Muy-Teck Teh

Muy-Teck Teh
Queen Mary, University of London | QMUL · Centre for Oral Immunology & Regenerative Medicine

BSc, Ph.D.

About

117
Publications
15,736
Reads
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3,238
Citations
Introduction
Transcriptome pattern recognition in human cells and tissues for pre-disease prediction. Aims to identify signatures and key biomarkers to act as "Molecular Seismometer" to predict cell behaviour, enable early diagnosis & personalised therapeutics. https://sites.google.com/view/qmids/home
Additional affiliations
January 2005 - present
Queen Mary, University of London
December 2002 - January 2005
Queen Mary, University of London
Position
  • CRUK Postdoctoral Fellow
Description
  • Wnt Signalling in Basal Cell Carcinoma
May 2000 - December 2002
Queen Mary, University of London
Position
  • Wellcome Trust Postdoctoral Fellow
Description
  • Hedgehog and Forkhead Box Signalling in Basal Cell Carcinomas
Education
September 1996 - March 2000
King's College London
Field of study
  • Physiology
September 1993 - June 1996
King's College London
Field of study
  • Biomedical Science

Publications

Publications (117)
Article
Full-text available
Background: Heterogeneity in oral potentially malignant disorder (OPMD) poses a problem for accurate prognosis that impacts on treatment strategy and patient outcome. A holistic assessment based on gene expression signatures from both the tumour cells and their microenvironment is necessary to provide a more precise prognostic assessment than just...
Article
Full-text available
Nasopharyngeal carcinoma (NPC) is a highly aggressive tumor characterized by distant metastasis. Deletion or down-regulation of the tumor suppressor protein ras-association domain family protein1 isoform A ( RASSF1A ) has been confirmed to be a key event in NPC progression; however, little is known about the effects or underlying mechanism of RASSF...
Article
Sustained expression of FOXM1 is a hallmark of nearly all human cancers including squamous cell carcinomas of the head and neck (HNSCC). HNSCCs partially preserve the epithelial differentiation program, which recapitulates fetal and adult traits of the tissue of tumor origin but is deregulated by genetic alterations and tumor‐supporting pathways. U...
Article
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Background: The concept of head and neck cancers (HNSCC) having unique molecular signatures is well accepted but relating this to clinical presentation and disease behaviour is essential for patient benefit. Currently the clinical significance of HNSCC molecular subtypes is uncertain therefore personalisation of HNSCC treatment is not yet possible...
Article
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Exosomes are extracellular vesicles released by almost all cell types, including cancer cells, into bodily fluids such as saliva, plasma, breast milk, semen, urine, cerebrospinal fluid, amniotic fluid, synovial fluid and sputum.Their key function being intercellular communication with both neighbouring as well as distant cells. Cancer exosomes have...
Article
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Vimentin, a type III intermediate filament protein, is found in most cells along with mi-crofilaments and microtubules. It has been shown that the head domain folds back to associate with the rod domain and this association is essential for filament assembly. The N-terminally tagged vimentin has been widely used to label the cytoskeleton in live ce...
Method
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One step reverse transcription qPCR detail protocol for 384-well plate format.
Article
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Developmental consequences of prenatal drug exposure have been reported in many human cohorts and animal studies. The long-lasting impact on the offspring—including motor and cognitive impairments, cranial and cardiac anomalies and increased prevalence of ADHD—is a socioeconomic burden worldwide. Identifying the molecular changes leading to develop...
Article
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Objective Besides angiotensin converting enzyme 2 (ACE2), an active involvement of proteases (FURIN and/or TMPRSS2) is important for cellular entry of SARS-CoV-2. Therefore, a simultaneous expression profiling of entry proteins in a tissue might provide a better risk assessment of SARS-CoV-2 infection as compared to individual proteins. In an attem...
Article
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Epithelial-mesenchymal transition (EMT) is a reversible plethora of molecular events where epithelial cells gain the phenotype of mesenchymal cells to invade the surrounding tissues. EMT is a physiological event during embryogenesis (type I) but also happens during fibrosis (type II) and cancer metastasis (type III). It is a multifaceted phenomenon...
Article
Background: Nicotine withdrawal syndrome is a major clinical problem. Animal models with sufficient predictive validity to support translation of pre-clinical findings to clinical research are lacking. Aims: We evaluated the behavioural and neurochemical alterations in zebrafish induced by short- and long-term nicotine withdrawal. Methods: Zeb...
Article
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An interactive effect between nicotine and 3,4-methylenedioxymethamphetamine (MDMA) has been reported but the mechanism underlying such interaction is not completely understood. This study used zebrafish to explore gene expression changes associated with altered sensitivity to the rewarding effects of MDMA following 2-week exposure to nicotine and...
Article
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Oral cancer is a sub-category of head and neck cancers that primarily initiates in the oral cavity. The primary treatment option for oral cancer remains surgery but it is associated with massive disfigurement, inability to carry out normal oral functions, psycho-social stress and exhaustive rehabilitation. Other treatment options such as chemothera...
Article
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To facilitate smoking genetics research we determined whether a screen of mutagenized zebrafish for nicotine preference could predict loci affecting smoking behaviour. From 30 screened F3 sibling groups, where each was derived from an individual ethyl-nitrosurea mutagenized F0 fish, two showed increased or decreased nicotine preference. Out of 25 i...
Article
Full-text available
To facilitate smoking genetics research we determined whether a screen of mutagenized zebrafish for nicotine preference could predict loci affecting smoking behaviour. From 30 screened F3 sibling groups, where each was derived from an individual ethyl-nitrosurea mutagenized F0 fish, two showed increased or decreased nicotine preference. Out of 25 i...
Article
Full-text available
To facilitate smoking genetics research we determined whether a screen of mutagenized zebrafish for nicotine preference could predict loci affecting smoking behaviour. From 30 screened F3 sibling groups, where each was derived from an individual ethyl-nitrosurea mutagenized F0 fish, two showed increased or decreased nicotine preference. Out of 25 i...
Article
Full-text available
Abnormal keratinocyte differentiation is fundamental to pathologies such as skin cancer and mucosal inflammatory diseases. The ability to grow keratinocytes in vitro allows the study of differentiation however any translational value is limited if keratinocytes get altered by the culture method. Although serum lipids (SLPs) and phenol red (PR) are...
Article
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Desmoglein 3 (Dsg3) plays a crucial role in cell-cell adhesion and tissue integrity. Increasing evidence suggests that Dsg3 acts as a regulator of cellular mechanotransduction, but little is known about its direct role in mechanical force transmission. The present study investigated the impact of cyclic strain and substrate stiffness on Dsg3 expres...
Preprint
Full-text available
Desmoglein 3 (Dsg3), plays a crucial role in cell-cell adhesion and tissue integrity. Increasing evidence suggests that Dsg3 acts as a regulator of cellular mechanotransduction, but little is known about its direct role in mechanical force transmission. The present study investigated the impact of cyclic strain and substrate stiffness on Dsg3 expre...
Article
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Desmoglein-3 (Dsg3), the Pemphigus Vulgaris (PV) antigen (PVA), plays an essential role in keratinocyte cell–cell adhesion and regulates various signaling pathways involved in the progression and metastasis of cancer where it is upregulated. We show here that expression of Dsg3 impacts on the expression and function of p53, a key transcription fact...
Article
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Keratin 15 (K15), a type I keratin, which pairs with K5 in epidermis, has been used extensively as a biomarker for stem cells. Two commercial antibodies, LHK15, a mouse monoclonal and EPR1614Y, a rabbit monoclonal, have been widely employed to study K15 expression. Here we report differential reactivity of these antibodies on epithelial cells and t...
Preprint
Full-text available
BACKGROUND: Although there is clear evidence of genetic contributions to susceptibility to nicotine addiction, it has proved difficult to identify causal alleles and pathways from studies in humans. Mutagenesis in model species generates strong phenotypes not present in wildtype populations and can be used to identify biological mechanisms underlyi...
Preprint
Full-text available
Desmoglein-3 (Dsg3), the Pemphigus Vulgaris (PV) antigen (PVA), plays an essential role in keratinocyte cell-cell adhesion and regulates various signaling pathways implicated in the pathogenesis the PV blistering disease. We show here that expression of Dsg3 may directly influence p53, a key transcription factor governing the response to cellular s...
Article
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Uncontrolled HH/GLI and WNT/β-catenin signaling are important events in the genesis of many cancers including skin cancer and are often implicated in tumour progression, invasion and metastasis. However, due to the complexity and context-dependency of both pathways, little is known about HH and WNT interactions in human carcinogenesis. In the curre...
Article
Background: Cancer-associated fibroblasts (CAFs) were shown to be important for tumour progression in head and neck squamous cell carcinomas (HNSCCs). Their heterogeneity and lack of specific markers is increasingly recognized. Integrin α11 was recently shown to be expressed by CAFs and might serve as a specific CAF marker. Aim: To investigate i...
Article
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The forkhead box M1 (FOXM1) transcription factor gene has been implicated in almost all human cancer types. It would be an ideal biomarker for cancer detection but, to date, its translation into a cancer diagnostic tool is yet to materialise. The quantitative Malignancy Index Diagnostic System (qMIDS) was the first FOXM1 oncogene-based diagnostic t...
Article
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Cellular and molecular processes that mediate individual variability in impulsivity, a key behavioral component of many neuropsychiatric disorders, are poorly understood. Zebrafish heterozygous for a nonsense mutation in ache (ache (sb55/+)) showed lower levels of impulsivity in a 5-choice serial reaction time task (5-CSRTT) than wild type and ache...
Article
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Diagnosing oral cancer has been a tremendous challenge due to its anatomical complexity and tumour heterogeneity. This Editorial article discuss the challenges and potential answers to oral cancer diagnosis using a recent multi-gene molecular diagnostic test - the quantitative Malignancy Index System (qMIDS).
Article
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Background: Altered expression of S100A16 has been reported in human cancers, but its biological role in tumorigenesis is not fully understood. This study aimed to investigate the clinical significance and functional role of S100A16 in oral squamous cell carcinoma (OSCC) suppression. Methods: S100A16 mRNA and/or protein levels were examined by q...
Article
Full-text available
Citation: Teh MT. Is salivary exosome the answer to early detection of oral can-cer? Dent Open J. 2015; 2(2): e3-e4.
Article
Exposure to alcohol during early central nervous system development has been shown variously to affect aspects of physiological and behavioural development. In extreme cases, this can extend to craniofacial defects, severe developmental delay and mental retardation. At more moderate levels, subtle differences in brain morphology and behaviour have...
Article
Full-text available
Expert molecular biologist and cancer researcher Dr Muy-Teck Teh shares his opinions on the field and gives details of his work towards developing early warning systems for head and neck cancer. A brief account of the importance of FOXM1 in the field of cancer research and its clinical translational impact.
Article
Full-text available
Abnormal Sonic Hedgehog signalling leads to increased transcriptional activation of its downstream effector, glioma 2 (GLI2), which is implicated in the pathogenesis of a variety of human cancers. However, the mechanisms underlying the tumorigenic role of GLI2 remain elusive. We demonstrate that overexpression of GLI2-β isoform, which lacks the N-t...
Article
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Epigenetic reprogramming of the methylome has been implicated in all stages of cancer evolution. It is now well accepted that cancer cells exploit epigenetic reprogramming, a mechanism that regulates stem/progenitor cell renewal and differentiation, to promote cancer initiation and progression. The oncogene FOXM1 has been implicated in all major fo...
Article
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Keratin 15 (K15) is type I keratin protein co-expressed with the K5/K14 pair present in the basal keratinocytes of all stratified epithelia. Although it is a minor component of the cytoskeleton with a variable expression pattern, nonetheless its expression has been reported as a stem cell marker in the bulge of hair follicles. Conversely, suprabasa...
Article
Full-text available
Histopathological discordance with molecular phenotype of many human cancers poses clinically challenging tasks for accurate cancer diagnosis which impacts on treatment strategy and patient outcome. Hence, an objective, accurate and quantitative method is needed. A quantitative Malignancy Index Diagnostic System (qMIDS) was developed based on 14 FO...
Article
Full-text available
A decade since the first evidence implicating the cell cycle transcription factor Forkhead Box M1 (FOXM1) in human tumorigenesis, a slew of subsequent studies revealed an oncogenic role of FOXM1 in the majority of human cancers including oral, nasopharynx, oropharynx, esophagus, breast, ovary, prostate, lung, liver, pancreas, kidney, colon, brain,...
Chapter
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The cell cycle transcription factor FOXM1 has been found to be ubiquitously upregulated in a majority of human cancers implicating a fundamental role for FOXM1 in tumourigenesis. Its dose-dependent expression pattern correlated well with tumour progression starting from cancer predisposition and initiation, early premalignancy and progression, to m...
Article
Full-text available
Keratin 15 (K15) is a type I keratin that is used as a marker of stem cells. Its expression is restricted to the basal layer of stratified epithelia, and the bulge in hair follicles. However, in certain clinical situations including oral lichen planus, K15 is induced in suprabasal layers, which is inconsistent with the role of a stem cell marker. T...
Data
Induction of PKCδ and PKCη transcription in suspension culture. N-Terts grown in SFM were suspended in DMEM +10% FCS containing 1.3% (w/v) methylcellulose. After 24 h the cells were harvested to determine the mRNA levels of PKCα, PKCδ, PKCη, PKCζ and PKCε by qPCR. Each bar represents the mean±SEM where n = 3. (P<0.01, very significant, **; P<0.001,...
Data
Differentiation of primary human keratinocytes in suspension. (a) N-Terts and (b) normal primary human epidermal keratinocytes growing in RM+ +10% FCS were suspended in DMEM +20% FCS containing 1.3% (w/v) methylcellulose. At different time intervals, cells were harvested and used to determine mRNA expression by qPCR. The RM+ medium contains 10 ng/m...
Data
Induction of K15 transcription in HaCaT at high cell density. (a) HaCaT keratinocytes were grown at low (30%) and high (95%) confluence at low calcium (0.09 mM) concentration for 24 h after which the cells were lysed to determine the mRNA expression. (b) The cells grown at low (S) and high (C) densities were analyzed by western blotting for express...
Data
Oligonucleotides used in this study. (TIF)
Article
Full-text available
The oncogene FOXM1 has been implicated in all major types of human cancer. We recently showed that aberrant FOXM1 expression causes stem cell compartment expansion resulting in the initiation of hyperplasia. We have previously shown that FOXM1 regulates HELLS, a SNF2/helicase involved in DNA methylation, implicating FOXM1 in epigenetic regulation....
Data
Full-text available
Absolute qPCR primers. (A) Nucleotide sequence of the bisulfite treated promoter region of p16INK4A and their respective primer sequences used in this study. Details of qPCR conditions were performed according to published methods [25], [26]. (B) qPCR primer sequences of the 30 candidate FOXM1-induced differentially methylated genes. Colour shaded...
Article
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J Oral Pathol Med (2012) 41: 454–462 Oral submucous fibrosis (OSMF) is associated with paan chewing, altered collagen metabolism, inflammation and the upregulation of numerous cytokines. OSMF fibroblasts accumulate senescent cells at an increased rate because of increased reactive oxygen species production and DNA double-strand breaks (DDBs), gener...
Patent
Full-text available
Provided are methods for diagnosing cancer in a patient or for identifying a patient at risk of developing cancer. The methods comprise determining the amount of five or more biomarkers selected from HOXA7, AURKA, NEK2, FOXM1B, CCNB1, CEP55, CENPA, DNMT3B, DNMT1, HELLS, MAPK8, BMI1, ITGB1, IVL and CTNNB1 in a sample obtained from a patient and comp...
Conference Paper
Full-text available
Objectives: HOXA7 is a homeobox-containing protein that is known to play a crucial role during embryonic development. Studies from this laboratory have shown that HOXA7 is induced in oral cancer, however, its exact roles are not understood. We have genetically modified a human oral keratinocyte cell line T103C to overexpress a c-myc tagged HOXA7 cD...