Mustapha Missbah El Idrissi

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• Professor
• Rhizobia-legumes interactions at Mohammed V University

Bradyrhizobium sp. RDM4 is a symbiotic nitrogen-fixing bacterium, isolated from root nodules of the Moroccan endemic shrub Retama dasycarpa grown in Moroccan forest soils. In this work, we present the 8.4 Mb draft genome of this strain, characterized by a GC content of 63% and the presence of 8,141 total genes, with 7,032 protein-coding.

In easter Morocco, the abandoned Zaida mine tailings are a real threat to the environment due to the presence of high concentrations of heavy metals, including lead and zinc. To mitigate heavy metals in this area, a phytostabilization strategy was proposed, utilizing native legume species and their associated microsymbionts. To this purpose, 40 bac...

Genista monspessulana is a wild legume of high fodder value in northern Morocco, where it contributes to livestock feeding, particularly during the lean season. The plant fixes nitrogen in symbiosis with soil bacteria known as rhizobia. To identify and characterize its symbiotic partners, we isolated twenty-two bacteria inhabiting the plant nodules...

In this study, we sequenced the genome of Mesorhizobium sp. strain AaZ16, a nitrogen-fixing rhizobial species isolated from the root nodules of Astragalus armatus growing wild in a lead- and zinc-rich mine tailings in the High Atlas, Morocco. This study reveals the genomic characteristics of the root microsymbiont.

In this study, we present the complete genome of Bradyrhizobium lupini LLZ14, a nodule-forming bacterium isolated from Lupinus luteus root nodules with high plant growth-promoting abilities. This genome contains genes predicted to be involved in plant stress tolerance and growth promotion, including auxin production, phosphatase, and 1-aminocyclopr...

Some shrubby legumes represent an appropriate alternative to reduce forest degradation and also an ecologically sustainable strategy that respects the environment, protects against desertification, and improves soil quality and fertility. Retama species are generally used to prevent desertification due to their aptitude to elicit symbiotic nitrogen...

Three bacterial strains, 1AS14IT, 1AS12I and 6AS6, isolated from root nodules of Acacia saligna, were characterized using a polyphasic approach. Phylogenetic analysis based on rrs sequences placed all three strains within the Rhizobium leguminosarum complex. Further phylogeny, based on 1 756 bp sequences of four concatenated housekeeping genes (rec...

Retama dasycarpa is an endemic shrubby leguminous plant of Morocco used in traditional folk medicine. The plant has never been studied for either its phytochemical or pharmacological properties. This study represents the first investigation of the phytochemical profile as well as the antioxidant, the antibacterial, the analgesic effects and the ora...

In this work, we analyzed the effect of water stress and acid pH on the growth of the endemic fodder legume Chamaecytisus albidus, inoculated with four strains of Bradyrhizobium, from three different symbiovars previously isolated from the plant grown in different eco-geographical areas of Morocco. We also assessed the competitiveness of the three...

Currently, salinization is impacting more than 50% of arable land, posing a significant challenge to agriculture globally. Salt causes osmotic and ionic stress, determining cell dehydration, ion homeostasis, and metabolic process alteration, thus negatively influencing plant development. A promising sustainable approach to improve plant tolerance t...

Nitrogen-fixing Bradyrhizobium species are Gram-negative soil bacteria belonging to the Bradyrhizobiaceae family. These bacteria provide a wide range of services and benefits to many legume and nonlegume plants, and in turn, they receive reduced carbon and other metabolites provided by the host plant. The most discussed ability is nitrogen fixation...

In semi-arid to arid climates, soils generally have a low nutrient content, suffer from severe erosion and desertification, and undergo intensive agricultural practices and continued human activities, including overgrazing and mining. To improve low crop yield in those deteriorated soils, farmers generally use agrochemicals such as pesticides and c...

The mining activity around the world produces huge amounts of wastes usually deposited in the open-air as tailings’ dams with no rehabilitation strategies, which menaces the health of the neighboring villages’ populations and the environment. The use of heavy metal-tolerant plants as a cover around the mining areas contributes to attenuate such ris...

Introduction Increasing global warming has made heat stress a serious threat to crop productivity and global food security in recent years. One of the most promising solutions to address this issue is developing heat-stress-tolerant plants. Hence, a thorough understanding of heat stress response mechanisms, particularly molecular ones, is crucial....

The Moroccan endemic Vachellia gummifera grows wild under extreme desert conditions. This plant could be used as an alternative fodder for goats, and camels, in order to protect the Argan forests against overgrazing in Central and Southwestern Moroccan semi-arid areas. With the aim to improve the V. gummifera population's density in semi-arid areas...

Background and aimsIn Morocco's semi-arid and sub-humid climates, the fodder legume Lupinus luteus is cultivated for its high economic and ecological value. In this work, we characterized some microsymbionts of L. luteus isolated by trapping from plants grown in soils of the agricultural area of Zaer, Morocco.Methods The phenotypic and genotypic di...

Arbuscular mycorrhizal fungi (AMF) are obligate symbionts that form a mutualistic symbiosis with a wide variety of plant species including legumes. Lathyrus cicera (red pea), is an important annual legume crop cultivated in several drought-prone areas of the world. Limited information is available on AMF diversity associated with red pea and agricu...

Three bacterial strains, 1AS11 T , 1AS12 and 1AS13, members of the new symbiovar salignae and isolated from root nodules of Acacia saligna grown in Tunisia, were characterized using a polyphasic approach. All three strains were assigned to the Rhizobium leguminosarum complex on the basis of rrs gene analysis. Phylogenetic analysis based on 1734 nuc...

Retama dasycarpa is an endemic Retama species native to the cold semi-arid bioclimates of the High Atlas Mountains in Morocco. In this work, we analyzed the diversity of the microsymbionts nodulating this plant and their different phenotypic and symbiotic characteristics. Phylogenetic analysis of the 16S rRNA gene revealed that the tested isolates...

Aims In Morocco, the Zaida abandoned mine is a major source of metallic trace elements that are harmful to the environment and human health. With the aim to use the heavy metal tolerant legume tree Robinia pseudoacacia in the phytostabilization of this mine’s tailings, we isolated and characterized its indigenous microsymbionts, and tested their ef...

Ononis repens is a legume of the Fabaceae family with great ecological and medicinal importance that grows wild in the Maamora cork oak forest (Rabat, Morocco). The aim of this work was to analyze the diversity, phylogeny, and plant growth-promoting features of the plant root nodules microsymbionts. Out of twenty nodC-containing rhizobial bacteria,...

Conventional rhizobia which are widely distributed in soils worldwide and which include the fixed name “rhizobium” in the genus nomenclature, plus a prefix, such as Rhizobium, Bradyrhizobium, Ensifer, Mesorhizobium, Azorhizobium, Allorhizobium, Neorhizobium, and Pararhizobium Unconventional rhizobia which are not found in all soils, in some legumes...

Acacia saligna is an invasive alien species that has the ability to establish symbiotic relationships with rhizobia. In the present study, genotypic and symbiotic diversity of native rhizobia associated with A. saligna in Tunisia were studied. A total of 100 bacterial strains were selected and three different ribotypes were identified based on rrs...

Out of 70 bacterial strains isolated from root nodules of Lupinus albus and L. angustifolius grown in the soils from the Maamora forest in Morocco, 56 isolates possessed the nodC symbiotic gene, as determined by nodC-PCR, and they were able to renodulate their original hosts. The phenotypic analysis showed that many strains had great potential for...

Sulla flexuosa is a protein-rich Mediterranean pastoral and forage legume that is widely used for animal feed. In this work, we analyzed the diversity of the plant as well as the phylogenetic affiliations of its microsymbiotes. To our knowledge, this is the first evaluation of Sulla natural ecotypes in Morocco. We found that the plant populations c...

Background Mining activity in the Touissit district of Eastern Morocco has led to an unprecedented accumulation of heavy metals, mainly lead and zinc, in the tailing ponds of the open-air mines. This poses a real danger to both the environment and local population. Objectives The goal of this work was to characterize the Plant Growth Promoting Rhi...

In this work, we analyzed the symbiotic performance and diversity of rhizobial strains isolated from the endemic shrubby legume Chamaecytisus albidus grown in soils of three different agroforestry ecosystems representing arid and semi-arid forest areas in Morocco. The analysis of the rrs gene sequences from twenty-four representative strains select...

Trigonella foenum-graecum is a medicinal and culinary plant with varied health benefits. The improvement of its growth in weak soils depends on symbiotic soil bacteria called rhizobia which contribute to the autonomy of legumes towards nitrogen. Biological nitrogen fixation is one of the most beneficial processes for legumes. This process allows the p...

In this work, we analyzed the diversity of the nodule-forming bacteria associated with Lupinus luteus and Lupinus cosentinii grown in the Maamora Cork oak forest acidic soils in Morocco. The phenotypic analysis showed the high diversity of the strains nodulating the two lupine's species. The strains were not tolerant to acidity or high alkalinity....

In the Moroccan Middle Atlas, the tailings rich in lead and other metal residues, in the abandoned Zaida mining district, represent a real threat to environment and the neighboring villages’ inhabitants’ health. In this semi-arid to arid area, phytostabilisation would be the best choice to limit the transfer of heavy metals to populations and groun...

Lotus spp. are a plant of great importance as a forage to improve pastures, to restore degraded soils and the inoculation with best rhizobial strains is widely used in many countries. The aim of this work was to analyze the phylogeny of bacteria nodulating Lotus creticus collected from sandy alkaline saline soil in Tangier province Northern Morocco...

Out of 54 isolates from root nodules of the Moroccan-endemic Chamaecytisus albidus plants growing in soils from the Maamora cork oak forest, 44 isolates formed nodules when used to infect their original host plant. A phenotypic analysis showed the metabolic diversity of the strains that used different carbohydrates and amino acids as sole carbon an...

The aim of this work was to characterize and identify some bacteria isolated from the root nodules of Retama monosperma grown in Sidi Boubker lead and zinc mine tailings. Very few root nodules were obtained on the root nodules of R. monosperma grown in these soils. The three bacteria isolated from the root nodules were tolerant in vitro to differen...

The genetic diversity of bacterial populations’ nodulating Retama sphaerocarpa grown in the soils of Maamora cork forest (Morocco) was examined. ERIC-PCR fingerprinting of 30 strains distributed them in 2 groups, of which a representative strain from each group was studied by multilocus sequence analysis of the 16S rRNA, atpD, and gyrB genes. The t...

Aims: To identify the bacteria nodulating Sulla spinosissima growing profusely in a lead and zinc mine tailings in Eastern Morocco. Methods and results: Thirty two rhizobial cultures, isolated from root nodules of S. spinosissima growing in soils of the mining site, were tolerant to different heavy metals. The ERIC-PCR fingerprinting analysis cl...

In this work we analyzed the diversity of seventy-six bacteria isolated from Pea and faba bean nodules in two regions of Morocco. The molecular diversity was realized using the analysis of the sequences of 16S rRNA and six housekeeping genes (recA, glnII, atpD, dnaK, rpoB and gyrB) and two symbiotic genes (nodA and nodC). The phylogeny of the 16S r...

Aim: To analyze the diversity of nodule-forming bacteria isolated from Lupinus cosentinii naturally-grown in the Maamora cork oak forest (Rabat, Morocco). Methods and results: Out of a total of thirty one bacterial strains, four were selected based on their REP-PCR fingerprinting that were studied by sequencing and phylogenetic analysis of their...

Millions tons of lead and zinc wastes from the abandoned Touissit mine are stored in the open air as dikes in the vicinity of the villages in Eastern Morocco and pose a real danger to both the environment and local populations. To prevent the movement of minerals to the nearby villages and limit the damages to the environment and health, we propose...

Millions tons of lead and zinc wastes from the abandoned Touissit mine are stored in the open air as dikes in the vicinity of the villages in Eastern Morocco and pose a real danger to both the environment and local populations. To prevent the movement of minerals to the nearby villages and limit the damages to the environment and health, we propose...

The purpose of this work was to study the genetic diversity of the nodule-forming bacteria associated with native populations of Vachellia gummifera growing wild in Morocco. The nearly complete 16S rRNA gene sequences from three selected strains, following ARDRA and REP-PCR results, revealed they were members of the genus Ensifer and the sequencing...

Astragalus algarbiensis is a wild herbaceous legume growing in Maamora, the most important cork oak forest in northern Africa. It is a plant of great importance as fodder in silvopastoral systems, and in the restoration of poor and degraded soils. The purpose of this study was to describe the biodiversity of rhizobia nodulating this plant and deter...

This book summarizes the main outcomes and results of the project implementation in one of the regions with higher expected development worldwide. The first part is focused on comparative overviews and technical reports, including the analysis of national LLL legislation in the Mediterranean region, existing LLL practices on the participating count...

This book summarizes the main outcomes and results of the project implementation in one of the regions with higher expected development worldwide. The first part is focused on comparative overviews and technical reports, including the analysis of national LLL legislation in the Mediterranean region, existing LLL practices on the participating count...

A total of 51 bacterial strains were isolated from root nodules of Scorpiurus muricatus sampled from 6 regions of western Algeria. Strain diversity was assessed by rep-PCR amplification fingerprinting, which grouped the isolates into 28 different clusters. Partial nucleotide sequencing of the 16S rRNA gene and BLAST analysis revealed that root nodu...

Abstract In this work, different physical and chemical pretreatments (boiling water, cold stratification and scarification by pure sulfuric acid) were applied to improve the germination rate of Medicago arborea seeds). We also evaluated the influence of different incubation temperatures and different concentrations of NaCl on the physiological germ...

A Polyphasic characterization of 30 bacteria isolated from root nodules of Medicago arborea (Medic tree) plants growing in 3 arid soils of the arid northeast region of Morocco was performed. The phenotypic, symbiotic, and cultural characteristics analyzed allowed the description of a broad physiological diversity among the isolates. The phenogram o...

This volume of the Trilogy of Traditional Foods, part of the ISEKI Food Series, describes important aspects of the production of foods and beverages from all over the globe. The intention of this volume is to provide readers with an appreciation of how products were initially made, and which factors have shaped their development over time. Some mod...

Polyphasic characterization of 61 bacteria isolated from root nodules of Medicago arborea (Medic tree) plants growing in 4 arid soils of the arid eastern area of Morocco was studied. All the isolates characterized were fast growers. The phenotypic, symbiotic, and cultural characteristics analyzed allowed the description of a broad physiological div...

Astragalus gombiformis is a desert symbiotic nitrogen-fixing legume of great nutritional value as fodder for camels and goats. However, there are no data published on the rhizobial bacteria that nodulate this wild legume in northern Africa. Thirty-four rhizobial bacteria were isolated from root nodules of A. gombifomis grown in sandy soils of the S...

On Sep 30, 2013 this sequence version replaced gi:468644591.