Nancy A Monteiro-Riviere

Kansas State University and North Carolina State University · Director and Founder, Nanotechnology Innovation Center of Kansas State (NICKS)

Low tumor delivery efficiency is a critical barrier in cancer nanomedicine. This study reports an updated version of “Nano-Tumor Database”, which increases the number of time-dependent concentration data sets for different nanoparticles (NPs) in tumors from the previous version of 376 data sets with 1732 data points from 200 studies to the current...

The critical barrier for clinical translation of cancer nanomedicine stems from the inefficient delivery of nanoparticles (NPs) to target solid tumors. Rapid growth of computational power, new machine learning and artificial intelligence (AI) approaches provide new tools to address this challenge. In this study, we established an AI-assisted physio...

The functional activities of gold nanoparticles (AuNPs) on biological systems depend on their physical-chemical properties and their surface functionalizations. Within a biological environment and depending on their surface characteristics, NPs can adsorb biomolecules (mostly proteins) present in the microenvironment, thereby forming a dynamic biom...

Background Physiologically based pharmacokinetic (PBPK) modeling is an important tool in predicting target organ dosimetry and risk assessment of nanoparticles (NPs). The methodology of building a multi-route PBPK model for NPs has not been established, nor systematically evaluated. In this study, we hypothesized that the traditional route-to-route...

Background: Low delivery efficiency of nanoparticles (NPs) to the tumor is a critical barrier in the field of cancer nanomedicine. Strategies on how to improve NP tumor delivery efficiency remain to be determined. Methods: This study analyzed the roles of NP physicochemical properties, tumor models, and cancer types in NP tumor delivery efficien...

Immuno‐oncotherapy has shown great promise for the cure of late‐stage and metastatic cancer. Great efforts have tried to improve the overall response rate (ORR) and to reduce the immune‐related adverse events (irAEs). Antigen presentation, T cell activation and killing are interlocking and distinct steps to initiate effective anti‐tumor immune resp...

The cellular protein quality control (PQC) system ensures the intracellular misfolded/unfolded proteins to be detected and eliminated. ER-associated degradation (ERAD) and unfolded protein response (UPR) are the key mechanisms of PQC, which maintain protein homeostasis and ensure cell survival. Here, we show that after internalization by human epit...

Numerous studies have engineered nanoparticles with different physicochemical properties to enhance the delivery efficiency to solid tumors, yet the mean and median delivery efficiencies are only 1.48% and 0.70% of the injected dose (%ID), respectively, according to a study using a non-physiologically based modeling approach based on published data...

Aberrant splicing and protein interaction of Ras binding domain (RBD) is associated with melanoma drug resistance. Here cobalt or nickel doped zinc oxide (ZnO) physiometacomposite (PMC) materials bind to RNA and peptide shown by Ninhydrin staining, UV/Vis, FT-IR and CD spectroscopy. PMC deliver splice switching oligomer (SSO) into melanoma cells or...

Purpose: The aim of this study was to evaluate and compare the toxicity of six different types of titanium dioxide (TiO2) nanoparticles (NP) on human epidermal keratinocytes (HEK). Materials and methods: Six TiO2 NP (A (10nm), A*(32nm), B (27.5nm), C (200nm), C*(30-40nm) and D*(200-400nm)) were suspended in water or culture medium, and characterize...

A detailed understanding of the factors governing nanomaterial biodistribution is needed to rationally design safe nanomedicines. This research details the pharmacokinetics of gold nanoparticle (AuNP) biodistribution after arterial infusion of 40 or 80 nm AuNP (1 μg/ml) into the isolated perfused porcine skin flap (IPPSF). AuNP had surface coatings...

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This study aimed to conduct an integrated and probabilistic risk assessment of gold nanoparticles (AuNPs) based on recently published in vitro and in vivo toxicity studies coupled to a physiologically based pharmacokinetic (PBPK) model. Dose–response relationships were characterized based on cell viability assays in various human cell types. A prev...

The role of the skin as a potential route of exposure to nanomaterials is described in the present chapter. We survey the existing literature on various types of nanomaterials with respect to their penetration through the skin and toxicological responses. Most studies suggest minimal skin penetration and little to no systemic exposure. However, stu...

Nanoparticles (NPs) are easily contaminated by bacterial endotoxin (lipopolysaccharide [LPS]). The presence of LPS can be responsible for many immune/inflammatory effects attributed to NPs. In this study, we examined the effects of LPS adsorption on the NP surface on the formation of a biocorona in biological fluids and on the subsequent inflammati...

This study investigated the role of nanoparticle size and surface chemistry on biocorona composition and its effect on uptake, toxicity and cellular responses in human umbilical vein endothelial cells (HUVEC), employing 40 and 80 nm gold nanoparticles (AuNP) with branched polyethyleneimine (BPEI), lipoic acid (LA) and polyethylene glycol (PEG) coat...

Gold nanoparticles (AuNP) adsorb macromolecules to form a protein corona (PC) after systemic delivery, to which the kidney as the primary excretory organ is constantly exposed. The role of the PC on AuNP cell uptake and toxicity was investigated in vitro in human proximal tubule cells (HPTC) using 40 and 80 nm branched polyethylenimine (BPEI), lipo...

Protein corona formation over gold nanoparticles (AuNP) can modulate cellular responses by altering AuNP physicochemical properties. The liver plays an essential role in metabolism, detoxification and elimination of xenobiotics and drugs as well as circulating NP clearance. We investigated human hepatic uptake of 40 and 80nm AuNP with branched poly...

Aim: To assess inflammation, cellular uptake and endocytic mechanisms of gold nanoparticles (AuNP) in human epidermal keratinocytes with and without a protein corona. Materials & methods: Human epidermal keratinocytes were exposed to 40 and 80 nm AuNP with lipoic acid, polyethylene glycol (PEG) and branched polyethyleneimine (BPEI) coatings with...

Despite colloidal gold nanoparticles (AuNP) being proposed for a multitude of biomedical applications, there is a lack of understanding on how the protein corona (PC) formation over AuNP influences its interaction with blood components. Herein, 40 and 80 nm AuNP with branched polyethylenimine, lipoic acid, and polyethylene glycol surface coatings w...

Metallic nanoparticles ( NP ) have been used for biomedical applications especially for imaging. Compared to nonmetallic NP , metallic NP provide high contrast images because of their optical light scattering, magnetic resonance, X‐ray absorption, or other physicochemical properties. In this review, a series of in vitro imaging techniques for metal...

Quantitative analysis of the interactions between nanomaterials and environmental contaminants, such as pesticides, in natural water systems and food residuals is crucial for the application of nanomaterials-based tools for the detection of the presence of toxic substances, monitoring pollution levels, and environmental remediation. Previously the...

Threshold of Toxicological Concern (TTC) aids assessment of human health risks from exposure to low levels of chemicals when toxicity data are limited. The objective here was to explore the potential refinement of exposure for applying the oral TTC to chemicals found in cosmetic products, for which there are limited dermal absorption data. A decisi...

This review shows the skin is an important route of exposure to nanomaterials after occupational, environmental, and consumer product usage. The primary barrier to chemical and nanomaterial absorption is the rate-limiting stratum corneum layers and its lipid matrix. Nanoparticles of different size, shape, charge, surface properties, and vehicle as...

Aim: To develop a comprehensive computational framework to simulate tissue distribution of gold nanoparticles (AuNP) across several species. Materials & methods: This framework was built on physiologically based pharmacokinetic modeling, calibrated and evaluated with multiple independent datasets. Results: Rats and pigs seem to be more appropr...

To begin development of a mechanistically relevant humane alternative platform for safety assessment of dog food ingredients, comparative in vitro cytotoxicity of 20 ingredients was assessed in four canine cell types relevant for toxicity assessments. Previously, we described the toxicity of 13 compounds (clove leaf oil, eugenol, guanosine monophos...

Nanoparticles (NPs) are widely used in various fields of nanomedicine. A systematic understanding of NP pharmacokinetics is crucial in their design, applications, and risk assessment. In order to integrate available experimental information and to gain insights into NP pharmacokinetics, a membrane-limited physiologically based pharmacokinetic (PBPK...

We developed an in vitro method to assess pet food ingredients safety. Canine bone marrow-derived mesenchymal stem cells (BMSC) were differentiated into enterocyte-like cells (ELC) to assess toxicity in cells representing similar patterns of exposure in vivo. The toxicological profile of clove leave oil, eugenol, guanosine monophosphate (GMP), GMP...

In the past few years, there has been an unprecedented development of gold nanomaterials (AuNMs) for potential clinical applications. Owing to their advantageous physical, chemical, and biological properties, AuNMs have attracted great attention in the nanomedicine arena for applications in biological sensing, biomedical imaging, drug delivery, and...

This research aimed to develop in vitro methods to assess hazard of canine food ingredients. Canine hepatocytes were harvested and cell viability of clove-leaf oil (CLO), eugenol (EUG), lemongrass oil (LGO), guanosine monophosphate (GMP), inosine monophosphate (IMP), sorbose, ginger-root extract (GRE), cinnamon-bark oil (CBO), cinnamaldehyde (CINA)...

The role of nanoparticle (NP) interaction with biomolecules to form a biomolecular corona is the key to NP behavior and its consequences in the physiological environment. Since the adsorbed biocorona decides the fate of a nanomaterial in vivo, and thus its successful application in the biomedical arena, a comprehensive understanding of the dynamic...

Skin is an important route of exposure to chemicals after occupational, environmental, and consumer product usage. Knowledge of the rate and extent of skin absorption is required for risk assessments to be made. The primary barrier to chemical absorption is the stratum corneum. Transport across this barrier is assumed to occur primarily by diffusio...

Metallic nanoparticles ( NPs ) have been widely applied in the field of nanomedicine. A comprehensive understanding of their pharmacokinetics is crucial for proper risk assessment and safe biomedical applications. This review focuses on gold and silver (Ag) NPs , and briefly discusses iron oxide, titanium dioxide ( TiO 2 ), and zinc oxide NPs . Pha...

Quantitative characterization of nanoparticles interactions with their surrounding environment is vital for safe nanotechnological development and standardization. A recent quantitative measure, the Biological Surface Adsorption Index (BSAI), has demonstrated promising applications in nanomaterial surface characterization and biological/environment...

Aim: To assess the impact of biocorona kinetics on expected tissue distribution of nanoparticles (NPs) across species. Materials & methods: The potential fate of NPs in vivo is described through a simple and descriptive pharmacokinetic model using rate processes dependent upon basal metabolic rate coupled to dynamics of protein corona. Results:...

Molecular layer deposition (MLD) is a sequential and self-limiting process that may be used to create hybrid organic/inorganic thin films from organometallic precursors and organic alcohol precursors. In this study, films of a zirconium-containing hybrid organic/inorganic polymer known as zircone were grown on nanoporous alumina using MLD. Scanning...

This second edition documents the continuing development of essential tools for characterization of engineered nanomaterials (ENM) and in vitro and in vivo toxicity testing and the contribution of nanosafety research to nanomedicine. Topics include the impacts of nanotechnology on biomedicine, requirements for measurement and assessment, modeling,...

Silver nanoparticles (Ag-nps) are currently used as a natural biocide to prevent undesired bacterial growth in clothing, cosmetics and medical products. The objective of the study was to impart antibacterial properties through the incorporation of Ag-nps at increasing concentrations to electrospun degradable 50:50 poly(L-lactide-co-epsilon-caprolac...

The field of nanomaterial pharmacokinetics is in its infancy, with major advances largely restricted by a lack of biologically relevant metrics, fundamental differences between particles and small molecules of organic chemicals and drugs relative to biological processes involved in disposition, a scarcity of sufficiently rich and characterized in v...

Over the past decade, the field of nanoscience has grown tremendously because nanomaterials have widespread applications in material science, engineering, and medicine. Although this work has received a great deal of attention, many challenges must be overcome before nanotechnology can be routinely applied in nanomedicines or in consumer products....

Confocal laser scanning microscopy (CLSM) is a well-used microscopic tool that provides valuable morphological and functional information within cells and tissues. The application of CLSM to skin and the topical penetration of nanoparticles (NP) will be addressed. First, we describe the advantages of confocal microscopy compared to other techniques...

Nanoparticles (NP) absorbed in the body will come in contact with blood proteins and form NP/protein complexes termed protein coronas, which may modulate NP cellular uptake. This study quantitated human epidermal keratinocyte (HEK) uptake of silver (Ag) NP complexed to different human serum proteins. Prior to HEK dosing, AgNP (20nm and 110nm citrat...

Nanomaterials play a significant role in biomedical research and applications because of their unique biological, mechanical, and electrical properties. In recent years, they have been utilized to improve the functionality and reliability of a wide range of implantable medical devices ranging from well‐established orthopedic residual hardware devic...

Microneedles are small-scale devices that may be used for drug delivery and biosensing. In this study, the forces required for mechanical failure, the modes of mechanical failure, as well as the mechanisms for microneedle penetration into porcine skin were examined. Microneedles produced from the acrylate-based polymer e-Shell 200 using an indirect...

The role of the skin as a potential route of exposure to nanomaterials is described in the present chapter. Most studies suggest minimal skin penetration and little to no systemic exposure. However, they also show that nanoparticle (NP) size, shape, charge, surface properties, and vehicle as well as animal species are very important determinants as...

The costs associated with the treatment of medical device and surgical site infections are a major cause of concern in the global healthcare system. To prevent transmission of such infections, a prophylactic surface system that provides protracted release of antibacterial silver ions using low intensity direct electric current (LIDC; 28 μA system c...

Nanomaterials increasingly are playing a role in society for uses ranging from biomedicine to microelectronics; however, pharmacokinetic studies, which will be necessary for human health risk assessments, are limited. Currently the most widely used nanoparticle in consumer products is silver (Ag). The objective of the present study was to quantify...

Tissue barbs are small-scale structures that may be used for sutureless joining of tissues. In this study, several types of tissue barbs were fabricated using two-photon polymerization/micromolding, including two-pronged tissue barbs, eight-pronged tissue barbs, 10-pronged tissue barbs, and 16-pronged tissue barbs. Tissue barb penetration in porcin...

A number of commercially available metal/metal oxide nanoparticles (NPs) such as superparamagnetic iron oxide (SPION) are utilized by the medical field for a wide variety of applications. These NPs may able to induce dermal toxicity via their physical nature and reactive surface properties. We hypothesize that SPION may be toxic to skin via the abi...

To evaluate the toxicity and cellular uptake of both undifferentiated and differentiated human adipose-derived stem cells (hASCs) exposed to silver nanoparticles (Ag-NPs), and to assess their effect on hASC differentiation. hASC were exposed to 10- or 20-nm Ag-NPs at concentrations of 0.1, 1.0, 10.0, 50.0 and 100.0 µg/ml either before or after diff...

Hydroxylated fullerenes (C₆₀OH(x)) or fullerols are water-soluble carbon nanoparticles that have been explored for potential therapeutic applications. This study assesses acute in vivo tolerance in 8-wk-old female Sprague-Dawley rats to intravenous (iv) administration of 10 mg/kg of well-characterized C₆₀(OH)₃₀. Complete histopathology and clinical...

Baynes, R., Riviere, J., Franz, T., Monteiro-Riviere, N., Lehman, P., Peyrou, M., Toutain, P.-L. Challenges obtaining a biowaiver for topical veterinary dosage forms. J. vet. Pharmacol. Therap.35 (Suppl. 1), 103–114. Obtaining a biowaiver for topical drugs used in veterinary species faces many of the same challenges associated with human topicals....

Ultrananocrystalline diamond (UNCD) exhibits excellent biological and mechanical properties, which make it an appropriate choice for promoting epidermal cell migration on the surfaces of percutaneous implants. We deposited a ~150 nm thick UNCD film on a microporous silicon nitride membrane using microwave plasma chemical vapor deposition. Scanning...