Myrielle Dupont-Rouzeyrol

Institut Pasteur de Nouvelle-Calédonie, Nouméa, New Caledonia · Unité de Recherche et d'Expertise Dengue et Arboviroses

Zika virus (ZIKV) shows an enigmatic epidemiological profile in Africa. Despite its frequent detection in mosquitoes, few human cases have been reported. This could be due to the low infectious potential or low virulence of African ZIKV lineages. This study sought to assess the susceptibility of A. aegypti and C. quinquefasciatus to ZIKV strains fr...

Objectives There are concerns about the potential effect of social distancing used to control coronavirus disease 2019 (COVID-19) on the incidence of cardiovascular diseases (CVD). Study design Retrospective cohort study Methods We examined the association between lockdown and CVD incidence in a Zero-COVID country, New-Caledonia. Inclusion criteri...

Dengue, Zika and chikungunya viruses cause significant human public health burdens in the world. These arboviruses are transmitted by vector mosquito species notably Aedes aegypti and Aedes albopictus. In the Pacific region, more vector species of arboviruses belonging to the Scutellaris Group are present. Due to the expansion of human travel and i...

First identified in 1947, Zika virus took roughly 70 years to cause a pandemic unusually associated with virus-induced brain damage in newborns. Zika virus is transmitted by mosquitoes, mainly Aedes aegypti, and secondarily, Aedes albopictus, both colonizing a large strip encompassing tropical and temperate regions. As part of the international pro...

First identified in 1947, Zika virus took roughly 70 years to cause a pandemic unusually associated with virus-induced brain damage in newborns. Zika virus is transmitted by mosquitoes, mainly Aedes aegypti , and secondarily, Aedes albopictus , both colonizing a large strip encompassing tropical and temperate regions. As part of the international p...

Background Dengue dynamics result from the complex interactions between the virus, the host and the vector, all being under the influence of the environment. Several studies explored the link between weather and dengue dynamics and some investigated the impact of climate change on these dynamics. Most attempted to predict incidence rate at a countr...

The mosquito Aedes aegypti is the major vector of arboviruses like dengue, Zika and chikungunya viruses. Attempts to reduce arboviruses emergence focusing on Ae. aegypti control has proven challenging due to the increase of insecticide resistances. An emerging strategy which consists of releasing Ae. aegypti artificially infected with Wolbachia in...

Background Biological control programs involving Wolbachia -infected Aedes aegypti are currently deployed in different epidemiological settings. New Caledonia (NC) is an ideal location for the implementation and evaluation of such a strategy as the only proven vector for dengue virus (DENV) is Ae . aegypti and dengue outbreaks frequency and severit...

Dengue virus (DENV) evolutionary dynamics are characterized by frequent DENV genotype/lineage replacements, potentially associated with changes in disease severity and human immunity. New Caledonia (NC) and Cambodia, two contrasted epidemiological settings, respectively experienced a DENV-1 genotype IV to I replacement in 2012 and a DENV-1 genotype...

Zika virus, an arbovirus responsible for major outbreaks, can cause serious health issues, such as neurological diseases. In the present study, different types of samples (serum, saliva, and urine), collected in 2015–2016 in New Caledonia and French Guiana from 53 patients presenting symptoms and clinical signs triggered by arbovirus infections, we...

Since the epidemic in 2007, studies on vector competence for Zika virus (ZIKV) have intensified, showing that the transmission efficiency varies depending on the vector population, ZIKV strain, and dose of the infectious blood meal. In this study, we aimed to investigate the replication of African and Asian ZIKV strains in vitro and in vivo in orde...

Background In 2017, New Caledonia experienced an outbreak of severe dengue causing high hospital burden (4379 cases, 416 hospital admissions, 15 deaths). We decided to build a local operational model predictive of dengue severity, which was needed to ease the healthcare circuit. Methods We retrospectively analyzed clinical and biological parameter...

Compared to the previous 2013-2014 dengue outbreak, the 2016-2017 outbreak in New Caledonia was characterized by an increased number of severe forms associated with hepatic presentations. Some clinical and biological parameters along with a history of previous dengue infection were identified as risk factors predicting the development of severe den...

Zika virus (ZIKV) is a Flavivirus transmitted by Aedes mosquitoes responsible of a worldwide outbreak between 2013 and 2016. However, no ZIKV outbreak has been described in Southeast Asia since 2017. In this study, we report the first microcephaly case with probable ZIKV infection during pregnancy in Lao People’s Democratic Republic.

Background Dengue dynamics result from the complex interaction between the virus, the host and the vector, all being under the influence of the environment. Several studies have explored the link between climate and dengue outbreaks in New Caledonia. None of them have explored the evolution of the dengue outbreak risk facing climate change. Methodo...

Background In 2017, New Caledonia experienced an outbreak of severe dengue causing high hospital burden (4,379 cases, 416 hospital admissions, 15 deaths). We decided to build a local operational model predictive of dengue severity, which was needed to ease the healthcare circuit. Methods We retrospectively analyzed clinical and biological paramete...

Dengue virus (DENV) serotype-2 was detected in the South Pacific region in 2014 for the first time in 15 years. In 2016–2020, DENV-2 re-emerged in French Polynesia, Vanuatu, Wallis and Futuna, and New Caledonia, co-circulating with and later replacing DENV-1. In this context, epidemiological and molecular evolution data are paramount to decipher th...

Background: Mosquito vectors cause a significant human public health burden through the transmission of pathogens. Due to the expansion of international travel and trade, the dispersal of these mosquito vectors and the pathogens they carry is on the rise. Entomological surveillance is therefore required which relies on accurate mosquito species id...

Zika virus (ZIKV) has caused severe epidemics in South America beginning in 2015, following its spread through the Pacific. We comparatively assessed the vector competence of ten populations of Aedes aegypti and Ae. albopictus from Brazil and two of Ae. aegypti and one of Culex quinquefasciatus from New Caledonia to transmit three ZIKV isolates bel...

In New Caledonia (NC), Aedes aegypti is the only proven vector of dengue virus (DENV), which is the most prevalent arbovirosis in NC. Since World War II, the four DENV serotypes have circulated regularly in NC. The epidemiological profile, however, has evolved over the last ten years, with the persistence of DENV-1 circulation and the co-circulatio...

Background The French overseas Territory of the Wallis and Futuna Islands has been affected by several dengue epidemics. Aedes polynesiensis is the main mosquito vector described in this territory. Other Aedes species have been reported, but recent entomological data are missing to infer the presence of other potential arbovirus vectors and to asse...

We synthesized 50 benzimidazole (BMZ) derivatives with 1,2‐phenylenediamines and aromatic aldehydes under mild oxidation conditions utilizing inexpensive, non‐toxic inorganic salt sodium metabisulfite by one‐pot condensation reaction and screened their ability to interfere with Zika virus (ZIKV) infection utilizing a cell‐based phenotypic assay. Se...

Objective: The re-emergence and spreading of tropical viruses to new areas raised worldwide a wave of concern. To treat patients in an early stage and prevent diffusion of the outbreak, an early diagnosis, and thus fast and adequate detection, is needed. To this aim, a multiplex reverse transcription real-time polymerase chain reaction TaqMan® met...

Zika virus (ZIKV) infection is a serious public health concern due to its ability to induce neurological defects and its potential for rapid transmission at a global scale. However, no vaccine is currently available to prevent ZIKV infection. Here, we report the development of a yeast-derived subunit protein vaccine for ZIKV. The envelope protein d...

Arboviruses are viruses transmitted to humans by the bite of infected mosquito vectors. Over the last decade, arbovirus circulation has increasingly been detected in New Caledonia (NC), a French island territory located in the subtropical Pacific region. Reliable epidemiological, entomological, virological and climate data have been collected in NC...

Zika virus (ZIKV) is a Flavivirus that is transmitted to humans by Aedes mosquitoes. ZIKV is divided into two phylogenetic lineages, African and Asian. In the Asian lineage, Pacific and American clades have been linked to the recent worldwide outbreak of ZIKV. The aim of this study was to measure the vector competence of Aedes aegypti for seven ZIK...

Nucleic acid testing during the preseroconversion viremic phase is required to differentially diagnose arboviral infections. The continuing emergence of arboviruses such as Zika (ZIKV), dengue (DENV), and chikungunya (CHIKV) viruses necessitates the development of a flexible diagnostic approach. Similar clinical signs and the priority to protect pr...

Background In 2013, Zika virus (ZIKV) emerged in French Polynesia and spread through the Pacific region between 2013 and 2017. Several potential Aedes mosquitoes may have contributed to the ZIKV transmission including Aedes aegypti, the main arbovirus vector in the region, and Aedes polynesiensis, vector of lymphatic filariasis and secondary vector...

Infection, dissemination, transmission rates and transmission efficiency at 6, 9, 14 and 21 days post-infection (dpi) for Aedes aegypti Pacific populations. (DOCX)

Infection, dissemination, transmission rates and transmission efficiency at 6, 9, 14 and 21 days post-infection (dpi) for Aedes polynesiensis Pacific populations. (DOCX)

Introduction En 2016–17, ce territoire d’outre mer a connu une épidémie de dengue sévère (516 hospitalisations/4486 cas, 15 décès) caractérisé par la cocirculation de 3 sérotypes. L’objectif de l’étude était d’identifier des facteurs de risque de sévérité et de développer un modèle prédictif de risque. Matériels et méthodes Étude rétrospective des...

Based on serological evidence and viral isolation, Zika virus (ZIKV) has circulated for many years relatively benignly in a sylvatic cycle in Africa and an urban cycle in South East Asia (SEA). With the recent availability of limited but novel Indian ZIKV sequences to add to the plethora of SEA sequences, we traced the phylogenetic history and spat...

Zika virus (ZIKV) infection has been associated with neurologic disorders including Guillain-Barré syndrome (GBS). In New Caledonia during the ZIKV outbreak (2014–2015), case-control and retrospective studies have been performed to assess the link between ZIKV and GBS. Among the 15 cases included, 33% had evidence of a recent ZIKV infection compare...

Emerging Microbes & Infections (2017) 6, e111; doi:10.1038/emi.2017.102; published online 13 December 2017

Background: Dengue virus (DENV) is the arbovirus with the highest incidence in New Caledonia and in the South Pacific region. In 2012-2014, a major DENV-1 outbreak occurred in New Caledonia. The only known vector of DENV in New Caledonia is Aedes aegypti but no study has yet evaluated the competence of New Caledonia Ae. aegypti populations to tran...

The global spread and infective complications of Zika virus (ZKV) and Dengue virus (DENV) have made them flaviviruses of public health concern. Serological diagnosis can be challenging due to antibody cross-reactivity, particularly in secondary flavivirus infections or when there is a history of flavivirus vaccination. The virus neutralization assa...

Background Dengue is a mosquito-borne virus that causes extensive morbidity and economic loss in many tropical and subtropical regions of the world. Often present in cities, dengue virus is rapidly spreading due to urbanization, climate change and increased human movements. Dengue cases are often heterogeneously distributed throughout cities, sugge...

Geographical characteristics and incidence rates for all neighborhoods. (DOCX)

Estimation of the area covered by vegetation during the two dengue epidemics. See supplementary information for details. (TIFF)

Dengue incidence rates for each quintile of the variables associated with the 2008–09 (left) and 2012–13 (right) epidemics. Each boxplot represents the first, second and third quartiles of incidence rates across neighborhoods in the quintile of the explanatory variable, whiskers represent 1.5 of the inter-quartile range, empty dots are outliers (ne...

Model validation for 2008–09 and 2012–13 epidemics. (A) Association between observed and predicted incidence rates with regression line (dotted line) and regression coefficient (with 95% CI); the solid line has a slope of 1. (B) Distribution of residuals obtained by subtracting predicted from observed incidence rate and plotted as a histogram. (C)...

Pearson correlation between dependent variables. The lower triangle of the tables shows correlation coefficients whereas the upper triangle shows p-values. (DOCX)

Multivariable model for the 2008–09 epidemic, with data aggregated at the block of houses scale. (DOCX)

Estimation of vegetation coverage and model validation. (DOCX)

Variables used in regression analysis and their quintiles. (DOCX)

Study site. See text for details. (EPS)

Multivariable models for the 2008–09 and 2012–13 epidemics, with variables categorized in terciles. (DOCX)

Introduction Lors de l’epidemie de Zika virus (ZikV) en Nouvelle Caledonie (NC) en 2014 (1500 cas), une augmentation de l’incidence du syndrome de Guillain Barre (SGB) a ete notee. Objectifs Nous avons voulu savoir si cette augmentation d’incidence du SGB etait significative et si la seropositivite au ZikV etait significativement plus importante ch...