Naser Harzandi

• PhD
• Islamic Azad University, Karaj

Background and Objectives: Leptospirosis is an infectious zoonotic disease that can result in severe complications. It is widespread, especially in hot and humid climates such as the northern region of Iran. The immune responses to leptospirosis are multifaceted. Lipl41 is an outer membrane protein that is expressed during infection and is highly c...

Background: Drug resistance is currently possible anywhere in the world. Due to the discovery of antimicrobials, medicine, and health have made tremendous advances over the past several decades. Aim: This research evaluated the antimicrobial and cytotoxicity effects of green synthesis of copper oxide nanoparticles (CuO NPs) on Lactobacillus acid...

The propolis produced by bees is used by them to protect their hives. The cavity inside the hive’s walls is filled in during cold days to reduce entry points and mummify any intruders to ensure their survival. A current focus in nanotechnology and nanoscience is the green biosynthesis of nanoparticles (NPs) using biomaterials. Research on green met...

Background: Echium amoenum (E. amoenum), as one of the most popular plants in Iran, is traditionally used to treat different types of disorders. Objectives: This experimental study aimed to evaluate the modulatory effects of E. amoenum on permethrin (PMN)-induced oxidative stress in rats and to determine the cytoprotective effect of E. amoenum on P...

Saffold virus as a newly discovered virus, which seems to be related to acute gastroenteritis as with other enteric viruses and to human airway diseases in children belongs to Cardiovirus genus in picornaviridae family with 11 genotypes. Saffold virus initially was detected in America from infant stool sample. Saffold virus has also been detected i...

Background: Dental caries is considered as one of the most significant global health problem over the world. Dental caries initiates from bacterial shifts within the supragingival biofilm, then a polymicrobial biofilm is formed on the surface of tooth, and finally various bacterial species aggregate in a complex-organized manner. The exploiting va...

Nanosilver and nanocurcumin are popular nonmaterial with increasing public attention, but their synergistic therapeutic potentials in burns have never been considered. The present study aimed to provide a novel formulation from both nanomaterials (Ag-Curcumin NPs) and conduct their preclinical evaluation for burn healing. After evaluation of partic...

Silver nanoparticles (AgNPs) are one of the most important nanoparticles which have various biomedical applications. For example, as antifungal, antibacterial, anti-cancer and anti-inflammatory agents. Skin infection caused by Trichophyton rubrum and some opportunistic fungi such as Candida albicans and Aspergillus fumigatus are sometimes difficult...

Background & objectives: Immunosuppressive drugs that are used for decreasing risk of acute rejection and renal graft loss can lead to reactivation of latent viruses for example BKV and JCV in either renal allograft or recipients. These viruses can lead to renal graft loss. The purpose of this study was to evaluate the quality and quantity of the g...

The need for an intensive care protocol, sometimes weekly or biweekly, has led to a significant increase in laboratory costs for kidney recipients. In the present study, an inhouse tetraplex nested PCR assay was developed and validated for the specific detection of BKV, JCV, HCMV and EBV in clinical samples. We determined the Limit of Detection (LO...

Newcastle disease virus (NDV) is considered as a deadly infection to majority of the avian species. One of the key factors responsible for virus pathogenesis is sialidase protein expressed by hemagglutinin-neuraminidase gene on the envelope of virus particles, which involves in virus attachment to cells. In this study we compared restriction map of...

Background Increasing the expression rate of recombinant mammalian hormones in Escherichia coli by combining efficient promoters and signal sequences is a never ending process. A self-induced promoter will have some beneficial gains compared to the classical T7 promoter or its variants with isopropyl β-D-1-thiogalactopyranoside (IPTG) as the induce...

Biofilm associated Pseudomonas aeruginosa infections are of major clinical concern due to treatment failure by conventional antibiotics. Referring to many reports, antimicrobial peptides (AMPs) would be act as a new promising agent to overcome the issue. In this regard, our study was aimed to evaluate the kinetics of melittin as a natural AMP, in s...

Background: Non-typhoidal salmonellae and serovars of Escherichia coli, Listeria, Shigella, and Campylobacter are among the most important food-borne bacterial agents. Objective: The aim of the present study was molecular detection of quinolone resistance in Salmonella spp. isolated from poultry products in Karaj, Iran, using polymerase chain react...

Hemagglutinin–neuraminidase (HN) protein besides its mediation in viral pathogenesis, is composed of various antigenic sites which stimulate production of host’s antibodies. Thus, application of this protein in serological tests and vaccination plays a major role in biosecurity and control programs. In the present study, we designed a recombinant H...

Background: Saffold virus as a new member of cardiovirus genus in picornaviridae family has been suggested to be related to diarrheic cases and human airway diseases. However, relationship between Saffold virus and human diseases is unclear. In order to establish an investigation for the occurrence of Saffold virus among pediatric patients involved...

مقدمه: استفاده از القاگرهای سمی، مانند Isopropyl β-D-1-thiogalactopyranoside (IPTG)، در توليد زيست‌داروها بر اساس رهنمودهای سازمان‌های نظارتی ممنوع می‌باشد. از این رو، مطالعه‌ی حاضر با هدف ارزیابی و بررسی امکان استفاده از سيستم‌های بيانی خود القايی برای توليد پپتيد YY(3-36) به عنوان يک ماده‌ی اوليه‌ی دارويی (Active pharmaceutical ingredient یا API)...

Introduction Salmonella is known as one of the most important causes of gastrointestinal disease in the world. Quinolones and fluoroquinolones are used successfully in the treatment of salmonellosis particularly for infections that have become resistant to several antibiotics. But non-susceptible isolates to quinolones have been reported in several...

Nontuberculous mycobacteria (NTM) have emerged as an important cause of opportunistic nosocomial infections. NTM has frequently been isolated from hospital water distribution systems. The aim of this study was to survey the risk of NTM infections and determine the prevalence of NTM species in the hospital water distribution systems in Tabriz, Iran....

Water is one of the important reservoirs of environmental mycobacteria (EM). The resistance against chlorine and biofilm formation is among the important factors involved in colonization of these bacteria in water. Environmental mycobacteria cause different types of diseases especially pulmonary diseases due to their transfer to humans. The isolati...

Phylogenetic relationship study and polymorphism analysis of novel NDV outbreak in Iran, which reveals emergence of new sub-genotype in genotype VII cluster based on HN coding sequence.

Background: Escherichia coli is recognized as a major food-borne pathogen in humans worldwide. Antibiotic resistance of Escherichia coli isolates is a concern that has created widespread problem for treatment of infections caused by this bacterium. The aim of this study was to determine the pattern of aminoglycoside resistance among the strains of...

Background: Campylobacter spp. is recognized as one of the important bacterial agents of food borne diseases worldwide. C. jejuni and C. coli are the most frequently reported species causing bacterial gastrointestinal disorders in developed and some of the developing countries. Objectives: The goals of this survey were to detect and to differentiat...

Methicillin-resistant S. aureus(MRSA) is one of the most important pathogens that cause a wide range of hospital and community-acquired infections worldwide. In the present study, the Multiplex PCR test was employed to detect the genes 16S rRNA (Staphylococcus genus specific), femA (encoding a factor essential for methicillin resistance), mecA (enc...

Microorganisms can be used in order to break down and clean any type of a specific class of compounds which are found in petroleum and its related products. In this study, the rate of biodegradation of petroleum hydrocarbons by five isolated cyanobacteria from oil polluted region of south part of Iran has been studied. These include Anabaena ISC55...

Clinical significance and epidemic outbreaks of multi-drug resistant Acinetobacter baumannii (MDR-AB) in intensive care units (ICUs) are increasing. We provided a molecular epidemiology research of 85 A. baumannii isolates of southern Iran using amplified restriction fragment polymorphism (AFLP) DNA fingerprinting method. Using a cut-off of 90% sim...

Background and purpose: Misabortion is a disorder resultant of numerous causes such as infections. It is clear that bacterial infections due to genital mycoplasma, Listeria and brucella can cause septic abortion. A right time diagnosis of these infections can improve women fertility. This study was conducted to survey the efficiency of PCR in detec...

Background: Introduction of HAART (highly active anti-retroviral therapy) has greatly increased the survival time of HIV/AIDS patients in the word. However, the occurrence of drug-resistant strains requires new anti-retroviral drugs, one of which, an integrase inhibitor (INI), has been recently approved and used for therapy in many countries. Soone...

The incidence of methicillin resistance has risen among nosocomial isolates of Staphylococcus aureus and coagulase negative staphylococci. The present study was carried out to investigate the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant coagulase negative staphylococci (MRCoNS) and to determine their an...