Mukai Yoshiharu

• Showa University

Purpose: To investigate the antimicrobial effects of a mouthrinse containing silver nanoparticles (AgNP) on polymicrobial biofilms in vitro. Materials and methods: Polymicrobial biofilms were grown on glass cover slips following the method of Exterkate. Saliva collected from a healthy human was added to McBain medium (including 0.2% sucrose) to...

Purpose: To analyze the effects of surface pre-reacted glass-ionomer (S-PRG) filler eluate on polymicrobial biofilm metabolism and live bacterial count. Methods: Biofilm was formed using glass disks 12 mm in diameter and 150 µm in thickness. Stimulated saliva was diluted 50-fold with buffered McBain 2005 and cultured in anaerobic conditions at 3...

The aim of this study was to investigate the mineral-promoting effects of in-office bleaching agent on enamel subsurface lesions. Enamel subsurface lesions were divided into following groups; D: demineralized samples without any further treatment, DS: samples were further immersed in fresh saliva, DSR: samples were immersed in saliva followed by re...

We investigated the remineralization effects of Nanoseal (NS) dentin desensitizer on demineralized root dentin. Baseline lesion specimens prepared from bovine root dentin were immersed in artificial saliva (AS) or deionized water (DW) after treatment with NS or fluoride-free Nanoseal (NS(−)). Treatment and control groups comprised: 1, AS; 2, NS/AS;...

This study investigated the anti-demineralization effects of surface pre-reacted glass-ionomer (S-PRG) filler-containing varnishes. Thirty-five bovine root specimens were divided into five treatment groups, with seven specimens each coated with 1) MI varnish (MIV), 2) F varnish (FV), 3) PRG varnish I (PV), 4) PRG varnish II (with sodium fluoride ad...

Purpose: To analyze changes in pH and bacterial flora with duration of culture and timing of sugar supply using a polymicrobial biofilm model. Methods: The biofilm was prepared using the method of Exterkate et al. Stimulated saliva from an adult was collected on a glass slide and added to unbuffered McBain medium containing 0.2% sucrose and cult...

Objective: To evaluate anti-demineralization effects of dentin desensitizer containing sodium fluoride and methacrylate-co-p-styrene sulfonic acid (MS polymer) on root dentin using transverse microradiography (TMR). Material and methods: Twenty-four dentin specimens were divided into four groups: MSO (no fluoride), MSF (3000 ppm F), FJL (9000 ppm F...

Purpose: To compare the efficacy of toothpaste containing surface pre-reacted glass-ionomer (S-PRG) filler particles to that of conventional sodium fluoride (NaF) toothpaste for the prevention of dentin demineralization and biofilm regrowth. Methods: Bovine root dentin specimens and glass coverslips were used as biofilm growth substrates. To est...

Purpose: To investigate the effects of in-office bleaching on the remineralization of enamel lesions filled with organic components of red wine. Methods: Enamel specimens were exposed to 0.1% NaF solution for 1 minute immersed in red wine for 5 days at 37°C, and subjected to in-office bleaching followed by remineralization in 1.5 mM CaCl₂, 0.9 m...

We used a polymicrobial (PM) biofilm model to examine associations of bacterial adhesiveness with surface characteristics of various dental materials. Four types of dental materials (apatite pellet, zirconia, ceramic, and composite resin) with rough and mirror surfaces were used. Surface roughness, surface free energy, zeta potential, and colony-fo...

The purpose of this study was to evaluate the adherence and detachment of bacterial biofilms formed on glass surface modified with 10F2S unmodified cover glasses were imme and theywere cultured to develop PM biofilms. There were separated six experimental groups (n = 6), designated WC: cover glass washing withCPW, WI: modified with 10F2S with CPW,...

In the present study, we investigated, using micro-Raman spectroscopy (Raman) and transverse microradiography, the influence of bicarbonate [sodium hydrogen carbonate (NaHCO3)] on the effects of carbonate ions in the mineral phase during demineralization (acid resistance test) of subsurface lesions. Baseline lesions were created by demineralizing b...

This study investigated the in vitro anti-demineralization effects of resin-based temporary filling materials containing surface prereacted glass-ionomer (S-PRG) filler on dentin. Bovine root dentin specimens with a 3×3 mm experimental surface were divided into four treatment groups: DuraSeal (DU) as a control, S-PRG filler-free temporary material...

The purpose of this study was to evaluate the antibacterial activity against polymicrobial (PM) biofilms of a condensed tannin extracted from astringent persimmon (PS-M), which is contained in refreshing beverages commercially available in Japan. Salivary PM biofilms were formed anaerobically on glass coverslips for 24 and 72 h and were treated for...

Aim: Elderly individuals face the risk of reductions in saliva secretion and occlusal force caused by systemic diseases or medications that can eventually result in malnutrition and systemic complications. We tested the hypothesis that regular gum chewing exercises (GCE) would enhance saliva secretion and occlusal force in an elderly population....

Background/purpose In order to obtain information for the design of foods for infants and other young children, we analyzed bite size and mastication of children eating test foods with their fingers. Methods Using baked stick biscuits (Food A: newly designed biscuits for children; or Food B: commercially available control biscuits) as the test foo...

To simulate an oral demineralization environment by multiple species of bacteria by inducing subsurface dentin lesions with a polymicrobial biofilm model. Polymicrobial biofilms consisting of multiple species of bacteria were generated from stimulated saliva using a high-throughput active attachment model. Biofilms were grown on dentin specimens in...

To investigate the in vitro antimicrobial effects of carbamide peroxide (CP) and CP-based home bleaching agents against polymicrobial (PM) biofilms. Using a high-throughput active attachment model, PM biofilms were cultured on glass coverslips by diluting the stimulated saliva of one healthy adult. All experiments were performed anaerobically in Mc...

In April 2012, the Center of Special Needs Dentistry (SND) was established at Showa University Dental Hospital to provide function training for children with eating and swallowing disorders. A statistical clinical assessment was performed on new patients ≤18 years of age who visited the Center over a 1-year period (April 2012–March 2013) to assess...

Objective Respiratory disease occurs frequently in children. The nasal cavity, trachea, and oral cavity are considered the primary routes of infection. We aimed to investigate the types of microbial pathogens present in the oral cavities of children with respiratory disease. In addition, we compared the detection rates of different pathogens betwee...

Salivary macromolecules infiltrate white and brown spot enamel lesions and adsorb onto hydroxyapatite. Calcium-binding salivary proteins such as statherin hinder remineralization of these lesions. We assessed whether bleaching agents can remove salivary components that have infiltrated and bound to experimental subsurface lesions in bovine enamel p...

A fluoride-releasing coating material containing surface pre-reacted glass-ionomer (S-PRG) filler has become commercially available. However, there has been no detailed investigation of its remineralization effects at various tooth surface regions. The remineralization effects of S-PRG filler-containing coating material at different sites of demine...

It is well known that children with autistic spectrum disorder possess various behavioral problems related to feeding, short attention span and maladaptation to surrounding environments. This study aimed to identify appropriate measures to address behavioral characteristics associated with eating and feeding in children with ASD by examining feedin...

Saliva contains mucins, which protect epithelial cells. We showed a smaller amount of salivary mucin, both MG1 and MG2, in the premenopausal female smokers than in their nonsmoking counterparts. Smokers' MG1, which contains almost 2% cysteine/half cystine in its amino acid residues, turned out to be chemically altered in the nonsmoker's saliva. The...

To develop strategies to support feeding and swallowing functions from early stage, we evaluated chief complaints regarding feeding, and assessment of the feeding function. The subjects consisted of 53 preschool children and their guardians, who participated in feeding consultation in a welfare center. In this study, we extracted chief complaints a...

Background: Despite resolution of the symptoms of eating and/or swallowing disorders, prolonged tube feeding is maintained in some children. This study summarized the characteristics of children with tube dependence and investigated the causes of tube dependence. Methods: Clinical and growth data were evaluated using medical records and referral...

To investigate the laboratory anti-demineralization effect of a novel fluoride-releasing varnish containing surface reaction-type prereacted glass-ionomer (S-PRG) filler. Paired specimens were cut from bovine root dentin. One of each pair was used for the S-PRG group, and the other served as a control (n = 6). A 1 x 3 mm test surface was made on ea...

The aim of this study was to consolidate oral health management systems in the acute stages. The practical status of oral health in perioperative patients and improvements achieved through coordinated oral health care were investigated. Subjects of the present study were 87 patients who underwent oral intubation in the intensive care unit (ICU). Th...

The Oral Health Care Center at Showa Universityʼs Fujigaoka Hospital fully implemented a clinical pathway for oral health care in cardiovascular surgery in September 2008. Activities of the Oral Health Care Center currently cover all wards. Oral health care was offered on a trial basis at Fujigaoka Rehabilitation Hospital in December 2009 and is cu...

Studies on feeding behaviors of children with pervasive developmental disorders (PDD) have focused mainly on problematic behaviors such as food selectivity and how to deal with them. Few studies have addressed the motor aspects of feeding behavior. This study aimed to determine the characteristics of feeding behavior of children with PDD using a sp...

The purpose of this study was to examine the effectiveness of medical support for children with autistic spectrum disorder (ASD) concerning their eating problems in rehabilitation and at home using a survey of eating conditions and assessment of eating and swallowing functions.We studied chief complaints, assessment of eating and swallowing functio...

This study tested the hypothesis that the benefit of fluoride-releasing restorative materials continues even after their reserve of fluoride has been depleted. Pits in perspex blocks simulating cavities were filled with either a fluoride-releasing or a non-fluoride-releasing restorative material and a dentin single-section was placed 1 mm from the...

Recently, our society is rapidly aging, and it's getting more important to evaluate swallowing function for preventing accidents and illness such as aspiration pneumonia. So we developed a new evaluation system for swallowing function. This system can evaluate swallowing function safely and quantitatively without touching by a Fiber grating vision...

唇顎口蓋裂は発生頻度の高い先天異常であり, 治療や療育にはさまざまな分野からのアプローチが必要とされているが, 摂食や口唇閉鎖などの口腔機能面でのアプローチは未だ十分に行われていない. そこで, 唇顎口蓋裂児の口腔機能の発達を解明するために, 小型圧センサを埋め込んだスプーンを用いて片側性唇顎口蓋裂児の口唇正中部, 口唇側方部 (破裂側および非破裂側) の3点から捕食時垂直的口唇圧の測定を行った. 対象は乳歯列完成期の片側性唇顎口蓋裂児 (Cleft群) 23名で, 健常小児 (N群) 25名を対照群とした. 解析項目は, 捕食時口唇圧, 陰圧, 捕食時作用時間, 捕食時圧積分値とし, それぞれについて検討を行った. 捕食時口唇圧はCleft群がN群と比較して正中部で有意に小さかった. 陰圧...

Recently airway blockage accidents have increased in Japan, and airway blockage accidents involving children account for 12% of the total. However, the causes of airway blockage accidents are not clear. The oropharynx plays an important role in feeding and swallowing by helping to prevent aspiration and airway blockage accidents. Few studies, howev...

Recently airway blockage accidents have increased in Japan, and airway blockage accidents involving children account for 12% of the total. However, the causes of airway blockage accidents are not clear. The oropharynx plays an important role in feeding and swallowing by helping to prevent aspiration and airway blockage accidents. Few studies, howev...

In the present study we examined the effects of neonatal orosensory deprivation on taste-elicited gustatory activity in the rat parabrachial nucleus (PBN) using the functional anatomical marker c-Fos. Animals in three groups (GG, GO and GM) received gastric cannula implantation surgery on postnatal day 9 (P9). Animals in the fourth group (MR) did n...

The purpose of this study was to confirm the relationship between jaw bone growth and period of non-oral feeding with gastric infusion in weaning rats by measuring the bone growth using high-resolution computed tomography (micro-CT). Sprague-Dawley (SD) rats underwent gastrostomy at postnatal 7 (P7),11, and 14 days. Five rat pups were included in e...

The demineralization of dentin was studied when placed adjacent to one of four experimental denture base-resins. These experimental resins contained polymethylmethacrylate (PMMA) and 0, 5, 10, 20 or 30 wt% surface reaction-type prereacted glass-ionomer (S-PRG) filler, respectively. A dentin thin-section was sandwiched between these resins and subje...

To obtain a better understanding of the changes in feeding behaviour from 1 to 6 months of age. By comparing breast- and bottle-feeding, we intended to clarify the difference in longitudinal sucking performance. Sucking variables were consecutively measured for 16 breast-fed and eight bottle-fed infants at 1, 3 and 6 months of age. For breast-feedi...

We sought to determine if different mammary lobes produce milk of varying composition. Seventeen mothers were enrolled in this study. Foremilk was obtained from three nipple openings on each breast. After the breastfeeding session, hindmilk was sampled in the same manner. We measured creamatocrit (CrCt) and analyzed the protein concentration. Data...

The purpose of this study was to evaluate the influence of a bottled green tea beverage on dentin demineralization with a demineralization gel system in vitro. Samples were cut from bovine root dentin. Each sample was immersed in 8% methylcellulose gel onto which a layer of green tea beverage was placed. For comparison, sugar-free coffee beverage,...

The flexural strength, flexural modulus, and the amount of fluoride released from four experimental denture base resins containing 5, 10, 20 and 30 wt% surface pre-reacted glass-ionomer (S-PRG) filler added to the powder were evaluated. The mean flexural strength of the experimental resins, except the 30 wt%, and the flexural modulus of all the res...

The aim of this study was to evaluate the effect of artificial saliva during enamel bleaching for 12 weeks in vitro. Cut bovine incisors were classified into six groups. In Group C, enamel specimens were immersed in artificial saliva (pH 6.5) for 12 weeks as a control. In Groups Hw and Hr, the enamel specimens were bleached with HiLite (containing...

It is known that the fat content of breast milk is higher in hindmilk than in foremilk. However, it has not been determined if this increased fat content results from an increase in the number of milk fat globules (MFGs), an increase in the size of MFGs, or both. This study aims to determine which factor plays the most important role. Thirteen brea...

It is known that anxiety levels of parents regarding the feeding activities of their children peak just after birth, and then such anxiety continues until 1 or 2 years old, when weaning is completed. The purpose of this study was to aid in the establishment of a new teaching and support system for toddlers aiming not to improve existing organic asp...

The effect of fluoride-containing orthodontic resin bonding systems on acid resistance of adjacent enamel was evaluated using transversal microradiography. Four fluoride-containing orthodontic resin bonding systems: BeautyOrtho Bond (BO), BeautyOrtho Bond+Salivatect (BOS), Kurasper F (KP), Transbond XT (TB) and a resin-modified glass-ionomer (Fuji...

The purpose of this study was to compare the profiles of transversal microradiography and nano-hardness with remarkably remineralized enamel lesions under conditions of non-fluoride. Furthermore, acid resistibility of remineralized enamel was investigated by Raman microspectroscopy. Demineralized bovine enamel was remineralized for 6 and 12 weeks,...

An equation has been proposed describing the relation between √KHN and mineral content for demineralized enamel (vol%=4.3(√KHN)+11.3). However, it is unknown whether mineral content profiles of remineralized enamel are equivalent to hardness profiles. Our objective was to compare profiles of transversal microradiography and hardness for remineraliz...

The purpose of this study was to investigate the features of hyoid movement during swallowing in the pharyngeal stage, and to devise an effective rehabilitation method for disabled patients. The subjects of the study were ten persons with cerebral palsy (CP : 3 years 9 months-34 years 9 months) and eight persons with mental retardation (MR : 2 year...

To improve feeding and swallowing, a high-specialty functional therapy, which mainly focuses on thedecreased functions due to advanced age, is under development. In the present study, a 12-week programto improve the oral function was performed by using simplified tools. The aim was to develop a novel system for improving the oral function in care-r...

The purpose of this study was to analyze the actual condition of eating independence and the oral health status to establish a more effective therapy and support for residents with dysphagia. The subjects were 141 individuals (36 males and 105 females; mean age of 79.4, 9.0 SD), who had eating and swallowing therapy at nursing homes for five years...

This study evaluated the capability of a novel fluoride-releasing, all-in-one adhesive system on forming inhibition layer (radio-opaque layer) as compared with other adhesive systems. Dentin surface was treated with Imperva bond (IB), FL-BOND (FB), Reactmer bond (RE), or FL-BOND S-1 (FS) (which is a novel system). Untreated specimens were categoriz...

The electrophysiological properties of jaw-closing motoneurons (JCMNs) in postnatally developing rats aged 4 to 27 days were investigated in slice preparations using intracellular recording techniques. The input resistance of JCMNs from young rats (aged 4-9 days) was greater than that of JCMNs from rats aged 10 days or older. The action potential h...

The purpose of this study was to evaluate the durability of tubules occluded with FTLA treatment by toothbrush abrasion test on the applied surface and by measuring fluoride release from the FTLA components. Dentin specimens with simulated hypersensitive surfaces were treated with APF containing tannic acid. After which, the specimens received lant...

To simulate hypersensitive dentin, the smear layer and dentinal plugs of bovine root dentin specimens were removed by immersion in 10% phosphoric acid, polishing with hydroxyapatite particles, and ultrasonic cleansing. The fluoride-tannic acid-lanthanum-apatite (FTLA) group was treated with acidulated phosphate fluoride (APF) containing tannic acid...

The present paper is concerned with a new finding with regard to the inactivation and the nasal bioavailability of IFN-β in two different powder dosage forms, i.e. mixed powder (MP) and freeze-dried powder (FP) dosage forms. The two types of preparations which contained bile salts were prepared. The IFN-β titer was determined by bioassay and enzyme...

Two dextran derivatives, fluorescein isothiocyanate-dextran (DT) and fluorescein isothiocyanate diethylaminoethyldextran (DE), were administered intranasally to study how the molecular weight and charge influence permeation through nasal mucosa in rabbits. These compounds were used as macromolecular models of water-soluble drugs. The concentration...