Nader Asadzadeh

• Doctor of Philosophy
• Deputy of Research, Technology & Findings dissemination, at Animal Science Research Institute

Supplementing freeze diluents with certain antioxidants can maintain the quality of chilled sperm. The present study was an attempt to investigate the effect of Beltsville extender supplementation with the mitochondrial‐targeted antioxidant ‘Mitoquinol’ on the quality parameters and fertility potential of rooster sperm during the cooling process. S...

Background: Sperm preservation at a cool temperature reduces sperm metabolism while preserving its viability and reproductive ability. Researchers have sought to extend semen preservation effectiveness for more than 24 hours. Due to the particular physiological characteristics of small ruminant spermatozoa, the cooling procedure decreases its repro...

Background Nutritional manipulation with functional nutrients like polyunsaturated fatty acids can boost milk production efficiency in dairy farming. It is important to consider the animal's physiological periods, especially the second half of the first pregnancy for mammary gland development. Objectives By considering multiple factors and compari...

Background: Although sperm cryopreservation seems to be an efficient technique for distributing competent sperm for artificial insemination, the process affects the quality of post-thawed sperm. Objectives: This study was designed to see how the novel mitochondria-targeted antioxidant “Mito-TEMPO” affected buck sperm quality during cryopreservation...

Cryopreservation of ram semen is helpful for distributing proved spermatozoa for reproductive goals, but cold shock has destructive effects on fertility ability of frozen sperm cells. This study was performed to investigate the effect of the novel mitochondria-targeted antioxidant "MitoQ" on ram sperm quality and fertility potential during cryopres...

Background Investigating genomic regions associated with morphometric traits in camels is valuable, because it allows a better understanding of adaptive and productive features to implement a sustainable management and a customised breeding program for dromedaries. Objectives With a genome‐wide association study (GWAS) including 96 Iranian dromeda...

Background The second half of the first pregnancy is a critical period in the growth and development of the mammary gland. The use of functional compounds during this period may positively impact livestock performance. Objectives In this study, changes in lipogenic enzyme gene expression in the mammary gland of Saanen goats in response to differen...

Sperm cryopreservation is used to spread qualified semen for artificial insemination, but the freezing process reduces sperm quality. This study assessed the efficacy of Mito-TEMPO on post-thawed goat sperm quality. Semen samples divided to five equal groups and after dilution, received different doses of Mito-TEMPO (0, 1, 10, 100 and 1000 μM), and...

Simple Summary The coat color of dromedary is usually uniform and varies from black to white. We identified 9 significant SNPs associated with white color, and the 13 significant SNPs associated with black color using genotyping-by-sequencing (GBS). Among candidate genes, SNAI1 that interacts with MCIR, ASIP and KIT genes plays a key role in the me...

Background: One of the significant challenges in camel husbandry is the lack of records and characterization of economically and health-relevant phenotypes. There are many fundamental problems due to their dispersion and the nature of the camel grassland farming specially phenotyping of camels. People's participation in the design and implementatio...

In the case of camels, there is little data in the literature on the relationship between body building features and the evaluation of milk yield. In the last decade, a rising interest in camel milk has been observed due to its nutritional and health-promoting properties, resulting in a growing market demand. Despite the remarkable importance of ca...

Growth is an important heritable economic trait for dromedaries and necessary for planning a successful breeding program. Until now, genome-wide association studies (GWAS) and QTL-mapping have identified significant single nucleotide polymorphisms (SNPs) associated with growth in domestic animals, but in dromedaries, the number of studies is very l...

Historically, female domestic goats carrying multiple kids are mostly unable to express sufficient nursing ability due to a limited number of functional teats. Therefore, the functional teat is an important component in prolific goat breeding, and plays a key role in the future health of their kids. With this motivation, we wanted to investigate th...

This study investigated the effects of supplementing Lake extender with cysteamine (CYS) on rooster semen quality in cold storage and it's fertility performance. Semen samples were diluted with Lake extender supplemented with different concentrations of CYS (0, 1, 2, 4 and 8 mM) and were cooled and stored at 5°C for a period of 46 h. Motility, memb...

The study aimed to compare the accuracy of seven Machine Learning methods for estimating the weight of dromedary camels, during birth-240 day of age, using the body measurements. With this mind, in overall, 458 records, including body weight and also 12 biometric linear measurements collected from dromedary camels at different stage of life, were u...

Sperm cryopreservation is used to spread qualified semen for artificial insemination, but the freezing process reduces sperm quality. This study assessed the efficacy of Mito-TEMPO on post-thawed goat sperm quality. Semen samples divided to five equal groups and after dilution, received different doses of Mito-TEMPO (0, 1, 10, 100 and 1000 μM), and...

Semen cryopreservation is an effective strategy for distributing spermatozoa for artificial insemination, but this process reduces the fertility potential of post-thawed spermatozoa. The current study was conducted to assess effects of the novel mitochondria-targeted antioxidant “Mito-TEMPO” on ram sperm quality and fertility potential during the f...

Abstract Rooster semen cryopreservation is a useful method to utilize semen samples for artificial insemination in commercial flocks, but with use of the freezing-thawing process there is a reduction in the quality and fertilization capacity of rooster spermatozoa post-thawing. The aim of the current study was to investigate the efficacy of the mit...

The PUFAs content of rooster sperm cells makes them vulnerable to the thermal shocks during chilling storage, which reduces the fertility performance of cooled sperm. Extender supplementation with antioxidants is a reasonable method to conserve sperm fertility potential during cooling storage process. The aim of this study was to determine the effe...

This study was aimed to evaluate the effect of addition of reduced glutathione (GSH) to the extender on the rooster's semen quality parameters and fertility potential. Semen samples were diluted with Lake extender contained 0, 0.5, 1, 2, 4 and 8 mM GSH. Then, were chilled to 5 °C and stored for a period of 48 h. Sperm motion characteristics, viabil...

Discovery of accurate, easy and economic embryo sex determination in the dairy cattle industry assumed highly particular importance. CffDNA is novel potential material to determine the sex of the fetus in early gestation period has not imposed negative effect on embryo health. In this regard, in overall, 16 Holstein cows experiencing eight weeks of...

Cell-free fetal DNA (cffDNA) can be detected in maternal blood during pregnancy, opening the possibility of early non-invasive prenatal diagnosis for a variety of genetic conditions as well as diagnosis of fetal sex. In this regard, in overall, 16 Holstein cows experiencing eight weeks of gestation and 4 non-pregnant Holstein cows, two bulls (negat...

Abstract In practice, worldwide horse breeders rely on genetic testing to generate pedigrees and protect studbook integrity. One of the most reliable and efficient method for pedigree construction and analysis is the DNA genotyping technology. Here we analyzed the usefulness of six microsatellites for routine parentage testing according to the pane...

Background and objectives: The beneficial effects of adding supplemental polyunsaturated fats including omega-6 and omega-3 sources to the diet on reproductive performance of livestock have been shown in many studies. On the other hand, low performance of artificial insemination is a serious problem to perform genetic improvement projects in sheep....

Abstract: The purpose of this study was to compare effects of adding soybean oil as omega-6 fatty acid source and palm hydrogenated oil as source of saturated fat to flushing diet on artificial insemination efficiency in Chaal ewes. One hundred eleven of non-lactating and non-pregnant Chaal ewes, were divided into three experimental groups based on...

Background and objectives: The beneficial effects of adding supplemental polyunsaturated fats including omega-6 and omega-3 sources to the diet on reproductive performance of livestock have been shown in many studies. On the other hand, low performance of artificial insemination is a serious problem to perform genetic improvement projects in sheep....

Abstract: It has been suggested that microsatellites contains many desirable properties, although they can be prone to genotyping errors which significantly affect the entire subsequent analysis. The purpose of the present study was to assess the comparison conventional and advance electrophoresis methods influencing the genotyping errors and stutt...

The broad goal of this research was to examine the nature of the Melanocortin receptor 1 (MC1R) locus on the coat color phenotype of black and white sheep. Blood samples were collected from 20 individuals and PCR-RFLP technique and DNA sequencing were used to detect polymorphism of the MC1R gene. Digestion of polymerase chain reaction products with...

Samples of grass pea seed (Lathyrus sativus) were prepared and analyzed for some chemical composition and as well for their anti-nutritional factors. The rumen degradability coefficients and post ruminal digestibility of dry matter and crude protein of unheated vs. oven heated samples were determined, employing in situ and in vitro techniques. Resu...

The aim of this study was to investigate the effect of urea treated baled wheat straw on fattening performance of male calves.The bales of wheat straw (about 10 kg each) were treated with urea solution to obtain 45% moisture and 4.5 % urea and were kept under plastic sheet for one month. Then the plastic was removed and samples of treated straw wer...

In order to investigation on possibility and accuracy of horse sex determination using PCR-RFLP polymorphism within ZFX/ZFY gene, whole blood samples of 20 Iranian arab horses (10 samples from each sex) were collected from Khouzestan province. DNA was extracted using modified salting-out procedure. Polymerase chain reaction (PCR) were done using pr...

Antibodies and their derivative fragments have long been used as tools in a variety of applications, in fundamental research work, biotechnology, diagnosis, and therapy. Camels produce single heavy-chain antibodies (VHH) in addition to usual antibodies. These minimal-sized binders are very robust and bind the antigen with high affinity in a monomer...

Vascular endothelial growth factor receptor-2 (VEGFR2) is an important tumor-associated receptor and blockade of the VEGF receptor signaling can lead to the inhibition of neovascularization and tumor metastasis. Nanobodies are the smallest intact antigen binding fragments derived from heavy chain-only antibodies occurring in camelids. Here, we desc...

Wool growth is influenced by photoperiodic period of different seasons; under similar nutritional conditions, more wool is produced in longer photoperiodic season. In this study, the effects of photoperiod on fiber and skin follicle characteristics of three sheep breeds were studied Forty non-pregnant and non-milking ewes (Chios, ArkhaMerino × Mogh...

In order to investigate the effects of cereal grain source in flushing ration onreproductive efficiency and serum concentrations of steroid hormones and metabolites, in reproductive season, 88 Shaal ewes were blocked by age then allocated into two groups on the basis of ration: 1- 300 gr corn grain plus basal diet (corn group); and 2- 300 gr barley...

Severe Combined Immllllodeficiency Disease (SCID) is an autosomal recessive hereditary disease occWTing among Arabian horses. The genetic defect responsible for this disease was recently identified as a 5-basepair deletion in the gene encoding DNA-Protein Kinase catalytic submrit (DNA-PKcs). Horses with one copy of the gene appear normal while hors...

In order to study the circadian rhythm of serum cortisol, metabolites, immunoglobulin and enzymes and blood cells in sheep; an experiment was conducted using 4 non-lactating and non-pregnant ewes (3.5-year-old). In summer solstice, from June 21 12:00 AM to June 22 12:00 AM (for 24 hours), blood samples were collected via catheterized jugular vein i...

In order to study physiological and metabolic factors related to ascites in broilers and effects of various types of bentonite on this factors, 200 Arian male chicks were experimented in a completely random design. At the age of 7, broilers were assigned to five equal groups based on diet: 1- diet without any forms of bentonite (control); 2- diet c...

The objective of this present study was to investigate relationships between the growth hormone gene restriction fragment length polymorphism (RFLP) and bull sperm characteristics. A total of 89 bulls from two semen evaluation stations were genotyped for the bovine growth hormone (bGH)-AluI polymorphism by polymerase chain reaction and followed by...

Variations at DNA level contribute to the genetic characterization of livestock populations and this may help to identify possible hybridization events as well as past evolutionary trends. The leptin and Pit-1 are attractive candidate genes for production and reproduction traits in cattle. A total of 247 animals from four breeds from two species of...

In order to investigate the effect of artificial photoperiod on reproductive performance in seasonally anestrous ewes, 40 non-pregnant Chios ewes in spring were allocated into two groups on the basis of photoperiodic patterns: 1- natural long day photoperiod group (LD) 2- artificial short day photoperiod group (SD). On April 12, ewes In SD group we...

Background: Scorpion envenomation is considered as one of the Public Health problems in some countries in the world including Iran. Annually, approximately 30,000 scorpion stings happen in Iran from which 12% belongs to Hemiscorpius lepturus (special small closely spaced, bead-shaped jointed tail, similar in the shape to a cows tail, and is locally...

The objective of this study was to assess the association of polymorphism in leptin gene with breeding value of milk traits in Iranian Holstein cattle. Traits analyzed were breeding values of Milk and Fat production. A strategy employing polymerase chain reaction was used to amplify a 422 bp from blood, semen. Digestion of polymerase chain reaction...

Problem statement: Variations at DNA level contribute to the genetic characterization of livestock populations and this may help to identify possible hybridization events as well as past evolutionary trends. Approach: The leptin and Pit-1 are attractive candidate genes for production and reproduction traits in cattle. A total of 247 Animals from fo...

In order to investigation of possibility and accuracy of horse sex determination using PCR-RFLP within ZFX/ZFY Genes, Whole blood samples of 20 Arab horses (10 samples from female and 10 samples from male) were collected from Khozestan province. DNA was extracted using the modified salting-out procedure. Polymerase chain reaction (PCR), for amplifi...

Objective of this study was comparing feedlot performance of dromedary and Bactrian×dromedary hybrid camels after a 5-month fattening duration. 14 Iranian Kalkoohi dromedary and 14 Bactrian×dromedary camels (12-month-old; 7 male and 7 female in each group) were used in a 2×2 factorial experiment. Main effects were level of genotype (dromedary or hy...

This study was conducted to compare feedlot performance of dromedary and Bactrian×dromedary hybrid camels after a 8-month fattening duration. 8 Iranian Kalkoohi dromedary and 8 Bactrian×dromedary camels (12-month-old; 4 male and 4 female in each group) were used in a 2×2 factorial experiment. Main effects were level of genotype (dromedary or hybrid...

To determine the effects of feedlot duration and sex on carcass characteristics, 12 Kalkoohi dromedary camels (12-month-old; 6 male and 6 female) were used in a 2×2 factorial experiment. Main effects were levels of feedlot duration (5 or 8 months) and sex (male or female). Camels in individual pens were fed diet containing 25% alfalfa, 25% wheat st...

Objective of this study was comparing feedlot performance of dromedary and Bactrian×dromedary hybrid camels after a 5-month fattening duration. 14 Iranian Kalkoohi dromedary and 14 Bactrian×dromedary camels (12-month-old; 7 male and 7 female in each group) were used in a 2×2 factorial experiment. Main effects were level of genotype (dromedary or hy...

The main goal of breeders is breeding superior animals. Most traits of the economic importance in farm animals are determined by polygenic loci with environmental factors. Progress in animal breeding may be improved by combining traditional performance data with molecular genetic information on quantitative loci in selection index. Candidate genes...

به منظور بررسي اثرات افزودن پيه گاو به جيره بر عملكرد پروار و قابليت هضم ظاهري مواد مغذي، 90 راس گوساله كردي با وزن اوليه 18±224 كيلوگرم با جيره‌هاي غذايي 1- بدون چربي مكمل (جيره شاهد) و 2- حاوي 5% پيه، به مدت 98 روز مورد آزمايش قرار گرفتند. در پایان دوره پروار از هر گروه 6 گوساله کشتار و خصوصیات لاشه ارزیابی شد. نمونه‌هاي خوراك و مدفوع آناليز شيمي...

In order to investigation of possibility and accuracy of horse sex determination using PCR-RFLP within ZFX/ZFY Genes, Whole blood samples of 20 Arab horses (10 samples from female and 10 samples from male) were collected from Khozestan province. DNA was extracted using the modified salting-out procedure. Polymerase chain reaction (PCR), for amplifi...

The use of polymorphic markers in breeding pro-grammes could make selection more accurate and efficient. A total of 324 individuals from six Iranian cat-tle populations (Sarabi, Golpayegani, Sistani, Taleshi, Mazandarani, Dashtiyari), F 1 Golpayegani × Brown Swiss and Iranian buffalo populations were genotyped for the Pit-1 HinfI and leptin Sau3AI...

‌Abstract Bovine leukocyte adhesion deficiency is a recessive autosomal disease of young Holstein cattle. This disease is caused due to change adenine in to guanine in CD18gene which result in replacement of an amino acid, aspartic acid into glycine at D128G position. CD18 is a surface glycoprotein of neutrophils which is capable of causing loss of...

An assessment was made of the risks of bovine spongiform encephalopathy (BSE) occurring in Argentina. Most of the factors associated with the origin and development of the BSE epidemic in the UK are essentially absent. For example, Argentina's large sheep and cattle industries are based on low-cost production systems using grass. Concentrated feeds...

Bovine spongiform encephalopathy (BSE) is a new member of the scrapie-family of diseases that was first recognized in the United Kingdom in November 1986 (1) and subsequently developed into a major epidemic. BSE was caused by the contamination of cattle feed with a scrapie-like agent (2). The vehicle of infection was meat and bone meal (MBM), which...

Bovine spongiform encephalopathy (BSE) is a new disease of cattle first described in the United Kingdom in November 1986. BSE belongs to the scrapie-related group of diseases. The epidemiological studies performed in the United Kingdom demonstrate that the BSE epidemic was caused by feeding cattle with ruminant-derived protein contaminated by a scr...