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1 Research Item
PhD student at the University of Munich (LMU). (Current research interests: Fluid-Flow and Orthodontic tooth movement) (Methods and Techniques: Mostly ELISA and PCR; cell culturing) (Working on now: Mechanobiology of orthodontic tooth movement: Fluid-Flow Shear Stress)
Oropharyngeal candidiasis is a serious clinical dilemma within the immunosuppressed and elderly population, commonly caused by Candida albicans species. The ability of C. albicans to infect the epithelial cells is mediated by the dimorphic switching to virulent hyphae form while expressing different virulence patterns to facilitates cell invasion....
I have noticed that there are single microscopic slide/slip chambers (Cytodyne, Flexflow, IBIDI) and many studies have used these chambers. I wondered how it is possible to have more robust data by using a single fluid flow chamber (1 replicate) and a control?
I have noticed while reading different publications that some have used collagen to coat slide/chamber surface in fluid-flow cell experiments, while others used fibronectin. Does coating rely on the type of cells that I am going to use? Example: collagen coating for bone cells, fibronectin coating for endothelial cells?
How long does fluid flow within bone lacunae last when applying a constant force?
Is it going to last for a few seconds till fluid flow equilibrium occurs?
During jogging, walking and jumping (force is not constant) we usually have an Oscillatory fluid flow within bone lacunar canals. Will applying a constant tension force going to result in Laminar fluid flow within the bone lacunar-canalicular system?
As you know peristaltic pump has a Constant fluid flow direction but Changing fluid flow rate. I was wondering if it is possible to produce a Constant (Steady) fluid flow rate using peristaltic pump.
I am planning a fluid dynamic experiment and wonder what tubing material is biocompatible for cell culturing experiment. Also wonder if there is a tubing material that can be sterilize.
I am building a fluid-flow chamber, However, how can I calculate the amount of culture media needed for the flow experiment? does this depend on the size of the flow chamber? is there any method to calculate that?
I would like to know how to coat a specific area (for example: the center of the slide only) with collagen typ1? Should I coat the whole slide then use a blade to scrape the undesired coated area?
I have been searching for the equation of calculating the pressure inside a rectangular pipe using volumetric flow rate but all I am finding is the Hagen–Poiseuille equation that calculates the pressure inside a cylindrical pipe: Flow rate=πr4(P−P0)/8ηl. Any idea?
I am wondering if FAK +\+ mice osteoblasts does behave similar as Wild type mice osteoblasts “in vitro” when both of them are used as a control in a given experiment?
I was wondering where to find the viscosity of cell culturing media under 37C, As I am planning to do a microfluidics experiment using DMEM (high and low glucose) and KSFM at 37C temperature?
If we have a poroelastic square and we tried to stretch it in all dimension, does that create a low pressure inside the square as the pore size increase, due to the stretching of the square?
-ELISA is used to determine protein release in the supernatant bathing medium, after a given period of time.
-qRT-PCR is used to determine gene expression at real time after a given period of time.
-If we have an experiment that aims to determine the effect of drug X on the gene expression and protein release of IL-6 in a given cell type, after 3,6 and 9 hours.
As protein accumulate in the bathing media over time:-
3h: 10 mg/dl
6h: (3h)+ 6 mg/dl =16 mg/dl
9h: (6h) + 4 mg/dl = 20 mg/dl
3h: 10 fold change
6h: 6 fold change
9h: 4 fold change
How would the data from qRT-PCR and ELISA be compared when we have at 9h down regulation of gene expression of IL-6 BUT “a false positive“ increase in protein release due to accumulation over time?
1- Collect and analyse data from in vitro studies that investigated the effect of fluid shear stress (FSS) on cells representing cells of the periodontium, such as Mesenchymal stem cells, periodontal ligament cells, osteocytes and osteoblasts of murine and human origin. 1- Construction of FSS apparatus (in vitro model) 2- Determine the effect of FSS on cells of the Periodontium.