Nasrin Moshtaghi

• Ferdowsi University of Mashhad

Algal bioprospecting in ecosystems leads to exploring native microalgae and the competency evaluation of economically producing lipids as biofuel or nutritional applications. In this study, ten microalgae species were screened from the saline water lake. Chlorella vulgaris, Chlorella sorokiniana, Chlamydomonas raudensis, Chlamydomonas hedleyi, Duna...

The present study aimed to investigate the effects of different concentrations of NaCl on membrane stability, potassium (K ⁺ ) to sodium (Na ⁺ ) ratio, and the expression of stress-responsive genes in aerial and underground organs of O. sativa cv. IR64 and Aeluropus littoralis to shed more light on the salt stress adaptive mechanisms in the grass s...

Mentha longifolia L. (horsemint) is one of the most famous wild perennial herbs of the Lamiaceae family, which has broad usage in therapeutic and pharmaceutical services. In this research, screening of twenty Mentha longifolia L. accessions was assessed according to the physio-chemical variability under low water irrigation. The investigation was a...

Understanding gene expression kinetics and the underlying physiological mechanisms in stress combinations is a challenge for the purpose of stress resistance breeding. The novelty of this study is correlating the physiological mechanisms with the expression of key target genes in tall fescue under a combination of various salinity and osmotic stres...

Algal bioprospecting in ecosystems leads to exploring native microalgae and the competency evaluation of economically producing lipids as biofuel or nutritional applications. In this study, ten microalgae species were screened from the saltwater lake. Chlorella vulgaris , Chlorella sorokiniana , Chlamydomonas raudensis , Chlamydomonas hedleyi , Dun...

Unlabelled: Somatic embryogenesis (SE) depends on a variety of developmental pathways that are influenced by several environmental factors. Therefore, it is important to understand the relationship between environmental and genetic factors by identifying the gene networks involved in SE through gene set enrichment analysis (GSEA). For determinatio...

Background: Datura innoxia Mill. is one of the medicinal plant which can produce tropan alkaloids such as hyoscyamine and scopolamine with analgesic and antiasthmatic activities. Objective: In this research, the effect of various factors such as culture media and bacteria strain were studied on hairy roots induction and secondary metabolites produc...

Several Salvia species are among the most valuable aromatic herbs used for industrial and pharmaceutical applications. Hence, under greenhouse trial conditions, the effects of chitosan treatment (0, 100, and 200 mg L⁻¹) and water deficiency stress on the morphological, physiological, and biochemical features of two Salvia species were examined. Sig...

Hairy root culture is a promising approach to improve production of plant secondary metabolites. The genes, which are located in T-DNA of a root-inducing plasmid, regulate auxin sensitivity of hairy roots. Therefore, this study was aimed to improve the growth and rosmarinic acid production of Lavandula angustifolia hairy roots. Lateral branches of...

Background: Datura species produce a wide range of tropane alkaloids with medicinal values. D. innoxia is a species of the Datura genus which is poisonous and has an unpleasant odor due to the presence of tropane alkaloids. Objective: The study aimed to induction of polyploidy by colchicine treatment on downy thorn-apple and the effect of it on app...

Background: Thalictrum minus L. is a medicinal plant contains berberine, which is used for complementary treating of some diseases. Excessive harvesting of this species from rangelands to extract the alkaloid has endangered its survival. Objective: This study was aimed for in vitro propagation of this species and berberine production. Methods: The...

Background: Papaver bracteatum Lindl. (Persian poppy) has a very long history as the major source of natural alkaloids. Among Papaver species, Persian poppy can be considered as a rich source of thebaine. Objective: The purpose of this study was to evaluate the thebaine content in different organs and different populations of Persian poppy. Methods...

مصرف انرژی و تاثیر آن بر محیط‌زیست یکی از بزرگترین چالش‌های بشر است و تولید اتانول زیستی یا سایر مواد شیمیایی به وسیله هیدرولیز آنزیمی سلولز می‌تواند تاثیر مهمی در جایگزینی سوختهای فسیلی و کاهش تولید CO2 در اتمسفر داشته باشد. آنزیم Cel6B از باکتری گرمادوست Thermobifidia fusca، یک CBHII متعلق به خانواده بی سلولازهاست که تا C° 55 مقاوم به حرارت است....

Mentha longifolia L. is well‐known to be one of the most pervasive wild‐growing species of the Lamiaceae family, which has extensive beneficial properties in the fields of pharmacology and biological products. In the present study, the correlation between Inter‐simple sequence repeat (ISSR) markers and morpho‐chemical parameters of twenty different...

Pyrus communis is a difficult-to-root species, and its optimal rooting depends on applying an accurate concentration of various chemicals. Since statistical modeling can be applied to estimate the approximate range of factors needed for the success of in vitro plant propagation, we tried to optimize the rooting medium using response surface methodo...

Carbon source plays an important role in the induction of embryogenic callus (EC). Therefore it is necessary to determine the effective carbohydrate for improving this process. In this study, different carbohydrates as sucrose, maltose, and sorbitol with different levels (0–30 g/L) were tested in the callus induction of Pyrus communis. At first, in...

Mentha longifolia L. is one of the most valuable perennial, rhizomatous, aromatic and herbaceous plants belonging to the Lamiaceae family. In this study, the effects of deficit irrigation stress on physiological traits and yield of 20 different wild mint populations were evaluated by a split-plot experiment based on a randomized complete block desi...

The effects of plant growth regulators were investigated on Kalanchoe blossfeldiana regeneration and then gene transferring was optimized. Three types of explants: leaf surface, vein and petiole and different concentrations of Benzyl Adenine (BA) and Kinetin (Kin) alone or in combination with Naphthalene Acetic Acid (NAA) were used for regeneration...

Endosperm culture can be considered a method to achieve polyploid plants. An experiment was conducted to determine the ploidy levels in leaf cells of plantlets originating from the endosperm and cotyledonary segment explants after organogenesis. The ploidy level of plantlets was determined by flow cytometry. Also, anatomical and morphological trait...

Nanoelicitors are biological and non-biological factors that can affect the synthesis of secondary metabolites in medicinal plants. Feverfew is a valuable medicinal plant containing effective and important anti-cancer compounds (essential oil and parthenolide). This study was conducted to investigate the effects of zinc oxide nanoparticles (ZnONPs)...

Glycyrrhiza glabra (licorice) is a medicinal plant with valuable specialised metabolites such as triterpene sweetener glycyrrhizin. Salinity stress is the main environmental stress limiting plant growth and development. The effects of six levels of NaCl (0, 100, 200, 400, 600, and 800 mM) on growth, osmolyte content, oxidative stress markers, antio...

This chapter includes the methods for tissue and cell culture of saffron. Saffron is a triploid plant, and therefore vegetative reproduction is a common way for its propagation. In traditional culture, few cormlets are produced each year and thus the production rate is low. Tissue culture methods such as micropropagation, somatic embryogenesis, org...

Largescale propagation of Pyrus communis, which is a difficultto root species, is dependent on tissue culture technique. However, internal bac terial contaminations are an obstacle in tissue culture of fruit tree species. For this purpose, this investigation was conducted with several experiments to manage bacterial contamination. First, gram test...

Introduction: Nowadays, different solvents are used to extract plant antioxidants. Each solvent has a special ability to extract antioxidants materials. Accordingly, in this research, some medicinal herbs were collected from different vegetation zones of Iran and evaluated based on the majority of useful solvents. The best solvent for the extractio...

The Cel B enzyme from Thermobifidia fusca, a CBHII, belongs to the family of cellulase B, which is highly resistant to heat. Due to the low level of production of this enzyme in this host, it cannot be used at industrial scale. For expression of cellobihydrolase in yeast, cel B gene in pSZ plasmid was amplified by PCR and then was cloned in to pPIC...

Feverfew (Tanacetum parthenium L. Schultz Bip.) has a lot of secondary metabolites, such as phenols, essential oils and sesquiterpene lactones (parthenolide) which have high value in chemotaxonomy and medicine. This study was carried out to evaluate the phytochemical potential of Feverfew. Parthenolide was identified by UPLC-MRM-MS. The essential o...

Plants are exposed to solar ultraviolet radiation due to use of sunlight for photosynthesis. Additionally, salinity in soil or water influences the plant productivity and quality considerably. Moreover, when plants are simultaneously exposed to multiple stresses, one form of stress can affect the response to other stress. Particularly, it has been...

There is an urgent need to develop novel drugs for osteoporosis which occurs due to estrogen deficiency. Phytoestrogens derived from medicinal plants would be the best alternative to chemical drugs with harmful side effects. The main purpose of the present study was to investigate the effect of ferutinin compared to 17β-estradiol (E2) on bone miner...

There is a growing body of the literature that recognizes the importance of ferutinin (C22H30O4) as one of the natural phytoestrogens with potency to treat osteoporosis and some kind of cancers. One of the greatest challenges is availability of ferutinin that is found just in root of some plants of the genus Ferula (Apiaceae), which is the reason o...

In order to understand the effects of ultraviolet-B (UV-B) radiation and salinity stress on the visual quality and morphological parameters, one-year-old seedlings of rosemary (Rosmarinus officinalis L.) were grown under three UV-B levels (0, 4.32, 6.05 kJ m⁻² d⁻¹) and four salinity regimes (Control, 50, 100 and 150 mM NaCl) under greenhouse condit...

Objective(s) The purpose of this study was to evaluate variations in yields, volatile composition and biological activities of essential oils (EOs) obtained from the aerial parts of Zhumeria majdae collected from five localities of the south of Iran. Materials and Methods The EOs were analyzed using gas chromatography and gas chromatography-mass s...

In this research, agro-morphological variation among twenty genotypes of Mentha longifolia L. was assessed in 2 years based on agricultural production under water stress condition. The experiment was arranged in split–split-plot design with water stress treatment in two irrigation levels of 100% (as control) and 60% field capacity in three replicat...

Basil is a culinary herb and an edible vegetable of the family Lamiaceae (mints). There are many varieties of Ocimum basilicum, as well as several related species or hybrids also called basil. This investigation was carried out in 2017, measuring the growth, flowering, and essential amount of ten selected varieties. A greenhouse experiment was carr...

Basil is a culinary herb and an edible vegetable of the family Lamiaceae (mints). There are many varieties of Ocimum basilicum, as well as several related species or hybrids also called basil. This investigation was carried out in 2017, measuring the growth, flowering, and essential amount of ten selected varieties. A greenhouse experiment was carr...

Catharanthus roseus is known as the only source for the low-abundance anticancer agents namely vinblastine and vincristine. Fine tuning of accumulation of such secondary metabolites is highly governed by the regulatory genes. Among these genes, Catharanthus roseus MYC1 (CrMYC1) is known as one of the key transcription factors regulating the biosynt...

The influenza A virus is of global concern for the poultry industry, especially the H5 subtype as it has the potential to become highly pathogenic for poultry and mankind. Recently, plant expression systems have gained interest as an alternative for the production of vaccine antigens. The goal of the present study was to investigate the possibility...

Kalanchoe blossfeldiana R. is a pot plant with red, pink and white flowers and fleshy leaves. In this study, the effect of plant growth regulators were investigated on plant regeneration and then gene transferring was optimized. In the first experiment, three types of explants: Leaf, veins and petioles were used for regeneration and different conce...

The impending influenza virus pandemic requires global vaccination to prevent large-scale mortality and morbidity, but traditional influenza virus vaccine production is too slow for rapid responses. In this study, bacterial system has been developed for expression and purification of properly folded HA1 antigen as a rapid response to emerging pande...

Among abiotic stresses, salinity has been increasing over the time for many reasons like using chemical fertilizers, global warming and rising sea levels. Under salinity stress, the loss of water availability, toxicity of Na+ and ion imbalance directly reduces carbon fixation and biomass production in plants. K+ is a major agent that can counteract...

Foot and Mouth Disease (FMD) is a very dangerous livestock disease which causes a serious loss in the production of milk and meat. Therefore, producing an effective recombinant subunit vaccine virus this disease is of great importance. Transient gene expression is a valuable tool to reach rapid and acceptable recombinant vaccine. An Agrobacterium-m...

Among abiotic stresses, salinity has been increasing over the time for many reasons like using chemical fertilizers, global warming and rising sea levels. Under salinity stress, the loss of water availability, toxicity of Na + and ion imbalance directly reduces carbon fixation and biomass production in plants. K + is a major agent that can countera...

1. Background Foot-and-Mouth Disease Virus (FMDV) is a causative agent of foot and mouth disease, a highly contagious disease adversely affect-ing milk and meat-producing animals around the world, particularly in developing coun-tries (1). The G-H loop of VP1 capsid pro-tein of FMDV, covering residues 134-158, has been reported by many researchers...

A chimeric construct of foot and mouth disease virus capsid protein VP1 was expressed in Chlamydomonas reinhardtii via Agrobacterium-mediated genetic transformation. Integration of the foreign gene in nuclear genome of the algae was confirmed by PCR and expression of the recombinant protein was confirmed at translational level. Results showed that...

Expressed sequence tags simple sequence repeats (EST-SSRs) are important sources for investigation of genetic diversity and molecular marker development. Similar to genomic SSRs, the EST-SSRs are useful markers for many applications in genetics and plant breeding such as genetic diversity analysis, molecular mapping and cross-transferability across...

The plant vacuolar Na+/H+ antiporter plays an important role in salt tolerance. A vacuolar Na+/H+ cDNA (LfNHX1) gene was cloned from Leptochloa fusca(L.) Kunth, a halophyte forage grass, by cDNA and RACE methods using degenerate primers. The results show that the LfNHX sequence contains 2452 nucleotides and an open-reading frame of1620 nucleotides...

Vacuolar H+-translocation pyrophosphatase (V-PPase) and Na+/H+ antiporter (VNa +/H+) are important transporters in plant cells and have essential roles against abiotic stresses. In this study, the effects of salt stress were surveyed on the transcription of VPPase pump and Na+/H+ antiporter genes at early times of NaCl treatment in roots of halophy...

Eukaryotic NHX transporters are trans-membrane proteins which are vital for cellular homeostasis. Plants vacuolar NHX antiporters have been suggested to be involved in sequestering Na+ into vacuoles, thus preventing toxic effects of Na+ in the cytoplasm. Primary and secondary structure analysis of NHX antiporter of Leptochloa fusca suggested that t...

In this paper, the effects of a number of growth regulators as well as supplements to the Murashige and Skoog (MS) basal medium were evaluated on the regeneration of Alstroemeria rhizome explants. In the first experiment the effects of three cytokinins (BA, TDZ and 2IP each at 0.5, 1 and 2 mg/l) in combination with NAA (0.2 mg/l), followed by anoth...

Saffron (Crocus sativus) is the most valuable and indigenous crop in Iran. The stigmas of flower are used as a popular natural flavouring, colouring and medicinal agent. However, the market suffers from frauds in this plant such as mixing with safflower petals due to high profit. Identification of these frauds with conventional and biochemical meth...

Leaves, one year old stems and buds were used as explants. WPM, B5, MS, half and one-fourth strength MS with 2,4-D, NAA, IBA, IAA and picloram 2, 5 and 10 mg/l were used for callus induction. Investigation into the seasonal effect on callus regeneration and explants browning was carried out in mid-April until September. Results showed that washing...

Freezing stress as one of the abiotic stresses limits the plant growth in some areas. Therefore, increasing the tolerance to freezing stress is necessary for surviving in winter. Plant diversity can be produced by mutation in plant breeding programs. This research was carried out with the purpose of applying gamma-ray as a mutagen for screening fre...

In this study karyotype and chromosome characteristics of the nine accessions of Aegilops crassa species obtained from gene bank of Seed and Plant Improvement Research Institute (SPII) of Iran and one accession collected by authors were inspected. Aceto-iron-hematoxilin staining method was used to stain chromosomes. For each accession, chromosome c...

Saffron (Crocus sativus L.) is the most valuable and indigenous plant in Iran. But knowledge on the molecular investigations of saffron is still limited. The importance of this plant is due to red stigmas with color, flavor and medicinal properties. Saffron stigmas contain a lot of carotenoids such as Crocetin and Crocin. Some genes such as bch and...

Betaine as an osmoprotectant has an important role in plant tolerance in abiotic stresses. Therefore, the effect of exogenous application of betaine on increasing tolerance to cold and freezing tolerance of two chickpea genotypes were studied. Electrolyte leakage was used in this study for determination of injury percentage. This experiment carried...

In this study, multiple shoot induction and whole plant regeneration from decapitated embryo axes of three chick peal genotypes including MCC252, MCC283 and MCC505 were evaluated on modified Murashige and Skoog's medium (MMS) which, its vitamins were replaced by vitamins of B5 medium, supplemented with varied concentration of thidiazuron (0.1, 0.2...