Mukesh Kumar

Central Institute of Fisheries Education | CIFE · Division of Fisheries Genetics & Biotechnology

Imposing lockdown amid COVID-19 pandemic has severely affected flower cultivation and their trades. Flower plants are very sensitive to the harvesting, and any unexpected delay may cause great loss (~ 50–60%) to the farmers. In 2018–2019, the worth of total production of floriculture products was ~ Rs 571.38 crore. During lockdown, the availability...

Aim To evaluate the binding affinity and biological potency of gonadotropin-releasing hormone analogue (GnRHa) Buserelin (C60H86N16O13) based on in silico and in vivo testing for induced breeding in Clarias magur. Background Many attempts have been made to induce C. magur, but encouraging results have not yet been achieved. Hence, it is the need o...

Background/aims: GnIH receptors (GnIHRs) belong to the family of G-protein coupled receptors (GPCRs) and play a key role in the regulation of reproduction from fishes to mammals, either by inhibiting or stimulating the expression of gonadotropins. The aim of this study was to characterize GnIH receptor (GnIHR2) from Indian Major Carp, Labeo catla...

Novel strategies have been developed for the identification of full-length cDNA using bioinformatics tools and multiplexed PCR methods. Usually, sequences are either incomplete or have missing UTR-sequences. Researchers still use the Rapid Amplification of cDNA Ends technique to obtain the full-length cDNA sequences. An oligo(dT) anchor-primer with...

An experiment was designed to delineate the efficacy of a dietary mixture of selenium nanoparticles (Se-NPs) and riboflavin (RF) on the thermal efficiency/tolerance of Pangasianodon hypophthalmus reared under arsenic (2.8 mg/L) and high-temperature (34 °C) stress. A green synthesis method was employed for the synthesis of Se-NPs using fish gills, w...

Microbes are an essential component of any ecosystem and play a critical role in maintaining a healthy balance. Metagenomics offers an opportunity to explore the hidden world of microbes of both cultivable and non-cultivable nature through genetic investigations. Different aspects of microbiome study are available such as metagenomics and meta tran...

Quality of Basmati rice is largely deteriorated due to admixture of non-Basmati grains and genotype x environment (GxE) interactions within and beyond geographical indication (GI) area. Traditional morpho-physico-chemical methods supplemented with sensory evaluation could determine the deterioration in quality of Basmati rice resulted from GxE inte...

The Insilco experiment was conducted to find out small drug or ligand which can able to bind Gonadotropin releasing hormone receptor of Monopterus cuchia. GnRH receptor are G protein coupled receptor for this purpose ligand selected on basis of GPCR ligand, Ion channel modulator, Kinase inhibitor, Nuclear receptor ligand, Protease inhibitor, Enzyme...

A 45-day experiment trial was conducted to determine the effect of graded levels of dietary ginger supplementation on growth and nutrient utilization of Cyprinuscarpio (Linnaeus; 1758) fingerlings. Fishes of uniform size (average weight 6.0-6.5 g) were divided into six experimental groups in triplicates following completely randomized design (CRD)....

The development and utilization of biochemical and molecular markers are becoming widely used in floricultural crops for varying purposes including genetic studies, qualitative as well as quantitative traits, genotype fingerprinting, phylogenetic studies and mapping populations.

Cell lines are a valuable biological tool for carrying out researches in physiology, virology, pharmacology, toxicology, cancer/carcinogenesis and transgenic fields. Several "fish cell lines" have been developed from a broad range of tissues by explant techniques, e.g., ovary, fin, swim bladder, heart, spleen, liver, eye muscle, vertebrae, brain, s...

Vertebrates may not be killed without effective elimination of pain perception. Likewise, any kind of experimental intervention may not be undertaken without appropriate anesthesia. In cases where appropriate anesthesia is not possible, then the pain should be reduced to a minimum (analgesia). While generally, a veterinarian must apply anesthesia i...