• Home
  • Mushtak T. S. Al-Ouqaili
Mushtak T. S. Al-Ouqaili

Mushtak T. S. Al-Ouqaili
University of Anbar, College of Medicine · Department of Medical Microbiology

B.Sc., M.Sc., Ph.D. Molecular biology (Full Professor)
My recent research work is concentrating on the molecular cytogenetic especially what' regarding chromosomal aberrations

About

162
Publications
328,760
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
358
Citations
Citations since 2017
127 Research Items
337 Citations
2017201820192020202120222023020406080100
2017201820192020202120222023020406080100
2017201820192020202120222023020406080100
2017201820192020202120222023020406080100
Introduction
Dear all, The general field of my study is medical microbiology. Special works are focusing on problematic microorganisms and certain infectious disease and how to use molecular tools in the genetic screening, diagnosis and follow up of treatment. In addition to mechanisms of antimicrobial resistance. For more details, you can visit my web page (https://www.uoanbar.edu.iq/staff-page.php?ID=1204) at the following link of University of Anbar (https://www.uoanbar.edu.iq).
Additional affiliations
July 2017 - present
University of Anbar
Position
  • Vice-Chancellor for scientific affairs
January 2015 - present
University of Anbar
Position
  • Vice-Chancellor for Scientific affairs
Description
  • I have an experience in medical microbiology field, especially in antibiotic resistance, molecular diagnosis of infectious disease, molecular mechanism of drug resistance and most medically important phenomenon, microbial biofilm
Education
January 1990 - January 2007
University of Anbar
Field of study
  • Molecular Microbiology

Publications

Publications (162)
Preprint
Full-text available
Objective: This study aims to evaluate the occurrence of Y chromosome azoospermia factor (AZF) microdeletions and assess immune infertility incidence and whether there is a correlation between them. Materials and Methods: The sample included 75 infertile men who were referred to private clinics in Ramadi city as a test group and 25 healthy fertile...
Article
Full-text available
When mutation is inactivated in the follicle-stimulating hormone receptor (FSHR) gene of patients with amenorrhea, the receptor’s functionality is abolished by completely blocking it, altering the ligand–receptor complex or altering the essential hormone signal transduction. This study aims to detect the frequency and pattern of chromosomal abno...
Article
Full-text available
Objective: The aim of this paper was to assess the prevalence and association between H. pylori infection and alcohol, or cigarette use among Iraqi patients. Methods: 115 individuals needed upper gastrointestinal endoscopies in total. Reverse transcription polymerase chain reaction (RT-PCR), urea breath test, rapid urease test, CagA-IgG, and cult...
Thesis
Full-text available
Clarithromycin is the cornerstone of Helicobacter pylori (H. pylori) treatment until the emergence of clarithromycin resistant strains in high ratio. The study aimed to detect the presence of H. pylori in study patients using non�invasive test which include the conventional techniques and advanced molecular techniques using gastric tissue during...
Preprint
Full-text available
Background: Amenorrhea is a rare reproductive medical condition defined by the absence of menstruation during puberty or later life. This study aims to establish the frequency and pattern of chromosomal abnormalities (CA) in both primary amenorrhea (PA) and secondary amenorrhea (SA), and further to detect the genetic changes in exon 10 at nucleotid...
Article
Full-text available
Background: Antibiotic resistance is currently the most serious global threat to the effective treatment of bacterial infections. Antibiotic resistance has been established to adversely affect both clinical and therapeutic outcomes, with consequences ranging from treatment failures and the need for expensive and safer alternative drugs to the cost...
Article
Full-text available
Purpose: This study aimed to evaluate the presence of CRISPR-Cas system genes and their possible association with antibiotic resistance patterns of Enterococcus faecalis and Enterococcus faecium species isolated from hospital wastewater (HWW) samples of several hospitals. Methods: HWW samples (200 mL) were collected from wastewater discharged fr...
Article
Full-text available
Background Parental chromosomal aberrations are important causes of recurrent pregnancy loss (RPL). Some immunological factors such as antiphospholipid antibodies and interleukin-6 (IL-6) also contribute to this complication. The aim of this study was to determine the frequency of chromosomal abnormalities and to evaluate some of the immunological...
Article
Full-text available
Background: This study aimed to analyze the incidence of azoospermia factor a (AZFa) microdeletions in the Y chromosome and their association with male infertility in a population with azoospermia and severe oligozoospermia from Iraq. Methods: A total of 75 infertile Iraqi males and 25 healthy controls were included in this study. The semen analys...
Preprint
Full-text available
Peptic ulcer disease, chronic gastritis, and stomach cancer are all caused by H. pylori. The most notable drug for the treatment is the antibiotic clarithromycin, which is currently the drug of choice. H. pylori clarithromycin resistance has been associated with point mutations in 23srRNA, the most prominent of which are A2143 and A2144G. In H. pyl...
Article
Full-text available
Background and objectives: Peptic ulcer disease, chronic gastritis, and stomach cancer are all caused by H. pylori. The most notable drug for the treatment is the antibiotic clarithromycin, which is currently the drug of choice. H. pylori clarithromycin resistance has been associated with point mutations in 23srRNA, the most prominent of which are...
Article
Full-text available
There is still no agreement on the gold standard technique for diagnosing of H . Pylori in Iraq, as well as a paucity of data on the validity of different diagnostic techniques. This study aimed to investigate the prevalence of this bacterium with different methods and compare them to the quantitative polymerase chain reaction (qPCR) as a golden st...
Book
Full-text available
The overview on the Covid 19 vaccines and strains including the mode of action of each one and details for the updating on the vaccines type.
Article
Full-text available
Background and objective: As a major clinical concern worldwide, the occurrence of extended spectrum β-lactamases (ESBLs) has been increasingly reported in Pseudomonas aeruginosa. The goal of the study for the detection the occurrence of β-lactamase bla-OXA specifically, the OXA II gene and III in study isolates and its role in the resistance patte...
Article
Background: The major therapeutic failure in clinical settings is due to problematic biofilm-producing bacteria like Pseudomonas aeruginosa. This study aims to investigate the effect of dual-species biofilms, monosaccharide, and D-amino acids on pseudomonal biofilm. Methodology: A total of 130 patients with catheter-associated urinary tract and wou...
Preprint
Full-text available
As a major clinical concern worldwide, the occurrence of extended-spectrum β-lactamases (ESBLs) has been increasingly reported in Pseudomonas aeruginosa. The goal of the study for the detection of the occurrence of β-lactamase bla-OXA specifically, the OXA II gene and III in study isolates and its role in the resistance pattern.
Preprint
Full-text available
Male infertility may be caused by anatomical or genetic defects, systemic or neurological disorders, infections, injury, gonadotropins, and anti-sperm antibody production. This study aims to detect genetic defects in Y chromosome AZF microdeletion prevalence, immune infertility incidence, and the relationship between them.
Article
Full-text available
Defined infertility as incapability of a married couple tᴏ have children for the one-year ᴏf unprotected intercourse. Y chromosomal microdeletions are the second greatest common genetic reason of men sterility. This research aims to find the prevalence of AZF Y chromosome microdeletions in azoospermic and severe oligospermia patients. Further, to e...
Article
Full-text available
Background: Pseudomonas aeruginosa produced the maximum amount of pyocyanin pigment as a secondary metabolite as a virulence factor that responsible for increase pathogenesis. The study aims to improve our understanding of the emergence of pseudomonal biofilm formation by pyocyanin production. The correlation between minimal inhibitory concentratio...
Preprint
Full-text available
Pseudomonas biofilms cause therapeutic failures in many clinical infections. This study investigated the combined actions of the volatile oil from Eucalyptus camaldulensis and antibiotics (ciprofloxacin and gentamicin) against planktonic and biofilm populations of Pseudomonas aeruginosa. Further, the quorum sensing genes of P. aeruginosa, grown as...
Article
Full-text available
The SEN virus (SENV) has been linked to transfusion-associated non-A-E hepatitis; how- ever, information regarding SENV infections in patients with thalassemia, particularly in those with hepatitis virus co-infections, remains limited. This study investigated the fre- quency of SENV (genotypes D and H) infections in Iraqi patients with thalassemic...
Article
Full-text available
Pseudomonas biofilms cause therapeutic failures in many clinical infections. This study investigated the combined actions of the volatile oil from Eucalyptus camaldulensis and antibiotics (ciprofloxacin and gentamicin) against planktonic and biofilm populations of Pseudomonas aeruginosa. Further, the quorum sensing genes of P. aeruginosa, grown as...
Preprint
Full-text available
Pseudomonas biofilms cause therapeutic failures in many clinical infections. This study investigated the combined actions of the volatile oil from Eucalyptus camaldulensis and antibiotics (ciprofloxacin and gentamicin) against planktonic and biofilm populations of Pseudomonas aeruginosa. Further, the quorum sensing genes of P. aeruginosa, grown as...
Article
Full-text available
Background/Purpose: It well recognizes that hepatitis B infections are one of the most medically important problematic infections in the world. We compared the molecular, biochemical, and other parameters of two study groups of hepatitis B patients depending on the result of hepatitis B e antigen (HBeAg). Methodology: The serological indicators of...
Article
Full-text available
Background: Pseudomonas aeruginosa produced the maximum amount of pyocyanin pigment as a secondary metabolite as a virulence factor that responsible for increase pathogenesis. The study aims to improve our understanding of the emergence of pseudomonal biofilm formation by pyocyanin production. The correlation between minimal inhibitory concentratio...
Article
Full-text available
Objectives: To evaluate the potential probiotic properties of Lactobacillus strains isolated from feces of infants and also to determine their antimicrobial activity against some enteropathogenic bacteria. Methods: The Fecal samples were prepared from 120 infants aged less than 24 months. In total, 105 Lactobacillus strains were identified by pheno...
Article
Full-text available
Objectives To evaluate the potential probiotic properties ofLactobacillus strains isolated from feces of infants and also to determine their antimicrobial activity against some enteropathogenic bacteria. Methods The Fecal samples were prepared from 120 infants aged less than 24 months. In total,105 Lactobacillus strains were identified by phenotyp...
Data
Re-joining to American Society of Microbiology as clinical laboratory scientist for 2020.
Data
Partial sequence of the blaVEB genes from the Iraqi (A) Pseudomonas aeruginosa isolate (compared with GenBank isolate ID: KT283238.1), (B) Klebsiella pneumoniae isolate (compared with GenBank isolate ID: MF417536.1), and (C) Escherichia coli isolate (compared with GenBank isolate ID: NG055500.1).
Data
VEB gene polymorphisms present in the Klebsiella pneumonia, Pseudomonas aeruginosa, and Escherichia coli isolates relative to the global standard gene. The sequencing and BLAST analysis of the blaVEB genes isolated from the bacteria in this study showed the types of polymorphisms evident from the global standard genes.
Article
Full-text available
Objective Multi-drug resistance Gram-negative bacteria possessing Extended-Spectrum β-Lactamase (ESBL) genes are of concern because of their resistance to third-generation cephalosporins. This study aims to investigate the molecular basis of resistance to modern β-lactams by ESBLs encoded by the blaVEB gene and the gene’s role in resistance. Also,...
Thesis
Full-text available
Mycobacterium tuberculosis is the etiologic agent of Tuberculosis which affects 1.8 billion people/year which is equal to one-third of the entire world population , It is known that tuberculosis (TB) is one of the most threated disease worldwide . Its health important exacerbated by emerging of drug resistance such as development of MDR ( Multi Dru...
Preprint
Full-text available
Multi-drug resistance Gram-negative bacteria possessing Extended-Spectrum β-Lactamase (ESBL) genes are of concern because of their resistance to third-generation cephalosporins. This study aims to investigate the molecular basis of resistance to modern β-lactams by ESBLs encoded by the blaVEB gene and the gene’s role in resistance. Also, gene seque...
Preprint
Full-text available
Hepatitis B virus (HBV) infection is a major global health problem. This study aims to detect selected biochemical and hematological parameters in both HBeAg positive and negative patients suffered from chronic hepatitis B.
Research
Full-text available
A total of 50 fertile human hydatid cases {33(66%) females and (34%) males}, obtained from Al-Ramadi public Hospital during the period from December 2003 to July 2004 were examined to study any bacterial infections. The specimens were obtained from hydatid fluid and then cultured on appropriate culture media to distinguish some species of bacteria...
Preprint
Full-text available
The SEN virus (SENV) has been linked to transfusion-associated non-A-E hepatitis; however, information regarding SENV infections in patients with thalassemia, particularly in those with hepatitis virus co-infections, remains limited. This study investigated the frequency of SENV (genotypes D and H) infections in Iraqi patients with thalassemia who...
Data
SEN virus strain SEN V-D 17 ORF1 protein gene, partial cds GenBank: MK497794.1 LOCUS MK497794 150 bp DNA linear VRL 20-MAY-2019 Sequencing Technology:- Sanger dideoxy sequencing
Data
SEN virus strain SEN V-D 8 ORF1 protein gene, partial cds GenBank: MK497801.1 LOCUS MK497801 147 bp DNA linear VRL 20-MAY-2019 Sequencing Technology:- Sanger dideoxy sequencing
Preprint
Full-text available
The SEN virus (SENV) has been linked to transfusion-associated non-A-E hepatitis; however, information regarding SENV infections in patients with thalassemia, particularly in those with hepatitis virus co-infections, remains limited. We determined the frequency of SENV-H infections in patients with thalassemia who were and were not infected with he...
Preprint
Full-text available
Objective: Gram-negative bacteria possessing extended-spectrum beta-lactamase (ESBL) genes are of concern to the medical community because of their resistance to third generation cephalosporins. As a result, ESBL-producing bacteria are associated with high levels of morbidity and mortality in infected individuals. This study investigated the molecu...
Article
Full-text available
Aflatoxins (AFs) are secondary metabolites having a high cytotoxic potential and have an active carcinogenic compound. This study aimed to isolate hematopoietic stem cells (Precursors of mononuclear stem cells) from human umbilical cord blood. Collection of umbilical cord blood samples from cord blood was accomplished immediately after delivery, wh...
Preprint
Full-text available
Aflatoxins (AFs) are secondary metabolites having a high cytotoxic potential and have an active carcinogenic compound. This study aimed to isolate hematopoietic stem cells (Precursors of mononuclear stem cells) from human umbilical cord blood. Collection of umbilical cord blood samples from cord blood was accomplished immediately after delivery, wh...
Preprint
Full-text available
SEN-Virus is a DNA virus which is related to acute post-transfusion hepatitis and the prevalence with the clinical importance and also the genetic characterization are still much unknown in Iraq; therefore, this study was designed. This study was included three main approaches, the first is diagnostic, at which determination of hepatitis C virus in...
Thesis
Full-text available
SEN-Virus is a DNA virus which is related with acute post-transfusion hepatitis and the prevalence with the clinical importance and also the genetic characterization are still much unknown in Iraq; therefore, this study was designed. This study was included three main approaches, the first is the diagnostic, at which determination of hepatitis C vi...
Article
Full-text available
Objective and Background: SEN virus has identified as a putative new hepatitis virus coinfected with hepatitis C in thalassemic patients. The study aimed to measure viremia with SEN virus among thalassemic patients infected and non-infected with hepatitis C virus (HCV). Further, to detect the genotype D and the genetic alterations in the internal t...
Article
Full-text available
Background and Objective: The increase in extended-spectrum β-lactamases (ESBLs) producing microbes in recent years has led to the great challenge for the clinician in the treatment. The study aims to investigate the molecular basis of ESBL encoding-resistant gene, SHV to modern β-lactams. Also, to detect gene sequencing and to compare their geneti...
Article
Full-text available
Background and Objective: The most important tool which helps bacteria to tolerate survival in unwanted conditions and resistance to new generations of antimicrobial agents is biofilm formation through quorum sensing. This study aimed to detect biofilm formation using study isolates of staphylococci quantitatively. Further, concerning minimal inhib...
Data
The positive result for double disk synergy test between the AUG and CTX, CAZ among clinical study isolates. AUG-(amoxicillin + clavulanic acid, 30/10 μg), CTX-Cefotaxime (30 μg), CAZ-Ceftazidime (30 μg).
Preprint
Full-text available
The current study aimed to identify and check for the production of extended-spectrum beta-lactamase (ESBL) using double-disk synergy. Further, to investigate the molecular basis of ESBL encoding resistant genes, blaSHV, and blaVEB to new approach β-lactams. Furthermore, to detect gene sequencing and compare their genetic relatedness using nearest...
Preprint
Full-text available
Background and Objective: The increase in extended-spectrum β-lactamases (ESBLs) producing microbes in recent years has led to the great challenge for the clinician in the treatment. The study aims to investigate the molecular basis of ESBL encoding-resistant gene, SHV to modern β-lactams. Also, to detect gene sequencing and to compare their geneti...
Preprint
Full-text available
Objective and Background: SEN virus has identified as a putative new hepatitis virus coinfected with hepatitis C in thalassemic patients. The study aimed to measure viremia with SEN virus among thalassemic patients infected and non-infected with hepatitis C virus (HCV). Further, to detect the genotype D and the genetic alterations in the internal t...
Data
The comparison of susceptibility parameters for ciprofloxacin against logarithmic phase planktonic and sessile cells of study isolates of Staphylococcus aureus and staphylococcus epidermidis. With regard to ciprofloxacin, the BIC for SA1, SA4, SA7, SA9, SA 10, SA11, SA18,SE5, SE6, SE8, SE10, and SE12 study isolates were 50 (100 XMIC), 200 (100 XMIC...
Data
The member of aminoglycosides and amikacin inhibits Staphylococcus biofilm at the following inhibitory concentrations: 800, 200, 400, 1000, 1000, 200, 2000, 100, 1000, 1000, 400, and 1000 µg/ml. It was reduced the viable counts for these isolates markedly as observed in Table 6, but these concentrations were not enough to remove bacterial adherence...
Presentation
Full-text available
The presentation reflects the molecular detection of medically important and updated Metallo- β-lactamases which are represented in the article published in Scopus indexed Journal in 2018. I will attach with this presentation the full-text journal if someone need to cite this work with my pleasure
Article
Full-text available
Blood transfusion is associated with the risk of infection, especially hepatitis C virus and may lead to peroxidative tissue injury by secondary iron overload in - thalassemic patients. This study aims to detect HCV-RNA copies in thalassemic patients detected by RT-PCR qualitatively and quantitatively. Also, to measure the role of enzymatic and no...
Preprint
Full-text available
Background and objectives: Acute upper respiratory tract infections are the most frequent acute illnesses worldwide. This study been undertaken for determining the diagnostic importance of Human Rhinovirus and other associated DNA/RNA viruses including human Respiratory Syncytial virus; Human Metapneumovirus; Human parainfluenza virus 1-4; human co...
Article
Full-text available
Background and objectives: Acute upper respiratory tract infections are the most frequent acute illnesses worldwide. This study been undertaken for determining the diagnostic importance of Human Rhinovirus and other associated DNA/RNA viruses including human Respiratory Syncytial virus; Human Metapneumovirus; Human parainfluenza virus 1-4; human co...
Article
Full-text available
Background and Objectives: It is well realized that cervical cancer is one of the most critical diseases in the world. This study has been laid down for molecular detection (qualitatively and quantitatively) and genotyping of high and intermediate risk HPV genotypes in patients with high suspicion of cervical cancer. Patients and Methods: A total o...
Article
Full-text available
Background and Objectives: It is well realized that cervical cancer is one of the most critical diseases in the world. This study has been laid down for molecular detection (qualitatively and quantitatively) and genotyping of high and intermediate risk HPV genotypes in patients with high suspicion of cervical cancer. Patients and Methods: A total o...
Article
Full-text available
Background and Objectives: Acute upper respiratory tract infections are the most frequent acute illnesses worldwide. This study has been undertaken for determining the diagnostic importance of Human Rhinovirus and other associated DNA/RNA viruses including human Respiratory Syncytial virus; Human Metapneumovirus; Human parainfluenza virus 1-4; huma...
Data
Background and objectives: Acute upper respiratory tract infections are the most frequent acute illnesses worldwide. This study been undertaken for determining the diagnostic importance of Human Rhinovirus and other associated DNA/RNA viruses including human Respiratory Syncytial virus; Human Metapneumovirus; Human parainfluenza virus 1-4; human co...
Data
Acute upper respiratory tract infections are the most frequent acute illnesses worldwide. This study been undertaken for determining the diagnostic importance of Human Rhinovirus and other associated DNA/RNA viruses including human Respiratory Syncytial virus; Human Metapneumovirus; Human parainfluenza virus 1-4; human coronavirus; Human B, C and E...
Preprint
Full-text available
Background and Objective: The most important tool which helps bacteria to tolerate survival in unwanted conditions and resistance to new generations of antimicrobial agents is biofilm formation through quorum sensing. This study aimed to detect biofilm formation using study isolates of staphylococci quantitatively. Further, concerning minimal inhib...