Nanang Fakhrudin

Nanang Fakhrudin
Universitas Gadjah Mada | UGM · Department of Pharmacy

Doctor of Pharmacy

About

63
Publications
14,623
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861
Citations
Citations since 2016
22 Research Items
605 Citations
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2016201720182019202020212022020406080
2016201720182019202020212022020406080
2016201720182019202020212022020406080
Additional affiliations
February 2003 - November 2015
Universitas Gadjah Mada
Position
  • Researcher
January 2003 - November 2015
Universitas Gadjah Mada
Position
  • Researcher

Publications

Publications (63)
Article
Coffee contains caffeine and chlorogenic acid (CGA) as the main constituent benefiting human health. Extract of green coffee beans (GCB) has some limitations in terms of its unpleasant flavour, aroma, and phytoconstituents bioactivity. This study aimed to encapsulate the crude ethyl acetate (EtOAc) extracts of Gayo Robusta GCB (Coffea canephora) to...
Article
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Platelet plays a crucial role in cardiovascular diseases (CVDs) development. Abnormalities in platelet aggregation provokes thrombo-embolism, eventually leading to death. In Indonesia, breadfruit (Artocarpus altilis) leaf is traditionally used to treat CVDs. This study aimed to evaluate the antiplatelet activity of A. altilis leaf extract (AAE) and...
Article
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p>Biji dan daun kacang hijau (KH) mengandung senyawa flavonoid sebagai antioksidan yang bermanfaat bagi kesehatan. KH dapat diekstrasi dengan air dan etanol menghasilkan ekstrak kaya flavonoid untuk bahan baku produk suplemen kesehatan dan obat tradisional. Penelitian bertujuan membandingkan potensi antioksidan dari biji dan daun KH, menganalisis k...
Article
Indonesia merupakan negara tropis dengan intensitas sinar matahari yang tinggi sehingga hal ini dapat memberikan dampak negatif terhadap kulit manusia. Untuk mencegah kerusakan akibat dari cahaya matahari diperlukan suatu bahan yang bersifat fotoprotektor dengan mekanisme kerja menyerap atau menghalangi cahaya matahari masuk ke kulit. Flavonoid eks...
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Piper acre Blume, known as Black Betel (local name), is a plant that is widely used by the people of East Kalimantan, especially in Samarinda, for the treatment of illness. Leaves (3-4 months old) are collected from Samarinda, extracted, fractionated, and monitored by DPPH antiradical activity. The isolation of the Piper acre Blume is performed on...
Article
Full-text available
Objective: This study aimed to evaluate the cytotoxic activity of the extracts and fractions of Eriocaulon cinereum against HeLa and Vero cell lines,which represent cervical cancer and normal cells, respectively. In addition, a phytochemical screening was carried out to determine the chemicalconstituents in the extracts and the active fractions.Mat...
Article
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Senyawa fenolik mempunyai potensi sebagai fotoprotektor, sehingga mampu melindungi kulit dari kerusakan oksidatif terutama menyerap Ultra Violet dari pancaran sinar matahari. Ekstrak etanolik buah masak mahkota dewa [Phaleria macrocarpa (Scheff) Boerl.] mengandung senyawa fenolik dan memiliki peranan sebagai fotoprotektor. Penelitian ini bertujuan...
Article
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Brown macroalgae (BM) which contain fucoxanthin exhibited high antioxidant activity. This study was performed to examine antioxidant, anti-collagenase, anti-elastase, anti-tyrosinase activities, and effect on cell viability of Human Dermal Fibroblast adult (HDFa) of BM, Turbinaria decurrens Bory. T. decurrens dried powder were macerated by ethanol...
Article
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Background: The effects of tylophorine, a natural alkaloid found in Tylophora indica, administered as a single compound or in combination with doxorubicin on cell cycling and apoptosis were assessed in T47D breast cancer cells, selected as a model system for breast cancer. Methods: Cell cycle distribution and apoptosis were examined by flow cytomet...
Conference Paper
Full-text available
Plants of the genus Cinnamomum are known to have anti-thrombotic activity via the inhibition of platelet activation and improvement to blood circulation. Previous studies have indicated that Cinnamomum sintoc (CS) has a potent anti-platelet aggregation activity, which is induced by adenosine diphosphate (ADP). In this study, we report the anti-plat...
Article
Full-text available
Inflammation is involved in the progression of many disorders, such as tumors, arthritis, gastritis, and atherosclerosis. Thus, the development of new agents targeting inflammation is still challenging. Medicinal plants have been used traditionally to treat various diseases including inflammation. A previous study has indicated that dichloromethane...
Conference Paper
Red dragon fruit (Hylocereus polyrhizus) and carrot (Daucus carota L.) have been known for their phenolic and flavonoid compounds with antioxidants properties. Antioxidant activities including β-carotene bleaching (BCB) and Cupric ion Reducing Antioxidant Capacity (CUPRAC) and total phenolic content (TPC), total flavonoid content (TFC), and cytopro...
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Free radicals are ions, molecules or atoms with unpaired electrons that are highly unstable and reactive against other molecules. Free radicals contributed in the development of various human disorders. In this study, we investigated the possible new sources of natural radical scavengers which could provide antioxidant mechanisms to protect against...
Article
Full-text available
Hyperlipidemic is a lipid metabolism disorder characterized by high levels of blood cholesterol, triglycerides and LDL and low HDL levels. Hyperlipidemic is one of the most important risk factor of atherosclerosis leading to cardiovascular diseases. Breadfruit (Artocarpus altilis (Parkinson) Fosberg.) is an Indonesian native plant traditionally use...
Article
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In addition to the body's response to injury, inflammation is a physiological process underlying the progression various diseases. Investigation for the discovery and development of antiinflammatory agents from natural sources is a promising strategy for curing inflammatory diseases. Breadfruit or Artocarpus altilis, is an Indonesian native plant t...
Article
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In the search for peroxisome proliferator-activated receptor gamma (PPARγ) active constituents from the roots and rhizomes of Notopterygium incisum, 11 new polyacetylene derivatives (1-11) were isolated. Their structures were elucidated by NMR and HRESIMS as new polyyne hybrid molecules of falcarindiol with sesquiterpenoid or phenylpropanoid moieti...
Data
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Poster presented at 13th International Congress of the Society for Ethnopharmacology, Graz, Austria, September 2 – 6, 2012. This poster is published under a CC-BY license: http://dx.doi.org/10.6084/m9.figshare.1119208
Data
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Poster presented at EMBO Conference on Cellular Signaling & Molecular Medicine 25‐29 May 2012, Cavtat‐Dubrovnik, Croatia. This poster is published under a CC-BY license: http://dx.doi.org/10.6084/m9.figshare.1101323
Article
Plants used in traditional medicine represent an important source of new lead compounds. However, cell-based in vitro screening assays with plant material are hampered by the complex nature of plant extracts as mixtures of active and inactive components. Bulk constituents, such as chlorophyll and polyphenols were previously shown to interfere with...
Data
Cytotoxicity determination upon prolonged exposure and in the presence of higher concentrations of plumericin. HEK293/NF-κB-luc cells were treated as indicated in Figure 1A and Figure 1B with solvent vehicle (0.1% DMSO), different concentrations of plumericin, or digitonin (200 μg·mL-1) as positive control. LDH enzyme activity was determined 24 h a...
Data
Comparison of the effects of plumericin, parthenolide, and the H. sucuuba extract on NF-κB activity in the presence and absence of TNF-α stimulation. HEK293/NF-κBluc cells were pretreated for 30 min with H. sucuuba extract (60 μg·mL-1), plumericin (5 μμ), parthenolide (5 μμ), or solvent vehicle (DMSO 0.1%), and stimulated with 2 ng·mL-1 TNF-α or tr...
Data
Plumericin and the other investigated compounds do not affect the basal expression of the endothelial adhesion molecules VCAM-1, ICAM-1, and E-selectin in the absence of TNF-α stimulation. HUVECtert cells were treated with 10 μμ plumericin, parthenolide, plumieridin, allamandicin, plumeridoid C or 10 ng·mL-1 TNF-α for 14 h (VCAM-1, ICAM-1) or 5 h (...
Data
Effect of reference inhibitors on the investigated phosphorylation sites of JNK, p38, and ERK1/2. HUVECtert cells were pre-incubated for 30 min with SP600125 (50 μμ), forskolin (20 μμ), U0126 (10 μμ) or solvent vehicle (DMSO, 0.1% or 0.2%, respectively), prior to stimulation with TNF-α (10 ng·mL-1) for 10 min.Western blot analysis was performed for...
Data
Among all isolated compounds only plumericin is a potent inhibitor of TNF-α-induced NF-κB activation. HEK293/NF-κB-luc cells were pretreated for 30 min with the indicated compounds (10 μMeach) or solvent vehicle (DMSO 0.1%), and stimulated with 2 ng·mL-1 TNF-α for 4 h. The data represent mean ± SEM (n = 3; *P < 0.05, **P < 0.01, n.s. not significan...
Data
Plumericin but not the other structurally related compounds isolated from H. sucuuba inhibits TNF-α-induced cell surface expression of the endothelial adhesion molecules VCAM-1, ICAM-1, and E-selectin. HUVECtert cells were pretreated with 10 μμ plumericin, parthenolide, plumieridin, allamandicin, plumeridoid C or solvent vehicle (DMSO 0.1%) as cont...
Article
Full-text available
The transcription factor nuclear factor kappa B (NF-κB) orchestrates many pro-inflammatory signals and its inhibition is considered a promising strategy to combat inflammation. Here we report the characterization of the natural product plumericin as a highly potent inhibitor of the NF-κB pathway with a novel chemical scaffold, which was isolated vi...
Article
The transcription factor nuclear factor kappa B (NF-κB) plays a crucial role in the regulation of the inflammatory response and contributes to the development of various diseases (1). Thus, its inhibition is considered a promising approach to combat inflammation (2). In this study, we applied a bioactivity-guided approach to identify NF-κB inhibito...
Article
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Peroxisome proliferator-activated receptor gamma (PPARγ) agonists are clinically used to counteract hyperglycemia. However, so far experienced unwanted side effects, such as weight gain, promote the search for new PPARγ activators. We used a combination of in silico, in vitro, cell-based and in vivo models to identify and validate natural products...
Article
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Ethnopharmacological relevance: In Austria, like in most Western countries, knowledge about traditional medicinal plants is becoming scarce. Searching the literature concerning Austria's ethnomedicine reveals its scant scientific exploration. Aiming to substantiate the potential of medicinal plants traditionally used in Austria, 63 plant species o...
Article
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Beneficial effects of red wine polyphenols on cardiovascular health are well known. The aim of our research was an interaction study of four red wine polyphenols – resveratrol (R), quercetin (Q), kaempferol (KF) and isorhamnetin (IR) of their ability to activate endothelial NO synthase (eNOS) promoter when used alone and in equimolar mixtures. To d...
Article
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Peroxisome proliferator-activated receptor gamma (PPARγ) is a key regulator of glucose and lipid metabolism and therefore an important pharmacological target to combat metabolic diseases. Since the currently used full PPARγ agonists display serious side effects, identification of novel ligands, particularly partial agonists, is highly relevant. Sea...
Article
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Melampyrum pratense L. (Koch) is used in traditional Austrian medicine for the treatment of different inflammation-related conditions. In this work, we show that the extracts of M. pratense stimulated peroxisome proliferator-activated receptors- (PPARs-)α and -γ that are well recognized for their anti-inflammatory activities. Furthermore, the extra...
Article
Himatanthus sucuuba (Spruce) Woodson (Apocynaceae) has been traditionally used in South America to treat inflammatory diseases. Fractions from H. sucuuba have been shown to possess anti-inflammatory activity in vivo.¹ However, the active principles and involved targets remained unknown. Bioactivity-guided fractionation of bark material led to the i...
Article
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Chinese herbal medicinal (CHM) extracts from fourteen plants were investigated in cell-based in vitro assays for their effect on nuclear factor κB (NF-κB), a key regulator of inflammation, as well as on peroxisome proliferator-activated receptors (PPARs) being key regulators of genes involved in lipid and glucose metabolism. 43% of the investigated...
Data
Workflow of bioassayguided fractionation and isolation.
Article
In the course of our search for PPARγ active anti-inflammatory natural products we have investigated the dichloromethane extract of the dried rhizome and root of Notopterygium incisum Ting ex H. T. Chang (Umbelliferae). PPARγ is one of the three Peroxisome Proliferator Activated Receptor (PPAR) subtypes and is involved in the regulation of glucose...
Article
In our search for natural products activating PPAR-gamma we have isolated three polyacetylene ferulic acid conjugates (1-3) from the underground parts of Notopterygium incisum Ting ex H. T. Chang (Qiang Huo), in addition to previously isolated polyene-yne derived compounds.1,2 Their structures were elucidated by NMR and MS. Compound 1 is formed by...
Article
In recent years, increasing attention has been drawn towards the role of lipids in cell signalling pathways and regulation.(1) Plants are a rich source of bioactive lipids. We, therefore, focused on (complex) lipids from plants used as traditional Chinese medicinal (TCM) drugs and their influence on cellular processes. Four new cerebrosides, which...
Article
The roots of Krameria lappacea are used traditionally against oropharyngeal inflammation. So far, the astringent and antimicrobial properties of its proanthocyanidin constituents are considered to account for the anti-inflammatory effect. The aim of the present study was to characterize pharmacologically a lipophilic extract of K. lappacea roots an...
Article
Inhibition of vascular smooth muscle cell (VSMC) proliferation is of substantial interest in combating cardiovascular disease. A dichloromethane extract from the rhizomes of Peucedanum ostruthium, a traditionally used Austrian medicinal plant with anti-inflammatory properties, was examined for a putative antiproliferative activity in rat aortic VSM...
Article
Full-text available
Various inflammatory stimuli that activate the nuclear factor kappa B (NF-κB) signaling pathway converge on a serine/threonine kinase that displays a key role in the activation of NF-κB: the I kappa B kinase β (IKK-β). Therefore, IKK-β is considered an interesting target for combating inflammation and cancer. In our study, we developed a ligand-bas...
Article
Full-text available
Peroxisome proliferator-activated receptor gamma (PPAR gamma) agonists are used for the treatment of type 2 diabetes and metabolic syndrome. However, the currently used PPAR gamma agonists display serious side effects, which has led to a great interest in the discovery of novel ligands with favorable properties. The aim of our study was to identify...
Article
to investigate the cytotoxic and apoptotic effects of saponins from Plumeria acuminata Ait on oral squamous carcinoma cells (OSCC). OSCC cells seeded at 2 × 104 cells/well in a 96-well plate were treated with saponins from P. acuminata Ait and cisplatin in various concentrations for 24 h. Trypan blue dye exclusion assay and ethidium bromide/acridin...
Article
Various studies indicate that several ubiquitous plant compounds, like chlorophyll and tannins, possibly interfere with biological in vitro assays. Chlorophyll might interact with fatty acids, whereas tannins can form tight complexes with metal ions, proteins and polysaccharides [1]. The aim of this study was, to examine whether the chlorophyll and...

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