Myroslav Parii

The present study reports an efficient in vitro plant regeneration system for amphidiploid (2n = 42) spelt wheat (Triticum spelta L.). Plant regeneration from mature embryos of winter spelt variety “Europe” was carried out as a two-step process. The first step was callus induction on a medium supplemented with 2 mg L−1 dichlorophenoxyacetic acid an...

The ability to express foreign genes in plant cells provides a powerful tool for studying the function of specific genes. In addition, the creation of genetically modified plants may provide new important features that are useful for industrial production or pharmaceutical applications. One of the key parameters for the development of a high level...

Aim. Genetic constructs creation for studying the influence effect of the viral posttranscriptional silencing protein suppressor p19 on transient reporter green fluorescent protein (GFP) expression and accumulation. Methods. The Golden Gate molecular cloning method was used to create the genetic constructs; the leafy tissues of the Aztec tobacco pl...

Aim. To create a genetic construct carrying the bacterial protein Cas9 gene, the reporter β-glucuronidase gus gene, as well as the marker phosphinotricin-N-acetyltransferase bar gene for plant genome editing. Methods. Molecular-biological, biotechnological, microbiological and bioinformatic methods were used in the study; Golden Gate molecular clon...

Aim. Acceleration of the sunflower lines homozygosity for isolation of the fertility restorer resistant to tribenuron methyl herbicide using in vitro culture of sunflower immature embryos. Methods. Methods of immature sunflower embryos cultivation in in vitro culture; methods of classical crossing. Results. As a result, homozygous genotype was crea...

One of the three genes of the endosperm structure is used in the selection of sweet corn; its recessive mutation sh2 activates the accumulation of sucrose in the structural complex of sugars and represses the starch synthesis. Hybrids of "super-sweet" corn have improved grain taste and slower conversion of sugars into starch. Therefore, it is impor...

Aim. To establish the optimal concentration of glyphosate for efficient selection of transgenic tissue culture in biotechnology of rapeseed by in vitro selection of glyphosate-resistant and non-resistant winter rape. Methods. As an explants 7–10 mm fragments of 6-day rapeseed hypocotyls were used to cultivate on the medium, supplemented with 1 mg/L...

Aim. To develop an effective protocol for callus induction from shoot apical meristem in Triticum spelta L. and T. aestivum L. Methods. Plant material: spelt “Europe” and common wheat “Bunchuk”. For this research we used shoot apical meristems from 3-days plants. For callus induction we proposed 4 media with different concentration of 2,4-D, piclor...

Aim. Creation of genetic constructions to study the effects of various regulatory elements, namely promoters, on the expression of GFP reporter protein. Methods. For creation genetic constructs, the method of molecular cloning Golden Gate was used, which allows the rapid creation of genetic vectors using IIS type restriction enzymes and T4 DNA liga...

Precise chromosome segregation is vital for speciation and hybrid formation. The aim of this work was to study the chromosomes behavior and inheritance of maternal and paternal genomes in Arabidopsis regenerants obtained from in vitro cultured cells on the medium with para-fluorophenyalanine (PFPA). The Arabidopsis thaliana model hybrid between Col...

The analysis of Ukrainian isolate of beet necrotic yellow vein virus has been performed. The partial nucleotide sequence of cDNA corresponding to RNA-2 of BNYVV isolates were analyzed and Ukrainian isolate AG9 of BNYVV was assigned to type A strains based on DNA sequences. The nucleotide sequence of gene encoding a coat protein of Ukrainian isolate...

Analysis of molecular-genetic polymorphism of barley varieties was performed using AFLP-method. A system for identification and differentiation of barley varieties based on AFLP-markers was developed. Results of testing for 19 varieties indicate a high differential ability of the developed system. Identification of varieties can be conducted using...

Analysis of molecular-genetic polymorphism of barley varieties was performed using the AFLP-method. A system for identification and differentiation of barley varieties based on AFLP-markers was developed. Results of testing of 19 varieties indicate a high differential ability of the developed system. Identification of varieties can be conducted usi...

There is plenty of evidence for genome instability in somatic tissues due to mitotic disorders. This short review focuses on the phenomena of chromosome number reduction in somatic plant cells referred to in the literature by different names as somatic reduction, somatic segregation, meiosis-like reduction, prophase grouping and assortment mitosis.