essential oil and polyphenols of the hydrodistilled residue by-product developed from the O. americanum herb. Plant raw material was harvested from the experimental plots in Ternopil region (Ukraine) and shade dried. Hydrodistillation method was used to obtain the essential oil from O. americanum...

Proton transfer (PT) reactions, in particular single (SPT), double (DPT) or multiple (MPT), are widespread phenomena in many branches of the life sciences. These reactions proceed through the transfer of the proton between donor and acceptor atoms, in some cases catalyzed by water molecules acting as a connector between these donor and acceptor ato...

Introduction: Summer savory (Satureja hortensis L.) is an annual herbaceous plant belonging to the Lamiaceae family [1, 2]. S. hortensis volatile oils contains such main components as thymol (0.3–28.2%), γ-terpinene (15.30–39%), carvacrol (11–67%), and p-cymene (3.5–19.6%). The herb of S. hortensis is rich in polyphenols [1]. Therefore, this herb c...

Introduction: Species of the Monarda L. genus are the endemics of North America. In recent decades many of them are spread to Europe as cultivated plants with ornamental and promising healing properties [1]. Materials and methods: Compositions of the essential oils hydrodistilated from the flowering aerial parts of four Monarda species (M. citriodo...

Winter Savory (Satureja montana L.) has been used in traditional medicine and as a spice or natural food preservative in the Mediterranean region for centuries. In this paper, some technological and analytical aspects of the S. montana tinctures development and an evaluation of the essential oil composition are provided. The total phenolic and flav...

У світовій медичній практиці спостерігається тенденція до збільшення використання питомої част-ки лікарських засобів, які виготовляються на основі лікарської рослинної сировини. У зв'язку з цим визначено необхідність з'ясувати перспективу подальшого наукового вивчення і застосування рослинної сировини й пре-паратів на основі шавлії лікарської (Salv...

Polyphenols are valuable group of phytoconstituents due to their high antioxidant activity and healing properties. Antioxidant properties of sages are attributed mainly to a high level of phenolic compounds. The aim of the present study was to elaborate an analytical procedure for the evaluation of the content of secondary metabolites of the polyph...

Objectives : To study 5-hydroxymethylfurfural (5-HMF) spectral characteristics aiming at their future application in analytical procedures and their validation for the determination of 5-HMF in liquid products containing glucose after sterilization. Method : Direct spectrophotometric method for the determination of 5-HMF using the molar absorption...

Трава чаберу гірського (Satureja montana L., Lamiaceae) як перспективна лікарська сировина для розробки антимікробних та антиоксидантних засобів Вступ. Для виявлення нових потенційних джерел антимікробних та антиоксидантних рослинних засобів важливе значення має аналіз джерел наукової літератури стосовно фітохімічних та фармакологічних досліджень н...

The objective of this study was to develop and validate an alternative analytical procedure for the total chloride assay in solutions for peritoneal dialysis (PD). The proposed analytical procedure was validated according to the requirements of the International Conference on Harmonization (ICH) Guideline: Topic ICH Q2(R1) and the approach of the S...

Introduction. An analysis of sources of scientific literature concerning phytochemical and pharmacological studies of non-officina medicinal plants is important for the identification of new potential sources of antibacterial and antioxidant plant products. Significant interest in this regard is caused by the biologically active substances of the W...

Water is the most common compound of hydrogen and oxygen in the nature. It is a universal solvent of many substances, and therefore chemically pure water does not exist in the nature. The water contained in the body is qualitatively different from ordinary water as it is structured water. Such crystalline structures of water are the matrix of life....

This paper presents the comparable viability study results of the HepG2 and Vero cells in the presence of traditional peritoneal dialysis (PD) solutions determined by three methods (3-[4,5-dimethylthiazol]-2-yl-2,5-diphenyl tetrazolium bromide (MTT), neutral red (NR) and sulforhodamine B assays) with establishing different correlations between viab...

Sixteen ethanolic extracts were obtained from seven different bee bread samples. The total flavonoid content in the extracts was determined by aluminium-chloride method and was in the range of 8.3 mg/L ± 6.24% to 195.3 mg/L ± 1.35% and 28.8 mg/L ± 19.33% to 603.3 mg/L ± 4.64% with reference to quercetin and rutin, respectively. The relative standar...

The results of the V. agnus-castus, V. negundo and V. negundo var. cannabifolia introduction in the National Botanical Garden by M. M. Hryshko of NAS of Ukraine (Kyiv). The component composition of the essential oils obtained from the flowering shoots of these plants has been established. The prospect of the use of raw material of these plants as a...

На основі аналізу даних інтродукційних досліджень та компонентного складу ефірних олій квітучих пагонів трьох видів роду Vitex встановлено перспективність подальшого фармакологічного вивчення сировини цих рослин як антибактеріальних, антиоксидантних, протизапальних та знеболювальних засобів.

DEAR COLLEAGUE, LET’S TOGETHER DEVELOP FURTHER DISCUSSION OF HONEY AS ZOOM CONFERENCE In view of the growing interest to the treatment properties of HONEY AND BEE PRODUCTS (propolis, bee bread, pollen, royal jelly etc.), with especial focus on antiviral properties, as well as enormous success of our fruitful discussion at Research Gate https://bit...

В статье приведены наиболее распространенные и общепринятые подходы к стандартизации лекарственного растительного сырья, растительных препаратов и продуктов пчеловодства по содержанию суммы флавоноидов с использованием активных маркеров (стандартных образцов) методом спектрофотометрии в видимой области спектра. На основе литературных данных и собст...

The article presents approaches to the development of an analytical procedure of determination of the total flavonoids content by spectrophotometry in the visible spectral range in preparations of the natural origin (herbal preparations and beekeeping products): the choice of analytical wavelength, marker, compensation solution. On the basis of lit...

The present investigation was to estimate the total phenolic content and composition of flavonoids and hydroxycinnamic acids in herbal infusions obtained from aerial parts of three Lamiaceae species (Dracocephalum moldavica, Ocimum americanum and Satureja hortensis). The total phenolic content of herbal infusions was determined using a spectrophoto...

The genus Salvia is one of the largest and the most important aromatic and medicinal genera of the Lamiaceae family and comprises about 900 species, widespread throughout Southeast Asia, Central America and Mediterranean region (Sepahvand et al., 2014). Since ancient times, Salvia sclarea is one of the most appreciated medicinal herbs and widely us...

Glandular trichomes are important taxonomic characters in the Lamiaceae family. The objects of our investigations were plants of Lamiaceae family collected from the experimental base of Institute of Rice of the National Academy of Agrarian Sciences (Plodove, Ukraine). Fresh leaves were collected for the morphological analyse. The dimensions of glan...

The purpose of this study was to quantificate the polyphenol content and antioxidant activity of substances obtained by hot water extraction from Satureja hortensis herb after their drying in a rotary evaporator and freeze drying.

Introduction: Essential oils are the perspective source of natural antioxidants because some synthetic antioxidants are now suspected to be potentially harmful to human health. As free radicals can cause inflammatory diseases, cancer, atherosclerosis, and aging, antioxidants can inhibit oxidative chain reactions. Essential oils have a wide range of...

Objective: The purpose of our work was to conduct technological, analytical, and biological investigations and stability studies of peritoneal dialysis (PD) solutions containing glucose and sodium lactate in single-chamber containers. Methods: Different formulations of PD solutions were prepared and sterilized at a temperature of 121 °C during 15 m...

IDENTIFICATION OF SOME SPECIES OF THYMUS L. FOR MACROSCOPIC FEATURES OF THE LEAF Нrytsyna M.P., Hudz N.I., Svydenko L.V. Differential features for leaves of three species of the Thymus spp. have been established. The largest number of essential oil glands is located on the lower side of the leaves of these species. 2-4 large essential oil glands pe...

SALVIA SCLAREA L. ЯК ПЕРСПЕКТИВНА АКТИВНА СУБСТАНЦІЯ ЛІКАРСЬКИХ ЗАСОБІВ РОСЛИННОГО ПОХОДЖЕННЯ Гудзь Н., Грицина М., Марій Х., Свиденко Л., Григор’єва О., Бріндза Я., Іванішова Е. У роботі представлений огляд літературних джерел стосовно компонентного складу та перспективност застосування ефірних олій шести видів роду Шавлія (Salvia L.): шавлія ліка...

The purpose of our work was to conduct biological and analytical studies of the peritoneal dialysis (PD) solutions containing glucose and sodium lactate and establish correlations between cell viability of the Vero cell line and values of analytical indexes of the tested solutions. The results of this study confirm the cytotoxicity of the PD soluti...

The objective of this work was to estimate glucose degradation products (GDPs) in solutions for peritoneal dialysis (PD) including glucose and sodium lactate based on the change of pH and absorption of ultraviolet (UV) light. Spectrophotometric and pH-metric methods were used to measure glucose degradation and transformation of GDPs in laboratory-m...

The procedure involves monitoring by membrane filtration method using closed-type «Steritest» systems, the amount of the solution from one container meets the requirements of the State Pharmacopeia of Ukraine 2.0 (SPhU), 2.6.1, the number of containers from a batch used for the testing sterility is 20 % of the number of containers in the laboratory...

Pollen contains components necessary for growth and development of a new organism. Polyphenolic compounds are one of them. Pollen phenolic compounds are presented with flavonoids which have different structures and biological functions. Analytical procedure of spectrophotometric method was developed to determine the total flavonoids content in haze...

Flavonoids are regarded as key compounds in bee bread. In this paper, quantitative determination of sum of flavonoids and dominating group of flavonoids were carried out by colorimetric aluminum chloride method. The principle of aluminum chloride colorimetric method is that aluminum chloride forms complexes with flavones and flavanols wherein it re...

Flavonoidcontaining plants attract the attention of researchers due to their prospect in obtaining food products and medicines with various pharmacological effects (anti-inflammatory, vessel protective, choleretic, hepatoprotective, radioprotective, antitumor, immunomodulatory, antimicrobial effects, etc.). For determining the total content of flav...

It was established that phytoadaptogens and immunomodulators are widely used in the composition of dietary food and pharmaceutical products. Phytoadaptogens positively affect the nervous system: normalize the processes of excitation and inhibition in the cerebral cortex, reduce fatigue in physical and mental stress, improve memory, etc. The general...