Nashi Widodo

Brawijaya University · Department of Biology

Oxidative stress and inflammation have a role in the development of various diseases. Oxidative stress and inflammation are associated with many proteins, including Kelch ECH associating protein 1 (KEAP1) and inducible nitric oxide synthase (iNOS) proteins. The active compounds contained in Holothuria scabra have antioxidant and anti-inflammatory p...

nSARS-CoV2 causes pneumonia and acute respiratory distress syndrome that involves exacerbated pro-inflammatory cytokines hyperproduction. ZICURMA, zing-curcuma is a tonic developed from ginger, turmeric, and curcuma that may be adequate to remedy nSARS-CoV2 infections. Therefore, this study aims to predict the effectiveness and possible inhibition...

The hypocholesterolemic activity of soy isoflavones has been studied, but the exact mechanism underlying the activity remains unclear. This study reveals the proposed mechanism of the cholesterol-lowering effect of soy isoflavones by computational simulations. Daidzin, Glycitin, Genistin, Daidzein, Glycitein, Genistein, Glyceollin I, Glyceollin II,...

In our previous study, aurone compound were found in the n-butanol fraction of Sterculia quadrifida leaves. The aurone compound (compound 1) had high cytotoxic activity in cell lines with IC50 values of 4.05, 12.53, 15.38, and 25.91 μg/mL in 4T1, MCF7, MDA-MB-435, T47D, respectively. In this study, we performed the MMP-9 enzyme inhibition test with...

This study investigates the synthesis, chemical, and physical properties of isothiocyanate poly(methyl eugenol) or IPME and thiosemicarbazide poly(methyl eugenol) or TPME. The IPME synthesis as a precursor was firstly carried out by reacting Methyl Eugenol (ME) with potassium hydrogen sulfate and potassium thiocyanate in chloroform solution at room...

In our previous study, aurone compounds were found in the n-butanol fraction of Sterculia quadrifida leaves. These compounds had high cytotoxic activity in cell lines with IC50 values of 4.05, 12.53, 15.38, and 25.91 μg/mL in 4T1, MCF7, MDA-MB-435, T47D, respectively. In this study, we performed the MMP-9 enzyme inhibition test with FRET assay, mol...

Context: Breast cancer most commonly occurs in women globally and has the highest mortality rate in Asia. Therefore, a safe and prominent drug to cure the disease needs to be urgently developed. Aims: To investigate the molecular mechanism of the ethanol extract of Curcuma xanthorrhiza (ECx) ininducing apoptosis in breast cancer cell line T47D. M...

Dietary intervention plays a significant role in regulating hyperlipidemia. Besides, elicited soybean has greater bioactive contents with numerous health benefits potential. However, there is no evidence of the hypolipidemic activity of elicited soybean. This study will explore the potential mechanism of hypolipidemic activity of bioactive compound...

Background: Elephantopus scaber and Phaleria macrocarpa have recently been interested as novel anticancer agents. However, there was no scientific evaluation of the anticancer effect of both plant combinations. Objective: This study investigated the potential anticancer effects of combined E. scaber and P. macrocarpa leaves extract on human brea...

Breast cancer is a malignant cancer with annually increasing incidence and mortality rates. The anticancer effect of Curcuma zedoaria (Berg.) Roscoe ethanol extract (ECz) on T47D breast cancer cells was investigated in vitro. The ECz contains phenolic and flavonoid compounds that may play a critical role in anticancer activity. The viability of the...

Background: Turmeric ( Curcuma longa ) has high potential as a traditional anticancer drug. This study aimed to analyze the anticancer activity of turmeric ethanol extract on T47D cells and examine the interaction of Akt1 protein with compounds contained in turmeric. Methods : The cytotoxicity assay was conducted using WST-1 reagents. Apoptosis ass...

Breast cancer is the most common type of cancer women suffer from worldwide in 2020 and the 4th leading cause of cancer death. Boesenbergia rotunda is an herb with high potential as an anticancer agent. This study explores the potential bioactive compounds in B. rotunda as anti-breast cancer agents using in silico and in vitro approaches. The in si...

Tyrosine kinase inhibitors (TKIs) are widely used in cancer treatment due to their effectiveness in cancer cell killing. However, an off-target of this agent limits its success. Cardiotoxicity-associated TKIs have been widely reported. Tyrosine kinase is involved in many regulatory processes in a cell, and it is involved in cancer formation. Recent...

Multidrug treatment has been piloted for Plasmodium falciparum malaria infection; however, multidrug resistance requires serious attention. Therefore, new antimalarial studies have been intensively carried out, including research on new compounds containing nitrogen and sulfur atoms that are predicted as active groups against antiplasmodial. This s...

Plasmodium falciparum malaria contributes to significant global diseases. Computer-aided drug design, screening, and discovery were used to analyze a novel series of Methyl Eugenol Benzaldehyde Thiosemicarbazone (MEBThi) and Methyl Eugenol Cinnamaldehyde Thiosemicarbazone (MECThi) derivatives for malaria falciparum inhibition. This present study sh...

Background: The standardization and mechanism of action of Caesalpinia sappan as an anticancer agent are still lacking. This study aimed to understand the mechanism of action of C,sappan extract as an anticancer agent. Methods: This study was conducted using the A549 lung cancer cell line to understand the mechanism of action of C. sappan extract a...

Serine racemase (SR) catalyzes L-serine racemization to activate the N-methyl-D-aspartate receptor (NMDAR). NMDAR activation is associated with the progression of acute-to-chronic neuropathic pain. This study aimed to investigate NMDAR antagonist interactions with SR to obtain potential chronic pain target therapy. Several NMDAR antagonist drugs we...

Phaleria macrocarpa is a medicinal plant widely used in Indonesian folk medicine to treat several diseases, including cancer. However, the comparative evaluation of various plant parts of P. macrocarpa has not been studied for their anticancer properties on breast cancer. The study aimed to assess the antiproliferative activity of the ethanol extra...

Spatholobus littoralis Hassk has biological activities such as strong antioxidants, booster of Leydig cell enhancer and testosterone level booster in male Rattus norvegicus, wound healer on the white mouse incision and cytotoxic against cell culture MCF7, HepG2, T47D, WIDR, HTB, and HeLa. This present study predicts bioactive compounds from S. litt...

Severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2), the causative agent of coronavirus disease-19 (COVID-19), has become a global health issue. Spike proteins from the virus have a vital role in infection. Herbal medicines such as Lagerstroemia, Euphorbia hirta, and Kleinhovia hospita have several pharmacological functions such as antican...

Cancer is a major health problem worldwide and the leading cause of death in many countries. It remains challenging to find anticancer treatments that work efficiently for varying types of cancer cells. Several studies revealed that nuclear factor kappa B (NF-κB) is a family of dimeric transcription factors that induce tumor promotion, progression,...

Ragi Tape (RT) is commonly used as the microbial starter for various Indonesian traditional food. Consist of diverse microbial populations, RT has excellent agents as elicitors to augment the bioactive compounds in Soybean (SB) and Mungbean (MB). Metabarcoding analysis has shown to identify the microbial population involved in elicitation using RT....

Spatholobus littoralis Hassk (SLH) plant grows wild in the forests of the island of Kalimantan. This plant has the potential as an antioxidant and anticancer. The current research was to quantify the total phenol content, measure the antioxidant and anticancer power against breast cancer cells in non-polar (hexane), polar (water), and semi-polar (e...

A combination of herbals medicines is an alternative treatment choice for developing anticancer therapy because of its benefits, active compounds, and non-toxic side effect. This study investigates the anticancer and antioxidant activity of Phyllanthus urinaria and Curcuma longa water extract combination. The analysis of their bio-active components...

Background: The standardization and mechanism of action of Caesalpinia sappan as an anticancer agent are still lacking. This study aimed to understand the mechanism of action of C,sappan extract as an anticancer agent. Methods: This study was conducted using the A549 lung cancer cell line to understand the mechanism of action of C. sappan extract a...

The development of apoptotic agents from natural plants has potential as promising breast cancer treatment candidates. The study on the efficacy of plants of the genus Spatholobus on breast cancer is still very limited. This study aims to explain the molecular mechanism that underlies the biological activity of breast anticancer from plants of the...

Current cancer treatment methods are still inadequate due to the complexity of the cancer progression mechanism, which involves multiple genes, proteins, and signaling pathways. The discovery and validation of novel anticancer compounds remains challenging. Garlic has many medicinal properties that can combat various diseases. Organosulfur present...

Cenderawasih Bay has extremely high biodiversity with an enormous amount of endemic species due to geography isolation. Tripneustes gratilla is one of the species that is abundant in the bay. The species has ecological value that is suitable for bio-indicators of the environmental condition. Since the Bay relatively isolated area, then we examined...

Article Info Importance of the work: The mechanism of cancer escape from immune system elimination is mediated by the PD-L1/PD-1 protein interaction. Objectives: To investigate the potential of a bioactive compound of Cyperus rotundus L. rhizome extract (CRE) as an inhibitor of PD-L1/PD-1 interaction. Materials & Methods: The plant extraction was c...

Sterculia quadrifida is empirically used as a medicinal plant. The leaves of the plant are reported to have anti-infective, antioxidant, and anti-cancer activities. This study aims to identify active compounds from the plant leaves by bioassay-guided isolation. S. quadrifida leaves were extracted with 80% methanol, then partitioned with n-hexane, c...

Salmonella typhi is a foodborne pathogenic bacterium that threatens health. S. typhi infection exacerbated the antibiotic resistance problem that needs alternative strategies. Moringa oleifera possesses anti-inflammatory and antimicrobial effects. However, there is a lack of information about the pharmacological value of red M. oleifera. The fermen...

Previously, elicitation using microbe consortium inside Ragi Tape (RT) has given some insight with better results than mono-starter elicitation. However, the exact microbe species involved during elicitation remain unknown. This research aimed to identify the microbe community structure before and after elicitation using RT. Soybean (SB) and Mung B...

Hepatitis B virus (HBV) is one of the causes to induce liver chronic disease until liver cancer. The virus is inserted and integrated through the host’s DNA. It affected cell cycles improperly. Microarray technology is a tool to investigate gene expression by quantifying RNA in the liver cancer mechanism. However, the number of genes involved is ve...

Background: Metabolic syndrome is a significant risk factor for cardiovascular diseases. Green tea and green coffee extracts, antioxidant and anti-inflammatory agents may participate in metabolic syndrome-induced cardiac fibrosis alleviation. However, the effect of combination of those extracts still needs exploration. Therefore, this study investi...

Background : Insulin resistance has been independently associated with cardiac diseases. A free fatty acid is recently known to induce cardiac insulin resistance due to low-grade inflammation. Therefore, the improvement of free fatty acid levels can also improve cardiac insulin resistance. This study investigated the combination of green tea and de...

Breast cancer is the most common type of cancer in women globally. The overexpressed proteins, including EGFR, PI3K, AKT1, and CDK4, have a role in the growth of breast cancer cells. The 3D peptide structure of sea cucumber Cucumaria frondosa was modeled and then docked with EGFR, PI3K, AKT1, and CDK4 proteins using AutoDock Vina software. The dock...

Breast cancer is the most common type of cancer in women globally. The overexpressed proteins, including EGFR, PI3K, AKT1, and CDK4, have a role in the growth of breast cancer cells. The 3D peptide structure of sea cucumber Cucumaria frondosa was modeled and then docked with EGFR, PI3K, AKT1, and CDK4 proteins using AutoDock Vina software. The dock...

Context: The development of apoptotic agent from natural plant products may have a beneficial effect as a promising candidate for cancer therapy. The study about the efficacy of Phaleria macrocarpa leaves on breast cancer is still limited. Aims: To elucidate the molecular mechanisms underlying the anti-breast cancer activity of P. macrocarpa leave...

Traditionally, noni (Morinda citrifolia L.) has been used to treat hypertension in tropical countries. The noni extract was proven to reduce blood pressure and relatively safe to the liver and kidney in the animal model. This extract could inhibit angiotensin-converting enzyme (ACE) and plays a pivotal role in controlling blood pressure. However, t...

Background : Individually, green tea and green coffee have been extensively studied for mitigation of metabolic syndrome (MS) in both rats and humans; however, their combined effect requires further investigation. Thus, we compared the metabolic effect of combining green tea and decaffeinated light roasted green coffee on MS in rats. Methods: An MS...

Several reports have suggested that glutathione (GSH) has antibacterial activity. However, the mechanism by which GSH inhibits microbial growth is a mystery. GSH has a structure similar to the antibiotic precursors of Penicillium that work as inhibitors of the d-alanyl-d-alanine-carboxypeptidase (DacC) enzyme. DacC catalyzes glycopeptides (d-alanyl...

During growth, cattle increase in weight, size, and also in muscle tissue proportions. Wide variability of carcass traits can be used as base selection on cattle growth. Carcass traits are economical traits that influenced by a complex network of gene interactions in the muscle, and tools such as RNA Sequencing can be used to identify gene interact...

The variation in semen quality serves to determine the fertility potential of Ongole Crossbred (PO) bulls. Semen quality has a correlation to fertility. Individual motility is one of most important factors determining the success of artificial insemination program. The aims of this research were to mapping the bulls who have high and low fertility...

This study aimed to determine the antioxidant activity and potential cytotoxicity effects of crude ethanol extract of Mahkota Dewa (Phaleria macrocarpa) leaves on breast cancer cell lines. The antioxidant potential of crude extract of P. macrocarpa was carried out by 2,2-diphenylpicrylhydrazyl (DPPH) assay. The total phenolic and flavonoid contents...

The mammalian/mechanistic target of rapamycin (mTOR) is a serine/threonine kinase which commonly hyperactivated in the majority of cancers. mTOR was reportedly involved in various tumorigenesis and cancer development. Cancer therapy, combined with mTOR inhibitors, can be a good strategy for cancer treatment. Biflavonoids from Selaginella doederlein...

Background The classification of active compounds based on their function using machine learning is essential for predicting the function of new active compounds quickly. These classification results are beneficial and accelerate the work of laboratory assistants in identifying the function of active compounds. In this study, an active compound is...

The study aimed to analyze the potency of Cyperus rotundus bioactive compounds to inhibit the anti-apoptotic protein Bcl-2 and Bcl-xl by in silico approach. Ten bioactive compounds were used in this study, such as apigenin, aureusidin, cyperol, cyperusol A1, cyperusol B2, cyperusol D, luteolin, methyltartonic, quercetin, and scaberin. The 3D struct...

Selaginella doederleinii Hieron is a wild plant that has long been used as a traditional anticancer drug in Indonesia. The majority of its anticancer activity comes from biflavonoids. However, the potency of the biflavonoids as anticancer agents for hormone receptor-positive (HR+) breast cancer, the most common type of cancer in Indonesia has never...

The objective of this study was to evaluate the total phenolic and flavonoid contents and to investigate antioxidant and cytotoxic activities of ethanol extract of pericarp, mesocarp and seeds of Phaleria macrocarpa (Scheff.) Boerl. The total phenolic content was determined as Gallic acid (GAE) equivalent, and flavonoid contents were determined as...

MicroRNAs (miRNAs) are noncoding RNA molecules that play a significant role in atherosclerosis pathogenesis through post-transcriptional regulation. In the present work, a bioinformatic analysis using TargetScan and miRdB databases was performed to identify the miRNAs targeting three genes involved in foam cell atherosclerosis (CD36, SOCS1, and Vav...

Sterculia foetida leaf extract has been shown to have cytotoxic activity. Matrix metalloproteinase-9 (MMP-9) has an important role in pathophysiological functions. Inhibition of MMP9 is an important therapeutic approach for combating cancer. This study was conducted to determine the most active fraction of S. foetida as anti-breast cancer agent wit...

Snake venom phospholipase A2s (svPLA2s) has been known as the most abundant component and predominant cause of Russell’s viper envenomation. Limitation to serum therapy and considerable pharmacological interest led the researcher to synthesized multi-toxic PLA2 inhibitors, δ2-isoxazolines derivate. Although δ2- isoxazolines derivate already proved...

Breast cancer may cause a death due to the late diagnosis. A cheap and accurate tool for early detection of this disease is essential to prevent fatal incidence. In general, the cheap and less invasive method to diagnose the disease could be done by biopsy using fine needle aspirates from breast tissue. However, rapid and accurate identification of...

Background Liver cancer is often associated with hepatitis B infection, and there is a progression from chronic hepatitis to hepatocellular carcinoma. The replication of the virus affects the cell cycle of the host. Many oncogenes that express themselves in the formation of proteins can be highly expressed. A microarray technique can be used as a h...

Background and objective: Pterygota alata (Roxb.) R.Br. bark extract has been studied to have cytotoxic activity on 4T1 cells. This study was conducted to determine the cytotoxic activity of several fractions of Pterygota alata (Roxb.) R.Br. bark against 4T1 breast cancer cells and to investigate the most active fractions on Bcl-2 and Bax expressi...

Coronavirus disease (COVID-19) is a global pandemic in the world. Some treatments, including vaccines and potential drugs, are still developed. This study investigated the bioactive compounds of Zingiber officinale, Kaempferia rotunda, and Curcuma zedoaria as a potential inhibitor for ACE2 and RdRP proteins. Molecular docking was used for screening...

Classification of active compounds is necessary to trivialize their properties and activities and can be carried out by identifying functional groups and their structures based on the simplified molecular input line entry system (SMILES) code. This study classified active compounds based on the SMILES structure using a combination of the machine le...

Vascular endothelial cells have a variety of functions such as the control of blood coagulation, vascular permeability, and tone regulation, as well as quiesce of immune cells. Endothelial dysfunction is a cardiovascular events predictor, which is considered the initial stage in atherosclerosis development. It is characterized by alterations in end...

The Sterculiaceae family is known to have many benefits in the fields of food, medicine and industry. The S. quadrifida R.Br and S. foetida Linn. is a plant of the family Sterculiaceae which has utilization in the field of medicine. Empirically used to stamina booster, cure diarrhea, treat hepatitis, relieve infections and anti-cancer. In the resea...

The active compound could interact with other molecules and can lead to a variety of positive and negative effects on the living system. Therefore, classification of the compound is very important for understanding its character and functions for human medicine. The manual classification of compound functions by laboratory test is time and cost con...

Background: Elephantopus scaber (ES) and Sauropus androgynous (SA) have been frequently reported to possess antibacterial activity through in vitro, but in vivo studies about the protective effect of combined ES and SA have acquired less attention. Objectives: To evaluate protective effect of combined ethanol extract of ES and SA on hormone imba...

Zika virus (ZIKV) is a member of the Flaviviridae family. This virus is spread and carried by mosquitoes (Aedes aegypti and Aedes albopictus). ZIKV belongs to the Flavivirus genus, enveloped, and positive-sense RNA that encodes structural (E) and non-structural (NS) proteins. the envelope glycoprotein of ZIKV interact with the Axl receptor to fuse...

It has been known that the immunoglobulin levels were altered in diabetes mellitus (DM) conditions. This study aimed to evaluate the levels of immunoglobulins in DM mice after the administration of Moringa oleifera-Ifalmin® formulation (MI). Streptozotocin, at a dose of 145 mg.kg-1, was injected intraperitoneally to experimental mice to obtain diab...

Background Hepatocellular carcinoma (HCC) is a serious disease and is the third main cause of death in the world. Hepatitis B virus infection can lead to HCC. The virus introduces genetic material into the host, damages DNA, and interferes with the activity of the apoptotic and tumor suppressors to trigger the formation of an oncogene. However, mos...

Background Ready to eat (RTE) vegetables are easily accessible healthy foods that are commonly consumed globally, including in Indonesia. However, these RTE vegetables contain potential contamination from pathogens and multi-drug resistant bacteria. Therefore, in the present study, we examined the presence of tetracycline-resistant E. coli (TRE) is...