Nadezhda P Pal'mina

• PhD
• Professor (Full) at Russian Academy of Sciences

A liposomal formulation comprising a combination of hydrophobic nutraceuticals (omega-3 docosa-hexaenoic polyunsaturated fatty acid (DHA) and one of the most active plant antioxidants, namely clove essential oil (CEO)) was prepared using soy phosphatidylcholine (PC). The impact of DHA and CEO on the microviscosity of the bilayer of PC liposomes was...

Essential polyunsaturated fatty acids (PUFAs) of the n-3 and n-6 classes are crucial for maintaining many physiological functions of the human body. It has previously been suggested that the beneficial...

The aim of this study was to gain a better understanding of the key structural factors and intermolecular interactions underlying the formation, functionality, and in vitro gastrointestinal behaviour of the...

A water-soluble liposomal form was successfully developed based on phosphatidylcholine liposomes (PC) loaded by a combination of curcumin (CUR) with a balanced amount of n-3 and n-6 polyunsaturated fatty acids (PUFAs), which were encapsulated in an electrostatic complex of whey protein isolate (WPI) with chitosan [WPI−(PC−FO−CUR)]−CHIT. The encapsu...

The aim of this work was to elucidate the influence of both the structural features of milk proteins [whey proteins (WPI) and sodium caseinate (SC)] and the character of their electrostatic associative interactions with chitosan (CHIT) on the structure, thermodynamic parameters and functionality of their complexes with essential lipids. These lipid...

The aim of this work was to establish the main relationship between the structure and functionality of supramolecular complexes formed by sodium caseinate (SC) with phosphatidylcholine (PC) liposomes filled by...

Food grade biopolymers (whey protein isolate (WPI) and chitosan (CHIT)) effectively encapsulated phosphatidylcholine (PC) liposomes loaded with a combination of hydrophobic and hydrophilic nutraceuticals. The obtained biopolymer delivery systems provided the stability of nutraceuticals and their controlled bioavailability in the gastrointestinal tr...

We studied the physico-chemical properties of lipids of the most important organs (the liver and brain) of mice that received nanoliposomal complexes of different compositions in a long-term diet (3 months). The components of the nanoliposomal complexes included soy phosphatidylcholine, clove essential oil, fish oil, and sodium caseinate. We prepar...

In recent years, natural food hydrocolloids (proteins and polysaccharides) have been intensively researched for their application in improving health or preventing disease through dietary therapy. This review focuses on recently achieved insights into the essential contributions of the food hydrocolloids to both the formation of micro/nanoparticles...

Изучены физико-химические свойства липидов важнейших органов мышей (печени и головного мозга) после длительного приема в течение трех месяцев нанолипосомальных комплексов, состоящих из различных вариаций следующих веществ: фосфатидилхолина сои, эфирного масла гвоздики, рыбьего жира и казеината натрия. Из суммарных липидов органов готовили липосомы...

Much attention is given to research and development of efficient systems for the delivery of essential omega-3-polyunsaturated fatty acids and other nutraceuticals to the human body with food. Nanocomplexes, which are based on soybean phosphatidylcholine liposomes with nutraceuticals included, are among the efficient delivery systems. The prolonged...

The aim of this work is the clarification of the impact of the character of associative interactions (covalent and non-covalent (electrostatic)) between chitosan (CHI) and whey protein isolate (WPI) on the structure, thermodynamic parameters and functionality of their complexes with essential lipids. These lipids included soybean phosphatidylcholin...

A water-soluble and nanoscale peroral system has been obtained for the delivery of essential lipids. It was based on the supramolecular complexes of the lipids with biopolymers. This system provides both the high protectionof lipids against oxidation during storage and the ease addition of their adequate amounts into various liquid prophylactic age...

Design and research of liposome structures on the basis of soy phosphatidylcholine (PC) as nanocontainers for delivery of various functional nutraceuticals in tissues and cells of organisms, as well as models for studying biochemical processes in cell membranes, has been of undoubted interest recently. As liposomes are oxidized and destroyed under...

Разнообразные виды рода Aloe, которых в мире насчитывается более 500, считаются важными источниками биологически активных веществ и привлекают внимание исследователей многочисленными проявлениями их биологических свойств. Наиболее изученными и используемыми видами Aloe являются A. arborescens и A. vera, однако некоторые другие виды проявляют не мен...

Electron spin resonance (ESR) and atomic force microscopy (AFM) were used to study liposomes that were prepared from soybean phosphatidylcholine (PC); they incorporated plant antioxidants (ginger, allspice, and black-pepper extracts; clove oil; etc.) that were encapsulated in biopolymers (sodium caseinate or sodium caseinate–maltodextrin covalent c...

The relationship between rearrangement of the dispersed phase inducing considerable changes in the pH and nonmonotonic concentration dependences of membrane effects in aqueous systems of the endogenous regulatory peptide, thyroliberin (thyrotropin-releasing hormone), in 10–3–10–16 mol/L concentration range was demonstrated for the first time. The m...

Two concentration ranges (from 10−5 to 10−9 and from 10−13 to 10−18 M) corresponding to enhanced fluctuations of Rayleigh and Raman scattering of second-harmonic (527 nm) pulses of YVO4:Nd3+ laser are found for aqueous solutions of antioxidant potassium phenosan. A correlation is revealed between the rise in elastic Rayleigh scattering intensity an...

A number of structural (the weight-average molar weight, Mw; the radius of gyration, RG; the hydrodynamic radius, Rh; the structure-sensitive parameter, ρ=RG/Rh; the density, d; the intrinsic viscosity, [η]; the ζ-potential), and thermodynamic (the second virial coefficient, A2, reflecting the nature and intensity of both the biopolymer-biopolymer...

A nonmonotonic bimodal dependence of elastic, Rayleigh scattering on the potassium phenosan concentration in an aqueous solution has been revealed. A similar dependence has been found, for the first time, for scattering amplitude fluctuations. The observed correlation is indicative of structural transitions in hydration shells of potassium phenosan...

The development of smart (ie, stimuli-sensitive) nanosized natural delivery systems for essential bioactive compounds has attracted particular interest under the functional food formulation. The natural lipids (triacylglycerols and phospholipids), involving the essential ω -3 and ω -6 polyunsaturated fatty acids, are among the most important nutrac...

Abstracts Diluted water solutions of anti-oxidant potassium phenosan, kept before explorations in "usual" conditions and in conditions of "permalloy container", i.e. shielding of solution from the influence of external low-frequency electromagnetic and/or geomagnetic fields, were studied. It is shown that in solutions kept in shielded conditions in...

There are a number of aqueous-soluble high-molecular compounds, the biochemical activity of which has a nonmonotonic character in dependence on the concentration. It should be noted that the DLS provides the measurement of the radius of correlations of fluctuations of the refractive index of a substance, which coincides in the case of suspensions o...

The article presents data on changes in physicochemical properties of different biological membranes (plasmatic, microsomal, synaptosomes) under the action of biologically active substances, which are different in their chemical structure and the mechanism of action (natural and synthetic antioxidants, thyrotropin - releasing hormone, phorbol ester...

This paper discloses for the first time the effects of the gas phase (GP) and the tar of cigarette smoke on lipid peroxidation (LPO) and on the structure of different lipid regions in liposomes. The LPO development was analysed in terms of the total unsaturation of lipids (double-bond, DB, content) and the formation of dienic conjugates (DC), ketod...

The effects of 1,2-diphenyl-1,3-oxazole (DPhO) and its derivative - iod-methylate (IM-DPhO) in the concentration range of 10-2 -10-13M were studied on the dynamic lipid structure of microsomal membranes isolated from the liver cells of Balb mice in vitro. By method of electron paramagnetic resonance (EPR) using two spin probes 5-and 16-doxylstearic...

The effect of synthetic anti-oxidant potassium phenosan (PP, potassium salt of beta-(4-hydroxy-3,5-ditretbutil-phenyl)-propionic acid) on the structural state of the surface (8 angstroms) and deep (20-22 angstroms) lipid regions of plasma membranes of mice liver cells was studied by spin probes method in vitro in a wide range of concentrations (1(-...

The effect of synthetic anti-oxidant potassium phenosan (PP, potassium salt of β-(4-hydroxy-3,5-ditretbutil-phenyl)-propionic acid) on the structural state of the surface (8 Å) and deep (20–22 Å) lipid regions of plasma membranes of mice liver cells was studied by spin probes method in vitro in a wide range of concentrations (10−5–10−21 M). Two sta...

We have recently shown by spin probe ESR that synthetic antioxidant potassium β�(4 �hydroxy�3,5�di� tertbutylphenyl)propionate (potassium phenosan, PP) can modify the structural state of surface (8 A) and deeplying (20-22 A) regions of lipids of plasmatic membranes isolated from mouse liver cells in a wide concentration range (10 ⎯5 ⎯10 -21 M) in v...

2,5-diphenyl-l,5-oxazole (DPhO) is a well known component of scintillation liquid for measuring of radioactivity [1, 2], fluorescence and chemiluminescence detection of oxazole-labeled amines and thiols [3, 4], DPhO was used as probe to determine a level of mono-oxygenation in liver as well [5], Moreover, the oxazoles showed a number of biochemical...

Earlier, we demonstrated for the first time that the natural antioxidant α�tocopherol in a wide concentra� tion range can modify the structure of different lipid regions in the endoplasmic reticulum and plasma membranes of hepatocytes in experiments in vitro (1, 2). Despite the biochemical and functional differences between endoplasmic reticulum an...

α-Tocopherol (α-TP) is an effective natural antioxidant and important component of biological membranes localized in the lipid bilayer and capable of changing their structural dynamic state. . For this reason, it was of interest to study α-TP effect in a wide range of concentrations (10-25 M - 10-4 M) on viscosity parameters and thermally-induced s...

A new class of substances exhibiting radioprotective and radiosensitizing effects depending on the concentration of the substance has been found. The radioprotective effect is probably due to the resonant absorption of radiation energy and its transformation into low-energy forms, as well as reactions with water radiolysis products. We studied the...

Combined use of the data from several physico� chemical methods and subsequent analysis of the con� centration dependences of the parameters of associ� ates, physicochemical properties and reactivity of sys� tems containing low (in particular, picomolar) and

The effect of the natural antioxidant α-tocopherol in a broad concentration range (10−4–10−25 M) on the viscosity characteristics and thermally induced structural transitions of a lipid bilayer of plasma membranes of murine hepatocytes in vitro has been studied. Changes in the rigidity of surface (∼8 Å) and microviscosity of the deep-lying regions...

The effect of the natural antioxidant alpha-tocopherol in a broad concentration range (10(-4) - 10(-25) M) on the viscosity characteristics and thermally induced structural transitions of a lipid bilayer of plasma membranes of murine hepatocytes in vitro has been studied. Changes in the rigidity of surface (approximately Abb) of the lipid bilayer w...

The effect of 1,2-diphenyl-1,3-oxazole (DPhO) and its derivative-iod-methylate (IM-DPhO) in the concentration range of 10-2-10-13M were studied in vitro on the microviscosity (τ) and order parameter (S) of membrane lipids of microsomes isolated from the liver cells of Balb mice. By EPR-technique using of spin probes 5-and 16-doxyl-stearic acids (5-...

α-Tocopherol (α-TP) is an effective natural antioxidant and important component of biological membranes localized in the lipid bilayer and capable of changing their structural dynamic state. . For this reason, it was of interest to study α-TP effect in a wide range of concentrations (10-25 M-10-4 M) on viscosity parameters and thermally-induced str...

The effect of a natural antioxidant α-tocopherol (α-TL) (in concentrations from 10-2 to 10-17 M) on the activity of protein kinase C (PKC) isolated from rabbit hearts was studied. Subsequent modelling was performed in terms of kinetic methods. It was shown that α-TL inhibits PKC to a maximum of 80% by a non-competing mechanism. It was found that th...

In the past two decades, the problem of the effect of water solutions of biologically active compounds (hor� mones, peptides, pesticides, poisons, antioxidants, etc.) at ultralow concentrations on biological systems at different levels of organization—from macromole� cules, organs and tissues to animals, entire organisms, and even populations [1–4]...

The spin probe method was employed to study in vitro the effect of regulatory peptide thyroliberin on structural state of surface (0.8 nm) and deep (2 nm) lipid layers of the plasma membranes in mouse liver and brain. Thyroliberin in a concentration range of 10−3–10−18 M enhanced structural order of surface lipids, the maximum effect was observed a...

The effect of alpha-tocopherol (alpha-tp) prepared in solvents of different polarity in a wide range of concentrations (10(-4) M - 10(-25) M) on lipid phase structural characteristics of microsomal membranes isolated from mouse liver cells has been investigated in vitro. Structural changes in membranes were detected on a Bruker-200D ESR-spectromete...

Thyroliberin, or thyreotropin-releasing hormone (TRH), can modulate cell activity at ultra low concentrations (from 10-10 M to 10 -16 M). In this study, we used electron spin resonance method (ESR) to explore the effects of TRH in a wide range of concentrations (between 10 -4 M and 10-18 M) on thermo-induced structural transitions and microviscosit...

Pin2 and Oxki1 are cationic amphipathic peptides that permeate lipid membranes through formation of pores. Their mechanism of binding to phosphocholine (PC) membranes differs. Spin-probe experiments showed that both Pin2 and Oxki1 penetrate the lipid membrane of small unilamellar vesicles (SUVs). Moreover, the leakage of calcein and dextrans from P...

A role of the solvent polarity was investigated on example of effect of α-tocopherol in the wide concentration range including 'imaginable' dilution on viscosity properties of microsomal membranes in mice liver cells in vitro. In the region of 'physiological' concentrations and super-low doses both in polar and nonpolar solvents the phase character...

The action of 12-O-tetradecanoyl-13-acetate (TPA) in vitro in a wide range of concentration from 10(-3) mol/l down to ultra-low doses 10(-23) mol/l and dilution 10(-24) mol/l on the microsome membranes isolated from tumor--Ehrlich ascite carcinoma (EAC) has been studied by ESR-method using two spin probes: 5- and 16-doxyl stearates (5- and 16-DS) l...

The aim of the present work was to study by ESR-spin-probe technique the effect of the natural antioxidant alpha-tocopherol (alpha-tp) in vitro on the structural parameters (microviscosity, order parameter) of endoplasmic reticulum membranes of the mice liver cells starting from the concentration of 10(-3) mol/l and down to the dilution of 10(-25)...

On the basis of antioxidant (alpha-tocopherol and phenosan potassium salt) and peptide (thyroliberin) effects on the lipid peroxide oxidation (LPO) and lipid structural parameters of the endoplasmic reticulum membranes in wide concentration range (10(-20)-10(-4) mol/l) in vitro the possibility concerning a proposed role of "super-affine" receptors...

Thyroliberin (TRH) influence on microviscosity and thermoinduced structural transitions of biological membranes has been studied using spin probes and ESR technique. It was shown that TRH in three investigated concentrations (10(-6), 10(-10) and 10(-16) mol/l) in vivo resulted in increasing of the lipid microviscosity in the hydrophobic areas (20 A...

Peptides are known to have the ability of modulating the activity of important regulatory cellular systems. One of them--thyroliberin, i.e. thyreotropin-releasing hormone (TRH), causes changes in the membrane structure and morphology of rat erythrocytes, as well as activates retractive activity of lymphatic vessels in ultra low concentrations (10(-...

Synthetic antioxidant potassium phenosan in ultralow doses administrated in combination with antitumor antibiotic adriamycin in a therapeutic dose (8 mg/kg) markedly prolonged the mean life span of tumor-bearing animals compared to adriamycin monotherapy. This effect depended on the dose of antioxidant and was maximum at phenosan concentrations of...

Acetylcholinesterase (ACE) activity and lipid peroxidation (LPO) parameters were measured in the blood of patients with Alzheimer's disease (AD) during treatment with amiridine and gliatiline. Treatment was accompanied by inhibition of ACE. There was a statistically significant relationship between clinical efficacy and changes in ACE activity. AD...

Effects of the regulatory peptide thyroliberin on microviscosity of lipid components of endoplasmic reticulum biological membranes in mouse hepatocytes were studied by electron paramagnetic resonance. Thyroliberin in a concentration of 10(-3)-10(-18) M decreased microviscosity of surface layers of membrane lipids. This decrease was the most pronoun...

Acetylcholinesterase (AChE) activity and parameters of the system of regulation of lipid peroxidation (LPO) were estimated in blood of patients with Alzheimer's disease (AD) during therapy with amiridine and gliatiline. It was found that the therapy was accompanied by inhibition of AChE activity. A significant correlation was observed between clini...

Acetylcholinesterase (AChE) activity and parameters of the system of regulation of lipid peroxidation (LPO) were estimated in blood of patients with Altzheimer's disease (AD) during therapy with amiridine and gliatiline. It was found that the therapy was accompanied by inhibition of AChE activity. A significant correlation was observed between clin...

Kinetic parameters of spontaneous lipid peroxidation (LP) in endoplasmic reticulum (ER) membranes isolated from the liver of healthy mice, tumour-host and ascitic Ehrlich tumour cells have been studied. The LP kinetics was characterized based on the primary LP products, dienic conjugates (DC), and the extent of lipid substrate unsaturation (double...

The effects of natural lipid-soluble antioxidant, alpha-tocopherol (alpha-TP), and the synthetic water-soluble antioxidant, phenosan potassium salt (Ph-K), in a broad range of concentrations down to ultralow doses (10(-4)-10(-20) M) on the activity of protein kinase C (PKC) have been studied. It was shown that alpha-TP is a potent inhibitor of the...

Effects of 12-O-tetradecanoylphorbol-13-acetate (TPA) and protein kinase C (PKC) in a broad range of concentrations (10-18 - 10-7 M) on the lipid peroxidation (LPO) in the rat brain plasma membranes were studied. It was shown that TPA and PKC inhibit LPO; the concentration dependence curves have two maxima at 10-15 M and 10-12 M for TPA and at 10-1...

We studied the effects of 12-O-tetradecanoylphorbol-13-acetate (TPA) and protein kinase C (PKC) in a broad range of concentrations (10(-18)-10(-7) M) on lipid peroxidation (LP) in rat brain plasma membranes. TPA and PKC were shown to inhibit LP, the concentration curves had two maxima at 10(-15) M and 10(-12) M for TPA and at 10(-16) M and 10(-13)...