Murilo Camuri Crovace

Federal University of São Carlos | UFSCar · Departamento de Engenharia de Materiais (DEMa)

This study aimed to evaluate the pulp response to F18 and cobalt-doped F18 bioglass (F18Co) in comparison with calcium hydroxide (CH) after pulp capping. The maxillary first molars of 48 rats were divided into F18, F18Co, CH, and control (no intervention) groups. The pulp was exposed, the materials were placed, and the teeth were capped. After 7 an...

Polymer composites with continuous fibers are expected to exhibit good mechanical performance due to orientation and high aspect ratio of fillers. Fused filament fabrication (FFF) provides an affordable method for processing these materials as products with tunable architecture. By incorporating continuous bioactive fibers coated with biodegradable...

Aim To evaluate the influence of an experimental solution of cobalt-doped F18 bioactive glass (F18Co) on tissue repair following regenerative endodontic procedure (REP) in rat molars. Methodology The F18Co solution was prepared at a ratio of 1:5 F18Co powder to distilled water. The right or left upper first molars of 12 Wistar rats were used, wher...

We investigated the crystallization kinetics of 24.5CaO-24.5SrO-3.7B2O3-6TiO2-41.3SiO2 and 25CaO-25SrO-3.8B2O3-8TiO2-42.2SiO2 (mol%) borotitanosilicate glass powders (≤ 43µm), herein referred as T6 and T8, respectively, using differential scanning calorimetry (DSC - β = 2.5°C/min – 40°C/min) and scanning electron microscopy (SEM). The kinetic param...

Magnetic hyperthermia (MHT) is a therapy that uses the heat generated by a magnetic material for cancer treatment. Magnetite nanoparticles are the most used materials in MHT. However, magnetite has a high Curie temperature (Tc~580 °C), and its use may generate local superheating. To overcome this problem, strontium-doped lanthanum manganite could r...

Despite the crucial role of osteoclasts in the physiological process of bone repair, most bone tissue engineering strategies have focused on osteoblast-biomaterial interactions. Although Biosilicate® with two crystalline phases (BioS-2P) exhibits osteogenic properties and significant bone formation, its effects on osteoclasts are unknown. This stud...

Biosilicate® glass-ceramic (BioS) offers an attractive choice for the manufacturing of scaffolds because of their high bioactivity, non-toxicity, bactericidal activity and biodegradability. Despite these positive properties, Biosilicate® scaffolds that have been developed so far show low fracture strength, limiting their clinical application. For t...

The Ti6Al4V alloy is usually employed as a biomaterial, however, when in use, exhibits a few drawbacks such as corrosion, caused by the release of aluminum and vanadium ions besides the bioinert behavior. Bioactive coatings offer a barrier effect and bioactivity, promoting biocompatibility and osseointegration processes. The present work aims to st...

Biosilicate® glass-ceramics are among the most valid alternatives to 45S5 Bioglass. They combine a similar bioactivity and bioresorbability as the 45S5 with superior mechanical strength, owing to the crystallization of a Na–Ca silicate phase. This crystallization may be experienced upon viscous flow sintering of fine glass powders, thus configuring...

Bioactive glasses and glass-ceramics (GCs) effectively regenerate bone tissue, but most GCs show improved mechanical properties. In this work, we developed and tested a rarely studied bioactive glass composition (24.4K2O-26.9CaO-46.1SiO2-2.6P2O5 mol%, identified as 45S5-K) with different particle sizes and heating rates to obtain a sintered GC that...

Glass-ceramics have found widespread use in dentistry due to their favorable properties: biocompatibility, chemical inertness, high fracture strength and toughness, superior esthetics, color stability, and translucence. The objective of this work was to develop a new tough, strong and machinable glass-ceramic based on the lithium metasilicate (LS)...

Bioglass 45S5 is known for its ability to regenerate bone; but unfortunately, this glass cannot be fully densified by viscous flow due to its very high tendency for crystallization. In this work, we evaluate the sintering and bioactivity of Bioglass 45S5-based compositions in which Na2O is incrementally replaced by K2O. Our sintering tests demonstr...

L'invention concerne un procédé de préparation de greffons osseux consistant : (110) à préparer un Biosilicate®, (120) à infiltrer une barbotine de Biosilicate® dans le Biosilicate® de l'étape (110), (130) à brûler le Biosilicate® infiltré de l'étape (120), (140) à infiltrer le Biosilicate® brûlé de l'étape (130) avec du bioverre F18, (150) à brûle...

This study evaluated the gene expression profile of the human adipose‐derived stem cells (hASCs) grown on the Biosilicate®/F18 glass (BioS‐2P/F18) scaffolds. hASCs were cultured using the osteogenic medium (control), the scaffolds, and their ionic extract. We observed that ALP activity was higher in hASCs grown on the BioS‐2P/F18 scaffolds than in...

The Biosilicate® glass-ceramic is one of the most promising alternatives to the 45S5 Bioglass® in terms of bioactivity, osteoconductivity, osteoinductivity, non-cytotoxicity, and antibacterial properties, with significant advantages in the manufacturing of specific components of complex shapes for bone tissue application. Unlike in 45S5 glass, the...

After five decades of research on bioactive glasses and glass-ceramics, these materials became of considerable interest due to their revolutionary potential for numerous health applications, including cancer treatment. One advantage of glass-ceramics compared with other materials – such as metallic alloys and polymers – is their capability of being...

Engineered stones are high-end materials increasingly being used as kitchen countertops, floor and facade tiles, as well as in other architectural applications. These materials consist of approximately 70–95 wt% of mineral particles (usually quartz) dispersed in a matrix made of cement, ceramics or, much more often, polymer resins. Despite their ve...

This study aimed to investigate the in vivo tissue response of the Biosilicate® scaffolds in a model of tibial bone defect. Sixty male Wistar rats were distributed into bone defect control group (CG) and Biosilicate® scaffold group (BG). Animals were euthanized 15, 30 and 45 days post-surgery. Stereomicroscopy, scanning electron microscopy, histopa...

Monolithic and powdered Biosilicate®, produced by conventional glass‐ceramic technology, have been widely recognized as excellent materials for bone tissue engineering applications. In the current research, we focus on an alternative processing route for this material, consisting of the thermal treatment of silicone polymers containing micro‐sized...

We aimed to evaluate the in vitro osteogenic and osteoinductive potentials of BioS-2P and its ability to promote in vivo bone repair. To investigate osteogenic potential, UMR-106 osteoblastic cells were cultured on BioS-2P and Bioglass 45S5 discs in osteogenic medium. The osteoinductive potential was evaluated using mesenchymal stem cells (MSCs) cu...

Calcium phosphates and bioactive glass ceramics have been considered promising biomaterials for use in surgeries. However, their moldability should be further enhanced. We here thereby report the handling, physicochemical features, and morphological characteristics of formulations consisting of carboxymethylcellulose–glycerol and hydroxyapatite-tri...

Para o reparo de defeitos ósseos, o uso da engenharia de tecidos, combinando células e biomateriais, tem se mostrado uma alternativa promissora. Nesse estudo, o arcabouço de Biosilicato® com duas fases cristalinas (Bio 2P) foi avaliado in vitro para determinar seu potencial osteogênico e osteoindutor e in vivo foi associado ou não a células para in...

The ability of Biosilicate® with two crystalline phases (BioS-2P) to drive osteoblast differentiation encourages the investigation of the cellular mechanisms involved in this process. Then, the aim of our study was to analyze the large-scale gene expression of osteoblasts grown on BioS-2P compared with Bioglass®45S5 (45S5). Osteoblasts differentiat...

Bioactive glass-ceramics have emerged as promising biomaterials to produce scaffolds for bone tissue engineering thanks to their physical properties and stimulatory effects on osteoblast differentiation. Among the biomaterials, bioactive glass-ceramics emerge as a promising scaffold due to their physical properties and the stimulatory effects on os...

Introduction. Bone tissue repair can be achieved through tissue engineering strategies where cells are carried to bone defects within a porous scaffold. In this study we evaluated the effect of combining mesenchymal stem cells (MSCs) or osteoblasts (OBs) with a bioactive glass scaffold (Bio-sca) on repair of bone defects created in rat calvarias. M...

This study evaluated the effects of the Biosilicate(®) and poly (D,L-lactic-co-glycolic) acid composites on bone repair in a tibial bone defect model in rats by means of using histological evaluation (histopathological and morphometric analysis) and gene expression analysis. Eighty male Wistar rats (12 weeks old, weighing ±300 g) were randomly divi...

The aim of this study was to evaluate the in vitro and in vivo osteogenic potential of the bioactive glass-ceramic scaffold (Bio-Sca). Rat mesenchymal stem cells (MSCs) were cultured on either dense Bio-Sca discs and or polystyrene (control) under non-osteogenic conditions. At day 10, alkaline phosphatase (ALP) activity and gene expression of the b...

The purpose of this study was to analyze olfactory ensheathing cell (OEC) proliferation and growth on Biosilicate and collagen bioscaffolds, and to determine whether the application of laser phototherapy would result in increased OEC proliferation on the scaffolds. The use of bioscaffolds is considered a promising strategy in a number of clinical a...

J Appl Biomater Funct Mater. 2015 May 29:0. doi: 10.5301/jabfm.5000220. [Epub ahead of print] Effect of 830-nm laser phototherapy on olfactory neuronal ensheathing cells grown in vitro on novel bioscaffolds. Renno AC1, McDonnell PA, Crovace MC, Zanotto ED, Laakso EL. Author information Abstract BACKGROUND: The purpose of this study was to analyze o...

J Appl Biomater Funct Mater. 2015 May 29:0. doi: 10.5301/jabfm.5000220. [Epub ahead of print] Effect of 830-nm laser phototherapy on olfactory neuronal ensheathing cells grown in vitro on novel bioscaffolds. Renno AC1, McDonnell PA, Crovace MC, Zanotto ED, Laakso EL. Author information Abstract BACKGROUND: The purpose of this study was to analyze o...

Biosilicate® and Bio-Oss® are two commercially available bone substitutes, however, little is known regarding their efficacy in osteoporotic conditions. The purpose of this study was to evaluate the osteogenic properties of both materials, at tissue and molecular level. Thirty-six Wistar rats were submitted to ovariectomy (OVX) for inducing osteopo...

In this review we critically analyze 28 theses and dissertations and over 30 scientific papers that tested Biosilicate®, a highly bioactive glass-ceramic, in a number of applications throughout the past 20 years. Biosilicate® presents a combination of positive features for bone tissue regeneration: it is highly bioactive, osteoconductive, osteoindu...

Biosilicate® and Bio-Oss® are two commercially available bone substitutes, however, little is known regarding their efficacy in osteoporotic conditions. The purpose of this study was to evaluate the osteogenic properties of both materials, at tissue and molecular level. Thirty-six Wistar rats were submitted to ovariectomy (OVX) for inducing osteopo...

The aim of this study was to evaluate the effects of highly porous Biosilicate® scaffolds on bone healing in a tibial bone defect model in rats by means of histological evaluation (histopathological and immunohistochemistry analysis) of the bone callus and the systemic inflammatory response (immunoenzymatic assay). Eighty Wistar rats (12 weeks-old,...

In this study, lithium disilicate (LS2) glass samples with different particle sizes ranging from less than 105 to 850 μm were prepared. These specimens were inserted in a Pt-Rh DSC crucible and heated to 850°C at different rates (ϕ = 0.5–30 K/min) to identify their crystallization peaks. The activation energies for the overall crystallization (E) a...

The bioactive glass 45S5 favours osteoblast activities resulting in enhanced osteogenesis both in vitro and in vivo1, but the relative poor mechanical properties restrict its use as bone substitute 2,3. In this context, novel full-crystallized bioactive glass-ceramics, Biosilicate® (Bio) and Biosilicate® for scaffold fabrication (Bio-sca), has been...

The increasing clinical demand for bone substitutes has driven significant progress in bone tissue engineering. Among the biomaterials that have been employed as scaffolds, bioactive glasses emerge as a novel alternative due to their high biocompatibility and ability to stimulate in vitro osteogenesis. Despite the advantages on bone healing, the us...

There is a strong discrepancy in the literature regarding the effect of magnesium on bioactive glasses. Hence the present study is focused on the physical and chemical behavior of the "golden standard" 45S5 glass and magnesium-containing bioactive glasses developed here to evaluate their reactivity and in vitro bioactivity. The aim of this study wa...

The development of bioactive glass-ceramic materials has been a topic of great interest aiming at enhancing the mechanical strength of traditional bioactive scaffolds. In the present study, we test and demonstrate the use of Biosilicate® glass-ceramic powder to fabricate bone scaffolds by the foam replica method. Scaffolds possessing the main requi...

The mechanical strength of bioactive glasses can be improved by controlled crystallization, turning its use as bulk bone implants viable. However, crystallization may affect the bioactivity of the material. The aim of this study was to develop glass-ceramics of the nominal composition (wt%) 52.75(3CaO·P2O5)-30SiO2-17.25MgO, with different crystalli...

Objective: This study aimed to investigate the in vivo tissue performance of the association of Biosilicate(®) scaffolds and low-level laser therapy (LLLT) in a tibial bone defects model in rats. Background data: Many studies have been demonstrating the osteogenic potential of Biosilicate and LLLT. However, there is a need to investigate the eff...

This study evaluated the biocompatibility of Biosilicate® scaffolds by means of histopathological, cytotoxicity, and genotoxicity analysis. The histopathologic analysis of the biomaterial was performed using 65 male rats, distributed into the groups: control and Biosilicate®, evaluated at 7, 15, 30, 45, and 60 days after implantation. The cytotoxic...

Calcium phosphate cements (CPCs) have been widely used as an alternative to biological grafts due to their excellent osteoconductive properties. Although degradation has been improved by using poly(D,L-lactic-co-glycolic) acid (PLGA) microspheres as porogens, the biological performance of CPC/PLGA composites is insufficient to stimulate bone healin...

After an introduction showing the growing interest in glasses and glass-ceramics as biomaterials used for bone healing, we describe a new biomaterial named Biosilicate. Biosilicate is the designation of a group of fully crystallized glass-ceramics of the Na2O-CaO-SiO2-P2O5 system. Several in vitro tests have shown that Biosilicate is a very active...

Bioactive glasses (BG) are known for their unique ability to bond to living bone. Consequently, the incorporation of BG into calcium phosphate cement (CPC) was hypothesized to be a feasible approach to improve the biological performance of CPC. Previously, it has been demonstrated that BG can successfully be introduced into CPC, with or without PLG...

Objective: The purpose of this study was (i) to develop a method for successfully seeding osteoblasts onto a glass-ceramic scaffold designed for use in clinical settings, and (ii) to determine whether the application of laser phototherapy at 830 nm would result in osteoblast proliferation on the glass-ceramic scaffold. Background: The use of biosca...

The purpose of this study was (i) to develop a method for successfully seeding osteoblasts onto a glass-ceramic scaffold designed for use in clinical settings, and (ii) to determine whether the application of laser phototherapy at 830 nm would result in osteoblast proliferation on the glass-ceramic scaffold. The use of bioscaffolds is considered a...

In the present work surface and bulk residual stresses generated in partially crystallized Li2O · 2SiO2 glass–ceramics are analyzed after different heat treatments. The phase specific residual stresses in the crystalline Li2Si2O5-phase are evaluated for the first time in the near-surface zone and the bulk of the samples using both medium and high e...

In the present work, the electrical conductivity of doped HVDC insulator glasses was determined using the impedance spectroscopy method in a temperature range of 350-550ºC. The glass transition temperature (Tg) of these glasses was also verified through a Differential Scanning Calorimetry (DSC) analysis. Resumo. Neste trabalho, a condutividade elét...

In the present work surface and bulk residual stresses generated in partially crystallized Li 2 O AE 2SiO 2 glass–ceramics are analyzed after different heat treatments. The phase specific residual stresses in the crystalline Li 2 Si 2 O 5-phase are evaluated for the first time in the near-surface zone and the bulk of the samples using both medium a...