Natalia Naumova

Russian Academy of Sciences | RAS · Laboratory of Agrochemistry

Backgound/Objective: Novel compounds for mitigating globally growing microbial resistance to antibiotics have been recently more actively researched. Triviron is a polycationic amphiphile synthetic compound with a ribonuclease activity and is used as an antiviral in veterinary medicine. Methods: We studied the effect of triviron on the mouse (line...

Composting is an environmentally friendly process, turning animal waste into fertilizer. Chicken litter compost (CLC) improves soil properties, increasing crop yields. However, the CLC effect on the soil microbiome is understudied. This study aimed to compare bacteriobiome diversity in fallow arable Chernozem with and without CLC addition in a fiel...

The aim of the study to investigate the effect of individual or combined K and Mg addition on potato tuber yield and quality along the gradient of soil and environmental conditions, which was formed by the five experimental plots in the forest-steppe one of West Siberia. Location and time of the study. Microplot field experiment with moderately ear...

Background. The search for more efficient pro- and prebiotics to improve calves’ health, including decreasing the incidence of intestinal infections and increasing biomass production, has been very actual. The effect of prebiotics, however, is poorly, especially as related to gut microbiome biodiversity. The aim of this work was to study the influe...

Microeukaryotes are vital for maintaining soil quality and ecosystem functioning, however, their communities are less studied than bacterial and fungal ones, especially by high throughput sequencing techniques. Alveolates are important members of soil microbial communities, being consumers and/or prey for other microorganisms. We studied alveolate...

The aim of the study was to exmine the effct of low rate of potassium humate application with and without fly ash (FA) on wheat roots rhizosphere bacteriobiome. Location and time of the study. The microplot field experiment was conducted in three replicates in randomized design in 2022 in Novosibirsk (Russia). Methods. Soil physical and chemical pr...

Managing soil biodiversity using reduced tillage is a popular approach, yet soil bacteriobiomes in the agroecosystems of Siberia has been scarcely studied, especially as they are related to tillage. We studied bacteriobiomes in Chernozem under natural steppe vegetation and cropped for wheat using conventional or no tillage in a long-term field tria...

This brief review focuses on the properties of bioaerosols, presenting some recent results of metagenomic studies of the air microbiome performed using next-generation sequencing. The taxonomic composition and structure of the bioaerosol microbiome may display diurnal and seasonal dynamics and be dependent on meteorological events such as dust stor...

Agricultural practices can affect root-associated microbiota, but the effect of fertilization is still poorly examined. The aim of this study was to obtain 16S and ITS metagenomic profiles of tomato rhizosphere and root endosphere under mineral (NPK) fertilization in the open field experiment in the south of West Siberia. We found 6 bacterial and 3...

Managing soil biodiversity by reduced or no tillage is an increasingly popular approach. Soil mycobiome in Siberian agroecosystems has been scarcely studied; little is known about its changes due to tillage. We studied mycobiome in Chernozem under natural steppe vegetation and cropped for wheat by conventional or no tillage in a long-term field tri...

A culture-independent mycobiome survey in Haplic Cambisol under Korean pine in a long-term field experiment in the Russian Far East was conducted using sequence analysis of the ITS region amplified with ITS3/ITS4 primers using the metagenomic DNA as a matrix. Overall 758 fungal OTUs were identified, representing 15 phyla, 47 classes, 104 orders, 18...

As the information about the effect of pectin prebiotics on chicken gut microbiota is scarce, by using high throughput metagenomic sequencing with Illumina Miseq we examined the cloaca bacteriobiome of male and female chickens receiving antibiotic- or pectin-containing drinking water. The bacteriobiome was dominated by two phyla (Firmicutes and Pro...

The article describes research and teaching activity of Denis Alexandrovich Gavrilov, Candidate of Biological Sciences, senior researcher with Soil Genesis Laboratory of the Institute of Soil Science and Agrochemistry of the Siberian Branch of the Russian Academy of Sciences, who died suddenly died young on August 26, 2021. He was also and enthusia...

Imbalance of nutrients limits crop yields. Although K fertilization receives sufficient attention in research and practice, Mg supply is rather neglected. The effect of Mg fertilization (0, 5 and 10 g Mg/m2), combined with two K fertilization rates (10 and 15 g K/m2), on potato production and soil exchangeable K and Mg was studied in a three-season...

The aim of the study. The aim of the study was to review publication about microbiome of chicken manure, chicken manure compost, as well as soil and crop microbiome after compost addition to soil as a fertilizer. Methodology. A search in the bibliographical data bases PubMed and elibrary.ru was performed using the keywords pertaining to the topic o...

Fly ash dumps of thermal power stations have been increasing in area worldwide due to the growing demand for power. One of the cost-effective approaches for restoring such sites is spontaneous revegetation in those areas where adjacent indigenous ecosystems can supply seeds and living organisms. We assessed microbiome taxonomic diversity in a Techn...

The intestinal health of poultry is of great importance for birds’ growth and development; probiotics-driven shifts in gut microbiome can exert considerable indirect effect on birds’ welfare and production performance. The information about gut microbiota of ducks is scarce; by using high throughput metagenomic sequencing with Illumina Miseq we exa...

The last decade saw extensive studies of the human gut microbiome and its relationship to specific diseases, including gallstone disease (GSD). The information about the gut microbiome in GSD-afflicted Russian patients is scarce, despite the increasing GSD incidence worldwide. Although the gut microbiota was described in some GSD cohorts, little is...

Vast areas of land in the forest-steppe of West Siberia are occupied by birch forests, the most common ecosystems there. However, currently, little is known about the microbiome composition in the underlying soil, especially along a sequence of soil genetic horizons. The study aimed at inventorying microbiome in genetic horizons of a typical Phaeoz...

The aim of the study. The aim was to profile 16S rRNA gene diversity and to assess functional potential of bacterial assemblages in the rhizosphere of some unconventional vegetables grown in protected greenhouse conditions in West Siberia. Location and time of the study. Novosibirsk, Russia, 2016. Methodology. At the end of the growing season in th...

The multiple sclerosis (MS) incidence rate has been increasing in Russia, but the information about the gut bacteriobiome in the MS-afflicted patients is scarce. Using the Illumina MiSeq sequencing of 16S rRNA gene amplicons, we aimed to analyze the Firmicutes phylum and its taxa in a cohort of Moscow patients with relapsing-remitting MS, assessing...

The article discusses some examples of incorrect methodology and terminology practice in soil research and publications. In particular, the authors draw attention to extremely inflated and unjustified use of the phrase “statistically significant”, to the controversy between the chemical determination and terminological definition of soil humus, to...

Gut microbiota is believed to play a crucial role in modulating obesity in humans, and probiotics affecting gut microbiota can alleviate some of the obesity-related health complications. The study was aimed to investigate changes in the composition of the gut microbiome in obese humans due to short-term (2 weeks) treatment of obese patients with a...

Background: Gut microbiota has been increasingly acknowledged to shape significantly human health, contributing to various autoimmune diseases, both intestinal and non-intestinal, including multiple sclerosis (MS). Gut microbiota studies in patients with relapsing remitting MS strongly suggested its possible role in immunoregulation; however, the...

Background. The aim of the study was to assess the composition and structure of bacterial assemblages by estimating 16S rRNA gene sequences diversity in a young Technosol, developing on a revegetating fly ash dump. Location and place of the study. Two soils were chosen for the study in the environs of the thermoelectric power station No.5 in Novosi...

The composition and structure of rhizosphere bacteriobiome of the husk tomato ( Physalis philadelphica Lam.) plants grown on Phaeozem in the open field in West Siberia, Russia (55°15’ NL, 83°31’ EL) were studied using Illumina MiSeq sequencing of the V3-V4 hypervariable region of 16S rRNA genes. In total 5898 OTUs (Operational Taxonomic Units) were...

Husk tomato (Physalis philadelphica Lam.) a source of functional food and medicinal compounds, has attracted renewed interest for production in temperate zones. Field-grown husk tomato yield and fruit properties and their relationship with soil chemistry and temperature were studied in the south of West Siberia, Russia, at five experimental sites....

The article describes results of the microplot field trial to investigate potential use of metallurgical slags for fracture of capillary rim during reclamation of toxic waste dumping areas, such as enrichment plants tailings, solid waste dumps, etc., with minimal possible introduction of fertile soil layer. Such approach allows energy-effective uti...

In a microplot field trial, the use of crushed slag to break down the capillary borders in recultivation of toxic tailings (at enrichment facilities and solid waste dumps) with minimum application of fertile soil was studied. In this approach, metallurgical wastes may be used in low-cost energy-saving technologies. Four basic types of slag produced...

Potato cultivars have different strategies for dealing with potassium (K) deficiency in soil, and their response to different forms and rates of K fertilisation may vary because of differences in soil K availability. This study was performed to evaluate the effect of K fertilisation rates (0, 30, 60, 90, 120 and 150 kg K/ha) on tuber yield and qual...

The results of estimation of growth and development parameters, SSR analysis, electron microscopic examination of the surface of pollen and seeds, biochemical composition (ascorbic acid, carotenoids, carotenes, macro- and microelements content in the stem part of plant) of 8 Chinese broccoli, Brassica oleracea var. alboglabra samples grown in the u...

The initial stage of biogeocenoses development on the coal ash dump produced by the thermal power staton in Novosibirsk (55.000, 83.068), Russia, were studied after 9 years of spontaneous revegetation. Soil properties, soil cover and plant communities were examined in detail. The predominating types of embryozems and transition from open to success...

Kiwano plants, or horned cucumber (Cucumis metuliferus E. Mey ex Naudin) was grown in the open field in several experimental plots in the south of West Siberia (54,95025 N, 83,09763 E). Fruits production was correlated with night air temperature sum and soil organic matter content, reaching the maximum of 2.7 kg (fresh phytomass) per plant. Fruit s...

Bacterial diversity was assessed by sequencing SSU 16S rRNA gene fragments (V3-V4 hyper-variable region) obtained from the metagenomic DNA extracted from the rhizosphere soil of the two pine species (Pinus sibirica Du Tour and Pinus koraiensis Sieboldd et Zucc.) in the long-term field provenance experiment in Krasnoyarsk region (Russia). Both pines...

The content of macro- (C, N, P, S) and micro-elements, as well as some metals was analyzed in fruits, leaves, stems, and roots of vigna, kiwano, bitter melon, and wax gourd growing in the condition of unheated polyethylene-covered greenhouse in Novosibirsk (Russia). The revealed low concentration of sulfur and big atomic ratio of nitrogen to sulfur...

In the long-term (30 years) field provenance experiment (Novosibirsk, Russia) with Scotch pine Pinus sylvestris L. from different geographical populations, X-ray fluorescence analysis with synchrotron radiation (XRFSRA) was used to estimate the concentration of 16 chemical elements in different components of the phytomass collected from living tree...

By using multivariate statistical analysis, the influence of Scots pine climatypes on a set of chemical and microbiological properties of soil, i.e., soil C/N, Cmic/Corg, and Cmic/Nmic, was revealed in a series of long-term (ca. 30 years) field experiments, which were carried out according to the same scheme under contrasting environmental and soil...

Archived soil samples are a valuable source for retrospective ecological studies, and their recent analysis using molecular ecological approaches has drawn significant attention within the scientific community. However, the possibility of addressing ecological questions regarding detectable microbiota in dried and extensively stored soils has not y...

The method of X-ray fluorescence analysis with synchrotron radiation was used to estimate soil and phytomass elemental composition in the long-term field experiments with clones and climatypes of Scots pine Pinus sylvestris L. in West Siberia. Overall from 15 to 21 chemical elements (Fe, Ca, K, Ti, Mn, Zr, Sr, Rb, Co, Cr, Zn, V, Y, Sc, Nb, Pb, Ga,...

We measured phytomass stock and production in Western Siberian mire ecosystems (palsa, ridge, oligotrophic and mesotrophic hollows, fen). To determine the contribution of different phytomass fractions into total production, we developed a method to estimate below-ground production (BNP). Standing crop of living above-ground phytomass on treeless pl...

The biomass and activity of the microorganisms were studied in the soil and litter under a pine forest two years after a surface fire. The data obtained showed that the differences between the disturbed and undisturbed microbiotopes were mainly displayed in the litter with respect to the CO2 emission and the metabolic activity of the microbial comm...

A topic not covered in the recent special Section on Soils: The Final Frontier (11 June, pp. 1613–1637) is the possibility of using modern DNA-based molecular techniques to study microbial diversity in archived soil samples. Like other soil research institutes in countries such as the United

Bacterial communities in genetic horizons of undisturbed podzols were found to differ quantitatively (in biomass and population) and qualitatively (in the morphology of viable cells and oxidation of carbohydrates, oxycarbonic acids, and phenol derivatives). Methods of multisubstrate testing and fluorescence microscopy were used.

A topic not covered in the recent special Section on Soils: The Final Frontier (11 June, pp. 1613-1637) is the possibility of using modern DNA-based molecular techniques to study microbial diversity in archived soil samples. Like other soil research institutes in countries such as the United Kingdom, the United States, and Switzerland, Alterra, as...

Simulating the evolution of both 14C and 12C-CO2 in the rhizoplane was monitored during the diffusion of 14C-labelled glucose, oxalic acid, or glutamic acid into soil from a filter placed on the surface of a sandy loam. After 3 and 7 d, soil was sampled from four layers (0–2, 2–4, 4–6, and 6–14 mm) to determine residual 14C in each layer. The miner...

The denaturing gradient gel-electrophoresis of bacterial DNA fragments and the assessment of bacterial biomass revealed changes in the diversity of the bacterial community in a meadow alluvial soil upon long-term fertilization.

The activity of three enzymatic systems of xenobiotic metabolism (cytochrome P-450-dependent monooxygenases, non-specific esterases and glutathione S-transferases) was studied in sensitive (S) and resistant to tetrametrin (Rtetr.), permetrin (Rperm.), mecarbenyl (Rmec.) and chlorophos (Rchlor.) strains of the housefly M. domestica L. In Rtetr. and...