Nadezhda Dmitrievna Goncharova

Nadezhda Dmitrievna Goncharova
Russian Academy of Medical Sciences | RAMS · Research Institute of Medical Primatology, Sochi, Russia

Sci. D. (pathophysiology), PhD

About

81
Publications
5,342
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583
Citations
Additional affiliations
January 2013 - present
Federal State Budgetary Scientific Institution "Research Institute of Medical Primatology"
Position
  • Head of Endocrinology Lab
January 1993 - present
Institute of Medical Primatology or Research Institute of Medical Primatology of Russian Academy of Medical Sciences
Position
  • Russian Academy of Medical Sciences, Institute of Medical Primatology
Description
  • Endocrinology of Aging, Stress; Primatology; Hormones and Antioxidant enzyme defence
January 1993 - present
Russian Academy of Medical Sciences, Research Institute of Medical Primatology, Russia, Sochi
Position
  • Head of Department
Education
September 1968 - July 1973

Publications

Publications (81)
Article
We studied the effect of constant illumination on the effects of administration of arginine vasopressin (AVP), one of the most important regulators of the key adaptive hypothalamic—pituitary—adrenal (HPA) axis under basal conditions and during stress, as well as on the circadian rhythm of activity of HPA axis and the pineal gland in laboratory prim...
Article
With aging, incidence of severe stress‐related diseases increases. However, mechanisms, underlying individual vulnerability to stress and age‐related diseases are not clear. The goal of this review is to analyze finding from the recent literature on age‐related characteristics of the hypothalamic‐pituitary‐adrenal (HPA) axis associated with stress...
Article
Full-text available
An increasing number of studies are devoted to the study of the relationship of mental disorders, such as depression and anxiety, with the risk of developing type 2 diabetes mellitus. However, in the literature there are practically no publications on the study of the relationship of the features of higher nervous activity, in particular, adaptive...
Article
Full-text available
The study of the mechanisms underlying the increased vulnerability of the individual to stressful environmental factors in different age periods is of great relevance for prevention and effective treatment of stress-dependent diseases that are widespread in the population of aging individuals. The purpose of our study was to investigate the individ...
Article
Full-text available
The effect of selective antagonist of the arginine vasopressin (AVP) V1b receptors on the secretion of ACTH and corticosteroids in response to insulin-induced hypoglycemia and injection of AVP is studied in old Macaca mulatta females with depression-like and anxious behavior. Intravenous antagonist in a dose of 1.1-1.7 μg/kg inhibits the increase o...
Article
Full-text available
Aging is characterized by disturbances in the functioning of the hypothalamic-pituitary-adrenal (HPA) axis, associated with disturbances in the adaptation processes and increase of the probability of the onset of post-stress syndrome. However, the individual features of age-related disorders stress reactivity of HPA axis have not been studied. The...
Article
Full-text available
Individual features of the response of the hypothalamic-pituitary-adrenal axis (HPAA) to repeated moderate stress exposure (daily 2-h restraint stress for 10 days) was studied in young female rhesus monkeys with healthy normal behavior and combined group of female rhesus monkeys with abnormal depression-like and anxious behavior. No between-group d...
Article
Full-text available
Stress adaptation is fundamental for health, and the hypothalamic-pituitary-adrenal axis (HPA) is one of its main mechanisms. Considerable data indicate that arginine vasopressin (AVP) related disturbances of stress adaptation can occur with aging. However, most studies of such kind have been performed on rodents, give contradictory results and fai...
Article
Full-text available
Experimental study was carried out on young mature (6-8 years) and old (21-27 years) rhesus macaques with anxious and depression-like behavior and with standard (control) adaptive behavior. The responce of the adenohypophysis to arginine vasopressin depended on age and the type of adaptive behavior. Young animals with standard behavior demonstrated...
Article
Full-text available
The aim of the investigation was comparative study of the influence tetrapeptide Pancragen (St. Petersburg Institute of Bioregulation and Gerontology, St. Petersburg) on hormonal function of the pancreas compared to the effect of widely used hypoglycemic drug - glimepiride. The investigation involved 9 old (20-25 years) clinically healthy rhesus mo...
Article
Full-text available
A marked increase elderly population in the structure of modern society is one of the main reasons for increase in the number of patients with type 2 diabetes and impaired glucose tolerance. The purpose of this research was to study impact of pancragen (tetrapeptide Lys-Glu-Asp-Trp) on endocrine function of the pancreas of non-human primates, femal...
Chapter
The hypothalamic-pituitary-adrenal (HPA) axis plays a key role in adaptation to environmental stresses. Parvicellular neurons of the hypothalamic paraventricular nucleus secrete corticotrophin releasing hormone (CRH) and arginine vasopressin (AVP) into pituitary portal system; CRH and AVP stimulate ACTH release through specific G protein-coupled me...
Article
Full-text available
Abstract This review summarizes the results of the author’s works dedicated to age-related changes of the hypothalamic-pituitary-adrenal (HPA) axis in primates during aging under basal conditions, including disturbances of circadian rhythms, and under conditions of its inhibition and activation by specific stimuli. In addition, this article present...
Article
Full-text available
The article presents a review of the results of the author`s works that examine the character of age-related changes of the hypothalamic-pituitary-adrenal (HPA) axis in primates during aging in basal conditions, including disturbances of its circadian rhythms and in conditions of its inhibition and activation by specific stimuli. In addition, the o...
Article
Full-text available
We have investigated age-related changes in the reliability of glutathione-related antioxidant enzyme defense in monkeys that differ in adaptive behavior. Activities of gluthatione reductase (GR), glutathione peroxidase (GSH-Px), and gluthatione-S-transferase (GST) and also lipid peroxidation products (TBARS) under basal conditions and under acute...
Article
Full-text available
The hypothalamic–pituitary–adrenal (HPA) axis plays a key role in adaptation to environmental stresses. Parvicellular neurons of the hypothalamic paraventricular nucleus secrete corticotrophin releasing hormone (CRH) and arginine vasopressin (AVP) into pituitary portal system; CRH and AVP stimulate adrenocorticotropic hormone (ACTH) release through...
Article
Full-text available
Young (6-8 years) and old (21-30 years) Macaca mulatta females were subjected to gentle immobilization (2 h daily at 15.00) for 10 days. Blood specimens were collected before the exposure and 15, 30, 60, 120, 240 min and 24 h after the beginning of exposure on days 1, 3, and 10. The adrenocortical reaction to stress was maximum on day 1 in all anim...
Article
Full-text available
We have investigated age-related changes in the activity of antioxidant enzyme system and intensity of lipid peroxidation in erythrocytes of young and old female rhesus monkeys with depression-like and standard behavior. Revealed significant disturbances in the activities of GR and GSH-Px in monkeys with depression-like behavior are accompanied mor...
Article
Full-text available
We have investigated aging of the hypothalamic-pituitary-adrenal (HPA) axis in female rhesus monkeys that differ in adaptive behavior. Plasma cortisol (F) and dehydroepiandrosterone sulfate (DHEA-S) concentrations under basal conditions and under acute psycho-emotional stress were evaluated in blood plasma of young (6-8 years) and old (20-27 years)...
Chapter
Full-text available
The hypothalamic-pituitary-adrenal (HPA) axis is all-important adaptive neuroendocrine system with the circadian periodicity in the activity. There is some evidence to suggest that the circadian periodicity in functioning of the HPA deteriorates with aging and that these disturbances play an important role in age-related changes of glucocorticoid-s...
Article
Full-text available
The purpose was to study age-related changes of the hypothalamic-pituitary-adrenal (HPA) axis function and their interrelations with features of cognitive aging in individuals with various type of adaptive behaviour on model of laboratory primates. Plasma cortisol (F) and dehydroepiandrosterone sulfate (DHEAS) levels in basal conditions (0900h and...
Article
Full-text available
Clinical and experimental data point to existence of disturbances of adaptive ability of aged organism to extreme impacts. However mechanisms of these disturbances are not clear yet. The purpose of the investigation was to study age-related changes in reaction of erythrocyte antioxidant enzyme system in response to acute psycho-emotional stress and...
Article
The objective of this study was to evaluate the role of the adrenal cortex in the regulation of antioxidant enzyme defense and to characterize this regulation in different age periods. Five young and five old female rhesus monkeys were subjected to 2 hours squeeze cage restraint stress at 0900 or 1500 hours. Plasma levels of corticosteroids and act...
Article
Full-text available
The purpose of the investigation was to study age-related changes in reaction of the hypothalamic-pituitary-adrenal axis and erythrocyte antioxidant enzyme system in response to acute psychoemotional stress in non human primates. Ten young (6-8 years) and ten old (20-26 years) healthy female rhesus monkeys were subjected to acute moderate psycho-em...
Article
Full-text available
Activity of glutathione-dependent antioxidant enzymes (glutathione peroxidase, glutathione reductase, and glutathione transferase) in blood erythrocytes from female Macaca mulatta (6-8 and 20-26 years) was measured during activation of the hypothalamic-pituitary-adrenal system under conditions of acute psychoemotional stress (2-h restraint stress)....
Book
Full-text available
Goncharova N. D., Khavinson V. Kh., Lapin B. A. Pineal gland and age-related pathology (mechanisms and correction) – St. Petersburg: Nauka, 2007, 220 p. This monograph displays the results of an original study performed by the authors. This experimental study was aimed at the age-related pineal gland function alterations, as well as the role of age...
Article
Full-text available
In the course of aging both monkeys and people reveal decreased night and average daily level of melatonin in the blood plasma and reduced hormone circadian rhythm amplitude, which evidence the disorder of the pineal gland melatonin releasing function. Peptide preparations of the pineal gland (Epithalamin--a complex of peptides isolated from the pi...
Conference Paper
The goal of this research was to investigate if superoxide radical, which arises as the by-product of respiration, may be responsible for free-radical damages in cells and tissues of animals affected by psycho-emotional stresses. Female Macaca mulatta monkeys, 6-8 years (young) and 20-27 years (old), were subjected to the two-hours' immobilization...
Article
Young rhesus monkey females (Macaca mulatta) demonstrate the well-defined circadian rhythm in activity of erythrocyte SOD with maximum at 10.00 h and minimum at 22.00 h. However, neither GSH-Px nor GR demonstrated any significant circadian changes, contrastingly to SOD. The diurnal changes in the SOD activity tightly correlate with the diurnal chan...
Article
Increasingly more data appear that reactive oxygen species (ROS) like superoxide radical and its reactive chemical products induce not only destructive effects for organism but play important role in different physiological processes. On the other hand, the same is a true for the data certifying corticosteroids, for primates – cortisol (F), dehydro...
Article
Full-text available
Age-specific differences in the reaction of the hypothalamic-pituitary-adrenal system to acute psychoemotional stress (immobilization) was studied in female rhesus macaques aged 6-8 and 20-27 years at different time of the day. The reactivity of the hypothalamic-pituitary-adrenal system during immobilization at 15.00 was lower in old animals, while...
Article
The purpose of this research was to study age-related changes in functioning of pineal and pancreatic glands of non-human primates, rhesus monkeys, and to elucidate the possibility of their corrections with the help of epitalon, a synthetic analogue of the pharmacopoeia drug epithalamin. In old (20-27 years) animals, the basal plasma levels of gluc...
Article
Full-text available
The article presents a review of literature data on one of topical problems of contemporary endocrinology, developmental biology and gerontology--age-related changes of synthesis of adrenal androgens (dehydroepiandrosterone and dehydroepiandrosterone sulphate), and bioregulation of their production in man and monkeys.
Article
Full-text available
We studied the effect of Epithalon on the function of pancreatic islets and regulation of blood glucose level in female rhesus monkeys of various ages. Epithalon corrected the age-related decrease in glucose tolerance and restored the dynamics of insulin level in response to glucose load.
Article
Peculiarities of functioning of some parts of the endocrine system (the pineal gland, pancreatic gland, hypothalamic-pituitary-adrenal axis, and hypothalamic-pituitary-testicular axis) in an aging nonhuman primate model (Papio hamadryas and Macaca mulatta) are described in this article. It has been established that basal activity of some endocrine...
Article
Full-text available
Age-associated changes in the concentrations of glucose, insulin, and DHEAS in peripheral blood plasma of female rhesus macaques were studied in intact animals and in response to a standard dose of glucose and insulin. Basal levels of insulin and glucose increased, insulin sensitivity and glucose tolerance decreased, and pancreatic reaction to gluc...
Article
Full-text available
Investigation of the age-related changes of the pineal gland function and possible ways for their overcoming on nonhuman monkey model was the purpose of this study. Hormonal function of the pineal gland was studied in 38 Macaca mulatta females of two age groups: 6-8 years old, n = 18 and 20-26 years old, n = 20. Pineal function was studied in basal...
Article
Full-text available
This review discusses age-specific functional changes in different components of the endocrine system (pituitary, epiphysis, adrenals, and gonads), their role in aging and age-specific diseases, and possible approaches to correction of endocrine disorders and prevention of accelerated aging.
Conference Paper
NONHUMAN PRIMATES AS A MODEL FOR HUMAN ENDOCRINOLOGY AGING Nadezhda D. Goncharova, Boris A. Lapin (Institute of Medical Primatology, Russian Academy of Medical Sciences, Sochi-Adler, Russia) E-mail: iprim@ sochi.net Purpose: Humans and nonhuman primates exhibit striking kinship in reproductive physiology including both ovarian-menstrual cycles (whi...
Article
The study was aimed at characterizing the changes in hypothalamic-pituitary-adrenal (HPA) axis function during aging in monkey models (Papio hamadryas and Macaca mulatta). It has been established by specific radioimmunoassay and enzyme immunoassay that basal plasma levels of adrenal androgenes (dehydroepiandrosterone-DHEA, dehydroepiandrosterone su...
Article
Full-text available
Unlabelled: The OBJECTIVE of this research was to investigate the regulatory effect of Epitalon on the production of melatonin and cortisol in senescent monkeys. Material and methods: We investigated the character of melatonin and cortisol secretion by immunoferment assay in Epitalon-administered female Macaca mulatta in different age periods....
Article
Full-text available
The effect of Epithalon on melatonin and cortisol secretion in female rhesus monkeys of various ages was evaluated by enzyme immunoassay. Epithalon stimulated evening melatonin production and normalized circadian rhythms of cortisol production in old monkeys.
Article
Full-text available
Main results on studying properties of the new group of substances, Iuteinizing hormone-releasing hormone (LH-RH) agonists, on monkeys (Papio hamadryas) carried out in the Research Institute of Experimental Pathology and Therapy AMS of the USSR, Sukhumi, Research Institute of Medical Primatology RAMS, Sochi-Adler are presented in this article. Expe...
Article
Full-text available
Comparative studies on the functioning of the adrenal cortex in female rhesus macaques (Macaca mulatta) of different ages are reported - animals were aged 6-9 years (young adults; n = 5) and 20-26 years (old adults; n = 5). Corticosteroid concentrations (cortisol (F) and dehydroepiandrosterone sulfate (DHEAS)) were determined by specific radioimmun...
Article
The purpose of this study was to characterize the changes of hormonal function of the adrenals and gonads during aging in male baboons (Papio hamadryas). Basal levels of plasma dehydroepiandrosterone, dehydroepiandrosterone sulfate, pregnenolone, and 17-hydroxypregnenolone progressively decrease with age from 10-15 years when analyzed by specific r...
Article
Full-text available
Similar changes in the functioning of the adrenal glands (activation of cortisol synthesis due to a more complete utilization of its biochemical precursors and reduced formation of dehydroepiandrosterone and its sulfate fraction) are demonstrated in men and monkeys in hemoblastoses and during aging. It is assumed that being adaptive and nonspecific...
Article
Full-text available
Acute effects of agonists of luteinizing hormone releasing factor (LHRF) on the pituitary, testicular, and ovarian function in monkeys Goncharova N.D. Institute of Medical Primatology RAMS, Sochi The biological activities of LHRF superactive agonists surfagon (Russia), a promising agent for correcting many disorders of the reproductive function,...
Article
Full-text available
Работа посвящена сравнительному изучению гормональной функции надпочечных и половых желез у человека и обезьян в различные возрастные периоды. Обследовано 93 мужчины в возрасте от 22 до 90 лет и 37 самцов павианов гамадрилов от 6 до 26 лет. Уровень стероидных гормонов, их предшественников и метаболитов определяли в плазме периферической крови с пом...
Article
The work deals with a comparative study of hormonal function of adrenals and gonads in men and monkeys at different age periods. The total of 93 men aged from 22 to 90 years and 37 male baboons aged from 6 to 26 years were studied. The level of steroid hormones, their precursors and metabolites was determined in the peripheral blood plasma by a rad...
Article
Full-text available
The levels of bioactive LH, major sex and adrenal steroid hormones and their precursors in the system of biosynthesis were measured in the peripheral blood of 37 male Papio hamadryas of different age under baseline conditions and after injection of ACTH, LH releasing hormone, and human chorionic gonadotropin. The measurements were carried out in ad...
Article
The author presents the results of study of dehydroepiandrosterone sulfate (DEAS) regulation in the circulation of male monkeys (P. hamadryas) of different age groups young nature, 8-10, and old, 26 y.o.) by functional tests with chorionic gonadotropin, and a sensitive and specific direct radioimmunoassay of plasma DEAS level developed by the autho...
Article
Full-text available
Age-Related Differences in the Regulation of Dehydroepiandrosterone Sulfate Levels in Peripheral Blood Plasma of Monkeys N. D. Goncharova Key Words: dehydroepiandrosterone sulfate; regulation; age-related differences; monkeys Although dehydroepiandrosterone (DHA) and its sulfate (DHAS) are quantitatively the major corticosteroids present in the...
Article
Full-text available
Although dehydroepiandrosterone (DHA) and its sulfate (DHAS) are quantitatively the major corticosteroids present in the human circulation, their physiological significance and regulation are not yet fully understood. There is evidence in the literature suggesting that these compounds may play important roles in the regulation of lipid and car-bohy...
Article
Full-text available
1. The process of the restoration of the function of the hypophyseal-gonadal system in baboons subjected to the prolonged administration of an LHRF agonist was characterized by the development of three principal phases, a latent phase, a phase of hyperfunction of the gonads, and a phase of return to the initial level of functioning. 2. The duration...
Article
Full-text available
Time course of the major corticosteroid hormones and the precursors in the biosynthesis chain was studied, as was the type of corticosteroid complex formation with transcortin in the peripheral blood plasma in male papua hamadryas exposed to a 4-16 week infusions of LH releasing factor agonists (buserelin, Hoechst A. G., Germany, and surfagon, Card...
Article
Full-text available
The author suggests a direct radioimmunoassay of dehydroepiandrosterone sulfate (DEA-S) in 0.05-2 microliters of human blood plasma, making use of an antiserum prepared by rabbit immunization with DEA-S hemisuccinate conjugate with bovine serum albumin and [7-3H]-DEA. The method is highly sensitive (0.039 mumol/l), specific, reliable, and easily re...
Article
Full-text available
The paper is concerned with the results of a study of the effects of chronic (12 weeks) subcutaneous infusion of surfagon (D-Ala6, des-Gly10-ethylamide of LH-RH, a daily dose of 3 micrograms/kg of body mass) using osmotic mini-pumps on hormonal function of the hypophyseogonadal system, spermatogenesis and testicular histology in experiments on Papi...
Article
Full-text available
The paper is concerned with the results of investigation into the nature of regeneration of function of the hypophyseo-gonadal system in 15 mature male Papio hamadryas, subjected to infusion of LH-RH agonists (buserelin, Hoechst A. G. Germany) and surfagon (All-Union Cardiology Research Center, USSR) using osmotic mini-pumps (Alzo Corp., Palo Alto,...
Article
The authors have summed up the results of development of enzyme immunoassay (EIA) for the determination of testosterone (T) in human and animal blood plasma. The method is based upon the use of rabbit antiserum to testosterone-3-(O-carboxymethyl) oxim with bovine serum albumin as a binding system, a conjugate of T with horse-radish peroxidase as a...
Article
Full-text available
The authors presented the results of a study of hormonal function of the hypophyseal-gonadal system in Papio hamadryas males subjected to continuous infusion of buzereline (LH-RH agonist) at a daily dose of 3 micrograms/kg of body mass for 4-6 weeks with the help of subcutaneously implanted osmotic mini-pumps. A two-phasic response of the system to...
Article
Full-text available
The paper is concerned with the results of experiments on male Papio hamadryas aimed at the investigation of their hypophyseal and gonadal endocrine function by intravenous drip with the help of subcutaneously implanted osmotic mini-pumps of buzerelin, a superactive LH-RH analog. A 2-phase response of the hypophyseal-gonadal system was revealed. Th...
Article
Full-text available
The results of a study of the nature of adrenocortical function in 40 patients with tumorous and nontumorous diseases and in 22 monkeys (P. hamadryas) suffering from hemoblastosis with raised glucocorticoid activity of the adrenal glands were presented. A tendency to a decrease in the levels of pregnenolone, 17-hydroxypregnenolone and 17-hydroxypro...
Article
The nature of interaction of cortisol with corticosteroid-binding globulin was studied in the blood plasma of normal subjects and patients with leukemia and lymphoma. Sex-dependent differences in cortisol interaction with corticosteroid-binding globulin were found in hemoblastosis patients. Male patients showed a decrease in the binding capacity of...
Article
Full-text available
The nature of interaction of cortisol with corticosteroid-binding globulin was studied in the blood plasma of normal subjects and patients with leukemia and lymphoma. Sex-dependent differences in cortisol interaction with corticosteroid-binding globulin were found in hemoblastosis patients. Male patients showed a decrease in the binding capacity of...
Article
Full-text available
Adrenal and gonad steroid hormones were investigated in the peripheral blood plasma of human beings and hamadryas baboons (Papio hamadryas) with hemoblastosis. The similar hormonal dysfunction of adrenal glands and gonads was found in human beings and monkeys. The dehydroepiandrosterone level was found to decrease significantly, while the cortisol...
Article
Full-text available
The steroid hormone patterns were investigated in peripheral blood of healthy subjects and patients with chronic lympholeukemia, chronic myeloleukemia and acute leukemia. In all types of leukemia, the plasma levels of dehydroepiandrosterone were relatively lower, while testosterone concentration changes appeared to be sex-dependent: testosterone le...
Article
Full-text available
The cortisol metabolism was studied in patients with different variants of acute leukemia. In total amount of C21-steroid excreted with urine the portion of cortisol was decreased, the increased cortisol conversion into 20-dihydrometabolites and the decreased cortisol portion, conversed through the stage of cortisone into tetrahydrocortisone, were...

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