Nada Krasevec

Nada Krasevec
National Institute of Chemistry · Department of Molecular Biology and Nanobiotechnology

PhD

About

49
Publications
18,852
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1,329
Citations
Citations since 2016
13 Research Items
1022 Citations
2016201720182019202020212022050100150200
2016201720182019202020212022050100150200
2016201720182019202020212022050100150200
2016201720182019202020212022050100150200
Introduction
Recent and past scientific interests: 1. Fungal bioinformatics and –omics; 2. Function of fungal structural proteins; 3. Function of fungal enzymes; 4. Interaction of fungi with plants; 5. Recombinant protein production in fungi; 6. Gender dimensions in fungal research.

Publications

Publications (49)
Article
Full-text available
Aegerolysins are remarkable proteins. They are distributed over the tree of life, being relatively widespread in bacteria and fungi, but also present in some insects, plants, protozoa, and viruses. Despite their abundance in cells of certain developmental stages and their presence in secretomes, only a few aegerolysins have been studied in detail....
Article
Full-text available
The main drivers of gender mainstreaming in basic and clinical research appear to be funding agencies and scientific journals. Some funding agencies have already recognized the importance of their actions for the global development of ideas in science, but further targeted efforts are needed. The challenges for women scientists in fungal research a...
Article
Full-text available
This review discusses the inclusion of sex and gender variables in studies of fungal infections in humans at the pathogen, host, and antifungal trial levels. The mating type of some fungi, or perhaps more likely the absence of the other, appears to be associated with some infections. Sexual and parasexual reproduction of some fungi is an important...
Article
Full-text available
Fungi are the most common pathogens of insects and thus important regulators of their populations. Lipid-binding aegerolysin proteins, which are commonly found in the fungal kingdom, may be involved in several biologically relevant processes including attack and defense against other organisms. Aegerolysins act alone or together with membrane-attac...
Article
Full-text available
Aegerolysins are small lipid-binding proteins particularly abundant in fungi. Aegerolysins from oyster mushrooms interact with an insect-specific membrane lipid and, together with MACPF proteins produced by the same organism, form pesticidal pore-forming complexes. The specific interaction with the same membrane lipid was recently demonstrated for...
Article
Full-text available
Fungi have the ability to transform organic materials into a rich and diverse set of useful products and provide distinct opportunities for tackling the urgent challenges before all humans. Fungal biotechnology can advance the transition from our petroleum‑based economy into a bio‑based circular economy and has the ability to sustainably produce re...
Article
Full-text available
Proteins of the aegerolysin family have a high abundance in Fungi. Due to their specific binding to membrane lipids, and their membrane-permeabilization potential in concert with protein partner(s) belonging to a membrane-attack-complex/perforin (MACPF) superfamily, they were proposed as useful tools in different biotechnological and biomedical app...
Article
Full-text available
Proteins of the aegerolysin family span many kingdoms of life. They are relatively widely distributed in bacteria and fungi, but also appear in plants, protozoa and insects. Despite being produced in abundance in cells at specific developmental stages and present in secretomes, only a few aegerolysins have been studied in detail. In particular, the...
Article
Full-text available
Background The fungal genus Aspergillus is of critical importance to humankind. Species include those with industrial applications, important pathogens of humans, animals and crops, a source of potent carcinogenic contaminants of food, and an important genetic model. The genome sequences of eight aspergilli have already been explored to investigate...
Article
Hydroxysteroid dehydrogenase (17β-HSDcl) from the filamentous fungus Curvularia lunata (teleomorph Cochliobolus lunatus) catalyzes NADP(H)-dependent oxidoreductions of androgens and estrogens. Despite detailed biochemical and structural characterization of 17β-HSDcl, its physiological function remains unknown. On the basis of amino acid sequence al...
Article
Physical and functional interactions between molecules in living systems are central to all biological processes. Identification of protein complexes therefore is becoming increasingly important to gain a molecular understanding of cells and organisms. Several powerful methodologies and techniques have been developed to study molecular interactions...
Chapter
Due to their capacity for producing large amounts of secreted proteins, filamentous fungi have a long history of industrial use for the production of endogenous enzymes. While the genetic engineering tools developed for filamentous fungi expanded the use of fungi for the production of heterologous proteins, the production of recombinant proteins is...
Article
Full-text available
Bark beetles and their fungal associates are integral parts of forest ecosystems, the European spruce bark beetle (Ips typographus Linnaeus, 1758) and the associated pathogenic blue stain fungus Ceratocystis polonica (SIEM.) C. MOREAU, are the most devastating pests regarding Norway spruce [Picea abies (L.) H. KARST.]. Bark beetles commonly inhabit...
Article
Previously, we identified CYP53 as a fungal-specific target of natural phenolic antifungal compounds and discovered several inhibitors with antifungal properties. In this study, we performed similarity-based virtual screening and synthesis to obtain benzoic acid-derived compounds and assessed their antifungal activity against Cochliobolus lunatus,...
Article
Previously, we identified CYP53 as a fungal-specific target of natural phenolic antifungal compounds and discovered several inhibitors with antifungal properties. In this study, we performed similarity-based virtual screening and synthesis to obtain benzoic acid-derived compounds and assessed their antifungal activity against Cochliobolus lunatus,...
Article
Full-text available
The aegerolysin protein family (from aegerolysin of the mushroom Agrocybe aegerita) comprises proteins of ∼15-20 kDa from various eukaryotic and bacterial taxa. Aegerolysins are inconsistently distributed among fungal species, and variable numbers of homologs have been reported for species within the same genus. As such noncore proteins, without a...
Article
For the first time, a fungal production system is described for expression and secretion of the medically important human protein G-CSF, in Aspergillus niger. A reliable strategy was chosen with in-frame fusion of G-CSF behind a KEX2 cleavage site downstream of the coding region of the highly secreted homologous glucoamylase. This provided secretio...
Article
Full-text available
Cytochrome P450 monooxygenases (CYPs/P450s) are heme-thiolate proteins whose role as a drug target against pathogenic microbes has been explored because of their stereo- and regio-specific oxidation activity. We aimed to assess the CYP53 family's role as a common alternative drug target against animal (including human) and plant pathogenic fungi an...
Data
Supplemental Fig. 1 Diurnal course of sap flow in Norway spruce trees infected with the fungus Ceratocystis polonica (trees no. 1 and 2) and controls (trees no. 3 and 4) on representative days: (a) 18.4.2011, (b) 24.4.2011, (c) 16.5.2011, (d) 15.6.2011, (e) 10.7.2011,
Data
Supplemental Fig. 2 Changes in concentrations of defence compounds [(a) catechin, (b) astringin, (c) isorhapontin, (d) taxifolin, (e) total phenolics, (f) total glutathione, (g) glutathione reductase, (h) total cysteine, (i) total ascorbate] in Ceratocystis polonica infected trees when compared to the control trees. *Significantly different from th...
Article
Full-text available
Ethanolic extracts of mycelia from Aspergillus niger (strain N402) grown in liquid media were observed to have haemolytic activity on bovine erythrocytes. This haemolytic activity decreased significantly during the time of growth (1-3 days). Moreover, when A. niger was grown on carbon-deprived medium, the efficiency of this haemolytic activity in t...
Article
Cochliobolus is a young genus, in the class Dothideomycetes, which includes closely related plant pathogenic and saprophytic fungal species. In this review, genome similarities and differences among sequenced Cochliobolus pathogens that cause different diseases on different hosts are detailed. Gene content and genome organization are highly similar...
Article
Membrane Attack Complex/Perforin (MACPF) and Cholesterol-Dependent Cytolysins (CDC) form the MACPF/CDC superfamily of important effector proteins widespread in nature. MACPFs and CDCs were discovered separately with no sequence similarity at that stage being apparent between the two protein families such that they were not, until recently, consider...
Article
Full-text available
Key message Based on time courses of individual antioxidant compounds, bark phenolic metabolism has been recognised to integrate ascorbate–glutathione system as a redox hub in Norway spruce defence against Ceratocystis polonica infection. Abstract Temporal courses of individual phenolics, thiols and ascorbate were studied in Norway spruce phloem ov...
Article
Full-text available
Cytochrome P450 monooxygenases (P450s) are ubiquitous heme-thiolate proteins that have potential biotechnological application. Thermostable-P450s that can withstand hostile industrial conditions, such as high temperatures, extremes of pH and organic solvents, are needed for biotechnological usage. Here, for the first time, we report a large number...
Article
Full-text available
Among the most devastating pests of Norway spruce (Picea abies) are the European spruce bark beetle (Ips typographus) and the associated pathogenic blue-stain fungus Ceratocystis polonica. Following attack and colonization, the beetle and the fungus must cope with induced host chemical defenses, such as monoterpenes that are generally thought to be...
Article
CYP53A15, from the sorghum pathogen Cochliobolus lunatus is involved in detoxification of benzoate, a key intermediate in aromatic compound metabolism in fungi. Because this enzyme is unique to fungi, it is a promising drug target in fungal pathogens of other eukaryotes. In our work we showed high antifungal activity of seven cinnamic acid derivati...
Article
Aims: CYP53A15, from the sorghum pathogen Cochliobolus lunatus, is involved in detoxification of benzoate, a key intermediate in aromatic compound metabolism in fungi. Because this enzyme is unique to fungi, it is a promising drug target in fungal pathogens of other eukaryotes. Methods and Results: In our work, we showed high antifungal activity of...
Article
Fungal CYP53 enzymes are highly conserved proteins, involved in phenolic detoxification and have no homologues in higher eukaryotes, rendering them favorable drug targets. Aiming to discover novel CYP53 inhibitors, we employed two parallel virtual screening protocols and evaluated highest scoring hit compounds by analyzing the spectral binding inte...
Article
Cytochromes P450 (CYPs) catalyse diverse reactions and are key enzymes in fungal primary and secondary metabolism, and xenobiotic detoxification. CYP enzymatic properties and substrate specificity determine the reaction outcome. However, CYP-mediated reactions may also be influenced by their redox partners. Filamentous fungi with numerous CYPs ofte...
Article
A large number of proteins involved in calcium and intracellular pH signaling and homeostasis have previously been discovered and characterized in Saccharomyces cerevisiae, Homo sapiens and Arabidopsis thaliana,but relatively few have been identified in Aspergillus species. The aim of this study was to identify proteins regulating the intracellular...
Article
The identification and annotation of protein-coding genes is one of the primary goals of whole-genome sequencing projects, and the accuracy of predicting the primary protein products of gene expression is vital to the interpretation of the available data and the design of downstream functional applications. Nevertheless, the comprehensive annotatio...
Article
The cytochromes P450 (CYPs) are found in all biological kingdoms and genome sequencing projects continue to reveal an ever increasing number. The principle aim of this paper is to identify the complete CYPome of Aspergillus nidulans from the genome sequence version AN.3 deposited at the Broad institute, assign the appropriate CYP nomenclature and d...
Article
A novel cytochrome P450, CYP53A15, was identified in the pathogenic filamentous ascomycete Cochliobolus lunatus. The protein, classified into the CYP53 family, was capable of para hydroxylation of benzoate. Benzoate is a key intermediate in the metabolism of aromatic compounds in fungi and yet basically toxic to the organism. To guide functional an...
Article
Full-text available
Cytochrome P450 reductase (CPR) is the redox partner of P450 monooxygenases, involved in primary and secondary metabolism of eukaryotes. Two novel CPR genes, sharing 34% amino acid identity, were found in the filamentous ascomycete Cochliobolus lunatus. Fungal genomes were searched for putative CPR enzymes. Phylogenetic analysis suggests that multi...
Article
Full-text available
Suppression subtractive hybridization (SSH) was employed to study differential gene expression upon progesterone treatment of the filamentous fungus Cochliobolus lunatus, a plant and opportunistic human pathogen. The transcription profile of progesterone-induced vs. non-induced C. lunatus revealed changes in the number of genes involved in facilita...
Article
Full-text available
We studied secretion of human cytokine tumour necrosis factor alpha (TNF alpha) by the filamentous fungus Aspergillus niger. When the gene for glucoamylase was fused with TNF alpha, optimized for expression in E. coli (TNFE), TNF alpha was detected only inside cells, in the form of an uncleaved fusion protein. Non-optimized codon usage of TNFE prov...
Article
Full-text available
The most common expression strategy for secreting heterologous proteins from filamentous fungus Aspergillus niger is based on fusion with glucoamylase gene which contains cleavage site for kexin protease (KEX2). However, secretion of recombinant proteins in the form of a fusion-protein without a host-specific cleavage site is usually higher than se...
Article
A gene-fusion expression strategy was applied for the heterologous expression of hTNF-α in A. niger AB1.13. The TNF-α gene was fused with the A. niger glucoamylase GII form as a carrier-gene, behind its transcription control and secretion signal. The protein was expressed in the cells in the form of a glucoamylase-fusion protein, but was not presen...
Article
The cDNA encoding for catalytic subunit of bovine enterokinase (EK(L)), to which the sequence for Kex2 protease cleavage site was inserted, was expressed in the protease deficient filamentous fungus Aspergillus niger AB1.13. Fungal transformants were obtained in which expression of the glucoamylase fusion gene resulted in secretion of the protein i...
Article
Full-text available
A gene-fusion expression strategy was applied for the heterologous expression of hTNF-alpha in A. niger AB1.13. The TNF-alpha gene was fused with the A. niger glucoamylase GII form as a carrier-gene, behind its transcription control and secretion signal. The protein was expressed in the cells in the form of a glucoamylase-fusion protein, but was no...
Article
Full-text available
A gene-fusion expression strategy was applied for heterologous expression of human lymphotoxin alpha (LT&#102) in the Aspergillus niger AB1.13 protease-deficient strain. The LTf gene was fused with the A. niger glucoamylase GII-form as a carrier-gene, behind its transcription control and secretion signals. Special attention was paid to the influenc...
Article
Full-text available
Production of human tumor necrosis factor alpha (hTNF-alpha) in the expression system E. coli/pMAX resulted in high expression of the 25 kDa fusion protein appearing as an insoluble faction in inclusion bodies. Several isolation procedures, cleavage methods and renaturation procedures are described, which have been investigated in order to obtain a...

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