Mustafa Usta

Mustafa Usta
Yuzuncu Yil University · Department of Plant Protection

PhD

About

57
Publications
7,273
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244
Citations
Citations since 2017
32 Research Items
179 Citations
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201720182019202020212022202301020304050
201720182019202020212022202301020304050
201720182019202020212022202301020304050

Publications

Publications (57)
Article
Full-text available
Phytoplasma-like symptoms of leaf yellowing and calyx malformation were observed in eggplant (Solanum melongena L.), upward leaves and fruit malformation in pepper (Capsicum annuum L.), and aerial tuber formation in potato (S. tuberosum L.) during the survey performed in the late season (August to September) of 2015 and 2016 in Van province (Turkey...
Article
Objective: The objective of this study was to investigate the group/subgroup of phytoplasma agent in peppers showing phytoplasma symptoms. Material and Methods: In this study, plants collected from Iğdır province in 2020 were analyzed using direct and nested PCR tests, and BLASTn, iphyClassifier, Mega 7, and pDRAW32 programs were used. Results: In...
Conference Paper
Full-text available
2020 yılında Van ilinin İpekyolu ve Gürpınar bölgelerinde açık alanda yetiştirilen hıyar bitkilerinde fitoplazma şüphesi belirtiler gözlemledik. Başlıca hastalık belirtileri anormal ve küçük yapraklar, çiçek anormallikleri, cadı süpürgesi, meyveden akıntı ve rozetleşmedir. Genomik DNA izolasyonu için iki simptomlu ve iki simptomsuz bitki yaprağı ol...
Article
Potato virus S (PVS) is one of the common viruses worldwide and usually produces moderate symptoms in potato plants. The interactions of viral proteins with their hosts are very important in establishing a successful infection and ensuring systemic spread. In this study, the interaction between the PVS coat protein (CP) and the PSH-RGH6 protein in...
Article
Full-text available
Alfalfa mosaic virus (AMV) is one of the most important viral diseases of alfalfa plant among the forage crop, causing significant annual economic losses. The agent is also of potential importance to other cultivars such as tomatoes, potatoes, and peppers in most cases. The identification and phylogenetic relationships of AMV were carried out by re...
Article
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Ribosome-inactivating proteins (RIPs) are one of the enzymes that inhibit protein synthesis after depurination of a specific adenine in ribosomal RNA. The tritin is one of type I RIPs that include RNA-N glycosidase domain from RIP family. In the present study, cDNA encoding tritin from leaves of wheat Kutluk-94 cultivar was isolated and cloned into...
Chapter
Full-text available
Günümüze kadar birçok RIP, bilim insanının ilgisini çekmeyi başarmış ve yaptıkları çalışmalarla geniş spektrumlu antiviral, antifungal, antibakteriyel ve antitümöral aktivite sergilediği bildirilmiştir (Bian vd., 2010). Bunun yanı sıra in vitro ve transgenik bitkilerde de RIP’ler ile benzer şekilde başarı sağlanmıştır (Akkouh vd., 2015). RIP’lerin...
Article
Full-text available
Survey studies were carried out in in 2018 in Adıyaman, Turkey, where tobacco is traditionally produced, to detect the viruses that infect tobacco including Tobacco mosaic tobamovirus (TMV), Cucumber mosaic cucumovirus (CMV), Potato po-tyvirus Y (PVY), Tobacco vein banding mosaic po-tyvirus (TVBMV), and Tobacco etch potyvirus (TEV). Accordingly, 45...
Article
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Tomato (Solanum lycopersicum) is one of the most financially important vegetable crops. It is a species belonging to the Solanaceae family and is cultivated in many countries, including Turkey. The natural presence of ‘Candidatus Phytoplasma solani’ (‘Ca. P. solani’) from the Stolbur group (16SrXII) in tomato plants is extensively characterized bas...
Article
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The natural occurrence of 'Candidatus Phytoplasma trifolii' in pear trees (Pyrus communis Linnaeus) is reported here for the first time. In 2017, a total of thirty-five pear trees, two of them exhibiting leaf rolling along the midvein, reddening, bushy appearance, and upright growth symptoms were sampled in different locations in Van province, Turk...
Article
Full-text available
In order to assess the occurrence and the incidence of Barley yellow dwarf viruses (BYDV-PAV, BYDV-SGV, CYDV-RPV, BYDV-MAV, BYDV-RMV), Wheat streak mosaic virus (WSMV), and Soil-borne wheat mosaic virus (SBWMV) in eastern Anatolia, a survey was conducted in wheat fields from 10 major wheat-growing provinces in Turkey from April 2012 to June 2013. A...
Article
Full-text available
Wheat (Triticum aestivum L.) is a natural host of many viruses. Yellow dwarf viruses belonging to the Luteoviridae family are important virus species that cause economic loss by restricting wheat production worldwide. Surveys were conducted in 2017 to determine Yellow dwarf viruses (BYDV-PAV, BYDV-MAV, BYDV-SGV, BYDV-RMV, and CYDV-RPV) and their in...
Article
Full-text available
Cucumber mosaic virus (CMV) has a broad plant-host range and a wide ecological zone distribution. Virus-like symptoms were observed on tobacco fields of Adiyaman province (Turkey) showing conspicuous mottling, greenish mosaic patterns and severe malformations of leaves. A total of forty tobacco samples tested positive against CMV by reverse transcr...
Article
Full-text available
Nuclear ribosomal DNA (nrDNA) sequence data of the Cuscuta genus, which have been considered as one of the most popular sequences for phylogenetic inference in plants, have been studied from a phylogenetic perspective in agricultural and non-agricultural lands of Turkey. The samples of Cuscuta spp. were collected from different geographical regions...
Article
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The tomato plant exhibiting leaf rolling, witches’ broom, distorted and elongated flower’s sepals in Iğdır province, Turkey, was observed. Total DNA extraction was performed from the symtomatic fresh tomato sample. All DNAs were subjected to Direct and Nested polymerase chain reaction (PCR) with universal primer sets that amplified the 16S rRNA of...
Article
Full-text available
To ascertain the presence of Barley/Cereal yellow dwarf viruses (BYDV-PAV, MAV, SGV, RMV, and CYDV-RPV), a total of 365 wheat leaf samples were randomly collected from wheat cultivation fields of Diyarbakir province in 2016 and were screened by using multiplex RT-PCR and RT-PCR methods. As a result of PCR tests the wheat samples were found to be in...
Article
To ascertain the presence of Barley/Cereal yellow dwarf viruses (BYDV-PAV, MAV, SGV, RMV, and CYDV-RPV), a total of 365 wheat leaf samples were randomly collected from wheat cultivation fields of Diyarbakir province in 2016 and were screened by using multiplex RT-PCR and RT-PCR methods. As a result of PCR tests the wheat samples were found to be in...
Article
Full-text available
Bouganin antiviral protein (BAP) gene, one of the ribosome inactivating proteins, isolated from Bougainvillea spectabilis Willd. was cloned, expressed and the antiviral and antifungal activities were investigated. The full-length bouganin antiviral protein gene was amplified by reverse transcription-PCR using mRNA as template extracted from mature...
Article
Tomato plants displaying severe fruit deformation, flower sterility, aerial rooting, purplish leaves and leaf rolling were observed in tomato fields at Van province (Turkey). Samples were collected, and total DNA was extracted from symptomatic and asymptomatic plants. Nested polymerase chain reaction (nested-PCR) assays were performed to amplify 16...
Article
Full-text available
To ascertain the presence of Barley/Cereal yellow dwarf viruses (BYDV-PAV, MAV, SGV, RMV, and CYDV-RPV), a total of 365 wheat leaf samples were randomly collected from wheat cultivation fields of Diyarbakir province in 2016 and were screened by using multiplex RT-PCR and RT-PCR methods. As a result of PCR tests the wheat samples were found to be in...
Article
Full-text available
Pokeweed antiviral protein (PAP) of Phytolacca americana L. (pokeweed) is a single-chain ribosome-inactivating protein (RIP) characterized by its ability to depurinate plant ribosomes. Here, we isolated, cloned, and expressed the ribosome inactivating protein (RIP) gene, designated as pokeweed antiviral protein type 1 (PAP I), from the summer leave...
Article
Full-text available
Phytoplasma-like symptoms were observed in cucumbers (Cucumis sativus L.) in Van province of Turkey. The major symptoms observed were severe dwarfing, witches’ broom, rosetting, little leaf, and sterility of plants. Genomic DNA of 8 symptomatic and non-symptomatic plant leaves was isolated for the detection of pathogenic DNA. Of the 8 cucumber leaf...
Article
Full-text available
Dodder (Cuscuta sp.) is an obligate parasitic plant that is very difficult to control. In plants the internal transcribed spacer (ITS) region of the 18S-5.8S-26S nuclear ribosomal DNA (nrDNA) has been considered one of the most important sequences for phylogenetic analysis. Here we report the analysis of nrDNA's ITS sequences as an efficient tool t...
Article
Full-text available
Even though radiocontrast nephropathy is usually the most common cause of acute renal failure (ARF) following invasive vascular interventions, cholesterol embolism should be considered in the differential diagnosis. It is very important to differentiate these 2 conditions because of the disparities between treatment modalities and renal-patient sur...
Article
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Marigold (Tagetes erecta L.) plants, also called Mexican or Aztec marigold, with symptoms of shoot proliferation, dwarfing, and reddening were observed in ornamental gardens of Van Province (Turkey). Five plants, two of them showing reddening and three symptomless plants, were sampled at the end of September 2014. Genomic DNA isolated from symptoma...
Article
Full-text available
Kabak (Cucurbita pepo L.), Türkiye'nin tüm coğrafi bölgelerinde yetiştirilen önemli bir üründür. Zucchini yellow mosaic virus (ZYMV), kabakgiller (Cucur-bitaceae) familyasının tüm türlerini etkilemekte ve yıkıcı etkisinden dolayı bü-yük ekonomik zararlara neden olmaktadır. Kabak türleri ve genotipleri, ZYMV'ne dayanıklılık için uzun süredir dünyanı...
Article
Full-text available
Pokeweed antiviral protein (PAP) is single-chain ribosome-inactivating protein (RIP) of Phytolacca americana L. (Pokeweed) that is characterized by its ability to depurinate ribosomes. In the present study, we cloned and sequenced complete gene of pokeweed antiviral protein type 1 (PAP-I) from the summer leaves of pokeweed collected from Trabzon (T...
Article
Full-text available
Two main apple growing provinces (Van and Malatya) of East Anatolia were surveyed for the presence of the Apple mosaic virus (ApMV). Dot-blot hybridization and RT-PCR tests were implemented to investigate the incidence of ApMV, testing a total of 481 samples collected from commercial apple orchards. Digoxigenin-labeled RNA probes of the ApMV were s...
Article
Full-text available
The prevalence of Potato spindle tuber pospiviroid (PSTVd) infection in randomly selected potato tubers was determined via nonisotopic dot blot hybridization and reverse transcriptase polymerase chain reaction (RT-PCR). In January and February of 2010, 168 seedling tubers of Solanum tuberosum, representing 27 cultivars, were received from different...
Article
Full-text available
Th e coat protein (CP) genes of the genomic RNA of 2 severe Turkish isolates of Zucchini yellow mosaic virus (ZYMV) from squash and muskmelon [ZYMV-Adana (Ad) and ZYMV-Ahlat (Ah), respectively] were cloned, and their complete nucleotide sequences and deduced amino acids were determined. Th e analysis revealed that both Turkish ZYMV-CP genes contain...
Article
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In this study, it is aimed to asses the association of arbuscular mycorrhizal (AM) fungi within colonised rhizosphere of Gramineae family members through a survey by using nested- polymerase chain reaction method in Van province (Turkey). From 24 agro-ecological fields, a total of 82 samples belonging to Gramineae family were tested by molecular me...
Article
Plum bark necrosis stem pitting associated virus (PBNSPaV) has occurred globally over the last two decades and is becoming one of the major agricultural issues of stone fruits. The virus was detected for the first time in plums and has been reported in the other stone fruit species. The agent, whose dissemination mode is still unknown, has been fir...
Article
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Two new cherry leaf roll virus (CLRV) isolates (Ah and Ad) were isolated and detected from traditionally-grown walnuts that showed severe apical necrosis and chlorotic spots in systemically infected Cheneopodium amaranticolor, in the eastern part of Turkey. The 404 and 405 bp long DNA fragments of the 3'-non-coding region of both isolates from PCR...
Article
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A validated RT-PCR method was used to investigate the presence of Apple scar skin viroid (ASSVd) in the commercial apple orchards of eastern Anatolia. Among three modified and simplified silica-capture based extraction methods, one was used for mass extraction to ascertain the presence of ASSVd. The test was initially performed from an ASSVd-infect...
Article
Full-text available
SUMMARY Walnut orchards of the Lake Van basin (Turkey) were surveyed from June to October 2006 to determine the incidence of viral infections. ELISA and RT-PCR were used to investigate the presence of Cherry leaf roll virus (CLRV) and Plum pox virus (PPV), testing a total of 870 samples collected from traditional seed-grown plantations. Whereas no...
Article
The specific complementary association due to hydrogen bonding of single stranded nucleic acids is referred to as annealing. Successful primer annealing is of critical importance in a polymerase chain reaction (PCR). In this study, the effect of gradually increased and decreased annealing temperatures on PCR reactions were investigated. In the anne...
Article
Field surveys were carried out to determine presence and incidence of Apricot latent virus (ApLV) and Plum bark necrosis stem pitting-associated virus (PBNSPaV) in the main stone fruit growing areas of Eastern Anatolia. RT-PCR and nested-RT-PCR techniques were used to detect ApLV and PBNSPaV, respectively. Three apricot samples out of 224 tested po...
Article
Full-text available
The presence of virus/viroid infections can go unnoticed since symptoms appear only if additional viruses are present. Detection of Plum bark necrosis stem pitting associated virus (PBNSPaV), Apricot latent virus (ApLV), Apple scar skin viroid (ASSVd), Prunus necrotic ringspot virus (PNRSV) and Potato virus Y (PVY) by reverse transcriptase polymera...
Article
The efficiency of RNA extraction for Apricot latent virus (ApLV), Plum bark necrosis stem pitting associated virus (PBNSPaV), Prunus necrotic ring spot virus (PNRSV), Potato virus Y (PVY), and Apple scar skin viroid (ASSVd) from infected hosts is of great importance for molecular diagnosis by the polymerase chain reaction (PCR). A method is describ...
Article
Full-text available
Stone fruit trees were surveyed from June to october (2003 and 2004) to determine the incidence of viroid, phytoplasma, and fungal diseases in the eastern anatolia region of turkey. Molecular hybridisation test (tissue printing and dot-blot) was used to investigate the presence of Peach latent mosaic viroid (plMVd) and Hop stunt viroid (HSVd). a to...
Article
Full-text available
In order to have a more detailed understanding of the various factors influencing a reverse transcriptase polymerase chain reaction (RT-PCR), a number of important parameters such as Mg+2, primer, enzyme concentration and others were optimized for the detection of Prunus necrotic ringspot virus (PNRSV). Using a PNRSV isolate with a pair of primers,...
Article
Full-text available
z: Prunus necrotic ringspot virus (PNRSV)'ü ile infekteli Prunus mahaleb ve Apple chlorotic leafspot virus (ACLSV)'ü ile infekteli şeftali (P. persica L.) yapraklarının farklı bölgelerinden alınan doku diskleri bu virüslerin yaprak dokusundaki dağılımlarını belirlemek amacı ile sırasıyla enzyme-linked immunosorbent assay (ELISA) ve reverse transcri...
Article
Full-text available
Leaf discs taken from different canopy and leaf sites of Prunus necrotic ringspot virus (PNRSV) infected Prunus mahaleb and Apple chlorotic leafspot virus (ACLSV) infected peach (P. persica) tree were analyzed for the determination of virus distribution in leaf tissues by enzyme-linked immunosorbent assay (ELISA) and reverse transcriptase polymeras...
Article
Full-text available
(2) Abstract: Two methods (DAS-ELISA and RT-PCR) were compared for the detection of Prunus necrotic ringspot virus (PNRSV) in woody host Prunus mahleb isolated from Malatya. Total RNA extractions were made from serially diluted fresh leaf tissue, root, bark and one year old green bark tissue using silica-based method. Purified total RNA extracts we...

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