Na Tian

Na Tian
Boston University Henry M. Goldman School of Dental Medicine · Molecular and Cell Biology

DDS, MS, PhD

About

16
Publications
1,695
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
220
Citations

Publications

Publications (16)
Article
Full-text available
Celiac disease (CD) is a chronic immune-mediated enteropathy induced by dietary gluten in genetically predisposed individuals. Saliva harbors the second highest bacterial load of the gastrointestinal (GI) tract after the colon. We hypothesized that enzymes produced by oral bacteria may be involved in gluten processing in the intestine and susceptib...
Article
Gluten are proline- and glutamine-rich proteins present in wheat, barley and rye, and contain the immunogenic sequences that drive celiac disease (CD). Rothia mucilaginosa, an oral microbial colonizer, can cleave these gluten epitopes. The aim was to isolate and identify the enzymes and evaluate their potential as novel enzyme therapeutics for CD....
Article
Full-text available
Background: Celiac disease (CD) is an inflammatory disorder triggered by ingested gluten, causing immune-mediated damage to the small-intestinal mucosa. Gluten proteins are strikingly similar in amino acid composition and sequence to proline-rich proteins (PRPs) in human saliva. Based on this feature and their shared destination in the gastro-inte...
Article
Full-text available
Immunogenic gluten proteins implicated in celiac disease (CD) largely resist degradation by human digestive enzymes. Here we pursued the isolation of gluten-degrading organisms from human feces, aiming at bacteria that would digest gluten under acidic conditions, as prevails in the stomach. Bacteria with gluten-degrading activities were isolated us...
Article
Gluten proteins, the culprits in celiac disease (CD), show striking similarities in primary structure with human salivary proline-rich proteins (PRPs). Both are enriched in proline and glutamine residues that often occur consecutively in their sequences. We investigated potential differences in the spectrum of salivary proline-rich proteins in heal...
Article
Full-text available
Rothia mucilaginosa, a natural microbial inhabitant of the oral cavity, cleaves gluten (gliadin) proteins at regions that are resistant to degradation by mammalian enzymes. The aim of this study was to investigate to what extent the R. mucilaginosa cell-associated enzymes abolish gliadin immunogenic properties. Degradation of total gliadins and hig...
Article
During a survey of human saliva by a top-down reversed-phase high-performance liquid chromatography-electrospray ionization-mass spectrometry approach, two proteins eluting at 27.4 and 28.4 min, with average masses of 15 494 ±1 and 11 142 ±1 Da, were detected in a subject from Boston. The Δmass value (4352 Da) of the two proteins was similar to the...
Conference Paper
Objective: Human saliva is a mixed oral fluid containing a wide variety of bacteria. Saliva is constantly swallowed, reaching the more downstream regions of the gastrointestinal tract. Functions of saliva are therefore unlikely to be restricted to the oral cavity. We recently discovered that certain natural oral bacteria can cleave gluten proteins,...
Conference Paper
Objectives: Gluten proteins are implicated in celiac disease, which is characterized by duodenal inflammation. A life-long gluten-free diet is currently the only therapy available to celiac patients. Research in our laboratory has discovered that the oral cavity is a rich source of microorganisms capable of cleaving dietary gluten. The goal of our...
Conference Paper
Introduction: Celiac disease is a T cell mediated-inflammatory enteropathy caused by dietary gluten. Certain immunogenic domains in gluten are completely resistant to the major human digestive enzymes. Deamidation of gluten by transglutaminase-2 in the lamina propria greatly enhances their binding to antigen presenting cells, provoking an inflammat...
Article
Full-text available
There is growing interest in the use of human whole saliva for diagnostics and disease monitoring as an alternative to blood samples. In contrast to blood, whole saliva is a non-sterile body fluid. Proper hand-ling and storage are required to preserve the integrity of potential biomarkers. We investigated salivary autoproteolytic degradation using...
Conference Paper
Objectives: Periodontitis is an inflammatory disease initiated by bacteria and characterized in part by neutrophil-mediated tissue injury. P. gingivalis primes and preactivates neutrophils to secondary stimuli leading to tissue destruction. Resolvin E1 (RvE1) is a potent pro-resolving mediator of inflammation derived from omega-3 eicosapentaenoic a...
Article
Certain virulence factors participating in periodontitis may relate to cardiovascular diseases. This study was to evaluate the pro-apoptotic effect of protein extracts from Porphyromonas gingivalis on bovine aortic endothelial cells (BAECs). The BAECs were exposed to trypsin-like protease-active protein extracts from P. gingivalis, and apoptosis wa...
Article
To evaluate the effect of extracellular protein from Porphyromonas gingivalis on the viability of bovine aortic endothelial cells (BAECs). Extracellular protein was extracted from the culture supernatant of P. gingivalis ATCC 33277 by salt induced precipitation. In the experimental group, BAECs were treated by extracellular protein in different con...

Network

Cited By