Naim Saglam

Firat University Fisheries Faculty · Department of Aquaculture and Fish Diseases

Objective: Medicinal leeches (Hirudo spp.) have been used for therapeutic purposes in humans since ancient times. Because of their growth conditions, leeches carry certain bacteria and endosymbionts (e.g., Aeromonas spp). In both leech farms and hirudotherapy clinics, there are no reliable antiseptics that can be used with leeches. This study aimed...

Historical climate data indicate that the Earth has passed through multiple geological periods with much warmer-than-present climates, including epochs of the Miocene (23–5.3 mya BP) with temperatures 3–4°C above present, and more recent interglacial stages of the Quaternary, for example, Marine Isotope Stage 11c (approx. 425–395 ka BP) and Middle...

The glossiphoniid leech, Helobdella austinensis, is an experimentally tractable member of the superphylum, Lophotrochozoa. Its large embryonic cells, stereotyped asymmetric cell divisions and ex vivo development capabilities makes it a favorable model for studying the molecular and cellular events of a representative spiralian. In this study, we fo...

Since its inception ~6000 years ago, aquaculture has evolved to enable its survival and growth to become a major contributor of protein of high nutritional value for human consumption, thereby improving food security and reducing poverty. Best practices have been established and updated to reflect developments as they occurred. Currently, best prac...

The aim of this study was to investigate effects of sulfamerazine on oxidative stress of rainbow trout (Oncorhynchus mykiss). The fish were divided into four groups. The first group was fed with diets containing no sulfamerazine; the second group was supplemented with sulfamerazine at 100 mg kg-1 for 21 days; the third group was treated with sulfam...

Parasites in which freshwater snails are intermediate hosts pose a serious threat to human health worldwide. We show here that freshwater snails can potentially be controlled by leech predation; in principle, this approach could significantly reduce snail-borne parasitic diseases (SBPDs). Specifically, glossiphoniid leeches, Helobdella austinensis...

The article describes the results of the study of leeches in the Fergana Valley, located in the eastern part of Uzbekistan. For the first time, the blood-feeding leech Limnatis paluda (Tennent, 1859) was recorded from the study area. It was found that small populations of this species are distributed in ten different biotopes of the Fergana Valley....

The leech samples were collected and identified in some wetlands of Türkiye between January 2009 and September 2016. During the study, leech samples were taken from nine wetlands. In the study, seven species belonging to Erpobdellidae (Erpobdella octoculata, Erpobdella testacea), Glossiphonidae (Helobdella stagnalis, Placobdella costata), Hirudinid...

Diplostomid trematodes (Digenea) include a wide and diverse group of common digeneans. Diplostomid larval stages are significant pathogens that may exert grave effects on both natural fish and aquaculture populations. Diplostomum species, which use fish as a second intermediate host, is one of the most common trematode species, which affects the fi...

Leeches are important and reliable indicators of water quality and biodiversity in the ecosystem, so the presence of specific leech species is often closely related to basic water conditions and the presence of certain animals. This study was carried out in 2017 and 2018 in order to determine the Hirudinea fauna of some wetlands in Bingöl province....

Bu çalışma, Ocak 2012-Aralık 2014 tarihleri arasında Güney Doğu Anadolu Bölgesinde yer alan yedi ilde (Adıyaman, Batman, Diyarbakır, Gaziantep, Kilis, Mardin ve Şanlıurfa) bulunan akarsu, baraj, göl, gölet ve sazlık alan olmak üzere toplam 169 tatlısu habitatında sülük faunasının belirlenmesi amacıyla yürütüldü. Sülük örnekleri habitatın özelliğine...

Bu çalışma, Yaban Hayatı'nda yakalanan üç farklı balık türündeki (Barbus lacerta, Capoeta trutta ve Capoeta umbla) parazitlerin (Myxobolus cyprini ve Lamproglena pulchella) enfeksiyon ve patolojilerini belirlemek için yapıldı. Bu çalışma için yakalanan balıklar, etik kurallara uygun olarak laboratuvara nakledildi ve parazit açısından araştırıldı. S...

ABSTRACT Although medicinal leeches are used for many years in Azerbaijan for therapeutic purposes, relatively little is still known about their distribution, economic Importance, and general status. The medicinal leech species of Azerbaijan was named Hirudo medicinalis or H. medicinalisorientalis and is still exported under the same names in CITES...

The bloodfeeding leech genus Placobdella is dominated by North American diversity, with only a single nominal species known from Central America and one from the Palearctic region. This is likely due to considerable underestimation of Palearctic biodiversity, but investigations into potential hidden diversity are lacking. To shed light on this, the...

This study is determined the molecular characterization of R. venosa based on the mitochondrial cytochrome c oxidase subunit I (COX1) sequence data in the Middle Black Sea of Turkey.

The present study aimed to investigate growth, survival and reproduction of the Turkish medicinal leech, Hirudo sulukii, endemic to Southeastern Anatolia and whose biology is poorly understood. In comparison with the Southern medicinal leech, Hirudo verbana, which has a wide distribution throughout Eurasia, H. sulukii grew faster until the fourth m...

Leeches have been used to complement medical therapies for many years, however there is little data on the microorganisms they may harbor as part of their flora. The study aims were to (1) identify the presence of bacteria and (2) the presence of Hepatitis B and C viruses (HBV and HCV) in medicinal leeches using traditional bacteriological assays,...

This study was carried out between May 2017 and December 2019 to determine leech species in some wetlands in the Eastern region of Azerbaijan. In the research, the leech samples were collected from 21 locations from six wetlands in the eastern part of Azerbaijan. As a result of field and laboratory studies, five species of leeches were determined (...

Medical leeches have been widely used in medical applications and treatments for millennia. Studies on the salivary glands of blood-sucking leeches have focused on their bioactive secretions and mechanisms of action, with little attention to ultrastructure. In this study, we examined dissected embryonic and adult Hirudo verbana salivary glands by s...

Trichlorfon (TCF) is an antiparasitic agent used in aquaculture to treat external parasites and it belongs to the chemical class of organophosphates. This study was conducted to investigate the ameliorating effects of lycopene on TCF-induced toxicity in carp by evaluating haematological parameters and oxidant/antioxidant status. Fish were randomly...

Objective: The aim of this study was to investigate the internal and external morphological structures of two species of medicinal leeches (Hirudo sulukii and Hirudo verbana). Methods: Leech specimens were collected from various regions of Turkey between the years of 1995-2016. The leeches were first examined alive and later were stunned with 10% e...

The leeches are one of the neglected organisms in the scientific studies. There are approximately 800 leech species, however a small number of them are classified as “medicinal leech”. They are used in veterinary, cosmetic, pharmacology and especially medicine areas. Because of both over collection and habitat destruction, the medicinal leech popul...

In a recently published article (Saglam et al. 2018) the name of a new species of Hirudinea (Annelida, Clitellata) was simultaneously published in three different ways, as Helobdella serendipitious (Saglam et al. 2018: 61, 70, 71, 73), Helobdella serendipitous (ibid.: 63, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74), and as Helobdella serendipidous (ibi...

Objective: The aim of this study was to investigate the internal and external morphological structures of two species of medicinal leeches (Hirudo sulukii and Hirudo verbana). Methods: Leech specimens were collected from various regions of Turkey between the years of 1995-2016. The leeches were first examined alive and later were stunned with 10% e...

In this study, effects of propolis on immunological parameters of rainbow trout were investigated. For this purpose, propolis was intraperitoneally injected to fish 4 times at doses of 2.5, 5 and 10 mg / kg fish weight. Blood samples on 3, 9, 15 and 21 days were collected from fish of the control and experimental groups and analysed to determine ox...

The glossiphoniid freshwater leech, Helobdella stagnalis, was described by Linnaeus 1758 based on common European specimens. The presence of a brown, chitinous scute on the dorsal-anterior surface, as observed on leeches elsewhere in the world, has generally led to the classification of all scute-bearing members of the genus as H. stagnalis. Here w...

Myzobdella lugubris is a commensal leech on crustaceans and a parasite to fishes, surviving predominantly in brackish waters throughout North America. Specimens in this study were collected within the tidal zone of the Delaware River basin (New Jersey and Pennsylvania). To compare regional M. lugubris specimens, defined characters were scored after...

Leeches are indispensable organisms of the aquatic ecosystem. The worldwide are identified over than 650 species of leeches. These organisms live in freshwater, estuarine, marine, and semi-terrestrial ecosystems. Leeches occur in slow flowing parts of streams and rivers, in artificial and natural lakes, in marine, in habitats such as marsh and lago...

Species of medicinal leeches (Hirudo medicinalis, H. verbana, and H. sulukii) secrete hard-shelled cocoons. When initially deposited, a cocoon is surrounded by a foam. Over a short time, the foam is transformed into a three-dimensional structure. We show here that this peripheral structure likely forms by the solidification and dehydration of a mod...

Segmented worms (Annelida) are among the most successful animal inhabitants of extreme environments worldwide. An unusual group of enchytraeid oligochaetes of genus Mesenchytraeus are abundant in the Pacific northwestern region of North America and occupy geographically proximal ecozones ranging from low elevation rainforests and waterways to high...

Çevresel toksinler, aşırı alınmış oksijen, çözücüler, petrokimya ürünleri gibi maddeler ile enfeksiyonlar ve bu enfeksiyonların tedavisi sırasında kullanılan kemoterapötikler vücutta serbest oksijen radikalleri oluşumuna neden olurlar. Seruloplazmin hem bakır hem de demir metabolizmasında görevli antioksidandır. Seruloplazmin süperoksit dismutaza b...

Background Species of Hirudo are used extensively for medicinal purposes, but are currently listed as endangered due to population declines from economic utilization and environmental pollution. In total, five species of Hirudo are currently described throughout Eurasia, with Turkey being one of the major exporters of medicinal leech, primarily H....

Bu calisma Ege Denizinin Canakkale Bolgesinden aylik olarak avlanan, toplamda 243 adet kolyoz ( Scomber japonicus ), 377 adet istavrit ( Trachurus trachurus ) ve 451 adet sardalya ( Sardinella aurita ) olmak uzere toplam 1071 adet balik uzerinde yapildi. Baliklarin parazitolojik muayenesinde, kolyozlarin 108, istavritlerin 140 ve sardalyalarin da 1...

Trichlorfon is among the most commonly used products to treat fish parasites in aquaculture. We investigated the effectiveness of propolis in alleviating the toxicity of trichlorfon on haematological and oxidant/antioxidant parameters in carp Cyprinus carpio. Fish were exposed to sublethal concentrations (11 and 22 mg l-1) of trichlorfon, and propo...

Investigation of endohelmintes in snail (Helix lucorum, Linneaus, 1758) living in Keban Region of Elaziğ. In this study, total 20 snails (Helix lucorum) obtained from Keban Region of Elazığ were examined interm of endohelmintes. At the end of parasitological examination of snails, Paragonimus sp. (Trematoda; Digenea) and Protostrogylus sp. (Nematod...

Farmed rainbow trout (Oncorhynchus mykiss) were examined for anisakid nematodes at fish farms in Elazıg city, Turkey. A total of 439 fish (246 from fish farm ponds and 193 from net-cages) were monthly investigated in the period from February 2000 to May 2003. Only the endoparasite, hysterothylacium aduncum (Nematoda: Anisakidae) was recorded in the...

Medicinal leeches are known to inject more than 100 bioactive substances in the tissue whilst sucking blood. They are being successfully used for the treatment of diseases and symptoms, especially in plastic surgery. We report the case of a 7-year old boy, who had lost the first phalanx of the middle finger of the right hand after an accident. The...

Clitellate annelids (i.e., oligochaetes including leeches) secrete cocoons as part of their normal reproductive cycle. Typically, the cocoon sheath is passed over the head of the leech and sealed at both ends by opercula (i.e., glue-like material secreted by the clitellum). Both the fibrous cocoon wall (CW) and opercula are chemically-related bioma...

Clitellate annelids (i.e., oligochaetes including leeches) secrete cocoons as part of their nor-mal reproductive cycle. Typically, the cocoon sheath is passed over the head of the leech and sealed at both ends by opercula (i.e., glue-like material secreted by the clitellum). Both the fibrous cocoon wall (CW) and opercula are chemically-related biom...

Freshwater snails play a significant role as intermediate host in life cycles of trematodes. In addition to providing these parasites with a site for reproduction, freshwater snails are also carriers to reach them to their next hosts. It is obvious that infected snail carrying cercaria or metacercaria play an important role in the distribution of t...

This study has been carried out to investigate the presence of Yersinia ruckeri, the causative agent of Enteric Red Mouth (ERM) disease, in rainbow trout fish breeding farms located in Elazig and Malatya provinces in Eastern Turkey. For this purpose, bacteriological culture and a specific polymerase chain reaction (PCR) have been compare with exami...

In this study, effects of ivermectin on selected immunological parameters, oxidative stress, and antioxidant status in the blood, liver, kidney, heart, and spleen of the rainbow trout (Oncorhynchus mykiss) were examined. Ivermectin was administered intraperitoneally at single doses of 0.01 and 0.02 mg ivermectin kg -1 fish. Blood and tissue samples...

Leeches have been used in the treatment of certain diseases since ancient times. There are a few species of medicinal leeches. However, H. medicinalis and H. verbana are known in Turkey. Currently, Turkey is one of the world's most important leech-exporting countries. Secretion of the salivary glands of medical leeches contains more than 100 bioact...

zet: Bu çalışmanın hazırlanması amacıyla en büyük sülük ihracatı potansiyeline sahip olan Samsun ilindeki sulak alanlarda tıbbi sülük toplayanlarla temasa geçildi. Bölgede yer alan bazı sulak alanlara gidilerek ihraç ürünü olan sülüklerden örnekler alındı ve tür teşhisleri yapıldı. Ticari amaçla sülük toplayıcılığı yapanların toplama çalışmaları ye...

In this study, the effects of lycopene on some haematological and immunological parameters of common carp (Cyprinus carpio) were examined. The fish were divided into four groups. The first group was regarded as the control group. Corn oil was applied to fish in group 2. 5 and 10 mg lycopene were orally administered to fish in group 3 and group 4, r...

In this present study, the plasma ceruloplasmin (Cp) level was examined in rainbow trout (Oncorhynchus mykiss, W.) feed on a diet with three different doses (100, 200 and 400 mg/kg) of sulfamerazine. Four groups of rainbow trout (Oncorhynchus mykiss) were fed experimental diets containing either no sulfemerazine (control) or supplemented with sulfa...

An outbreak has appeared affecting rainbow trout weighting 0.5-1 g from different fish man- agement units in Kahramanmaras region in 2008 year. The fish were examined systematically in terms of fish diseases. Fish samples were brought Firat University Fisheries Faculty. For this goal, isolation was made onto appropriate agar plates under sterile co...

In this study, effects of Yersinia ruckeri antigens on the immune mechanisms of rainbow trout (Oncorhynchus mykiss) were examined. The weight of the 120 fish used in this study was 20-30 g. After injecting 1 mg of formalin-inactivated whole cells (FKC) and O-antigen (Ag-O) intraperitoneally, blood was taken from the caudal vein of anaesthetized fis...

In the present study, effects of sulfamerazine on some haematological and immunological parameters of rainbow trout (Oncorhynchus mykiss) were examined. Four groups of rainbow trout were fed experimental diets containing either no sulfamerazine (control) or supplemented with sulfamerazine at 100 mg kg−1 (Exp-A), 200 mg kg−1 (Exp-B) or 400 mg kg−1 (...

zet Bu çalışmada, spektrofotometrik yöntemle Elazığ, Keban yöresinde yaşayan, Türk salyangozu olarak da bilinen Helix lucorum' da hepatopankreas, bağırsak ve kas dokusunda vitamin A ve β-karoten miktarları ile aylara göre değişimleri araştırıldı.Çalışmada kullanılan salyangozların hepatopankreasındaki en yüksek vitamin A düzeyi nisan ayında ortalam...

Elazığ, Keban ilçesinde bir gökkuşağı alabalığı (Oncorhynchus mykiss) üretim tesisinde anaç alabalıklarda ölümler belirlendi. Ölüm belirlenen havuzlardaki balıklar sistematik olarak balık hastalıkları yönünden incelendi. Yapılan araştırma sonucunda balıkların sindirim kanalında Eimeria truttae türü protozoona bağlı olarak koksidiyoz enfestasyonu te...

In this study, 236 marsh frogs Rana ridibunda collected from Lake Hazar (Elazig, Turkey) at 15 d intervals between March 2001 and February 2002 were examined for endohelminths; of these, 148 (62.71%) frogs were found to be infected with helminths. In total, 9 helminth species (3 trematodes, 5 nematodes and 1 acanthocephalan) were identified. We obs...

In this study, total 20 snails (Helix lucorum) obtained from Keban Region of Elazığ were examined interm of endohelmintes. At the end of parasitological examination of snails, Paragonimus sp. (Trematoda; Digenea) and Protostrogylus sp. (Nematoda; Metastrongyloidea) were determined in the intestine of snails. The abundance, prevalance and mean inten...

In this study, total 20 snails (Helix lucorum) obtained from Keban Region of Elazığ were examined interm of endohelmintes. At the end of parasitological examination of snails, Paragonimus sp. (Trematoda; Digenea) and Protostrogylus sp. (Nematoda; Metastrongyloidea) were determined in the intestine of snails. The abundance, prevalance and mean inten...

Deltamethrin, a synthetic pyrethroid pesticide contaminating aquatic ecosystems as a toxic pollutant, was investigated in the present study for acute toxicity on fry of rainbow trout, Oncorhynchus mykiss. Deltamethrin was applied at concentrations of 0.25, 0.5, 1, 2, 4, 6, 8, 10, 12, 25, 50 μg L−1. The experiments were repeated five times and a tot...

The purpose of this study was to determine the acute toxicity and effect of malathion on Daphnia magna which was used as test animals. Daphnids were exposed for 96 h to malathion concentrations ranging from 0.001-4 ppm and death daphnids were counted 24, 36, 48, 72 and 96 h later under a stero microscope. The values of lethal concentration (LC<SUB>...

In this study Daphnia magna was used as test animals. Daphnids were exposured for 96 h to DDVP concentrations ranging from 0.001-0.5 ppm. Death daphnids were counted under a stero microscope 24, 36, 48, 72 and 96 h intervals. The values of lethal concentration (LC<SUB>10, 50, 90</SUB>) and Lethal time (LT<SUB>10, 50, 90</SUB>) associated 95-99% con...

In this study, the acute toxicity of m-cresol on fry rainbow trout, Oncorhynchus mykiss, was investigated. Liquid m-cresol (C7H 8O) was applied at concentrations of 1, 2, 3, 4, 5, 6, 7, 8, 10, 16, and 32 ppm in 80-L aquariums with micropipette. Temperature, pH and dissolved oxygen of aquarium water were also measured and for each treatment, three r...

In this study, a dog with a large hematoma on the right side of its body after a blunt injury was brought to the Surgery Clinic of Veterinary Faculty in the University of Firat, Elazig. Five medicinal leeches (H medicinalis), obtained from Cip Fisheries Research Station of Fisheries Faculty, Firat University were placed on the hematoma. The leeches...

In this study, hematomas were induced with trauma to the arteries and veins of the external surface of the ears of five healthy adult dogs. The following day, three medicinal leeches (H. medicinalis) were attached to the area of the hematoma in unanesthetised dogs allowed them stay until they were full and self detached. This treatment was continue...

An outbreak has appeared affecting rainbow trout weighting 0.2 – 0.8 g from five different fish management units in East Anatolia Region between years 2000 – 2002. Infected fish had exophthalmus, and dark skin. They were calm, feed unwillingly and sometimes eat noting. Intestine of fish was empty and hemorrhagic. Flavobacterium psychrophilum was de...

Fifteen individuals of four marine fish species (Torpedo marmorata, Raja clavata, Scorpaena porcus and Scorpaena scrofa) caught in the Dardanelles, Turkey, were examined for the leech Pontobdella muricata and Trachelobdella lubrica; 18 P. muricata and 23 T. lubrica were found on gills, at the base of the fins, and abdomen on 14 of the 15 fish.

In the present study, the semi–intensive production of 10 snails (Helix lucorum) collected from Keban region (Elazığ) was carried out in the laboratory condition. The samples were placed on the sand in the aquariums in November 2002. The food and moisture requirement of the snails were provided daily. It was observed that snails laid egg in the beg...

This study investigated the effect of a therapeutic bath in 1/15000 (66.67 mg/L) and 1/200000 (5 mg/L) concentrations of malachite green on the values of selected haemotological indicators of rainbow trout, Oncorhyn-chus mykiss. During five days the fish were exposed to the above concentrations of malachite green for 30 seconds and 15 minutes, resp...

Tetrahymena pyriformis and Epistylis sp. found on the body surface, mouth and oesophagus of freshwater leech, Nephelopsis obscura were studied. Tetrahymena pyriformis was defined on the body surface, in the mouth and oesophagus of Nephelopsis obscura . But Epistylis sp. was only determined on the body surface of leech. The mean length of Tetrahymen...

The freshwater leech Helobdella stagnalis was found in fish ponds and discharge channels at a Fisheries Research Station near Elaziǧ on the Upper Euphrates. It was identified on the basis of morphological features. The species had not previously been recorded in Turkey. The abundance of the species was highest (1.3±0.07 leeches/m2) in August and lo...

The freshwater leech Helobdella stagnalis was found in fish ponds and discharge channels at a Fisheries Research Station near Elazığ on the Upper Euphrates. It was identified on the basis of morphological features. The species had not previously been recorded in Turkey. The abundance of the species was highest (1.3±0.07 leeches/m²) in August and lo...