Naoufel Haddour

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Verified

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• PhD
• Professor (Associate) at École Centrale de Lyon

Plant microbial fuel cells (PMFCs) are electrochemical systems capable of producing electrical energy from plant growth. Despite their limited power density, PMFCs hold pomise as power source for wireless sensor, without using embedded battery, especially in isolated locations. In this study, a complete wireless system was presented, with Cyperus p...

This investigation examined the role of shear stress on the dynamic development of microbial communities within anodic biofilms in single-chamber microbial fuel cells (MFCs). Bacterial attachment to surfaces, often regarded as a crucial step in biofilm formation, may significantly contribute to the selection of electroactive bacteria (EAB). It is w...

This research sought to enhance the efficiency and biocompatibility of anodes in bioelectrochemical systems (BESs) such as microbial fuel cells (MFCs), with an aim toward large-scale, real-world applications. The study focused on the effects of acid-heat treatment and chemical modification of three-dimensional porous pristine carbon felt (CF) on po...

MFCs represent a promising sustainable biotechnology that enables the direct conversion of organic matter from wastewater into electricity using bacterial biofilms as biocatalysts. A crucial aspect of MFCs is how electroactive bacteria (EAB) behave and their associated mechanisms during extracellular electron transfer to the anode. A critical phase...

This study has provided comprehensive insights into the intricate relationship between shear stress and the development, structure, and functionality of electroactive biofilms in Microbial Fuel Cells (MFCs). A multichannel microfluidic MFC reactors that created specific shear stress on the anode, were designed for the simultaneous study of multiple...

This study introduces the utilization of self-powered microbial fuel cell (MFC)-based biosensors for the detection of biotoxicity in wastewater. Current MFC-based biosensors lack specificity in distinguishing between different pollutants. To address this limitation, a novel approach is introduced, capitalizing on the adaptive capabilities of anodic...

Off-grid electrical energy systems based on renewable energy sources (RESs) have become increasingly popular for their ability to generate low-carbon electricity in remote areas without access to traditional power grids. These systems rely on the effective management of RESs and storage solutions. Designing and sizing these systems can be a complex...

In recent years, there have been advancements in the development of bacterial electrochemical sensors for toxicity monitoring, especially through utilization of electroactive bacteria. Accordingly, this mini review summarizes the recent advances in the design of bacterial‐based electrochemical sensors with a specific discussion of main methodologie...

In this study, the relationship between pyrolysis temperature of woody biomass and physicochemical properties of derived biochar was investigated for microbial fuel cell (MFC) application. Physical and chemical properties of biochar were characterized for different pyrolysis temperatures. Results showed that biochar obtained at 400 °C was not condu...

Herein, the authors propose a miniaturized glucose/O2 n-EFC based on a new direct electron transfer. The anode is a screen-printed carbon electrode (SPCE) modified with functionalized carbon nanotubes (f-CNTs) and cauliflower-like PdAu nanostructures (PdAuNS). The PdAuNS/f-CNT biomimetic nanocatalyst was prepared using a cost-effective and straight...

In this work, we propose a novel functionalized carbon nanotube (f-CNT) supporting nanoporous cauliflower-like Pd nanostructures (PdNS) as an enzyme-free interface for glucose electrooxidation reaction (GOR) in a neutral medium (pH 7.4). The novelty resides in preparing the PdNS/f-CNT biomimetic nanocatalyst using a cost-effective and straightforwa...

Low electrical conductivity of carbon materials is a source of potential loss for large carbonaceous electrode surfaces of MFCs due to the long distance traveled by electrons to the collector. In this paper, different configurations of titanium current collectors were used to connect large surfaces of carbon cloth anodes. The current collectors had...

This study investigated the effect of external resistance (Rext) on the dynamic evolution of microbial communities in anodic biofilms of single-chamber microbial fuel cells fueled with acetate and inoculated with municipal wastewater. Anodic biofilms developed under different Rext (0, 330 and 1000 ohms, and open circuit condition) were characterize...

Plant Microbial Fuel Cell (PMFC) creates electricity from oxidation of root exudates by microbia anaerobic digestion, and reduction of dioxygen to water. In this study, Lobelia Queen Cardinalis was used as a plant model to investigate the impact of ionic connection between stacked Plant microbial fuel cell (shared soil). 10mm thickness carbon felt...

The development of high-performance anode materials is one of the greatest challenges for the practical implementation of Microbial Fuel Cell (MFC) technology. Copper (Cu) has a much higher electrical conductivity than carbon-based materials usually used as anodes in MFCs. However , it is an unsuitable anode material, in raw state, for MFC applicat...

Les usines de traitement des eaux usées consomment de grandes quantités d'énergie qui sont principalement achetées sur le réseau, ceci alors même que les eaux usées contiennent plus d’énergie sous forme chimique que nécessaire à leur propre traitement. Le potentiel énergétique des eaux usées peut être judicieusement exploité par de nouvelles techno...

In this paper, the degradation of the diazo dye naphthol blue black (NBB) using the Galvano-Fenton process is studied experimentally and numerically. The simulations are carried out based on the anodic, cathodic, and 34 elementary reactions evolving in the electrolyte, in addition to the oxidative attack of NBB by HO• at a constant rate of 3.35×107...

The present paper investigates the potential of the Galvano-Fenton process as an advanced technique in terms of the simultaneous oxidation of a model pollutant, phenol, and the energy release and saving as compared to conventional electrochemical techniques, namely, Fenton, Fenton-like, and Electro-Fenton. A numerical model describing the electroch...

A novel approach allowing the production of electrical energy by an advanced oxidation process is proposed to eliminate organic micropollutants (MPs) in wastewaters. This approach is based on associating the Galvano–Fenton process to the generation of electrical power. In the previous studies describing the Galvano–Fenton (GF) process, iron was dir...

In the present study, the kinetics of degradation of acid orange 7 (AO7) by the Galvano-Fenton process is assessed experimentally and simulated numerically in order to figure out the mechanism of generation and consumption of free radicals with this novel advanced oxidation process. 10 mg/L of the azo dye are degraded during 20 min by combining a c...

Sensors based on voltammetry and amperometry are involved in several fields such as clinical, food, environmental, pharmaceutical, and industrial sectors. The present article describes the principle of the voltammetry and amperometry and highlights the practical applications of various analytical techniques such as cyclic voltammetry, differential...

The present paper constitutes a conjugate numerical and experimental study of a novel process patented as Galvano-Fenton, based on the association of galvanic corrosion and Fenton reaction. The study investigates the pathways of the in-situ formation and regeneration of iron species involved in electrochemical and chemical mechanisms of the Galvano...

Modification of electrode surfaces is a promising strategy to improve microbial fuel cell (MFC) performance. Here we report a new functionalization process to improve interfacial electron transfer, biocompatibility and corrosion resistance of stainless steel (SS) electrodes used as anodes in MFCs. SS anodes prepared by surface modification with a t...

2 Laboratoire génie des procédés et environnement, équipe analyse chimiques et biocapteurs, faculté des sciences et techniques B.P. 146, Mohammedia, Université Hassan II, Abstract: The qualities of municipal and industrial wastewater vary over time and have a direct impact on the performance of wastewater treatment plants (WWTPs). The presence of a...

This work focused on the introduction of electrochemical moieties (electroactive molecules) onto the surface of magnetic nanoparticles (NPs) based on carbodiimide coupling chemistry and the influence of magnetic attraction at the working electrode surface on their electrochemical behaviour. The magnetic NPs were first functionalized with polyamidoa...

The efficient conjugation of ferrocenecarboxylic acid (Fc), a usual electroactive probe, onto COOH-modified magnetic nanoparticles (NPs) is reported. Amine crosslinkers were used to couple COOH groups from NPs and from Fc. The coupling efficiencies using different generations of polyamidoamine (PAMAM) dendrimer and ethylenediamine (EDA) were compar...

Carbon materials are considered building blocks for most of electrochemical sensors. Their biocompatibility allows their use as transducers for biosensors. Furthermore, they can be patterned, giving interest in all-inclusive bioelectrochemical detection devices. In particular, graphene, boron doped diamond, and pyrolyzed photoresist films are suita...

In order to optimize energy production in MFCs, a better understanding of anodic communities is essential. Our objective was to determine the taxonomic structure of the bacterial communities present at the surface of the anode during the formation and development of electro-active biofilms in MFCs inoculated with fresh primary clarifier overflow. Q...

In this paper, we describe the preparation and characterization of a thin silver film deposited on a non-conducting epoxy glass (EG) substrate, previously sensitized with tin chloride (SnCl2) by electroless plating technique. The X-ray diffraction (XRD) analysis of the deposits showed pure crystalline structure with grain size of about 20 nm. The e...

The invention relates to a process for treating a liquid effluent comprising an organic pollutant, said process producing electrical energy, characterized in that: the liquid effluent has a pH belonging to the range extending from 2 to 4 and contains aqueous hydrogen peroxide solution, at least one anode and one cathode are submerged in the liquid...

A study of 4H-SiC annealing temperature was performed using a rf-induction furnace in order to determined the optimal conditions to obtain suitable graphene layers. The graphene layer has been identified and confirmed by electrical measurements and physico-chemical analyses using Atomic Force Microscopy, X-Ray Photoelectron Spectroscopy and Infra-R...

Horizontal gene transfers are critical mechanisms of bacterial evolution and adaptation that are involved to a significant level in the degradation of toxic molecules such as xenobiotic pesticides. However, understanding how these mechanisms are regulated in situ and how they could be used by man to increase the degradation potential of soil microb...

A new polycyclic compound, 1,1′-{1,4-phenylene bis[3-(6-chloro-2-methyl-4-phenylquinolin-3-yl)-4,5-dihydro-1H-pyrazole-5,1-diyl]}dibutan-1-one (3) has been synthesized by cyclocondensation of (2E,2′E)-1,1′-bis(6-chloro-2-methyl-4-phenylquinolin-3-yl)-3,3′-(1,4-phenylene)diprop-2-en-1-one (2) and hydrazine hydrate in butanoic acid. The structure of...

Creating cell aggregates of controlled size and shape and patterning cells on substrates using a bottom-up approach constitutes important challenges for tissue-engineering applications and studies of cell-cell interactions. In this paper, we report nDEP (negative dielectrophoresis) driven assembly of cells as compact aggregates or onto defined area...

L'invention concerne un procédé de traitement d'un effluent liquide comprenant un polluant organique, ledit procédé assurant la production d'énergie électrique, caractérisé en ce que : - l'effluent liquide présente un pH appartenant à la gamme allant de 2 à 4 et contient de l'eau oxygénée, - au moins une anode et une cathode plongent dans l'effluen...

A culture independent approach was developed for recovering individual bacterial cells out of communities from complex environments including soils and sediments where autofluorescent contaminants hinder the use of fluorescence based techniques. For that purpose fifty nanometer sized streptavidin-coated superparamagnetic nanoparticles were used to...

We describe a new, simple process for fabricating transparent quadrupolar electrode arrays enabling large-scale particle assembly by means of dielectrophoresis. In a first step, interdigitated electrode arrays are made by chemical wet etching of indium tin oxide (ITO). Then, the transition from a bipolar to a quadrupolar electrode arrangement is ob...

The objective of this study was to evaluate the immobilization of lipase on an organic matrix by reticulation method, aiming at its application in the detection of organochlorine pesticide. The biologically inactive protein of bovine serum albumin (BSA) and glutaraldehyde were used for immobilizing lipase from a microbial source ( Candida rugosa CR...

In this paper, we demonstrate the possibility to trap and sort labeled cells under flow conditions using a microfluidic device with an integrated flat micro-patterned hard magnetic film. The proposed technique is illustrated using a cell suspension containing a mixture of Jurkat cells and HEK (Human Embryonic Kidney) 293 cells. Prior to sorting exp...

This study aims to examine the influence of a 0.5 T static magnetic field (SMF) on yeast and human embryonic kidney (HEK) 293 cell using electrorotation (ROT). Following 48 h exposition to the SMF, no difference was noted between ROT spectra of unexposed and exposed yeast cells, which extend previous reports on the absence of SMF effects on yeast....

This paper describes a new technique for focusing bacteria in a microfluidic channel and subsequently controlling their trajectory. Bacteria alignment is obtained using standing surface acoustic waves (SSAW) generated by two interdigitated transducer electrodes (IDTs) patterned on a piezoelectric wafer. The bacteria are focused in the standing wave...

This paper demonstrates the potential use of a new microfluidic device embedding thick electrodes for cell lysis and cell separation applications. The system consists of a microfluidic channel featuring conductive walls made of a polydimethylsiloxane (PDMS) matrix mixed with carbon nanoparticles. Cell lysis was performed electrically by applying sq...

Trapping of cells is essential to perform basic handling operations in cell-based microsystems, such as media exchange, concentration, cell isolation and cell sorting. Cell trapping by magnetophoresis typically requires cell labeling with magnetic nanoparticles. Here we report on endocytotic uptake of 100 nm magnetic nanoparticles by Human Embryoni...

High performance hard magnetic films (NdFeB, SmCo) have been patterned at the micron scale using thermo-magnetic patterning. Both out-of-plane and in-plane magnetized structures have been prepared. These micromagnet arrays have been used for the precise positioning and alignment of superparamagnetic nano- and microparticles. The specific spatial ar...

The low-cost prototyping of complex lab-on-chip systems requires the development of simple techniques for the reversible bonding of microfluidic channels onto different substrates. The authors report on the use of dimethyl methylphenylmethoxy siloxane (DMPMS) spin-coated layers for the bonding of polydimethylsiloxane (PDMS) microchannels onto a sub...

This paper reports on the integration of thick carbon-polydimethylsiloxane (C-PDMS) electrodes in microfluidic systems for electrokinetic operations. The C-PDMS material, obtained by mixing carbon nanopowder and PDMS, preserves PDMS processing properties such as O2 plasma activation and soft-lithography patternability in thick or 3D electrodes. Con...

This paper presents the most commonly used method to characterize individual biological cells on a dielectric point of view. It is a force based technique which lays on dielectrophoresis and/or electrorotation. First the principle of these phenomena are described and analyzed with an extension to magnetic forces at the micrometric scale level. Seco...

In this paper, we describe the use of Giant Unilamellar Vesicles (GUV) as cellular models to illustrate the possibility to trap magnetically-marked cells on a flat micro-patterned hard magnetic film by magnetophoresis. GUV can encapsulate nano-or micro-functional objects during the electroformation process and the amount of magnetic material incorp...

A simple method combining magnetic field and microparticles has been designed for assembling, locating and grafting permanent microfilaments onto a silanised glass surface. Filaments orthogonal to a glass support are implemented by assembling magnetic carboxylatex particles under magnetic field. They exhibit an aspect ratio up to 14. Carboxyl moiet...

The manipulation of magnetically labeled bio-objects of nano or micrometer sizes is now realizable by combining the magnetic forces with microfluidics. This paper reports a novel method for manipulating, trapping and sorting magnetic beads and magnetically labeled cells under continuous flow. This method uses a microfluidic device with integrated f...

This paper reports on the integration and evaluation of robust, thick Carbon-PDMS nanocomposite electrodes (C-PDMS), integrated in PDMS microfluidic systems to carry out electric field induced fusion of biological cells. Such material preserves PDMS processing properties and sustains a large range of electric field intensities and frequencies witho...

A method for the production of a biofilm at the surface of an electrode in a liquid medium containing bacteria and a substrate for growth of the bacteria, in which a system of electrodes constituted of two electrodes, which are connected to a direct electric current source, is used, these two electrodes are placed in the medium and a predetermined...

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The microbial fuel cell (MFC) technology could provide opportunities for electricity generation from the organic matter. Indeed a MFC can produce electric energy by converting a part of the chemical energy contained in biodegradable substrates through the metabolic activity of bacteria. This could be both economically and environmentally effective...

This paper focuses on the application of magnetophoresis to a new cell patterning method. The principle was demonstrated by using a CoPt micromagnet array, producing regularly spaced magnetic traps where cells were confined without any contact under the effect of negative magnetophoresis. To obtain this effect, yeast cells (Saccharomyces cerevisiae...

We demonstrate herein a newly developed photoelectrochemical immunosensor for the determination of anti-cholera toxin antibody by using a photosensitive biotinylated polypyrrole film. The latter was generated by electro-oxidation of a biotinylated tris(bipyridyl) ruthenium(II) complex bearing pyrrole groups. The photoexcitation of this modified ele...

This article explores the possibilities of using Sb doped SnO2 thin films as potential electrode substrates for biosensors. Two series of electrodes were modified with electrogenerated functionalised polymeric films. The polymers investigated were biotinylated poly(ruthenium pyrrole) and poly(pyrrole benzophenone). AFM studies show polymer morpholo...

This contribution reports, for the first time, the synthesis and electropolymerization of a pyrrole N-substituted by a nitrilotriacetic acid acting as a chelating center of Cu2+. A step-by-step approach for protein immobilization was developed via the successive coordination of Cu2+ and histidine-tagged proteins. The self-assembly of histidine-tagg...

The synthesis of a ruthenium (II) tris(bipyridyl) complex and its electropolymerization providing a biotinylated and photosensitive polymer were reported. The anchoring of the Cholera toxin onto this polypyrrolic film was carried out via affinity interactions based on avidin-biotin bridges between the polymer and the toxin. The photoelectrochemical...

A biotinylated photosensitive polymer was electrogenerated from on a ruthenium complex bearing biotin and pyrrole groups; the resulting polypyrrolic film allowed the bioaffine immobilisation of avidin and biotinylated cholera toxin and the photoelectrochemical detection of the corresponding antibody.

The bioaffine immobilization of several avidin layers on an electrode modified by a biotinylated polymer was accomplished by the first biotinylated redox bridge consisted of a tris(bipyridyl)iron(II) complex bearing six pre-oriented biotin groups.