Mustafa Bahceci

Bahceci Hospital and IVF Center, Pristina · IVF

Purpose Our study aimed to determine the possible factors that might impact the probability of obtaining a euploid blastocyst following intracytoplasmic sperm injection (ICSI) and preimplantation genetic testing for aneuploidy (PGT-A) procedures in idiopathic recurrent pregnancy loss (RPL) patients. Methods This single-center retrospective cohort...

Objective: To investigate whether endometrial thickness (EMT) affects birth weight and maternal serum pregnancy-associated plasma protein-A (PAPP-A) level in singleton pregnancies following frozen-thawed blastocyst transfer (FET). Methods: This retrospective study included women who underwent first-trimester combined screening for aneuploidy aft...

Uterus transplantation is a novel approach in women whose uterus is absent or severely abnormal. However, it is still an experimental procedure that poses risks to both the mother and baby. To date, 32 live births after uterus transplantation have been reported in peer-reviewed journals with several maternal, fetal, and neonatal complications. The...

Objective To compare the ongoing pregnancy rates (OPRs) for subcutaneous progesterone (SC-P) to intramuscular progesterone (IM-P) in hormone replacement therapy used frozen embryo transfer (FET) cycles. Design Prospective non-randomized cohort study Setting Private fertility clinic Patients The study enrolled 224 patients scheduled for HRT-FET c...

Study question Is there a ceiling effect of high serum progesterone levels on day of embryo transfer for pregnancy outcomes in patients undergoing artificial frozen-thawed blastocyst transfer? Summary answer There is no ceiling effect of high serum progesterone levels on day of embryo transfer(>40 ng/mL)for pregnancy outcomes in patients undergoin...

Assisted reproduction technology has two significant problems: low success rates and multiple pregnancies. Because of these problems, the priority in IVF clinics is to develop a potential diagnostic test that can be used to select the embryos with the ultimate developmental competence. Aneuploidy screening as embryo selection criteria will ensure t...

Amaç: Bu çalışmada kendi gamet hücreleri kullanılarak Yardımcı Üreme Teknikleri (YÜT) tedavisi gören çiftlerde, inseminasyon için kullanılan In Vitro Fertilizasyon (IVF) veya Intra- Sitoplazmik Sperm Enjeksiyonu (ICSI) yönteminin embriyo morfokinetik gelişim zamanlarında bir değişiklik yapıp yapmadığının araştırılması amaçlanmıştır. Gereçler ve Yön...

Background Previous evidence indicates associations between the female reproductive tract microbiome composition and reproductive outcome in infertile patients undergoing assisted reproduction. We aimed to determine whether the endometrial microbiota composition is associated with reproductive outcomes of live birth, biochemical pregnancy, clinical...

Objective: To describe surgical procedures, previous failed pregnancies, methods for overcoming pregnancy failure and, most importantly, birth of a healthy infant, in a uterus transplantation from a deceased donor. Background: Majority of uterus transplants have involved live donors, but several advantages make deceased donor transplantation a p...

Objective: the aim of the study was to determine whether endometrioma aspiration and alcohol sclerotherapy performed prior to controlled ovarian stimulation has any beneficial effects on ICSI outcomes in patients with endometriomas. Methods: The cycle characteristics and pregnancy outcomes of 103 patients with endometriomas who underwent ethanol sc...

OVULASYON TETİKLEME GÜNÜ YÜKSEK PROGESTERON DEĞERİNİN, GELİŞEN EMBRİYO ÜZERİNE ETKİSİ; KROMOZOMAL PERSPEKTİF ÖZET: Amaç :Kontrollü ovaryan hiperstimulasyon sikluslarında, ovulasyon tetikleme günü yüksek progesteron düzeylerinin, gelişen embriyonun, kromozom yapısı üzerine etkilerini araştırmak. Gereçler ve Yöntem: Bu retrospektif çalışmada, Bahçeci...

Study question What is the effect of varying diagnostic thresholds on the accuracy of Next Generation Sequencing (NGS)-based preimplantation genetic testing for aneuploidies (PGT-A)? Summary answer When single trophectoderm biopsies are tested, the employment of 80% upper threshold increases mosaic calls and false negative aneuploidy results compa...

Study question Does SC-P provide similar ongoing pregnancy rates (OPRs) as intramuscular progesterone(IM-P) in hormone replacement therapy (HRT)-FET cycles and do serum progesterone (P) levels on FET day effect on pregnancy outcome? Summary answer: SC-P administration had similar OPR compared to IM-P in HRT-FET cycles. In SC-P group embryo transfer...

Study question Does the outcome of the first euploid frozen-thawed blastocyst embryo transfer affect the subsequent euploid FBT originating from the same cohort of oocytes? Summary answer The clinical pregnancy rate and ongoing pregnancy rate of the subsequent FBT are higher if a clinical pregnancy was attained in the first euploid FBT. What is k...

Study question: What is the effect of varying diagnostic thresholds on the accuracy of Next Generation Sequencing (NGS)-based preimplantation genetic testing for aneuploidies (PGT-A)? Summary answer: When single trophectoderm biopsies are tested, the employment of 80% upper threshold increases mosaic calls and false negative aneuploidy results comp...

ABSTRACT Research question: Which parameters affect the likelihood of miscarriage after single euploid frozen–thawed blastocyst transfer (FBT)? Design: In this retrospective study, clinical and laboratory data from 1051 single euploid FBTs were evaluated. Exclusion criteria were endocrine or systemic pathologies, uterine anomalies or pathologies,...

Research question: Which parameters affect the likelihood of miscarriage after single euploid frozen-thawed blastocyst transfer (FBT)? Design: This is a retrospective study. Clinical and laboratory data from 1051 single euploid FBTs were evaluated. Exclusion criteria were endocrine or systemic pathologies, uterine anomalies or pathologies, unilater...

Background: Previous evidence indicates associations between the female reproductive tract microbiome composition and reproductive outcome in infertile patients undergoing assisted reproduction. We aimed to determine whether the endometrial microbiota composition is associated with reproductive outcomes of live birth, biochemical pregnancy, clinica...

Purpose: To investigate whether there is any detrimental effect of progesterone elevation (PE) on the day of oocyte maturation induction on embryological development potentials. Methods: This retrospective single-center cohort study included a total of 1485 individual intracytoplasmic sperm injection (ICSI) cycles between January 2014 and Decemb...

Purpose To evaluate the factors that affect the incidence of euploid balanced embryos and interchromosomal effect (ICE) in carriers of different structural rearrangements. Methods This retrospective study includes 95 couples with reciprocal translocations (RecT) and 36 couples with Robertsonian translocations (RobT) undergoing Preimplantation Gene...

Research Question : Does an association exist between ovarian reserve, ovarian response, and embryonic euploidy in female patients under age 35? Design : A retrospective analysis of intracytoplasmic sperm injection (ICSI) and preimplantation genetic testing for aneuploidy (PGT-A) cycles among patients enrolled in Bahceci Fulya IVF Center between J...

Objective: Cryopreservation of embryos for future transfer attempts has noticeably increased in the last decade, especially due to the technological developments in in vitro fertlization (IVF) laboratories. In parallel, different progesterone (P) replacement regimens preceding artificially prepared frozen embryo transfer (AC-FET) attempts, especia...

Infertility is a global health problem, affecting an estimated 50 million couples worldwide. It is generally accepted that in 30% of infertile couples, the problem is confined solely to issues related with sperm production, while in 50% of these cases, the problem is a combination of both female and male factors. Since 1992, intracytoplasmic sperm...

mosaic classification in blastocysts-stage primplantation genetic testing

Aim: To evaluate the effect of trigger day progesterone (P) levels on live birth in freeze-all cycles. Material and methods: Retrospective analysis of 1034 freeze-all female patients aged <38 years with single blastocyst transfers. Patients with (n = 268) or without (n = 766) preimplantation genetic test for aneuploidy (PGT-A) arm were further cate...

Background Is freeze-all strategy effective in terms of cumulative live birth rates (CLBRs) in all patients? Methods This retrospective single-center study analyzed the CLBRs of 2523 patients undergoing fresh or electively frozen blastocyst transfer cycles. In 1047, cycles, the fresh embryo transfer (ET) strategy was applied for the 1st ET, wherea...

Purpose: The existing literature is scarce regarding outcomes of fresh vs frozen embryo blastocyst transfers in women with endometrioma. The goal of the study was to compare outcomes between fresh and frozen blastocyst transfers (fresh-BT vs. frozen-BT) in endometrioma-affected women. Materials and methods: This study included 315 endometrioma-affe...

Research question Does the use of preimplantation genetic testing for aneuploidy (PGT-A) improve outcomes in couples with severe male factor infertility (SMFI)? Design : This retrospective cohort study included SMFI cases that underwent blastocyst transfer with/without PGT-A. Inclusion criteria were SMFI (azoospermia and sperm count<1 million/mL),...

Background The recent identification of embryo cell-free DNA in the spent blastocyst media opened a new era of possibilities for non-invasive embryo aneuploidy testing in assisted reproductive technologies. Yet, previous studies assessing limited numbers of embryos reported variable concordance between embryo cell-free DNA and trophectoderm biopsie...

Despite next-generation sequencing, which now allows for the accurate detection of segmental aneuploidies from in vitro fertilization embryo biopsies, the origin and characteristics of these aneuploidies are still relatively unknown. Using a multifocal biopsy approach (four trophectoderms [TEs] and one inner cell mass [ICM] analyzed per blastocyst;...

Background To assess the predictive value of patient characteristics, controlled ovarian stimulation and embryological parameters on the live birth outcome of single euploid frozen-warmed blastocyst transfer (FBT). Methods This was a retrospective cohort study including 707 single FBTs after preimplantation genetic testing for aneuploidy (PGT-A) t...

Background: Endometrial preparation with hormone replacement therapy (HRT) is the preferred regimen for clinicians due to the opportunity to schedule the day of embryo transfer and for patients due to the requirement of fewer visits for frozen-warmed embryo transfers (FET). The increasing number of FETs raises the question of the serum P levels re...

Research question: Can serum oestradiol concentrations on the day of progesterone initiation predict live birth rates in single, autologous vitrified-warmed blastocyst transfers following artificial endometrial preparation? Design: This retrospective study included the first transfers of 468 patients with unexplained or tubal factor infertility...

Introduction The introduction of highly sensitive Next Generation Sequencing (NGS) platforms for PGT-A applications concurred with an increased detection of segmental aneuploidies. Recent studies focused on understanding whether segmental aneuploidies derive from mitotic or meiotic events, however, definitive data are still lacking. To further cont...

Purpose To compare outcomes between daily intramuscular progesterone (IMP) and daily vaginal progesterone (VP) gel plus weekly intramuscular hydroxyprogesterone caproate (IMHPC) for luteal phase support (LPS) in single, autologous euploid frozen-thawed blastocyst transfers (FBTs) following artificial endometrial preparation (EP). Methods The retro...

Abstract Background There is lack of population level data on prevalence and distribution of common benign women’s health conditions such as endometriosis, uterine fibroids, polycystic ovary syndrome from the Eastern Mediterranean region despite their significant consequences on quality of life. In particular, there is complete absence of any healt...

Introduction MtDNA copy number analysis remains controversial as a biomarker for embryo implantation and viability assessments with different authors reporting conflicting results. Initial reports (Fragouli et al., 2015, Diez-Juan et al., 2015) indicated that embryos with unusually high levels of mtDNA did not implant and those that did have a mean...

Introduction Comprehensive chromosomal screening at the blastocyst stage by aCGH/NGS analysis has become increasingly common approach in order to obtain high implantation rates in ART. Although utilization of chromosomally screened blastocyst-stage embryos is known to provide superior clinical outcome, not all blastocysts display the same pace of e...

Introduction Aneuploidy is generally accepted as one of the main causes of IVF/ICSI treatment failures worldwide. For this reason, increasing number of clinics & infertility specialists seek and promote preimplantation genetic testing for aneuploidy (PGT-A) to improve the clinical outcome for indications advanced maternal age (AMA), repeated implan...

Introduction PGT-A for translocations has been a valid approach in order to detect chromosomally normal/balanced embryos. While earlier studies utilized PGT-A for only rearranged chromosomes, comprehensive chromosomal analysis by array comparative genomic hybridization (aCGH) is nowadays the gold standard in PGT-A for translocation cases, allowing...

The aim of this study was to evaluate the possible effects of endometriosis on early embryo development, by comparing the morphokinetic development of embryos obtained from women with clinically confirmed endometriosis with the ones obtained from tubal factor infertility cases. A total of 82 cycles/patients including 53 cycles with endometriosis an...

Low sperm quality has negative effects on fertilisation and embryo development. The males with azoospermia apply for testicular sperm extraction (TESE) or microsurgical epididymal sperm aspiration (MESA) in order to retrieve sperm. To date, there have not been any reports investigating morphokinetic parameters of pre‐implantation embryos using test...

Practical Problems in Assisted Conception - edited by Ying Cheong September 2018

Cambridge Core - Obstetrics and Gynecology, Reproductive Medicine - - edited by Ying Cheong

Study question: Ovarian hyper-stimulation can rarely occur spontaneously in patients not undergoing ovulation induction therapies. Could we determine underlying factors via high throughput sequencing? Summary answer : By using whole exome sequencing, a homozygous autosomal mutation has been identified in two affected sisters in the family. What is...

Study question: Endometriosis has long been suspected of familial tendencies. Our goal is to identify genes involved in the pathogenesis of ovarian endometriosis by using genomic approach. Summary answer : Whole exome sequencing (WES) on a large consanguineous Turkish family revealed a possible causative gene mutation segregating with ovarian endom...

The aim of this study was to evaluate the feasibility and efficiency of using a sucrose gradient-based warming protocol as a universal warming approach on human cleavage stage embryos. Between January 2013 and November 2014, a total of 118 warming cycles were performed on 705 embryos which had previously been cryopreserved/thawed by slow freezing p...

PurposeThe purpose of this study was to identify mutations that cause non-syndromic male infertility using whole exome sequencing of family cases. Methods We recruited a consanguineous Turkish family comprising nine siblings with male triplets; two of the triplets were infertile as well as one younger infertile brother. Whole exome sequencing (WES)...

An early stage of preimplantation, mammalian embryo development is a dynamic process. Utilization of static observations for embryo development assessment is unable to give us all the information regarding embryo kinetics and substantial events. However, Time Lapse Morphokinetic (TLM) analysis provides the means to continuously observe, monitor, re...

Objective: Carriers of reciprocal and Robertsonian translocations have a higher risk of experiencing infertility and repeated miscarriages. It is well established that with advancing maternal age, the risk of aneuploidies in embryos increases. In this study, the chance of developing balanced embryos in translocation carriers with advanced maternal...

Objective To compare the effects of fresh embryo transfers (ET) and elective frozen/thawed embryo transfers (eFET) on implantation, clinical pregnancy, and live birth rates in poor ovarian responders, as defined by the Bologna criteria. Materials and Methods All electronic databases of embryo transfers between January 2011 and January 2014 were re...

Infertility is a global healthcare problem and despite long years of assisted reproductive activities, a significant number of cases remain idiopathic. Our currently restricted understanding of basic mechanisms driving human gametogenesis severely limits the improvement of clinical care for infertile patients. Using exome sequencing, we identified...

Study question: Our currently restricted understanding of basic mechanisms driving human gametogenesis severely limits the improvement of proper clini-cal care of infertile patients. The goal of our team is to identify genes through whole exome sequencing that, when mutated, cause a male infertility phenotype that adversely affects gametes producti...

Study question: Our currently restricted understanding of basic mechanisms driving human gametogenesis severely limits the improvement of proper clini-cal care of infertile patients. The goal of our team is to identify genes through whole exome sequencing that, when mutated, cause a male infertility phenotype that adversely affects gametes producti...

Summary This study aimed to investigate the optimum number of embryos to be biopsied in order to increase the likelihood of obtaining a balanced/normal embryo following preimplantation genetic diagnosis (PGD) by fluorescence in situ hybridisation (FISH) for translocation carriers. Patients with low number of fertilised oocytes (≤5) or low number of...

Summary The aim of this study was to analyse the possible occurrence of the interchromosomal effect (ICE) in human preimplantation embryos obtained from Robertsonian and reciprocal translocation carriers focusing on ones with chromosome 10 rearrangements who were undergoing preimplantation genetic diagnosis (PGD) and to investigate whether offering...