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Natalia Kozyrovska

Natalia Kozyrovska
Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine · Laboratory of Microbial Ecology

PhD

About

115
Publications
36,870
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Introduction
We have initiated a new project to study a role of extracellular membrane vesicles (EMV) in microbial community of bacteria and yeast known as kombucha. We hypothesized that beneficial properties of the kombucha drink are determined in large part by its EMVs. We also hypothesized that kombucha's products will be beneficial for astronauts and workers under “expedition” type of dwelling conditions. In this project, we are focused on safety of postbiotic component – EMVs - for macroorganism under stressful conditions.
Additional affiliations
August 1976 - present
National Academy of Sciences of Ukraine
Position
  • Principal Investigator
Description
  • Interrelationships of microbial communities with macroorganisms, and a role of membrane vesicles in these interactions

Publications

Publications (115)
Article
Full-text available
The increased antibiotic resistance of pathogenic bacteria requires intense research of new wound healing agents. Novel wound dressings should be designed to provide wound disinfection, good moisture, and fast epithelization. In this study, bacterial cellulose (BC) was impregnated with graphene quantum dots (GQDs) for potential use in wound healing...
Article
Kombucha mutualistic community (KMC) is composed by acetic acid bacteria and yeasts, producing fermented tea with health benefits. As part of the BIOlogy and Mars EXperiment (BIOMEX) project, the effect of Mars-like conditions on the KMC was analyzed. Here, we analyzed metagenomeassembled genomes (MAGs) of the Komagataeibacter, which is a predomina...
Article
The spread of antibiotic resistance is becoming a serious global health concern. Numerous studies have been done to investigate the dynamics of antibiotic resistance genes (ARGs) in both indoor and outdoor environments. Nonetheless, few studies are available about the dynamics of the antibiotic resistome (total content of ARGs in the microbial cult...
Article
Full-text available
Komagataeibacter is the dominant taxon and cellulose-producing bacteria in the Kombucha Microbial Community (KMC). This is the first study to isolate the K. oboediens genome from a reactivated space-exposed KMC sample and comprehensively characterize it. The space-exposed genome was compared with the Earth-based reference genome to understand the g...
Article
Full-text available
Humankind has entered a new era of space exploration: settlements on other planetary bodies are foreseen in the near future. Advanced technologies are being developed to support the adaptation to extraterrestrial environments and, with a view on the longer term, to support the viability of an independent economy. Biological processes will likely pl...
Article
Full-text available
Kombucha is a traditional tea fermented by symbiotic microbiota, and it has been known as a functional fermented product. Here, we report four microbial metagenome-assembled genome sequences (MAGs) reconstructed from the microbiomes in kombucha exposed to a Mars-like environment outside the International Space Station.
Article
Since the pathogenic bacteria biofilms are involved in 70% of chronic infections and their resistance to antibiotics is increased, the research in this field requires new healing agents. New composite hydrogels were designed as potential chronic wound dressings composed of bacterial cellulose (BC) with chitosan polymer (Chi) – BC-Chi and chitosan n...
Article
Full-text available
Endophytic bacteria, recognized for their beneficial effects on plant development and adaptation, can facilitate the survival of Antarctic plants in severe environments. Here we studied endophytes of the vascular plant Deschampsia antarctica Ė. Desv. from two distantly located regions in the maritime Antarctic: King George Island (South Shetland Is...
Article
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Cellulose is a widespread macromolecule in terrestrial environments and a major architectural component of microbial biofilm. Therefore, cellulose might be considered a biosignature that indicates the presence of microbial life. We present, for the first time, characteristics of bacterial cellulose after long-term spaceflight and exposure to simule...
Article
Therapy of bacterial urinary tract infections (UTIs) and catheter associated urinary tract infections (CAUTIs) is still a great challenge because of the resistance of bacteria to nowadays used antibiotics and encrustation of catheters. Bacterial cellulose (BC) as a biocompatible material with a high porosity allows incorporation of different materi...
Article
Bacterial infection and their resistance to known antibiotics delays wound healing. In this study, nanochitosan dots (nChiD) produced by gamma irradiation have been encapsulated in bacterial cellulose (BC) polymer matrix to study the antibacterial potentials of these nanocomposites and their possible usage in wound healing treatment (scratch assay)...
Article
Kombucha is a multispecies microbial ecosystem mainly composed by acetic acid bacteria and osmophilic acid‐tolerant yeasts, which is used to produce a probiotic drink. Furthermore, Kombucha Mutualistic Community (KMC) has been recently proposed to be used during long space missions as both a living functional fermented product to improve astronauts...
Conference Paper
Full-text available
In recent years interest has grown in the results of biological studies of astromaterials. The main source of such biological information is meteorites, which belong to the class of carbonaceous chondrites. For solutions of these issues need to develop a clear concept for detecting possible nano-vesicles in micrometeorites. We believe that such ast...
Article
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Outer membrane vesicles (OMVs), produced by nonpathogenic Gram-negative bacteria, have potentially useful biotechnological applications in extraterrestrial extreme environments. However, their biological effects under the impact of various stressors have to be elucidated for safety reasons. In the spaceflight experiment, model biofilm kombucha micr...
Article
Full-text available
Rice is a major staple food across the globe. Its growth and productivity is highly dependent on the rhizobiome where crosstalk takes place between plant and the microbial community. Such interactions lead to selective enrichment of plant beneficial microbes which ultimately defines the crop health and productivity. In this study, rhizobiome modula...
Preprint
Full-text available
The vascular plant Deschampsia antarctica samples were collected for endophytic bacteria study from two regions in the maritime Antarctic 400 km distant from one another: Point Thomas oasis (King George Island) and Argentine Islands (Galindez Island). The endophytes were isolated from roots and leaves of D. antarctica, cultivated and identified by...
Article
A kombucha multimicrobial culture (KMC) was exposed to simulated Mars-like conditions in low Earth orbit (LEO). The study was part of the BIOlogy and Mars EXperiment (BIOMEX), which was accommodated in the European Space Agency’s EXPOSE-R2 facility, outside the International Space Station. The aim of the study was to investigate the capability of a...
Article
Full-text available
BIOMEX (BIOlogy and Mars EXperiment) is an ESA/Roscosmos space exposure experiment housed within the exposure facility EXPOSE-R2 outside the Zvezda module on the International Space Station (ISS). The design of the multiuser facility supports—among others—the BIOMEX investigations into the stability and level of degradation of space-exposed biosign...
Article
Full-text available
A kombucha multimicrobial culture (KMC) was exposed to simulated Mars-like conditions in low-Earth orbit (LEO). The study was part of the Biology and Mars Experiment (BIOMEX), which was accommodated in the European Space Agency's EXPOSE-R2 facility, outside the International Space Station. The aim of the study was to investigate the capability of a...
Article
Full-text available
Regenerative medicine therapy is inspired by current research advances in cellular biology, genetic engineering, synthetic biology, material sciences and so far contributes to the traditional therapy, making resistant diseases curable. Nowadays, chronic wound healing is possible due to cell-based regenerative technologies and recent non-cell therap...
Article
Lignins and lignin-derived compounds are known to have antibacterial properties. The wound healing agents in the form of dressings produce faster skin repair and decrease pain in patients. In order to create an efficient antimicrobial agent in the form of dressing in the treatment of chronic wounds, a composite hydrogel of bacterial cellulose (BC)...
Conference Paper
Full-text available
Microorganisms combine archaebacteria (or archaea), bacteria and eukaryote. The first two are characterized by prokaryotic cell structure. They lack a cell nucleus, and nuclear DNA has the form of a molecule enclosed in a ring, and is localized directly in the cytoplasm. In the cells of the eukaryote there is a nucleus and other organelles that are...
Article
New protic ionic liquid N-butylguanidinium tetrafluoroborate (BG-BF4) has been synthesized by single-step method. The ionic liquid is thermally stable to at least 300 °C and has ionic conductivity value of 8⋅10⁻³ S/cm at room temperature increasing to 0.18 S/cm at 180 °C. To develop a new proton conducting membrane, the nano-fibrillar matrix of bac...
Article
Full-text available
Biofilm-forming microbial communities are known as the most robust assemblages that can survive in harsh environments. Biofilm-associated microorganisms display greatly increased resistance to physical and chemical adverse conditions, and they are expected to be the first form of life on Earth or anywhere else. Biological molecules synthesized by b...
Article
Full-text available
Kombucha microbial community (KMC) produces a cellulose-based biopolymer of industrial importance and a probiotic beverage. KMC-derived cellulose-based pellicle film is known as a highly adaptive microbial macrocolony - a stratified community of prokaryotes and eukaryotes. In the framework of the multipurpose international astrobiological project "...
Conference Paper
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Endophytic bacteria are ubiquitously found inside of all plant species (Rosenblueth & Martı´nez-Romero, 2006), and create, together with the host plant, a superorganism – a multispecies community that functions as an organizational unit (Podolich et al, 2015). Endophytes may play additional roles in the adaptation of plants to extreme conditions. P...
Article
Bacteria rely mainly on enzymes, glutathione and other low-molecular weight thiols to overcome oxidative stress. However, hydroxyl radicals are the most cytotoxic reactive oxygen species, and no known enzymatic system exists for their detoxification. We now show that methyl-esterified dimers and trimers of 3-hydroxybutyrate (ME-3HB), produced by ba...
Article
Full-text available
High-throughput sequencing allows obtaining DNA barcodes of multiple species of microorganisms from a single environmental sample. Next Generation Sequencing (NGS)-based profiling provides new opportunities to evaluate the human health effect of microbial community members affiliated to probiotics. DNA metabarcoding may serve as a quality control o...
Article
Full-text available
A novel polymer-electrolyte membrane based on bacterial cellulose saturated with protic ionic liquid triethylammonium trifluoromethylsulfonate was obtained. The thermogravimetric analysis revealed that bacterial cellulose/triethylammonium trifluoromethylsulfonate composite is thermally stable up to 260 °С. The specific conductivity of the membrane...
Article
Full-text available
Introducing of the DNA metabarcoding analysis of probiotic microbial communities allowed getting insight into their functioning and establishing a better control on safety and efficacy of the probiotic communities. In this work the kombucha poly-microbial probiotic community was analysed to study its flexibility under different growth conditions. E...
Article
Full-text available
Introducing of the DNA metabarcoding analysis of probiotic microbial communities allowed getting insight into their functioning and establishing a better control on safety and efficacy of the probiotic communities. In this work the kombucha poly ‑ microbial probiotic community was analysed to study its flexibility under different growth condition...
Article
Full-text available
Kombucha is a multispecies microbial community which produce bacterial cellulose — a polymer molecule to be a candidate for a biomarker of life. For the pre-flight ground-based phase of Biology and Mars Experiment (BIOMEX), the multi-microbial cellulose-based biofilm was embedded in mineral material to test the structural integrity of the bacterial...
Article
Full-text available
Aims The effect of microbial preparations on plant growth and resistance to diseases depends on plant genotype and growth conditions limiting their use in agriculture. Various biocontrol effects, reported for the endophyte Methylobacterium sp. IMBG290, are possibly modulated through the endophyte community of the potato (Solanum tuberosum L.) host...
Article
Full-text available
The acetic acid bacteria have mainly relevance for bacterial cellulose production and fermented bio-products manufacture. The purpose of this study was to identify temperate bacteriophages in a cellulose-producing bacterial strain Komagataeibacter intermedius IMBG180. Prophages from K. intermedius IMBG180 were induced with mitomycin C and nalidixic...
Article
Full-text available
Background From the point of view of systems biology, the plant is considered a super organism that consists of the plant per se and numerous populations of pro- and eukaryotic microbial organisms. Each plant species hosts a genotype-specific core microbiome, dynamically responding to environmental cues, such as soil quality. In the plant endosphe...
Article
Full-text available
Bacterial cellulose (BC) is a matrix for a biofilm formation, which is critical for survival and persistence of microbes in harsh environments. BC could play a significant role in the formation of microbial mats in pristine ecosystems on Earth. The prime objective of this study was to measure to what extent spectral and other characteristics of BC...
Article
One important mechanism for understanding the interaction between cells and the various radiation sources in space is to perform laboratory analysis on microorganisms or bio-relevant molecules which have been exposed to this radiation environment. However comprehensive scientific assessment of the biological effects caused by exposure to real space...
Article
Full-text available
Plants are heavily populated by pro- and eukaryotic microorganisms and represent therefore the tremendous complexity as a biological system. This system exists as an information processing entity with rather complex processes of communication, occurring throughout the individual plant. The plant cellular information processing network constitutes t...
Data
Full-text available
Probiotics are essential for establishing and maintaining optimal immune health. The probiotic therapy is known from alternative medicine for ages; however, the recent demonstration of the normal microflora to induce innate immunity has introduced the science-based concept of therapeutic application of potentially beneficial probiotic microorganism...
Article
Full-text available
Bacteria are micro- and nanoorganisms that spatially colonize the plant organism and comprise various types of interactions with the host ranging from pathogenic to mutualistic and symbiotic. The presence of these exo- and endosymbionts may affect the plant host physiology. Non-pathogenic bacteria can enhance defensive capacity in plants that provi...
Article
Full-text available
Plant inoculation with endophytic bacteria that normally live inside the plant without harming the host is a highly promising approach for biological disease control. The mechanism of resistance induction by beneficial bacteria is poorly understood, because pathways are only partly known and systemic responses are typically not seen. The innate end...
Data
Data matrices used for AMMI from three (Fig 1B, 1C, 3B) and five (Fig. 2B) T-RFLP replicates. Mb = T-RF corresponding to Methylobacterium, B = potato cv. Blue Congo, T = Timo, P = Pito, M = Matilda, m = Methylobacterium inoculation, number indicates inoculation density (log10 CFU ml−1), S = shoot, R = root, * = challenge inoculation. (DOC)
Data
Effect of Methylobacterium inoculation on disease resistance of potato. Resistance of (A) in vitro-grown potato cvs. Blue Congo, Timo, Pito, Matilda (B, T, P, M) to Phytophthora infestans and (B) greenhouse-grown potato cvs. Bellarosa (Br) and Yavir (Y*) towards P. infestans (Pi) and Pectobacterium atrosepticum (Pa). Methylobacterium sp. IMBG290 wa...
Data
Summary of the experiments performed. (DOC)
Article
The Low Earth Orbit (LEO) experiment Biology and Mars Experiment (BIOMEX) is an interdisciplinary and international space research project selected by ESA. The experiment will be accommodated on the space exposure facility EXPOSE-R2 on the International Space Station (ISS) and is foreseen to be launched in 2013. The prime objective of BIOMEX is to...
Chapter
Full-text available
Heavy metals and metalloids have become one of the major environmental concerns which pose a serious threat to plants and animal health. In this context, endophytic bacteria could play an important role in understanding the uptake mechanism of heavy metal ions and providing immunity to plant against metal toxicity. The defensive effects of certain...
Article
The LowEarthOrbit(LEO)experiment Biology and Mars Experiment(BIOMEX)isaninterdisciplinary and internationalspaceresearchprojectselectedbyESA.Theexperimentwillbeaccommodatedonthe space exposurefacilityEXPOSE-R2ontheInternationalSpaceStation(ISS)andisforeseentobe launchedin2013.TheprimeobjectiveofBIOMEXistomeasuretowhatextentbiomolecules,suchas pigme...
Article
Full-text available
Probiotics are essential for establishing and maintaining optimal immune health. The probiotic therapy is known from alternative medicine for ages; however, the recent demonstration of the normal microflora to induce innate immunity has introduced the science-based concept of therapeutic application of potentially beneficial probiotic microorganism...
Article
Full-text available
Naturally occurring symbiotic microbial communities (SMK) are the most robust assemblages for a multipurpose use in keeping humans healthy and soil fertile. Especially, safe and reliable SMK are needed for producing probiotics and ferments valuable for health problems prophylaxis. This is true for long-term expeditions, outposts, extraterrestrial p...
Article
Full-text available
Microorganisms may be a key element in a precursory scenario of growing pioneer plants for extraterrestrial exploration. They can be used for plant inoculation to leach nutritional elements from regolith, to alleviate lunar stressors, as well as to decompose both lunar rocks and the plant straw in order to form a protosoil. Bioleaching capacities o...
Conference Paper
The ability of plants to protect themselves against ionizing radiation is limited. One of the way how to alleviate irradiation consequences in plants is to use plant-associated bacteria for inoculation. Two defined plant growth promoting bacterial strains were used for inoculation Kalanchoe daigremontiana (Hamet & Perrier) plantlets before acute ir...
Article
Full-text available
Inoculating plants that have inefficient antioxidant systems with plant-associated bacteria allows them to overcome heavy metal intoxication. We monitored protein oxidation, the activity of plant defense system enzymes, and the phenolics content in soybean (Glycine max L.) during a prolonged exposure to cadmium (Cd). The assistance of the bacterial...
Article
Full-text available
Aim. To screen mobile genetic elements (MGE) in the bacterium which caused decay of field-grown onion bulb and to study an integron and gene cassettes associated. Methods. Polymerase chain reaction (PCR) and PCR products sequencing were used for both the bacterium and MGE identification. Terminally-labeled Restriction Fragment Length Polymorphism (...
Conference Paper
Full-text available
Precursory lunar lander experiments on growing plants in locker-based chambers will increase our understanding of effect of lunar conditions on plant physiology. The inducible CAM (Crassulacean Acid Metabolism)-plants are reasonable model for a study of relationships between environmental challenges and changes in plant/bacteria gene expression. In...
Article
Full-text available
Photosynthesis is useful for biosignature definition, for the definition of the habitability of a planet, for research on the likelihood of Panspermia and for use in manned space flight missions by integration into life supporting systems.
Article
Full-text available
The ability of plants to protect themselves against ionizing radiation is limited. One of the way how to alleviate irradiation consequences in plants is to use plant-associated bacteria for inoculation. Two defined plant growth promoting bacterial strains were used for inoculation Kalanchoe daigremontiana plantlets before acute irradiation with g-q...