Nadezhda Golubkina

Federal Scientific Center of Vegetable Production · Laboratory Analytical Department

The relationships of selenium (Se) with other elements in plants is important for producing functional food with high Se contents and a predicted quality. To unveil the peculiarities of the element interactions, eight botanical varieties of Brassica oleracea L. were grown in similar conditions with or without foliar application of sodium selenate....

Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus are the primary bacteria that cause clinical infections, such as urinary and intestinal infections, pneumonia, endocarditis, and sepsis. Bacterial resistance is an innate natural occurrence in microorganisms, resulting from mutations or the lateral exchange of genetic material. Thi...

The evaluation of A. cepa biodiversity provides onion breeders with great prospects to obtain plants with high resistance to environmental factors, remarkable yield, and product quality. Genetic diversity assessment of a collection of nine short-and long-day onion landraces and varieties originated from different Iranian regions, using ISSR markers...

Biofortification of Brassica oleracea with selenium (Se) is highly valuable both for human Se status optimization and functional food production with direct anti-carcinogenic activity. To assess the effects of organic and inorganic Se supply for biofortifying Brassica representatives, foliar applications of sodium selenate and selenocystine (SeCys2...

The complex evaluation of varietal biochemical differences in rhubarb juice, pomace and roots is highly useful to develop an efficient processing technology. Research was carried out to compare four rhubarb cultivars (Malakhit, Krupnochereshkovy, Upryamets and Zaryanka) in terms of the quality and antioxidant parameters of juice, pomace and roots....

The ‘edge’ effect is considered one of the fundamental ecological phenomena essential for maintaining ecosystem integrity. The properties of plant outer tissues (root, tuber, bulb and fruit peel, tree and shrub bark, leaf and stem trichomes) mimic to a great extent the ‘edge’ effect properties of different ecosystems, which suggests the possibility...

Willow bark is highly valuable in medicine, different branches of industry and ecology. In this respect antioxidants and minerals distribution between bark tissues seems to be highly valuable. Using ICP-MS mineral composition of willow bark cambium, phloem and periderm was determined for the first time. Predominance of P, K and Ca was recorded for...

Selenium (Se) biofortification of aromatic plants is a promising strategy to produce valuable functional food with high biological activity and enhanced essential oil yield. The experiment carried out in 2021 and 2022 on A. annua treated with sodium selenate or nano-Se sprayed on foliar apparatus demonstrated a significant increase in photosyntheti...

Despite the high value of ramson (Allium ursinum) in medicine and nutrition, it is not cultivated in open fields due to the need for shading as well as weeding during the early crop stages. Research was carried out in an open field with the aim to improve A. ursinum growth, through its intercropping with Armoracia rusticana (horseradish). In the la...

The importance of using the barks of trees and shrubs as powerful natural antioxidants suggests the necessity to evaluate the effect of different environmental factors on bark extracts’ quality. The determination of total antioxidant activity (AOA) and polyphenol content (TP) in the bark of 58 tree and shrub species from 7 regions differing in mean...

Biochemical and mineral peculiarities of plants inhabiting desert and semi-desert areas may provide important information about the mechanism of their adaptability and reveal the prospects of their utilization. Rheum tataricum L., known for its high tolerance to drought, salinity, and nutritional deficiency, is the least studied species of wild rhu...

Ethnopharmacological knowledge Cabbage (Brassica oleracea var. capitata) is not only a widely known vegetable but also a valuable medicinal plant. Cabbage's pharmacological properties were described already in antiquity. Numerous reports, published in recent years, confirm cabbage's healing properties, leading to a deeper analysis of its applicatio...

Planting time has an important impact on yield and quality performances of crop systems and, therefore, it is crucial to identify its most effective matching to each growing area. Research was carried out in southern Italy, in 2016-17 and 2017-18, to determine the effects of planting time applied at 6 levels (from 30 October to 29 March at 30-day i...

The unique biological properties of A. annua have stimulated the research on its cultivation in different regions of the world. In this study, the effect of the Se and nano-Si supply on the yield, biochemical characteristics and mineral content of A. annua was investigated. Growth stimulation and a significant increase in the antioxidant status wer...

The need of sustainable management system is connected both to environmental safeguarding and to the increasing consumers’ demand for safety and premium-quality food. A research was carried out to assess the interactions between 2 farming systems (organic, conventional), 4 nitrogen doses (100, 133, 166.5, 200 kg•ha⁻¹ N) and 11 cultivars on yield, q...

Protection of plants against herbivorous pests is an important aspect that guarantees agricultural efficiency, i.e., food provision to populations. Environmental, water and foodstuff pollution by toxic pesticides, along with climate changes, highlight the necessity to achieve intensive development of ecologically safe methods of herbivory control....

Microalgae attract scientific interest due to their high potential as ecofriendly biostimulants, biofertilizers and bioprotectants against biotic and abiotic stresses. Literature reports reveal the positive effects of microalgae application to crop systems in improving plant nutrient uptake and tolerance to stress However, high variability of their...

Lamb’s lettuce (Valerianella locusta L. Laterr.) is a leafy green vegetable that is rich in various biological active compounds and is widely used in ready-to-eat salads. The cultivation conditions and growth stage could affect the secondary metabolism in plants and thereby modify their food value. In the present study, the effect of selenium (Se)...

Production of functional food with high levels of selenium (Se) and iodine (I) obtained via plant biofortification shows significant difficulties due to the complex interaction between the two elements. Taking into account the known beneficial effect of silicon (Si) on plant growth and development, single and joint foliar biofortification of chervi...

Relevance. Evaluation of nutritional value of seeds of agricultural crops is considered to be highly significant for revealing new sources of antioxidants for humans. Material. The aim of the present investigation was antioxidant status and selenium accumulation levels by chicory seeds (13 cultivars) and comparison of the results with antioxidants...

Background: Biofortification of vegetables with selenium (Se) greatly depends on species tolerance to Se supply. Due to the scant information regarding kohlrabi Se biofortification, the aim of the present work was the evaluation of foliar sodium selenate application on yield and biochemical characteristics of three kohlrabi cultivars. Material and...

The essentiality of selenium (Se) and iodine (I) to human beings and the widespread areas of selenium and iodine deficiency determine the high significance of functional food production with high levels of these elements. In this respect, joint biofortification of agricultural crops with Se and I is especially attractive. Nevertheless, in practice...

Relevance. High voltage electric power transmission lines (HVEPTL) cover a fairly large area of agricultural land all over the world. Investigations of electromagnetic field effect on growth and development of plants are held in various countries. The reaction of individual plant species and even varieties to the electromagnetic field manifests its...

Artemisia species and radish demonstrate intensive allelopathic properties and high ability to hyperaccumulate heavy metals, but their interaction has not been studied up to date. In a pot experiment, the relationship between wormwood (A. scoparia L.) and radish grown individually or intercropped, without and with Pb(NO3)2 supply, was investigated....

Intensive space exploration includes profound investigations on the effect of weightlessness and cosmic radiation on plant growth and development. Tomato seeds are often used in such experiments though up to date the results have given rather vague information about biochemical changes in mature plants grown from seeds subjected to spaceflight. The...

Introduction. Considering the essential biological role of selenium, including its role in preventing cardiovascular diseases and its widespread deficit in soils, assessing selenium status for Crimean region inhabitants is an actual task. Material and methods. Determination of selenium content in hair as index the of long-term element’s intake into...

The fragmentary literature data on Apiaceae seed antioxidant potential elicited a comparative evaluation work of seed biochemical profile between 11 species and 43 cultivars grown in similar conditions: anise, lovage, fennel, coriander, caraway, parsley, celery, dill, carrot, parsnip and chervil. Among the different solvents, temperature and durati...

The tomato industry has been searching for new genotypes with improved fruit production, both in the field and industrially processed, together with high-quality performance under sustainable management conditions. This research was carried out in Southern Italy with the aim of assessing the effects of industrial processing on the yield and quality...

Relevance. Pumpkin is one of the most important source of carotenoids for humans: β- and α-carotene, lutein and zeaxanthin playing a fundamental role in providing twilight and color vision accordingly. Results. Investigation of pumpkin carotenoid composition, Konfetka variety, revealed for the first time that this cultivar is the only one containin...

Relevance. Production of functional food with high levels of antioxidant status and selenium is essential for human protection against viral and cardiovascular diseases as well as cancer. Methods. Evaluation of the efficiency in foliar sodium selenate biofortification (25. 50 and 75 mg Se/L) was achieved on three chervil genotypes. Results. Interva...

Efficiency in detecting of tomato forms with no chalcone-naringenin flavonoid in pink-fruited and yellow-fruited forms was evaluated using DNA markers for various polymorphisms of the SlMYB12 gene. The closest relationship between a phenotype with the transparent skin of fruits and a deletion in the promoter region of the SlMYB12 gene was shown. Th...

Amaryllidaceae family comprises many crops of high market potential for the food and pharmaceutical industries. Nowadays, the utilization of plants as a source of bioactive compounds requires the plant/endophytic microbiome interactions, which affect all aspects of crop's quantity and quality. This review highlights the taxonomy, ecology, and bioac...

Relevance . A rare representative of the Apiaceae family the garden chervil is widely used abroad in nutrition and in folk and traditional medicine. Methods . Evaluation of nutritional potential of Anthriscus cerefolium (L.) Hoffm and Anthriscus sylvestris Hoffm grown in Moscow region is achieved using the parameters of ascorbic acid, polyphenols,...

Up-to-date genetic peculiarities of macro- and trace element accumulation in medicinal aromatic plants have been poorly investigated due to the predominant effect of geochemical characteristics of soils and the main attention paid to the features relevant to biologically active compounds and essential oil biosynthesis. Artemisia genus belongs to As...

Relevance. Biofortification of agricultural crops with selenium is considered to be the most promising method for the human selenium status optimization. Methods. Effect of foliar biofortification of cauliflower, Polyarnaya Zvezda cv, with sodium selenate of different concentrations on yield, selenium content and biochemical characteristics of plan...

The essentiality of selenium (Se) and iodine (I) for the human organism and the relationship between these two trace elements in mammal metabolism highlight the importance of the joint Se-I biofortification to vegetable crops in the frame of sustainable farming management. A research study was carried out in southern Italy to determine the effects...

Functional food with high concentrations of monosaccharides and antioxidants is important for quick replenishment of energy reserves and rehabilitation after intensive oxidant stress. The effect of high temperature and humidity for manufacturing such products from parsnip roots of three cultivars (Zemchug, Krugly, Bely aist) compared to garlic bulb...

Relevance. Medicinal properties of celery (Apium graveolens L.) are connected to the most extent to high antioxidant status of plants.Material, methods and results. Comparative evaluation of polyphenols, ascorbic acid, flavonoids and photosynthetic pygments by leafy, stalk and root celery types, grown in similar conditions on experimental fields of...

Celery is one of the major nutraceutical vegetable species due to the high dietary and medicinal properties of all of its plant parts. Yield, growth and produce quality of six celery genotypes belonging to leafy (Elixir and Samurai), stalk (Atlant and Primus) or root (Egor and Dobrynya) types, as well as the distribution of biomass, sugars, mineral...

The monograph presents the most simple and widely used methods for determining the most important of plant antioxidants: vitamin C, polyphenols, carotenoids, capsaicin, and belinovich photosynthetic pigments, flavonoids, anthocyanins, alkaloids, tannins, and minerals antioxidant: selenium and iodine. Special attention is paid to methods of extracti...

Relevance . Allium cepa L. is considered to be one of the most important agricultural crops in most countries of the world. High nutritional value is especially pronounced in salad onion which vegetation needs high temperature and significant insulation. Material and methods . The first biochemical characteristics are given for 9 Crimean salad onio...

Utilization of arbuscular mycorrhizal fungi (AMF) for enhancing growth and development as well as production of essential oil in aromatic plants has been increasingly drawing research interest. In order to assess the AMF effects on different aromatic species, an open-field experiment was carried out using Artemisia dracunculus (tarragon), Lavandula...

The need to improve crop yield and quality, decrease the level of mineral fertilizers and pesticides/herbicides supply, and increase plants' immunity are important topics of agriculture in the 21st century. In this respect, arbuscular mycorrhizal fungi (AMF) may be considered as a crucial tool in the development of a modern environmentally friendly...

Biofortification of garlic and onion plants with selenium and arbuscular mycorrhizal fungi inoculation are considered beneficial for producing functional food with anticarcinogenic properties. The effects of arbuscular mycorrhizal fungi (AMF) inoculation, sodium selenate foliar application, and the combination AMF + selenium (Se), compared to an un...

Celery and parsnip are highly valuable root vegetables and medical plants all over the world. Beneficial effect of plants on human health is connected not only with high content of biologically active compounds but also with specific levels of minerals reflecting geochemical characteristics of habitat, climate and genetic peculiarities. Using ICP-M...

Onion (Allium cepa L.) bulbs are commonly used as food or as a herb, showing pharmacological activities connected to natural beneficial compounds such as thiosulfinates, saponins, polyphenols, and flavonoids. The free amino acids arginine and glutamine are correlated with the taste intensity. Onion is a hyperaccumulator of selenium which reduces he...

The content of mobile forms of chemical elements in soils is affected by many factors including soil pH, grading and humus concentration. The most pronounced influence on elements behavior and their absorption levels by plants is produced by pH, which gives an opportunity to characterize quantitatively the ratio between elements forms in the soluti...

Research was carried out on onion landrace (Ramata di Montoro) for seed production in southern Italy, with the aim to evaluate the effects on yield and quality of four bulb planting times in factorial combination with four densities, using a split plot design with three replicates. The number of flower stalks per plant, their height and diameter, a...

High nutritional value of parsley determines constant interest to this agricultural crop as a source of natural antioxidants to humans. Biochemical characteristics of 6 parsley varieties (Federal scientific center selection) are investigated. Dry matter content was in the range 20.56-25.92%. Monosaccharides concentrations varied from 1.29 to 2.14%....

Chicory is widely cultivated in many countries of the world due to high nutritional and pharmacological value. The possibility of chicory forcing in winter provides quick production of salad heads, which biochemical characteristics are not fully described. Concentration of sugars, water soluble compounds and antioxidants in roots, leaves and stumps...

With the aim of assessing the effects of beneficial microorganisms on greenhouse tomato “plum” grown under salinity conditions, research was carried out in southern Italy from summer to winter, by comparing two arbuscular mycorrhizal fungi (AMF) based formulates (Rizotech Plus, Myco Apply DR) and a non-inoculated control, in factorial combination w...

Plant biofortification with selenium in interaction with the application of an arbuscular mycorrhizal fungi (AMF)-based formulate,with the goal of enhancing Se bioavailability, is beneficial for the development of the environmentally friendly production of functional food with a high content of this microelement. Research was carried out in order t...

The requirements for garlic powder enriched with selenium, dried by convective drying and homogenized, are established. Selenium-enriched vegetables have special healing properties and are of interest as a source of selenium. Garlic enriched with selenium is grown in a number of countries (including the USA, Sabinsa) and produced in the form of cap...

One of the urgent tasks at the present time is to obtain and widely use functional foods that have antioxidant and anticarcinogenic effects, which serve not only to meet human needs in proteins, fats, carbohydrates, micro - and macronutrients, but also contribute to the immune system, improve the heart and other human organs. For food plants, rich...

Sprouts of agricultural crops seeds are considered to be functional food products with high content of antioxidants. Though seeds of Allium species plants are used in some countries as spices the possibilities of appropriate sprouts utilization have never been investigated so far. Evaluation of perennial and A. cepa seeds biofortification with sele...