Nadezhda Zholobak

• Ph.D in Virology
• Senior Researcher at National Academy of Sciences of Ukraine

Cerium oxide nanoparticles (CeNPs) represent a highly promising material for a number of chemical and biological applications involving oxidation-reduction processes. However, the impact of synthesis conditions, as well as the...

The present research is devoted to the investigation of a nanosystem including gold nanoparticles in an aqueous solution of sodium sulfacetamide in order to determine the nature and efficiency of their interaction depending on the concentration and pH of the medium. Sodium sulfacetamide is of interest due to the possibility of its use in the compos...

The functionalization of CeNP with the amino acid histidine can significantly change the antiviral effects of CeNPs, both pristine and containing different concentrations of Pt. The obtained results are a promising tool for the creation of effective complex functionalized and biocompatible CeNPs as targeted antiviral objects.

The described range of complex effects of CeNPs in virology indicates its multifaceted role in the implementation of the interaction between the virus and the sensitive object, which opens up prospects for its versatile application in the prevention, therapy, and diagnosis of viral infections.

Cerium oxide nanoparticles (CeNPs) show great promise in biomedical applications such as theranostics of various diseases, including viral infection. In this work, we synthesized bare, histidine- and glycine-functionalized CeNPs sols and characterized them using DLS, TEM and AFM imaging, synchrotron radiation-based techniques (XPS, RPES, NEXAFS). T...

В книге доступно, начиная с основ, без которых трудно понять сложные процессы, происходящие со временем в нашем организме, раскрыты метаболические процессы и функции, которые зависят от фундаментальных клеточных органелл-митохондрий. Авторы предлагают читателям ознакомиться с самыми последними достижениями биологических наук, представляя также собс...

“Apinormin” is a probiotic for bee bacterial diseases treatment based on Lactobacillus plantarum, Bifidobacterium bifidum, Enterococcus faecium. Colloidal (nanosized) cerium dioxide increases the activity of antimicrobials and is an agent for their delivery into cells and has bactericidal effect.Results of research and discussion. It was establishe...

Compounds based on cerium are highly promising objects in biotechnology regarding their high biological activities such as antiviral, antibacterial, antifungal, neuro- and radioprotective action, and antioxidant activity. On their basis is possible to develop compositions capable of activating the systems of cellular and humoral immune defence and...

We studied the toxic effects of cerium and fluoride species on human dental pulp stem cells and epithelial cells of Cercopithecus aethiops as a surrogate for the human oral mucosa. The sequential use of CeCl3 and NH4F solutions in equimolar sub-toxic concentrations enabled the possible toxic effects of individual components to be avoided, ensuring...

The aim of our investigation was to validate the expression of the heterologous HuINFalpha-2b gene in transgenic tomato lines

The effect of various concentrations (1 μM – 100 mM) of citrate-stabilized cerium dioxide nanoparticles or cerium ions (3+) on the biomass production of two species of unicellular green algae Desmodesmus armatus (Chod.) Hegew and Acutodesmus dimorphus (Turpin) Tsarenko was studied, the amount of chlorophyll, proteins and lipids in the algae biomass...

The study results of the interaction of bovine serum albumin (BSA) with cerium dioxide CeO2 nanoparticles in saline solution at different temperatures are presented. The static mechanism of fluorescence quenching of the solutions was established. The formation of BSA-CeO2 conjugates occurs under the predominant van der Waals forces and hydrogen bon...

Based on the results of physicochemical analysis, IR-spectroscopy, derivatographic analysis and differential scanning calorimetry, it was established that the interaction of cerium(III) carbonate with methanesulfonic acid yields cerium(III) methanesulfonate Се(SO3CH3)34H2O. Thermolysis of complex compound Се(SO3CH3)34H2O proceeds via a complex ch...

В книге рассмотрен широкий круг вопросов: роль митохондрий в эволюции животного и растительного царств, функциональные взаимодействия между органами и митохондриями, анализ типов метаболизма и роль радикалов кислорода в процессах старения человека. Автор сделал попытку максимально доступным способом изложить современное понимание биоэнергетических...

The work is devoted to the study of the influence of cerium in the form of salt and nanoparticles on the culture of gramnegative toxic freshwater cyanobacteria Microcystis aeruginosa (Kützing) Kützing. M. aeruginosa is a typical representative of the algae flora of temperate latitudes, capable of producing neuro- and hepatotoxins (microcystin and c...

Cerium dioxide nanoparticles (CeO2 NP) have significant catalytic and antioxidant properties due to their ability to inhibit the development of free-radical reactions due to a combination of superoxidedismutase and catalase mimetic activities. This nanopreparation has a significant antibacterial effect, but is low-toxic to unicellular yeast Sacchar...

Existing and new methods of technology development and production of prophylactic antiviral vaccines are considered, their disadvantages and advantages are analyzed. An analysis of the conditions when vaccination cannot protect against infectious diseases was carried out. The results of own research on the use of nanotechnology in the development o...

A water- and alcohol-soluble cerium oxide-curcumin conjugate was obtained by co-evaporation with poly(N-vinylpyrrolidone) (PVP). A nanocomposite consisting of hybrid organic-inorganic particles was stable in a wide range of pH values. Its properties were evaluated using nine cell lines: normal (MDBK, ST, Vero) and malignant (L929, T98G, HEp-2, A549...

Background Microbiome modulation is a pillar intervention to treat metabolic syndrome, prestages, and cascade of related pathologies such as atherosclerosis, among others. Lactobacillus and Bifidobacterium probiotic strains demonstrate efficacy to reduce obesity, dyslipidemia, and improve metabolic health. Novel prebiotic substances composed with k...

Cerium oxide nanoparticles (nanoceria) possess enormous biological activity, wherein many aspects related to the biological properties of nanoceria still remain unclear. In the present chapter, we have tried to explore the possible biological mechanisms of nanoceria action in terms of the catalytic activity of CeO2 particles and chemical behavior o...

Recently, nanobiotechnology has been developing intensively; therefore, various properties of nanoparticles, which depend on their origin, concentration, and size, are of interest. It is known that CeO2 nanoparticles cause a positive biological effect. These particles are able to penetrate through biomembranes. At the same time, there are assumptio...

Cerium oxide nanoparticles (CeONP) were used as the modifying agent for the recombinant tumor necrosis factor-α and recombinant tumornecrosis factor-α-thymosin-α1 (rhTNF and rhTNF-T). A notable increase of the biological activity of proteins with antitumor effect wasdemonstrated. It was established that the cytotoxicity of rTNF-T+CeONP composite in...

Background: Thrombotic disease is extremely harmful to human health, and early detection and treatment can improve the prognosis and reduce mortality. Platelet overactivation, resulting in excessive reactive oxygen species (ROS) production, hypercoagulation and growth factor secretion, plays a crucial role in thrombotic events and abnormal angiogen...

Introduction and Aims. Osteoclastogenesis is a complex process that plays a critical role in bone remodeling. Based on detailed knowledge of the molecular mechanisms involved in osteoclastogenesis, new pharmacological agents (including nanoparticles) that selectively influence the differentiation or the activity of bone cells were developed during...

Background: While antiviral and interferon-inducing agent tilorone is used as a reactant of a number of popular pharmacological preparations, the molecular mechanisms of its biological antiviral activity are under discussions among the specialists. That is why the molecular level model studies of interactions of tilorone with targeting biomolecules...

The aim was to study the effects of nanoceria on pro / antioxidant balance in the tissues of salivary glands in glutamate (MSG)-induced obese rats. We included 48 Wistar rats of both genders and divided into four groups, and conducted comparative assessment of intragastric administration of nanocrystalline cerium dioxide at a dose of 1 mg/kg at the...

The article addresses the influence of cerium dioxide nanoparticles (CDN) on the cytokines production in terms of their application in the composition with interferon (IFN), and as an independent drug. The application of the CDN in combination with IFN showed an increased interferon response of experimental mice compared to unmodified IFN. IFN-CDN...

We synthesised a new type of photochromic tungsten oxide nanoparticles, analysed their photocatalytic activity and carried out a thorough analysis of their effect on prokaryotic and eukaryotic organisms. Ultrasmall hydrated tungsten oxide nanoparticles were prepared by means of hydrothermal treatment of tungstic acid in the presence of polyvinylpyr...

Ceria nanoparticles are capable of performing the function of some enzymes (such as oxidoreductases, phosphatase) and can be classified asnanozymes. In this review, the actual data on the enzymatic activity of ceria were critically analyzed and specific conditions under which thecerium dioxide nanoparticles can act as enzymes were defined. The pres...

In the research, we were used normal cells that were received ex vivo, and transfected cell lines to establish the toxicity of non-stabilized and citrate-stabilized nanoparticles of cerium dioxide and to study their influence on the nitrite ion production. As a result, there was established the most effective concentrations of different type cerium...

Background Nowadays, we face the global epidemic of obesity, that is known to contribute to the development of many diseases, such as the oral cavity pathologies. Dental and oral pathologies are frequently caused by and overlapped with systemic multifactorial diseases such as obesity being its early indicators and risk factors.The aim was to study...

In the last decade, ferromagnetic nanoparticles that are able to be heated under an AMF (alternating magnetic field) have gained considerable interest in the field of nanotechnology. The current study explores the peculiarity of the synthesis and the properties of Fe3O4 and (La, Sr)MnO3 nanoparticles by cryochemical and sol-gel technology, as well...

Additional pictures and experimental data.

The effect of illumination on the infectivity of the system 'virus-nanoparticle' by the example of Vesicular stomatitis Indiana virus with gold nanoparticles has been studied both theoretically and experimentally. Theoretical consideration is based on the ideas of nanooptics and applies a model of the interaction in the system 'mesoparticle-quantum...

Luminescent organic dots (O-dots) were synthesized via the one-pot, solvent-free thermolysis of citric acid in the urea melt. The influence of the precursors’ ratio and the duration of process on their properties were established and the mechanism of their formation was hypothesized. The multicoloured luminescence tunability and toxicity of synthes...

Introduction. Tilorone (amixin, dihydrochloride 2,7-bis[2-(diethylamino)ethoxy] fluorenone-9) is a low molecular interferon inducer that is effective against a wide range of viral infections including herpes viruses. The mechanism of tilorone antiviral action is associated with inhibition of virus-specific peptides translation in infected cells res...

Interferons (IFNs) are cytokines produced and secreted by virtually all cells of vertebrates in response to stimulation by certain viruses, bacteria, antigens, and mitogens; they mediate their biological activity by binding to specific receptors on the cell surface [1]. Due to prospect and active using the recombinant interferon alpha-2β (rIFN α-2β...

The ratio between omega-3 and omega-6 fatty acids (FAs) in organism's tissues may indicate whether it undergoes inflammation because they are the precursors of oppositely acting classes of eicosanoids regulating the immune response. Previous studies showed the change in RBC fatty acids composition during chronic HSV-1 infection in mice and human, h...

Nanoparticles of cerium dioxide (NCD) is a perspective material in antiviral therapy. It is shown that nanocrystal cerium dioxide has antiviral activity against Herpes simplex virus 1 (HSV-1) in RF cell line and Vesicular stomatitis Indiana virus (VSV) in L929 cell line. Citrate-stabilized NCD inhibits nitrite production in MDBK cell line before an...

Plurality and individuality of hepatocellular carcinoma: PPPM perspectives Krishna Chander Sridhar, Olga Golubnitschaja Department of Radiology, Univeristy of Bonn, Bonn, Germany Correspondence: Olga Golubnitschaja (olga.golubnitschaja@ukb.uni-bonn.de) – Department of Radiology, Univeristy of Bonn, Bonn, Germany The EPMA Journal 2016, 7(Suppl 1)...

In this paper, we summarize the current data on biosecurity of nanocrystalline cerium dioxide (CeO2 NP) for environmental microorganisms, and on the probiotic effect of CeO2 NP. We analyze the possible ways of the interaction of nanoparticles with the bacterial cell, which provide the means of their probiotic and antibacterial action. The analysis...

Zabolotny Institute of microbiology and virology of the NAS of Ukraine. Department of interferon and immunomodulators. The innovative developments include the establishment of nanomaterials biological activity: nanoparticles of gold and silver, nanocrystalline cerium dioxide (nanoceria) for viral infections and other pathological conditions, in par...

Tumor necrosis factor-alpha (TNF-ɑ) is important cytokine which functions include induction of apoptosis, cytolysis or cytostasis of tumor cells, activation of polymorphonuclear leukocytes and induction of IL-1 or colony stimulating factor expression [1]. TNF-ɑ also has antiviral activity and involved in antiviral response. Thus, astrocytes infecte...

The effect of nanoceria (CND), that have different physical and chemical properties – the used producing methods, Zeta potential, the presence of the stabilizer – on an intensity of luminescence of Photobacterium phosphoreum IMV B-7071 was studied. It is shown that the high crystallinity nanoceria after 30 minutes after addition stimulated the cell...

Ammonium hexanitrocerate (IV) aqueous solution is prepared to produce stable aqueous colloidal solutions of cerium dioxide nanoparticles, by intimately stirring it until complete dissolution. Hydrothermal treatment of the solution is carried out at a temperature of 80-200°C during 0.4–50 hours. The precipitate of cerium dioxide nanoparticles is sep...

Nanocrystalline ceria (nanoceria) is a multifunctional material which shows promise for use in biomedical applications. In particular, CeO2 nanoparticles can interact with bacteria both as an antimicrobial agent and as a probiotic agent. This survey compiles the existing data on the influence of nanoceria on bacteria, including clinically important...

This article analyzes various perceptions about the modern-day antiviral therapy. It is shown that the total list of drugs used to treat viral infections contains only 20% of the antiviral medications. The article considers the prospects the combination therapy, which takes into account both the specifics of the various stages of the virus-cell int...

Background. The development of new drugs with antiviral activity and the ability to induce interferon is an urgent assignment, taking into account the rapid spread of viral infections, development of resistance the virus strains to survive antiviral drugs and the overall decline of the immune response. Objective. Determine the antiviral activity an...

We have demonstrated the influence of cerium dioxide nanoparticles on the immunogenicity of the influenza vaccine on an example of liquid split inactivated Vaxigrip vaccine. Antibody titers were analyzed using the hemagglutination inhibition (HI) assay. Seroprotection, seroconversion, the geometric mean titers (GMTs) and the factor increase (FI) in...

Here we describe the most important preparation strategies and physical and chemical properties of ceria nanoparticles (nanosized ceria, or nanoceria). The main focus of this survey is on the therapeutic potential of nanoceria in age-related pathologies. We also highlight the impacts of nanoceria on living systems, specifically on the progression o...

This work is the first to our knowledge to describe the successful attempt of Agrobacterium rhizogenes-mediated transformation of topinambour in order to obtain the transgenic H. tuberosus plants, callus and “hairy” root cultures. The plasmid vectors contained the sequence of interferon gene fused with Nicotiana plumbaginifolia L. calreticulin apop...

We propose a new method of purifying biological liquids from viruses, based on the effect of enhancement of the Casimir force between a nanoparticle and a surface when the latter carries a propagating surface plasmon-polariton. It is supposed that when the virus is located close to the surface, its receptors could be deformed or destroyed due to th...

Aim. To investigate changes in the pro-inflammatory status of laboratory rats after the introduction of antiviral substances with interferon-inducing action: 6-(2-morpholin-4-yl-ethyl)-6H-indolo[2,3-b]quinoxaline and tilorone. Methods. MCP-1 content, blood leukocyte counts and functional activity of phagocytes was measured using flow cytometry tech...

The article provides an overview of current scientific data on the antibacterial effects of perspective biotechnological material – nanocrystalline cerium dioxide (CeO2 NP). It was shown that in contrast to micro objects nanoscale particles of cerium dioxide have significant antibacterial activity, which depends on the surface charge of the particl...

It was established that with age the content of lipid peroxidation products increased in the mucosa of the stomach: Diene conjugates by 30%, products which react to thiobarbituric acid by 285% and Schif bases by 181%. Nanodisperse cerium dioxide (NCD) reduced the content of lipid peroxidation in the gastric mucosa in old rats: Diene conjugates by 4...

Male infertility has largely idiopathic, multifactorial origin. Oxidative stress is a major factor that affects spermatogenesis, in particular in aging. Cerium dioxide nanoparticles (CNPs) due to their antioxidative properties are promising to impact on the development of male infertility. The aims of this study were to investigate the effects of C...

An increase of the number and the variety of anthropogenic sources of radio-frequency electromagnetic fields (RF-EMFs) causes a high interest in studying the effects of non-ionizing radio waves on living organisms. Amongst many biological targets, the DNA molecule has received the greatest attention with respect to potential RF-EMFs damage. The aim...

A series of novel 6-(2-aminoethyl)-1,2,3,4-tetrahydroindolo[2,3-b]quinoxalines (3a–f) was synthesized. Members of this series were designed as nonplanar analogs of 6-(2-aminoethyl)-6H-indolo[2,3-b]quinoxalines (1a–l). Compounds 1a–l have recently been reported to possess a high antiviral activity, interferon-inducing properties and low cytotoxicity...

It was established that with age the content of lipid peroxidation products increased in the mucosa of the stomach: Diene conjugates by 30%, products which react to thiobarbituric acid by 285% and Schiff bases by 181%. Nanodisperse cerium dioxide (NCD) reduced the content of lipid peroxidation in the gastric mucosa in old rats: Diene conjugates by...

Constipation is a common condition, with prevalence after 65 years, is a major colorectal cancer risk factor. Recent works have demonstrated advances in personalized, preventive nanomedicine, leading to the construction of new materials and nanodrugs, in particular, nanocrystalline cerium dioxide (NCD), having strong antioxidative prebiotic effect....

A novel facile method of non-doped and fluorescent terbium-doped cerium fluoride stable aqueous sols synthesis is proposed. Intense green luminescence of CeF3:Tb nanoparticles can be used to visualize these nanoparticles' accumulation in cells using confocal laser scanning microscopy. Cerium fluoride nanoparticles are shown for the first time to pr...

This review summarizes available information on the application of nanocrystalline ceria in cancer therapy and diagnostics (theranostics). Factors responsible for the high biological activity of nanocrystalline ceria are analyzed. Principles of design of multifunctional drugs based on CeO2 are considered, and examples of their practical use are pre...

Despite the high efficiency of the etiotropic antiviral drugs and antiviral vaccines they can lead to the selection of resistant mutant variants, initiate of evolutionary "arms race" [1] and pose a threat of the superinfections and epidemics. In this regard, the creation of means that enhance non-specific antiviral resistance is actual task and wil...

In experiments in vivo and in vitro, we investigated the effect of cerium oxide nanoparticles on the stability of red blood cells of old rats. The analysis of the kinetic curves of acid hemolysis caused by the penetration of protons into the matrix of erythrocytes in experiments in vivo showed a significant reduction in the so-called stability inde...

A method of direct monitoring of reactive oxygen species (ROS) interaction with cerium dioxide nanoparticles (CDN) in living cells is proposed based on the use of a calcein (CLC) and CDN complex. The CLC-CDN complex penetrates easily into a living cell and is decomposed readily by endogenous or exogenous ROS, releasing brightly fluorescent calcein,...

Механізм реалізації противірусної активності (ПА) та індукції інтерферону (ІФН) планарними поліциклічними сполуками досі не встановлено, хоча висунута нами гіпотеза про пріоритетну роль інтеркаляції в двоспіральні нуклеїнові кислоти (НК) набула вагомих аргументів на свою користь в наших роботах та роботах іноземних колег. З іншого боку, наявність П...

Antiviral effect of the molecular composition (MC) yeast RNA-tilorone and 2,7-bis[2-(diethylamino) ethoxy]-fluoren-9-on dihydrochloride (tilorone) was demonstrated on the experimental model of herpes infection in vivo. The reduction of mortality by 80 % was observed in the group of herpes-infected animals treated with the MC, and the average life e...

We investigated the effect of nanocrystalline cerium dioxide on parameters of spontaneous and stimulated motility of the stomach and colon in rats. It was found that administration of nanocrystalline cerium dioxide for 10 days increased the amplitude of contractions of stimulated motility in the stomach by 33.0 +/- 2.4% and the frequency of contrac...

The implementation mechanism of the antiviral activity (AA) and interferon induction (IFI) by planar polycyclic compounds has not yet been determined. However, our hypothesis of the priority role of intercalation in double strand nucleic acids (NA) has gained strong arguments in its favour in our works and the works of foreign colleagues. On the ot...

The effect of various concentrations of cerium dioxide nanoparticles and complexes on its basis on the growth of certain strains of phytopathogenic and symbiotic bacteria has been investigated. It is shown that the 1 mM and 10 mM concentration of cerium dioxide nanoparticles or complexes of CeO2 and exometabolites of Bacillus thuringiensis are the...