Nadine M Richings

Nadine M Richings
Monash University (Australia) · Education Program in Reproduction and Development (EPRD), Department of Obstetrics and Gynaecology

PhD(Melb), BSc(hons)(La Trobe)

About

28
Publications
7,282
Reads
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314
Citations
Additional affiliations
March 2011 - present
enRICHed Pursuits
Position
  • Biologist | Educator | Science Communicator
Description
  • Biology Education specialising in Reproduction and Development, Assisted Reproductive Technology (ART)
July 2010 - present
University of Melbourne
Position
  • Teaching Associate
Description
  • Lecturer and Tutor for Sex, Science & Community
March 2010 - present
enRICHed Pursuits
Position
  • Consultant Biologist & Educator
Description
  • enRICHed Pursuits specialises in Biology Education & Training. enRICHed Pursuits: au.linkedin.com/pub/enriched-pursuits/32/962/42b/ Dr NRichings: au.linkedin.com/in/nadinerichings/
Education
October 1999 - June 2004
University of Melbourne
Field of study
  • Reproductive Biology
February 1988 - November 1988
La Trobe University
Field of study
  • Embryology
February 1984 - November 1987
La Trobe University
Field of study
  • Biological Sciences

Publications

Publications (28)
Article
Full-text available
Like other assisted reproductive technology (ART) procedures, the cost of egg freezing (EF) is significant, presenting a potential barrier to access. Given recent technological advancements and rising demand for EF, it is timely to reassess how EF is funded. An online cross-sectional survey was conducted in Victoria, Australia and was completed by...
Article
Background Since 2014, many companies have followed the lead of Apple and Facebook and now offer financial support to female employees to access egg freezing. Australian companies may soon make similar offers. Employer-sponsored egg freezing (ESEF) has raised concerns and there is academic debate about whether ESEF promotes reproductive autonomy or...
Article
STUDY QUESTION What are the cohort trends of women undergoing oocyte cryopreservation (OC)? SUMMARY ANSWER There has been a dramatic increase in OC cycles undertaken each year since 2010, and the demographics of women accessing OC has shifted to a younger age group, but so far very few women have returned to use their cryopreserved oocytes in trea...
Article
Full-text available
Egg freezing (EF) technology has improved significantly over the last decade, giving women more choice over their reproductive futures. Despite this advance, EF brings forth contentious ethical and regulatory issues. Policies controlling access to EF vary around the world and there is a lack of consensus about who should have access and what criter...
Article
Full-text available
This proceedings report presents the outcomes from an international Workshop designed to establish consensus on the professional status of the clinical embryologist, and then to work towards creating international standards that can be referenced by regulators and professional societies around the world. The participants represented a total of 20 c...
Conference Paper
Full-text available
A general overview of professional status, a summary of the professional activities in Clinical Embryology and an introduction to the major consensus points developed at the Alpha Consensus Meeting on the professional status of Clinical Embryologists: an expert meeting.
Article
Full-text available
The aim of this study was to develop methods for cryopreservation and long-term maintenance of putative bovine embryonic stem cells (ESCs). Putative bovine ESC (bESC) lines (n = 3) isolated in conventional medium were used to compare slow-freezing and vitrification. After warming, vitrified cells (96.9%) demonstrated significantly (P < 0.05) better...
Article
Pluripotent embryonic stem cells (ESCs) were first isolated nearly three decades ago from mice, yet efficient ESC isolation has been limited to rodents and primates to date. We report a novel and robust technique for isolating ESCs from mammalian pre-implantation embryos by altering the epigenotype of embryonic explants and using pressed zona pellu...
Article
Full-text available
Oestrogen has wide ranging effects in development mediated mainly via the two oestrogen receptors, alpha (ESR1, also known as ERalpha) and beta (ESR2, also known as ERbeta). Oestrogen is the key factor that directs the indifferent gonad to become an ovary in many non-mammalian vertebrates. Oestrogen is not required for early ovarian differentiation...
Article
Full-text available
To produce autologous ESCs for a bovine model of cell therapy, we activated oocytes by calcium ionophore (CI) and 6 dimethylaminopurine (6 DMAP) and isolated ESCs from the resulting parthenotes. Parthenote ESC lines (pbESC) would also provide a valuable tool for epigenetic studies on ESCs. Five pbESC like-cell lines were expanded for 12 passages ov...
Article
The aim of the present study was to conduct preliminary experiments towards generation of autologous human embryonic stem cell lines by somatic cell nuclear transfer (SCNT) and parthenogenesis. A total of fifteen MII human oocytes were obtained from six female donors from a collaborating IVF clinic. Each woman having produced more than 20 eggs, don...
Conference Paper
Full-text available
Bovine ESCs (bESCs) were repeatedly passaged to derive four cell lines, 2 from Control cultures (no 5-azacytidine) and 2 from Treatment cultures (5-azacytidine). The Treatment and Control ESCs had a similar morphology, with a high lipid content and low cytoplasm-to-nucleus volume. Rex1 and Oct4 transcripts were present at Day 29 (during Passage 3)...
Article
Full-text available
Cold storage is a simple method for storing and transporting tissues and organs. The reliability of this method for maintaining structure and function of marsupial ovarian tissue was assessed using histological techniques and follicle culture. Tammar wallaby ovaries were placed in cold storage (phosphate-buffered saline at 4 degrees C) for 24 or 48...
Conference Paper
Full-text available
Isolated preantral follicles from prepubertal and adult tammars were cultured in defined (serum-free) media and follicle growth, antrum formation and follicle quality were compared. FSH promoted follicle growth and antrum formation in follicles from prepubertal tammars. FSH promoted growth of follicles from adults, but other factors present in seru...
Article
Full-text available
Here we report the first use of intra-cytoplasmic sperm injection (ICSI) in a marsupial, the tammar wallaby (Macropus eugenii), to achieve in vitro fertilization and cleavage. A single epididymal spermatozoon was injected into the cytoplasm of each mature oocyte collected from Graafian follicles or from the oviduct within hours of ovulation. The da...
Article
Full-text available
A 'stunning' technique, combining a rifle shot with spotlighting and hand netting was used for the live-capture of southern hairy-nosed wombats. A successful stun was defined as a rifle shot that resulted in the temporary deafening and disorientation of a wombat. When combined with spotlighting, this technique enabled catchers with nets to approach...
Conference Paper
Introduction: Ovarian follicles from prepubertal animals are ideal for in vitro studies as they have had little extra-ovarian hormonal influence and are therefore like a blank template on which to assess defined culture conditions. Follicles from adult ovaries have been exposed to an indeterminate and varied hormonal environment over a number of ov...
Conference Paper
It is standard practice to use phosphate-buffered saline (PBS) stored at 4oC to transport excised gonads from the field to the laboratory for subsequent tissue culture. These storage conditions must temporarily or permanently affect the structure and function of the tissue and cells. The aim of this study was to assess the histological effects in o...
Conference Paper
The ovaries of female mammals contain many more oocytes than an animal will ovulate during her reproductive lifetime. Though the death of a female means the end of her reproductive output, it may be possible to salvage her reproductive potential by harvesting oocytes post-mortem. This is a particularly pertinent consideration for threatened species...
Article
Full-text available
Introduction: The culture and transfer of human blastocysts theoretically allows greater selection of potentially viable embryos than conventional IVF and embryo transfer. Success- ful blastocyst culture and transfer have been carried out using medium containing serum and/or co-cultured heterologous somatic cells. The above approaches can pose logi...
Article
Full-text available
To determine the success of intracytoplasmic sperm injection for severe male infertility. A retrospective survey. A tertiary infertility service. One hundred fourteen couples had 119 intracytoplasmic sperm injection treatments because of previous failure of standard IVF, poor results with subzonal insemination, sperm concentration < 2 x 10(6)/mL, o...
Article
OBJECTIVE: To determine the success of intracytoplasmic sperm injection for severe male infertility. DESIGN: A retrospective survey. SETTING: A tertiary infertility service. PATIENTS AND INTERVENTIONS: One hundred fourteen couples had 119 intracytoplasmic sperm injection treatments because of previous failure of standard IVF, poor results with subz...
Article
Full-text available
Sperm preparation for intracytoplasmic sperm injection (ICSI) is described and the effect of high speed centrifugation during preparation on fertilization rate is evaluated. No significant differences were found in the 2-pronuclear or abnormal fertilization rates between sibling oocytes injected with sperm prepared by swim-up or mini-Percoll combin...
Article
Full-text available
The outcome of treatment by intracytoplasmic sperm injection (ICSI) is described for patients with severe male infertility. In 296 consecutive cycles, a normal fertilization rate of 69% was achieved with 288 cycles (97%) resulting in embryos suitable for transfer. A total of 32 clinical pregnancies were achieved from the transfer of fresh embryos (...

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Projects

Projects (9)
Project
To develop and use Assisted Reproductive Technology (ART) and Biotechnology for the conservation of species. In particular, to use well-established methods such as gamete collection, insemination methods (both in vivo and in vitro), embryo culture, embryo transfer and cryopreservation of gametes, embryos and gonadal tissue. Also to develop and apply novel methods such as Stem Cell Technology.