
Mustafa Kemal SoyluBursa Uludağ Üni̇versi̇tesi̇ · Department of Reproduction and Artificial Insemination
Mustafa Kemal Soylu
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Publications (45)
This study aimed to determine the influence of different doses of β-glucan on post-thaw spermatological parameters, lipid peroxidation, and total antioxidant activity of buck semen. In the non-breeding season, semen was collected from bucks twice weekly. After then, ejaculates were pooled and divided into four equal aliquots: β-glucan concentration...
The aim of this study was to elucidate the toxicity effect of widely used penetrating cryoprotective agent dimethylsulfoxide (DMSO) on frozen drone semen and fertility. DMSO was replaced totally or partially with either dextran, polyvinylpyrrolidone (PVP), or polyethylene gylchol (PEG), which are non-penetrating cryoprotectants. The freezing extend...
In this study, it was aimed to determine the effect of destruction of lyophilized and frozen-thawed ram sperm plasma and acrosomal membrane on development of embryos produced by intracytoplasmic sperm injection (ICSI). Semen samples were divided into two groups for lyophilization (L) and freezing (F). For the removal of the plasma membrane, L and F...
The aim of this study was to determine the effects of both the removal of seminal plasma (SP) and the pre-freezing addition of seminal plasma collected during the breeding or nonbreeding season on goat sperm survival after thawing. Semen samples were pooled. One aliquot of pooled semen was used as a control group. Four aliquots were then centrifuge...
Summary: The aim of the current study was to evaluate different antioxidant-supplemented extenders for post-thaw
semen quality and incubation resilience of ram spermatozoa. Pooled semen samples were divided into four equal volumes
and each volume were diluted with two-step dilution method in control and antoxidant supplemented groups
(5mM methionin...
The aim of the current study was to evaluate the effects of different concentrations of rainbow trout seminal plasma (RTSP) (0.1%, 1% and 10%) in extenders containing either egg yolk or lecithin for use in Awassi ram semen cryopreservation. Pooled sperm were diluted in a two-step dilution method to a final concentration of 1/5 (semen/extender) in e...
The aim of this study was to evaluate the effects of lyophilized egg yolk extender on ram semen cryopreservation. Ejaculates with a thick consistency, rapid wave motion (3-5 on a 0-5 scale) and >75% initial motility were pooled. Sperm were diluted to final concentration of 1/5 (semen/extender) in lyophilized egg yolk or fresh egg yolk extenders usi...
This study investigates the effect of freezing rates on the spermatological parameters of frozen and thawed Saanen goat semen. Equilibrated semen was frozen at 4 different freezing rates from +5 degrees C to -150 degrees C (G10: 10 degrees C/min, G12: 12 degrees C/min, G15: 15 degrees C/min, and G24: 24 degrees C/min) and stored in liquid nitrogen...
The aim of the present study was to evaluate the correlation between the routine semen analyses and sperm DNA integrity, as assesed by TUNEL in fresh semen of rams in Kivircik and Awassi breeds in breeding season. Semen was collected by electro-ejaculation five times every other day. For that purpose, 50 fresh ejaculates from 6 Kivircik and 4 Awass...
The aim of the current study was to evaluate the effect of egg yolk and different soybean lecithin concentrations on the efficiency of ram semen cryopreservation and to test the fertilizing ability of frozen-thawed ram semen. Ejaculates with a thick consistency, rapid wave motion (3-5), and >70% initial motility were pooled. Pooled semen were then...
The aim of the present study was to investigate the suitability of the water test and hypo-osmotic swelling (HOS) test for
analyzing honeybee semen. In particular, the relationships between these tests of the integrity of the sperm plasma membrane
and tests of sperm motility and sperm viability were measured using SYBR-14/PI. To this end, semen wa...
The influence of different extender osmolality levels and the presence of different cryoprotectants on the post- thawed semen's characteristics and post-thawed plasma membrane integrity of ram spermatozoa were studied. Ram semen was frozen with TRIS-egg yolk based extender according to two-step dilution procedures. The final concentrations of the c...
Biventricular pacing for the treatment of congestive heart failure has been shown to improve symptoms. To pace the left ventricle, the lead is implanted in the tributaries of the coronary sinus. However, 8% to 10% of the procedures result in failure to implant the coronary sinus leads. Implantation of a biventricular pacemaker was planned in a 57-y...
The aim of the study was to evaluate the effect of the addition of 20% bull seminal plasma to semen extenders on post-thaw sperm motility, morphology, and fertility. Two Merino rams at 3-5 years of age, 43 ewes, and one Holstein bull were used. Fourteen ejaculates were collected by the use of an artificial vagina, twice a day, at 60 min interval. S...
The objective of the present study was to evaluate some semen characteristics in Saanen bucks during the transition period. A total of 60 ejaculates were obtained from 6 bucks and was subjected to routine semen analysis. In general, all studied semen parameters were similar to the other breeds. Abnormal sperm cells positively correlated with dead s...
The objective of this study was to investigate the effect of low temperature thawing and post-thaw cold shock application on sperm motility, as well as acrosomal and plasma membrane integrities of cryopreserved bull semen. Frozen semen was thawed in a water bath at 5-7°C for 30, 60 or 90 s (low thawed groups), cold shocked (at 5-7°C for 30, 60 or 9...
Objectives: We evaluated patients with ventricular tachycardia (VT) with respect to etiological factors, demographic and clinical features, in-hospital mortality and reviewed recent therapeutic approaches in high-risk patients. Study design: This retrospective study included 392 consecutive patients (111 females, 281 males; mean age 59.2 years; ran...
Magnesium is the second most common intracellular cation and plays an important role in the activities of more than 300 enzymes. Thus, magnesium is used in cardiology for both prevention and treatment of arrhythmias and ischemic heart disease. In the patients with coronary heart disease, especially during acute myocardial infarction, it has been us...
Results indicate that glucose or fructose can be used as an energy source for the Tris-citric acid extender for the short time storage of rabbit semen. According to time differences (0, 24, 48, 72 h) there were statistically significant (P<0.05) differences among motility, dead sperm cells and morphological (head, mid, acrosome) characteristics exc...
The efficiency of medroxyprogesterone acetate (MAP) and fluorogestone acetate (FGA) sponges with or without PGF 2α (cloprostenol) for synchronizing estrous in non-lactating does was investigated during the natural breeding season. Does were treated for 11 days with 60 mg MAP ( n = 38) or 40 mg FGA ( n = 32) sponges. All does also received intramusc...
The aim of the present study was to assess the relationships between sperm membrane integrity, evaluated by the hypo-osmotic swelling (HOS) and water tests, and sperm quality parameters (percentage of dead, motile and abnormal spermatozoa) in goat bucks. For that purpose, a total of 60 fresh ejaculates from 6 Saanen bucks were evaluated. The mean p...
The objective of this study was to compare the fertility levels of goats inseminated with fresh semen and buck semen diluted with three different extenders. The fertility rates of goats inseminated in spontaneous oestrus during breeding season were found 66.6% (12/18), 60.8% (28/46), 44.4% (12/27) and 48.7% (20/41) for fresh semen, Laiciphos 488-eg...
This study was performed with 18 German Shepherd female dogs whom previous cycles were recorded. Bitches were separated into three groups: two treated groups and a control one. The first group was selected from 7 bitches in early anestrous period, i.e., at the stage 108 to 124 days after the beginning of their last pro-oestrus. The second group con...
Semen was obtained from five Kivircik rams by electro-ejaculator and the seminal plasma was obtained by centrifuging the semen of a Holstein bull, which was collected by an artificial vagina, at 3000 rpm for 10 minutes. Pooled ram semen was split into three group. After the addition of extenders the final volume rates of bull seminal plasma in grou...
________________________________________________________________________________ Abstract The efficiency of medroxyprogesterone acetate (MAP) and fluorogestone acetate (FGA) sponges for synchronizing oestrus in lactating Saanen goats was investigated during the transition from non-breeding to natural breeding season. Does were treated for 11 days w...
Amiodarone is a clas III antiarrhythmic agent that is used in treatment of both supraventricular and life-threatening ventricular arrhythmias in adult and pediatric population. Although lengthening of QT interval is seen frequently during the long term use of amiodarone, it rarely causes polymorphic ventricular tachycardia (PVT) called "torsade de...
The aim of this study was to investigate the possibility of using synthetic serum substitute (SSS) instead of fetal calf serum (FCS) in maturation medium to stimulate in vitro maturation (IVM), fertilization (IVF) and subsequent development of bovine oocytes. Ovaries were obtained from a local slaughterhouse. Selected oocytes were matured in tissue...
The aim of this study was to study the presence and incidence of Mycoplasmas in the genital tract of bulls slaughtered at Bursa abattoir in Bursa province. A hundred and fifteen preputium washings and 115 epididymal swab samples of bulls from Bursa abattoir were investigated for Mycoplasma spp. Fourty two (36.52%) Mycoplasma strains were isolated f...
QT interval dispersion is an attempt to measure noninvasively the propensity of the heart to support reentrant circuits. QT dispersion increases in patients with acute myocardial infarction (MI) and a quicker restoration of blood flow in the infarct related artery decreases QT dispersion. Effects of collateral blood flow on QT dispersion and the oc...
In the present study, ten ejaculates were obtained from each of seven German shepherd dogs. Only the sperm-rich fractions of ejaculates from each dog once a week with a mean interval of 60 min, were used during five weeks. Semen quality was examined in total of 70 semen samples. After this examination, semen samples were centrifuged (5000 g × 10 mi...
The objective of this study was to evaluate effect of different thawing procedure to Hypo-Osmotic Swelling Test (HOST), motility and morphology of bull semen. Straw (0.25 ml) frozen semen from 4 Holstein bulls was used in this study. Post-thaw motility, morphology and hypo-osmotic swelling test (HOST) were carried out at 37°C for 30 sec, 50°C for 1...
The Brugada syndrome is a malignant primary electrical disease of the heart caused by a defect in an ion channel gene, resulting in abnormal electrophysiologic activity in the right ventricle and characterized by(1) a phenotypic ST-segment elevation in the right-sided precordial leads often accompanied by apparent conduction block in the right vent...
A seroepidemiological study was conducted to assess the seroprevalence of hepatitis B surface antigen (HBs Ag) and antibodies to hepatitis C virus (Anti-HCV) in 14,196 patients hospitalized in our cardiology clinics. Threehundred and fifty five (355) patients (2.43%) were found to be positive for HBs Ag, a hundred and sixty six (166) patients (1.09...
Radiofrequency (RF) catheter ablation of atriofascicular accessory pathways (AP) characterized by decremental antegrade conduction property and result in antidromic tachycardia with left bundle branch morphology is a current preferred theurapetic approach. Two patients, who were twenty-two and thirty-six years old, with atriofascicular AP and drug-...
In this study, we aimed to determine the levels of some biochemical parameters in the seminal plasma of German Shepherd dogs. Seven German Shepherd dogs were used as materials. A total of 35 ejaculates, five from each dog, were collected. The seminal plasma of each ejaculate was separated by centrifugation at 5000 rpm for 10 min. Biochemical analys...
The aim of this study is to investigate the some spermatological characteristics of New Zealand White
rabbit bucks. Ten New Zealand White males, aged 12-18 months, were used as materials. Five ejaculates were collected from each buck on every other day by means of artificial vagina. The averages of ejaculate volume, pH, spermatozoa concentration, m...
The aim of this study was investigated of freezability of ram semen during the transition from nonbreeding
to the natural breeding and breeding season. In this study, collected ram semen was diluted Tris-egg yolk
extender in 1:1 ratio finally including 5% glycerol and frozen in 0.25 ml straws both during the transition from nonbreeding to the natur...
This study was designed to assess effectiveness of different staining methods, Eosin-nigrosin (EN),
Giemsa and double staining method Eosin-nigrosin combined with Giemsa (EN-G), to detect dead spermatozoa,
defected acrosome rates (AB), Other Morphological Defect (DMB) and Total Morphological Defect (TMB) in fresh Saanen semen. The percentages of sp...
The objective of this study was to compare the developmental ability of embryos in 4 different media, CR 1aa, modified CR2, BCEM-g and TCM-199. Bovine oocytes were matured and then fertilized in vitro. A total of 915 oocytes were used for fertilization, 681 (74%) of which cleaved. The 2-cell embryos were divided into 4 groups, and cultured in 1 of...
Carotid artery atherosclerosis is a strong predictor of stroke and represents a potential source of cerebral emboli. The aim of this study was to investigate whether an association exists between mitral annular calcification and carotid atheroma. In addition, the characteristics of carotid atheromas were compared between patients with and without m...
It has been shown that QT dispersion (QTD) increases during episodes of myocardial ischemia or infarction. However, no extensive data on the relation between the diseased coronary artery or the localization of stenosis and the QTD are available. The aim of the study was to examine the relation between QTD and diseased coronary artery and lesion loc...
In the present study, a total of 45 cervical mucus samples obtained from 30 fertil and 15 repeat breeder cowe was studied. Fifteen of 30 fertil cows were injected wiyh prostaglandin F2 alfa.
In the first step, semen samples were diluted with Tris+Citric acid+Fructose+Egg yolk and Sadium citrate+Egg yolk diluents and were frozen in liquid nitrogen vapour. In the second step, 120 Merino ewes were synchronised with sponges, which contained flourogestone acetate, for 14 days. PMSG was injected into the ewes on the withdrawal day. Ewes were...