Myrina Boulais

Myrina Boulais
Université de Bretagne Occidentale | UBO · Laboratoire des Sciences de l’Environnement Marin- LEMAR (UMR 6539)

PhD

About

35
Publications
5,238
Reads
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509
Citations
Citations since 2017
18 Research Items
436 Citations
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Introduction
I am working on examining the effects of natural and anthropologic stressors on the reproduction and various life stages of marine organisms
Additional affiliations
November 2015 - May 2017
University of North Carolina at Wilmington
Position
  • Professor (Associate)
Description
  • I am working on examining the effects of natural and anthropologic stressors on various life stages of shellfish.
May 2015 - October 2015
University of North Carolina at Wilmington
Position
  • PostDoc Position
Description
  • I worked on examining the effects of natural and anthropologic stressors on various life stages of shellfish.
October 2011 - October 2014
Institut Français de Recherche pour l'Exploitation de la Mer
Position
  • PhD Student
Description
  • My research aimed at understanding the mechanisms controlling ATP synthesis in Pacific oyster spermatozoa.

Publications

Publications (35)
Article
Dinoflagellates from the globally distributed genus Alexandrium are known to produce both paralytic shellfish toxins (PST) and uncharacterized bioactive extracellular compounds (BEC) with allelopathic, ichthyotoxic, hemolytic and cytotoxic activities. In France, blooms of Alexandrium minutum appear generally during the spawning period of most bival...
Article
Early life stages (ELS) of numerous marine invertebrates mustcope with man-made contaminants, including plastic debris, during their pelagic phase. Among the diversity of plastic particles, nano-sized debris, known as nanoplastics, can induce effects with severe outcomes in ELS of various biological models, including the Pacific oyster Crassostrea...
Article
Oysters are keystone species that use external fertilization as a sexual mode. The gametes are planktonic and face a wide range of stressors, including plastic litter. Nanoplastics are of increasing concern because their size allows pronounced interactions with biological membranes, making them a potential hazard to marine life. In the present stud...
Article
Harmful algal blooms (HABs) of toxic species of the dinoflagellate genus Dinophysis are a threat to human health as they are mainly responsible for diarrheic shellfish poisoning (DSP) in the consumers of contaminated shellfish. Such contamination leads to shellfish farm closures causing major economic and social issues. The direct effects of numero...
Article
The dinoflagellate genus Alexandrium comprises species that produce highly potent neurotoxins known as paralytic shellfish toxins (PST), and bioactive extracellular compounds (BEC) of unknown structure and ecological significance. The toxic bloom-forming species, Alexandrium minutum, is distributed worldwide and adversely affects many bivalves incl...
Article
Though bivalve mollusks are keystone species and major species groups in aquaculture production worldwide, gamete biology is still largely unknown. This review aims to provide a synthesis of current knowledge in the field of sperm biology, including spermatozoa motility, flagellar beating, and energy metabolism; and to illustrate cellular signaling...
Article
Artificial reproduction involves collection and handling of gametes in a way that secures their quality and maximizes the fertilization outcome. In addition to initial sperm quality, numerous steps can affect the final result of fertilization, from the sperm collection process until gamete mixing (or co-incubation) when the spermatozoon enters or f...
Chapter
The explosion of the Deepwater Horizon (DWH) drilling unit in April 2010 led to the largest marine oil spill in US history. For 87 days, unprecedented amounts of crude oil and dispersant were released into the Gulf of Mexico (GoM), coincident with the spawning and recruitment season of the oyster, Crassostrea virginica. Effects of acute exposures t...
Article
Harmful algal blooms are a threat to aquatic organisms and coastal ecosystems. Among harmful species, the widespread distributed genus Alexandrium is of global importance. This genus is well-known for the synthesis of paralytic shellfish toxins which are toxic for humans through the consumption of contaminated shellfish. While the effects of Alexan...
Article
The explosion of the Deepwater Horizon (DWH) oil drilling rig resulted in the release of crude oil into the Gulf of Mexico. This event coincided with the spawning season of the Eastern oyster, Crassostrea virginica. Although oil bound to sediments constitutes an important source of polycyclic aromatic hydrocarbon (PAH) exposure to benthic organisms...
Article
Full-text available
A predominant concern following oil spills is toxicity to aquatic organisms. However, few data are available on effects in deep‐sea cold water fishes. This study had three major objectives. First, investigate the relative sensitivity of the deep‐sea species Anoplopoma fimbria (sablefish) to acute effects of three aromatic compounds (toluene, 2‐meth...
Article
Full-text available
Investigating the roles of chemical factors stimulating and inhibiting sperm motility is required to understand the mechanisms of spermatozoa movement. In this study, we described the composition of the seminal fluid (osmotic pressure, pH, and ions) and investigated the roles of these factors and salinity in initiating spermatozoa movement in the P...
Article
In brooding mollusc species that incubate larvae before their release in seawater, the biology of gametes remains poorly explored. The present study describes some biological characteristics of sperm of the European flat oyster, a native species that has been over-exploited in the past and is nowadays classified as an endangered species in Europe....
Poster
Full-text available
The effects of moderate and severe acidification on oyster gametogenesis, fertilization, and early larval development successes
Article
Full-text available
Atmospheric carbon dioxide concentrations have been rising during the past century, leading to ocean acidification (OA). Coastal and estuarine habitats experience annual pH variability that vastly exceeds the magnitude of long-term projections in open ocean regions. Eastern oyster (Crassostrea virginica) reproduction season coincides with periods o...
Article
Fertilization of the oocyte is a critical process of sexual reproduction depending among other factors on spermatozoa functionality. Mitochondria participate in many crucial processes for spermatozoa motility and fertilizing ability. The Pacific oyster, Crassostrea gigas, is characterized by a high inter-individual variability of its reproductive s...
Poster
Full-text available
Objectives: • Determine the LC50s of three petroleum hydrocarbons to the Sablefish based on the static renewal test. • Assess if sablefish exhibit sensitivity within the observed range for pelagic species Conclusions: • Results show, that of the three chemicals, phenanthrene was most toxic followed by 2-methylnaphthalene and toluene • Observed LC50...
Article
Full-text available
The Deepwater Horizon (DWH) oil spill in 2010 resulted in the release of millions of liters of oil into the Gulf of Mexico. Dispersants such as Corexit 9500A were used to disperse oil both at the well-head and at the surface. Polycyclic aromatic hydrocarbons and dispersants have been shown to deleteriously affect early life stages of organisms incl...
Article
In the Pacific oyster, spermatozoa are characterized by a remarkably long movement phase (i.e., over 24 h) sustained by a capacity to maintain intracellular ATP level. To gain information on oxidative phosphorylation (OXPHOS) functionality during the motility phase of Pacific oyster spermatozoa, we studied: i) changes in spermatozoa mitochondrial a...
Article
Full-text available
Pacific oyster hatchery production remains highly variable, partially due to inconsistent gamete quality. However, little attention has been paid to oocyte quality in Pacific oyster compared to important vertebrate aquaculture species such as fishes. The aims of the present study were to identify criteria of oocyte and trochophore quality used as p...
Thesis
Cette thèse présente une étude du métabolisme énergétique du spermatozoïde d’huître creuse, Crassostrea gigas. Les principaux objectifs sont (1) d’établir la dynamique des caractéristiques cellulaires et biochimiques du spermatozoïde de cette espèce durant la phase de mouvement, (2) d’identifier les voies de synthèse d’ATP et leurs substrats et (3)...
Article
Full-text available
The Pacific oyster Crassostrea gigas accounts for a large part of shellfish aquaculture production worldwide. Aspects of morphological and functional characteristics of oyster oocytes remain poorly documented, and traditional techniques, such as microscopic observations of shape or fertilization rate, are time and space consuming. The purpose of th...
Poster
Full-text available
The aim of our work was to determine whether pH of SW represents a main characteristic of the medium for activation of the flagellar movement both for testicular (sperm movement activation) and for already activated (sperm movement regulation) Pacific oyster sperm. During oyster natural spawning period, testicular sperm was sampled (2µl) and sperm...
Article
Full-text available
This study is the first demonstration of successful post-thawing development to reproduction stage of diploid cryopreserved larvae in an aquatic invertebrate. Survival, growth and reproductive performances were studied in juvenile and adult Pacific oysters grown from cryopreserved embryos. Cryopreservation was performed at three early stages: troch...
Book
Full-text available
The present chapter aims at describing the main biological characteristics of two oyster species, both important for their commercial values. Oyster spermatozoa belong to the "aquasperm" according to ultra-structural data: they possess an acrosome and four mitochondria. The sperm flagellum is devoid of progressive motility eitheir in the gonads or...
Article
Full-text available
We used a 2-DE proteomic approach to identify abundant proteins linked to oocyte quality in the Pacific oyster Crassostrea gigas, an economically important bivalve. Oocyte quality of 14 females was estimated by recording fertilisation and early developmental success until D-larval stage under controlled conditions. Proteins that were differentially...
Data
Full-text available
Le projet GAMETOGENES vise à améliorer les connaissances des bases physiologiques et génétiques de la reproduction et des métabolismes associés chez un mollusque d'importance économique l'huître creuse, Crassostrea gigas. Il a pour objectifs (1) la définition de marqueurs clés de la gamétogenèse, de l'investissement reproducteur et du déterminisme...
Article
This work assesses the present knowledge on Pacific oyster sperm biology in comparison to two marine fish species (tur-bot and seabass) whose sperm characteristics are well described. Sperm morphology mainly differs by the presence of an acrosome in Pacific oyster which is absent in both fish species. In turbot as in Pacific oyster, a sperm 'matura...
Poster
Full-text available
The Pacific oyster Crassostrea gigas has a very high fecundity. When its gonad is mature, it represents up to 60% of the volume of the visceral mass (Fabioux et al., 2005). High fecundity is associated with high variability of quality of gametes. To better understand factors involved in this variability, we conducted a 2-DE proteomic approach to id...
Poster
Full-text available
Since the first works in the 19th century, techniques used to study fish sperm movement evolved from subjective assessment of the percentage of motile cells up to most recent CASA (Computed Assisted Sperm Analysis) systems. A CASA plug-in for the Image J software, able to measure the commonly used motility parameters of small biological objects, is...

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Project (1)