Nashi Widodo

• University of Brawijaya

Context: Sea cucumbers have been identified as highly potential contenders in the fight against breast cancer. Aims: To investigate the potential of Holothuria scabra extract for anticancer against breast cancer cells in vitro by investigating its synergistic effects when integrated with frequently used chemotherapeutic medications. Methods: The Co...

Free radicals can cause oxidative damage if they are produced in excess amounts in the body. To prevent this, the work of endogenous antioxidants needs to be supported by safe and effective exogenous antioxidants. C. nutans is one of the sources of natural polyphenol compounds that have the potential to be used as free radical-scavenging agents. Th...

Leptastrea purpureais a species of hard coral with potential bioactive properties. This study aimed to evaluate the antioxidant properties of the n-hexane extract of L. purpureain vitroand in silico. The in vitroantioxidants activity was assessed by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, and ferric reducing antioxidant power (...

Objectives: Hypertension, commonly known as high blood pressure, is a prevalent and serious condition affecting a significant portion of the adult population globally. It is a chronic medical issue that, if left unaddressed, can lead to severe health complications, including kidney problems, heart disease, and stroke. This study aims to develop a f...

Breast cancer remains a major public health challenge, highlighting the importance of finding new therapeutic approaches, including the use of traditional plants with unstudied medicinal properties. This study aims to explore the bioactive potential of Tinospora cordifolia stem extract by investigating the content of flavonoids, phenols, terpenoids...

Excessive inflammation, driven by macrophage activation and nitric oxide (NO) production, underlies various diseases. This study investigates the anti-inflammatory potential of bungur (Lagerstroemia speciosa L.) leaf extract. We characterized its phytochemical profile and evaluated the effects using in silico approaches. Bungur leaves (L. speciosa...

Blumea balsamifera (Bb) is a plant used as herbal medicine in Southeast Asia, and it has been used due to its antibacterial, antiinflammatory, anticancer, etc. However, there is currently limited evidence that Bb leaf extract from Batu, Indonesia, contains beneficial compounds against breast cancer. Hence, this study evaluates the active compounds...

Obesity, which is characterized by excessive body fat accumulation and energy imbalance, is a major risk factor for type 2 diabetes mellitus. Boesenbergia rotunda rhizomes (known as fingerroots) exhibit a variety of pharmacological activities and are used in traditional medicine. Pinostrobin was identified as a major constituent of Boesenbergia rot...

Obesity is characterized by abnormal fat accumulation in the white adipose tissue (WAT), posing a significant health risk. Enhancing energy expenditure through WAT browning using dietary components and natural extracts is a promising therapeutic strategy for obesity management. This study explored the efficacy of citral isomers from Cymbopogon citr...

Background: Lactococcus lactis is a promising oral vaccine carrier. However, to improve the stability of the bacteria, the freeze-drying method and formulation need to be optimised. Objective: To develop a formulation of oral recombinant food-grade Lactococcus lactis as a candidate oral vaccine for coronavirus disease 2019 (COVID-19). Method: To pr...

Background Antimalarial resistance in Indonesia is caused by mutations at the drug target sites and biochemical changes in drug receptors. Previous research indicates that only fraction 49 of Streptomyces hygroscopicus Subsp. Hygroscopicus (S. hygroscopicus) is effective as an antimalarial agent in in vitro tests. Methods The active compounds of se...

Metabolic syndrome (MetS) is a group of risk factors in the form of central obesity, insulin resistance, dyslipidemia, and hypertension, increasing oxidative stress. This pathological event leads to the development of cardiovascular disease, for instance, atherosclerosis. Besides modifiable risk factors, non-modifiable risk factors such as genetic...

Breast cancer is a type of cancer that has a high rate of diagnosis and mortality in the world. Lagerestroemia speciosa is a herb that has anticancer activity. This study aims to analyze the compounds in L. speciosa that most act as anticancer of the breast. The compounds in L. speciosa were selected based on drug-likeness, physicochemical properti...

This study aimed to develop an epitope-based vaccine of SARS-CoV-2 S protein through an immuno-informatics study. The whole genome of SARS-CoV-2 sequences was obtained from the GISAID database and then trimmed to obtain the S protein sequences. The alignment was done by Clustal-W of MEGA software. Epitope prediction and modeling were performed by D...

In recent years, the search for new anticancer drugs from local plants in Indonesia has become an interesting topic for research. One of the typical Indonesian local plants that have been used by the community as an anticancer traditional medicine (jamu) is Spatholobus littoralis Hassk (SLH) or better known as Bajakah. The native Dayak people of Ka...

Aim To evaluate the essential role of Moringa-albumin (MA) formulation in the maintenance of complement regulatory proteins through CD55 and CD59 on erythrocyte (TER-119). Methods Strepto-zotocin (145 mg/kg) was used to induce diabetes mice. In diabetes mellitus (DM) model mice were treated with MA formulation for 14 days at a D1 - 500 mg/kg (M) +...

Luminal A is the most widely diagnosed breast cancer subtype worldwide. The existing luminal A therapy faces some challenges, such as drug resistance and toxicity. Therefore, new therapeutic approaches are urgently required. The application of medicinal plants, particularly aerial parts of Selaginella doederleinii Hieron, becomes an attempting stra...

COVID-19 is a pandemic that has an impact on all aspects of life. This pandemic is caused by the SARS-CoV-2 virus, which has a fast pathogenesis cycle, so alternatives are needed, such as incredibly natural and marine ingredients that can be used as drug candidates. Holothuria atra is an animal candidate that has great potential. This study aims to...

Breast cancer remains a significant challenge in oncology, necessitating the exploration of novel therapeutic options. This study investigates the anticancer effects of ethanol and n-hexane fractions derived from the methanol extract of the Leptastrea purpurea sponge on T47D breast cancer cells. Employing cytotoxicity assays (MTT and WST-1), mitoch...

Hydrogels are a 3D polymer network capable of absorbing large amounts of water or biological fluids without dissolving, due to chemical or physical crosslinking. They are made from cellulose derivatives and are a promising research area for developing smart drug carriers in modern drug delivery systems. The present research aimed to synthesize h...

Excessive production of nitric oxide (NO) dan pro-inflammatory cytokines can lead to tissue damage and chronic inflammation. Euphorbia hirta presents an interesting opportunity for plant-derived anti-inflammatory agents as it contains a range of compounds which have demonstrated anti-inflammatory effects. This study investigates the anti-inflammato...

Seagrass beds are essential components of coastal communities globally, as they support productive fisheries and coastline stabilization. In view of its numerous ecosystem services, the coverage of seagrass is an important indicator of ecosystem health. However, there are conflicting data regarding Indonesia’s seagrass coverage. Some research indic...

Dugong dugon, an endangered marine mammal is listed the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES). Dugongs are classified as herbivorous mammals that live in the shallows associated with seagrass beds. The objectives of this study are (1) to collect data on dugong encounters in East Java, both direct en...

Myriad studies have shown that sea cucumber is a promising anti-inflammatory and anticancer agent. Therefore, a study to identify the active compounds contained in sea cucumber, which have the potential as nuclear factor kappa-light-chain-enhancer of activated B cell (NFκB) inhibitors was conducted. NFκB plays a significant role in regulating infla...

Kraits (Bungarus spp.) are the snakes that commonly cause fatal bites in Tropical Asia due to their neurotoxic venom. The complex process of antivenom production contributes to the lack of available antivenom and amplifies the burden of snakebite in that region. Thus, the victims seek traditional medicine such as root extract from nut grass (Cyperu...

div align="center"> Recent healthcare research has focused a great deal of interest on using genetic data analysis to predict the risk of hypertension. This paper presents a unique method for accurately predicting the vulnerability to hypertension by utilizing single nucleotide polymorphism (SNP) data. We present a novel neural network design utili...

Treatment options for diabetes may vary widely, however, around 50% of all diabetic patients do not reach the minimum glycemic target. Therefore, drugs that are able to lower glucose levels through unconventional targets are still needed for the treatment of type 2 diabetes. This study aimed to investigate the activity of cardiotoxins from N. sputa...

Malang merupakan salah satu kota pelajar yang memiliki berbagai sekolah dan perguruan tinggi yang terpercaya bagi pelajar dari berbagai daerah untuk mendapatkan pendidikan yang berkualitas. Pembelajaran di tingkat SMA bukan hanya terfokus pada kegiatan belajar mengajar, namun juga pada keterampilan laboratorium yang dapat meningkatkan kemampuan sis...

Caesalpinia sappan, commonly known as secang in Indonesia, has been used in traditional medicine to treat inflammation, microbial infection, diabetes, and cancer. In this study, the chromatographic separation of the ethyl acetate fraction of C. sappan was carried out. It resulted in the isolation of 2′,5,6-trimethoxyflavone (TM) and cirsimaritin (C...

The collagen source could be derived from fish scales like snakehead fish (Channa striata). One of the possible methods can be used enzymatic methods. The objective of this research was to isolate and characterize the collagen from C. striata scale using B. subtilis protease. This study was an experimental method utilizing the protease enzyme produ...

Buku ini menawarkan wawasan mendalam ke dalam dunia kimia komputasi dan aplikasi notasi SMILES. Penulis memandu pembaca melalui perjalanan yang komprehensif, dimulai dari pemahaman dasar notasi SMILES hingga teknik mendekode notasi ini. Selain itu, buku ini menguraikan bagaimana Machine Learning, khususnya pendekatan Extreme Learning Machine (ELM)...

Context: The development of functional drinks to inhibit oxidative stress and inflammation as a critical process in inducing heart damage in metabolic syndrome is required. Coffee, tea, and turmeric have all been shown to offer health advantages. Aims: To investigate the effect of coffee, green tea, turmeric extract (ECGTT) against cardiac-metaboli...

Background : Insulin resistance has been independently associated with cardiac diseases. Free fatty acids are recently known to induce cardiac insulin resistance due to low-grade inflammation. Therefore, the improvement of free fatty acid levels can also improve cardiac insulin resistance. This study investigated the combination of green tea and de...

Objective: This study will identify specific epitopes from the 47kDa protein as the basis for making polyclonal antibodies to increasing sensitivity and specificity of 47kDa protein as bladder cancer biomarkers. Method: The 47kDa protein epitope prediction was carried out using the in-silico method. The epitope with the highest and the lowest va...

Marine echinoderms, specifically the sea urchin species Diadema setosum , may hold promise in the medical field as potential sources of antiviral agents for Covid-19. Recent research focused on identifying the compounds within their extracts and their effectiveness as inhibitors for the 3CL pro , RdRp, and ACE2 proteins through in silico modelling....

Previous investigation discovered a phenylpropanoid compound called (2E,4E)-1,5- diphenylpenta-2,4-dien-1-one in the chloroform fraction of Sterculia quadrifida seeds. This compound exhibited notable cytotoxic effects, displaying IC50 values of 2.29, 9.93, 18.09, and 12.12 μg/mL in 4T1, MCF-7, MDA-MB-435, and T47D breast cancer cell lines respectiv...

The purpose of this study is to determine the effect of the Religiosity, Hardworking on performance of Islamic microfinance or Baitul Maal wat Tamwil (BMT), Indonesia. BMT is a microfinance institution that operates based on sharia principles and is a legal entity. We distributed questionnaires to 52 employee respondents at BMT Ummat Sejahtera Abad...

Sterculia quadrifida has been empirically used as a traditional plant for medicinal purposes. The objective of this research is to isolate secondary metabolites present in the seeds of S. quadrifida. Extracts of S. quadrifida seeds were prepared using 80% methanol, and then separated into various fractions using liquid-liquid partition with solvent...

Laksono RM, Kalim H, Rohman MS, Widodo N, Ahmad MR, Halim W. J Pain Res. 2023;16:1697–1711. The authors have advised affiliation 1 on page 1697 is incorrect. The text “Doctoral Program in Biomedical Science” should read “Doctoral Program in Medical Science”. The authors apologize for this error.

Background Cardiovascular diseases (CVD) are the primary medical manifestation of metabolic syndrome (MetS). Hypoxia is also involved in the pathogenesis of CVD. Since dietary intervention significantly improved the physiological condition in MetS, the development of functional food to complement conventional medical therapy is essential. Among sev...

Background The molecular mechanism of pulsed radiofrequency (PRF) in chronic pain management is not fully understood. Chronic pain involves the activation of specific N-Methyl D-Aspartate receptors (NMDAR) to induce central sensitization. This study aims to determine the effect of PRF on central sensitization biomarker phosphorylated extracellular...

The COVID-19 pandemic began at the end of 2019 in Wuhan, China, and has spread throughout the world. Vaccination is still the most effective method of prevention of pathogenic infections, including viral infections. However, there is little evidence that vaccination can protect against SARS-CoV-2 virus for a long time. Thus, regular re-vaccination...

Context: Aerobic glycolysis is a breast cancer hallmark that has become a novel target for anticancer therapy. GLUT1, HK2, PKM2, and LDHA are oncogenic proteins in the pathway. The compounds of ethyl acetate fraction of Selaginella doederleinii Hieron extract can be developed as aerobic glycolysis inhibitors by impeding the proteins’ activity. Aim...

Oxidative stress and inflammation have a role in the development of various diseases. Oxidative stress and inflammation are associated with many proteins, including Kelch ECH associating protein 1 (KEAP1) and inducible nitric oxide synthase (iNOS) proteins. The active compounds contained in Holothuria scabra have antioxidant and anti-inflammatory p...

Synthesis and characterization of cellulose acetate and nanocellulose acetate have been carried out. Cellulose was isolated from sengon wood powder waste using 6% NaOH solution and bleached with NaOCl. The cellulose was hydrolyzed with 60% formic acid to obtain nanocellulose. Cellulose and nanocellulose were then modified with acetic anhydride. The...

nSARS-CoV2 causes pneumonia and acute respiratory distress syndrome that involves exacerbated pro-inflammatory cytokines hyperproduction. ZICURMA, zing-curcuma is a tonic developed from ginger, turmeric, and curcuma that may be adequate to remedy nSARS-CoV2 infections. Therefore, this study aims to predict the effectiveness and possible inhibition...

The hypocholesterolemic activity of soy isoflavones has been studied, but the exact mechanism underlying the activity remains unclear. This study reveals the proposed mechanism of the cholesterol-lowering effect of soy isoflavones by computational simulations. Daidzin, Glycitin, Genistin, Daidzein, Glycitein, Genistein, Glyceollin I, Glyceollin II,...

In our previous study, aurone compound were found in the n-butanol fraction of Sterculia quadrifida leaves. The aurone compound (compound 1) had high cytotoxic activity in cell lines with IC50 values of 4.05, 12.53, 15.38, and 25.91 μg/mL in 4T1, MCF7, MDA-MB-435, T47D, respectively. In this study, we performed the MMP-9 enzyme inhibition test with...

Solving health problems is part of the enigma in finding candidate solutions, one of which is drugs for certain diseases, especially Covid-19, which include those the extensive and complete research of which involves the Human Genome, viruses, and medicinal compounds. Some of the obstacles are the limited knowledge as an approach that to date tends...

This study investigates the synthesis, chemical, and physical properties of isothiocyanate poly(methyl eugenol) or IPME and thiosemicarbazide poly(methyl eugenol) or TPME. The IPME synthesis as a precursor was firstly carried out by reacting Methyl Eugenol (ME) with potassium hydrogen sulfate and potassium thiocyanate in chloroform solution at room...

Context: Breast cancer most commonly occurs in women globally and has the highest mortality rate in Asia. Therefore, a safe and prominent drug to cure the disease needs to be urgently developed. Aims: To investigate the molecular mechanism of the ethanol extract of Curcuma xanthorrhiza (ECx) ininducing apoptosis in breast cancer cell line T47D. M...

Dietary intervention plays a significant role in regulating hyperlipidemia. Besides, elicited soybean has greater bioactive contents with numerous health benefits potential. However, there is no evidence of the hypolipidemic activity of elicited soybean. This study will explore the potential mechanism of hypolipidemic activity of bioactive compound...

Echinoderms are marine water invertebrates that are showing diverse morphologies and grouped in five classes, however the reports on this species from Gili Ketapang Island, East Java is currently limited. DNA barcodes (DNA fragments of the 5' end of the cytochrome c oxidase subunit I gene, COI) have been used to support the morphological examinatio...

Background: Elephantopus scaber and Phaleria macrocarpa have recently been interested as novel anticancer agents. However, there was no scientific evaluation of the anticancer effect of both plant combinations. Objective: This study investigated the potential anticancer effects of combined E. scaber and P. macrocarpa leaves extract on human brea...

Tyrosine kinase inhibitors (TKIs) are widely used in cancer treatment due to their effectiveness in cancer cell killing. However, an off-target of this agent limits its success. Cardiotoxicity-associated TKIs have been widely reported. Tyrosine kinase is involved in many regulatory processes in a cell, and it is involved in cancer formation. Recent...

Breast cancer is a malignant cancer with annually increasing incidence and mortality rates. The anticancer effect of Curcuma zedoaria (Berg.) Roscoe ethanol extract (ECz) on T47D breast cancer cells was investigated in vitro. The ECz contains phenolic and flavonoid compounds that may play a critical role in anticancer activity. The viability of the...

Background: Turmeric ( Curcuma longa ) has high potential as a traditional anticancer drug. This study aimed to analyze the anticancer activity of turmeric ethanol extract on T47D cells and examine the interaction of Akt1 protein with compounds contained in turmeric. Methods : The cytotoxicity assay was conducted using WST-1 reagents. Apoptosis ass...

Breast cancer is the most common type of cancer women suffer from worldwide in 2020 and the 4th leading cause of cancer death. Boesenbergia rotunda is an herb with high potential as an anticancer agent. This study explores the potential bioactive compounds in B. rotunda as anti-breast cancer agents using in silico and in vitro approaches. The in si...

Multidrug treatment has been piloted for Plasmodium falciparum malaria infection; however, multidrug resistance requires serious attention. Therefore, new antimalarial studies have been intensively carried out, including research on new compounds containing nitrogen and sulfur atoms that are predicted as active groups against antiplasmodial. This s...

Plasmodium falciparum malaria contributes to significant global diseases. Computer-aided drug design, screening, and discovery were used to analyze a novel series of Methyl Eugenol Benzaldehyde Thiosemicarbazone (MEBThi) and Methyl Eugenol Cinnamaldehyde Thiosemicarbazone (MECThi) derivatives for malaria falciparum inhibition. This present study sh...

This study aims to determine the various kinds of compounds from the saponin group found in local Indonesian garlic and map the biological potential of these compounds, especially the potential in the health sector. Saponins are a group of compounds characterized by the presence of foam. Saponins have various benefits both in the health, agricultur...

Background: The standardization and mechanism of action of Caesalpinia sappan as an anticancer agent are still lacking. This study aimed to understand the mechanism of action of C,sappan extract as an anticancer agent. Methods: This study was conducted using the A549 lung cancer cell line to understand the mechanism of action of C. sappan extract a...

Purpose: This study aims to increase and direct the capital owned by SMEs so that they are able to survive in crisis conditions. Theoretical framework: During the covid-19 crisis, companies sought innovative ways to survive. They must utilize inter-business networks, select productive activities, and adjust their marketing strategies. Agents of art...

Context Coffee and green tea extract combination is expected to reduce macrophage migration. Aims We investigated the effects of combination of coffee and green tea extracts on GALNT2 gene expression and PSGL-1 protein expression in Ox-LDL-induced RAW 264.7 cells. Materials and methods RAW 264.7 cells were treated with a combination of coffee and...

Serine racemase (SR) catalyzes L-serine racemization to activate the N-methyl-D-aspartate receptor (NMDAR). NMDAR activation is associated with the progression of acute-to-chronic neuropathic pain. This study aimed to investigate NMDAR antagonist interactions with SR to obtain potential chronic pain target therapy. Several NMDAR antagonist drugs we...

The purpose of the current study is to review ethno-medicinal plant used by natives in West Kalimantan Province in last five years. The methods used is gathering earlier publications in journals completed with pharmacological evidence of local medicinal plants. The present review result reported that 346 specieses belonging to 95 families have been...

The purpose of the current study is to review ethno-medicinal plant used by natives in West Kalimantan Province in last five years. The methods used is gathering earlier publications in journals completed with pharmacological evidence of local medicinal plants. The present review result reported that 346 specieses belonging to 95 families have been...

Phaleria macrocarpa is a medicinal plant widely used in Indonesian folk medicine to treat several diseases, including cancer. However, the comparative evaluation of various plant parts of P. macrocarpa has not been studied for their anticancer properties on breast cancer. The study aimed to assess the antiproliferative activity of the ethanol extra...

Spatholobus littoralis Hassk has biological activities such as strong antioxidants, booster of leydig cell enhancer and testosterone level booster in male rattus norvegicus, wound healer on the white mouse incision and cytotoxic against cell culture MCF7, HepG2, T47D, WIDR, HTB, and HeLa. This present study aim is to predict predict bioactive compo...

Severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2), the causative agent of coronavirus disease-19 (COVID-19), has become a global health issue. Spike proteins from the virus have a vital role in infection. Herbal medicines such as Lagerstroemia, Euphorbia hirta, and Kleinhovia hospita have several pharmacological functions such as antican...

Cancer is a major health problem worldwide and the leading cause of death in many countries. It remains challenging to find anticancer treatments that work efficiently for varying types of cancer cells. Several studies revealed that nuclear factor kappa B (NF-κB) is a family of dimeric transcription factors that induce tumor promotion, progression,...

Ragi Tape (RT) is commonly used as the microbial starter for various Indonesian traditional food. Consist of diverse microbial populations, RT has excellent agents as elicitors to augment the bioactive compounds in Soybean (SB) and Mungbean (MB). Metabarcoding analysis has shown to identify the microbial population involved in elicitation using RT....