Natalie Bordag

Natalie Bordag
Medical University of Graz · Department of Dermatology and Venereology

Dr.
Senior Data Scientist

About

56
Publications
7,959
Reads
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1,057
Citations
Introduction
My current research focus is on immunology and metabolomics in skin and pulmnary diseases. Science is my passion. I enjoy working in teams with mutual respect, tolerance, integrity and appreciation of achievements. I love most to drive planning, study design, project management, ethical approval for clinical studies, data pre-processing, quality control, statistical analysis up to machine learning forward so that we can write and present useful results.
Additional affiliations
June 2018 - present
Ludwig Boltzmann Institute for Lung Vascular Research
Position
  • Senior Researcher
April 2015 - June 2019
CBmed GmbH
Position
  • Project Manager
Description
  • project leader of metabolite profiling studies for internal and external contract research, development and improvement of the metabolomics core lab, statistical data analysis, biological interpretation, designing, getting ethical approval and leading a human clinical study, scientific dissemination, raising public and company third-party funding
February 2014 - March 2015
BASF Österreich GmbH
Position
  • Researcher
Description
  • project leader of metabolite profiling studies for internal and external contract research, statistical data analysis, biological interpretation, scientific dissemination, knowledge management
Education
July 2006 - November 2011
Humboldt-Universität zu Berlin
Field of study
  • Biophysics "Thermodynamics of membrane protein folding of the model Mistic"
October 2000 - November 2005
University of Leipzig
Field of study
  • Biochemistry "Biochemical and physical characterisation of nanoparticles as drug carrier through the blood brain barrier"

Publications

Publications (56)
Article
Full-text available
The bleomycin mouse model is the extensively used model to study pulmonary fibrosis; however, the inflammatory cell kinetics and their compartmentalization is still incompletely understood. Here we assembled historical flow cytometry data, totaling 303 samples and 16 inflammatory-cell populations, and applied advanced data modeling and machine lear...
Article
Full-text available
Emerging evidence indicates that disruption of the gut microbial community (dysbiosis) impairs mental health. Germ-free mice and antibiotic-induced gut dysbiosis are two approaches to establish causality in gut microbiota-brain relationships. However, both models have limitations, as germ-free mice display alterations in blood-brain barrier and bra...
Article
Full-text available
Liver toxicity is a leading systemic toxicity of drugs and chemicals demanding more human-relevant, high throughput, cost effective in vitro solutions. In addition to contributing to animal welfare, in vitro techniques facilitate exploring and understanding the molecular mechanisms underlying toxicity. New ‘omics technologies can provide comprehens...
Article
Caloric restriction and intermittent fasting are known to prolong life- and healthspan in model organisms, while their effects on humans are less well studied. In a randomized controlled trial study (ClinicalTrials.gov identifier: NCT02673515), we show that 4 weeks of strict alternate day fasting (ADF) improved markers of general health in healthy,...
Article
Full-text available
Glucocorticoids are indispensable anti-inflammatory and decongestant drugs with high prevalence of use at ∼ 0.9% of the adult population. Better holistic insights into glucocorticoid-induced changes are crucial for effective use as concurrent medication and management of adverse effects. The profiles of 214 metabolites from plasma of 20 male health...
Article
Full-text available
The transition from the fetal to the neonatal circulation includes dilatation of the pulmonary arteries (PA) and closure of the Ductus Arteriosus Botalli (DAB). The resting membrane potential and various potassium channel activities in smooth muscle cells (SMC) from fetal and neonatal PA and DAB obtained from the same species has not been systemati...
Article
Full-text available
The regulator of G protein signaling (RGS) represents a widespread system of controllers of cellular responses. The activities of the R4 subfamily of RGSs have been elucidated in allergic pulmonary diseases. However, the R4 signaling in other inflammatory lung diseases, with a strong cellular immune response, remained unexplored. Thus, our study ai...
Article
Full-text available
Timely centrifugation of blood for plasma preparation is a key step to ensure high plasma quality for analytics. Delays during preparation can significantly influence readouts of key clinical parameters. However, in a routine clinical environment, a strictly controlled timeline is often not feasible. The next best approach is to control for sample...
Preprint
Full-text available
PESI-MS enables with its greatly simplified handling and fast result delivery the application field for high-throughput use in routine settings. In health care and research, pre-analytical errors often remain undetected and disrupt diagnosis, treatment, clinical studies and biomarker validations incurring high costs. This proof-of-principle study i...
Preprint
Full-text available
Prolonged incubation of blood prior to plasma preparation can significantly influence the quality of the resulting data. Different markers for this pre-clinical variability have been proposed over the years but with limited success. In this study we explored the usefulness of glutathione (GSH) status, namely ratio of reduced to oxidized glutathione...
Preprint
The bleomycin mouse model is the most extensively used animal model to study pulmonary fibrosis. Despite this, the inflammatory cell kinetics and cell compartmentalisation is still incompletely understood. Here we simultaneously analysed 16 inflammatory cell populations in 303 samples and applied advanced data modelling to conclusively detail these...
Article
Full-text available
The kinase AKT2 (PKB) is an important mediator of insulin signaling, for which loss-of-function knockout (KO) mutants lead to early onset diabetes mellitus, and dominant active mutations lead to early development of obesity and endothelial cell (EC) dysfunction. To model EC dysfunction, we used edited human pluripotent stem cells (hPSCs) that carri...
Article
Rationale: Approximately 50% of heart failure patients are diagnosed with Heart Failure with preserved Ejection Fraction (HFpEF), but there are currently no effective treatments. Hypothesis: Treatment of a feline HFpEF animal model with a pan HDAC inhibitor, SAHA, will improve the cardiopulmonary phenotype and mediate changes in the metabolome and...
Article
Melanoma cells shift between epigenetic-metabolic states to adapt to stress and, particularly, to drugs. Here, we unraveled the metabolome of an H3K4 demethylase (KDM5B/JARID1B)-driven melanoma cell phenotype, which is known to be multi-drug resistant. We set up a fast protocol for standardized, high sensitive liquid-chromatography-high-resolution-...
Article
Influenza A virus is a pathogen responsible for severe seasonal epidemics threatening human and animal populations every year. One of the ten proteins encoded by the viral genome, the matrix protein M1, is abundantly produced in infected cells and plays a structural role in determining the morphology of the virus. During assembly of new viral parti...
Article
Full-text available
Eicosanoids are lipid mediator molecules with key roles in inflammatory skin diseases, such as psoriasis. Eicosanoids are released close to the source of inflammation, where they elicit local, pleiotropic effects and dysregulations. Monitoring inflammatory mediators directly in skin lesions could provide new insights and therapeutic possibilities....
Book
Full-text available
This book will present globally the main results and findings from the facts and figures collected by the different workpackages through the analysis of the literature, through the interviews and from the tools developed in the project. This material was enriched by the inputs from the external experts we have invited in the two conferences of cons...
Preprint
Full-text available
Influenza A virus is a pathogen responsible for severe seasonal epidemics threatening human and animal populations every year. One of the ten proteins encoded by the viral genome, the matrix protein M1, is abundantly produced in infected cells and plays a structural role in determining the morphology of the virus. During assembly of new viral parti...
Conference Paper
Full-text available
Introduction Tyrosine kinase inhibitors (TKI) have dramatically changed the prognosis of patients with leukaemia. Unfortunately, they fail to achieve durable remissions for many patients with advanced BCR-Abl leukaemia or acute myelogenous leukaemia. Myeloid leukemic cells depend on mitochondrial oxidative phosphorylation (OxPhos) and/or glycolysis...
Article
Full-text available
Glucocorticoids (GCs) are important regulators of systemic energy metabolism, and aberrant GC action is linked to metabolic dysfunctions. Yet, the extent to which normal and pathophysiological energy metabolism depend on the GC receptor (GR) in adipocytes remains unclear. Here, we demonstrate that adipocyte GR deficiency in mice significantly impac...
Article
Full-text available
The metabolome offers real time detection of the adaptive, multi-parametric response of the organisms to environmental changes, pathophysiological stimuli or genetic modifications and thus rationalizes the optimization of cell cultures in bioprocessing. In bioprocessing the measurement of physiological intracellular metabolite levels is imperative...
Data
Key characteristics of the filters and the consecutively tested criteria are summarized. (DOCX)
Data
ANOVA read out comparing all centrifuged samples versus all fast filtrated samples. (XLSX)
Article
The MetaMap®-Tox database contains plasma-metabolome and toxicity data of rats obtained from oral administration of 550 reference compounds following a standardized adapted OECD 407 protocol. Here, metabolic profiles for aniline (A), chloroform (CL), ethylbenzene (EB), 2-methoxyethanol (ME), N,N-dimethylformamide (DMF) and tetrahydrofurane (THF), d...
Article
Full-text available
Metabolomics is a valuable tool to gain mechanistic insight into biological processes. It is frequently used to obtain complementary details to other ‘omics technologies such as transcriptomics or proteomics. For knowledge generation, reproducible measurements of physiological, intact, and artifact-free metabolite levels are imperative necessitatin...
Article
Full-text available
The interaction of the B. subtilis protein Mistic with the bacterial membrane as well as its role in promoting the overexpression of other membrane proteins are still matters of debate. In this study, we aimed to determine whether individual helical fragments of Mistic are sufficient for its interaction with membranes in vivo and in vitro. To this...
Article
Full-text available
Alpha-helical membrane proteins fulfill many vital roles in all living cells and constitute the majority of drug targets. However, their relevance is in no way paralleled by our current understanding of their structures and functions. This is because membrane proteins present a number of experimental obstacles that are difficult to surmount by clas...
Article
Full-text available
For the delivery of drugs into the brain, the use of nanoparticles as carriers has been described as a promising approach. Here, we prepared nanoparticles as carriers for the model drugs thioflavin T and thioflavin S that bind fibrillar amyloid beta peptides (Abeta). These polymer colloids are composed of a polystyrene core and a degradable PBCA [p...
Conference Paper
Lateral diffusion within membranes plays an important role for essential cellular processes as, e.g., membrane formation and inter-/intracellular signalling. We model the diffusion of a protein represented by a charged polystyrene latex within a lipid monolayer at the air-water interface mimicking a cell membrane with spatial inhomogeneities - e.g....

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