Muhammad Safiullah Virk- Doctor of Philosophy
- PostDoc Position at Jiangsu University
Muhammad Safiullah Virk
- Doctor of Philosophy
- PostDoc Position at Jiangsu University
About
23
Publications
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Introduction
Current institution
Publications
Publications (23)
This research aimed to evaluate the effect of triple-frequency ultrasound treatment (TFUT), germination (GE), and traditional soaking (TS) methods on the nutritional and techno-functional properties of two different barley varieties, including ZQ2000 and XMLY22. Both ZQ2000 and XMLY22 varieties exhibited the highest total phenolic content (TPC) wit...
Citrus fruits, classified under the Rutaceae family and Citrus genus, are valued for their high nutritional content, attributed to their rich array of natural bioactive compounds. To ensure both quality and nutritional value, precise non-destructive testing methods are crucial. Among these, computer vision and spectroscopy technologies have emerged...
Globally, 50% of people consume rice (Oryza sativa), which is among the most abundant and extensively ingested cereal grains. Rice bran is a by-product of the cereal industry and is also considered a beneficial waste product of the rice processing industry. Rice bran oil (RBO) is created from rice bran (20–25 wt% in rice bran), which is the outermo...
Dietary oils (DOs) from various sources are known to be beneficial functional bioactives in medicinal applications. However, DOs are highly susceptible to light, temperature, and oxygen, rendering them chemically unstable and prone to oxidation. As a result, their use in the food and pharmaceutical sectors is constrained. Nanoencapsulation techniqu...
Several billion microorganisms reside in the gastrointestinal lumen, including viruses, bacteria, fungi, and yeast. Among them, probiotics were primarily used to cure digestive disorders such as intestinal infections and diarrhea; however, with a paradigm shift towards alleviating health through food, their importance is large. Moreover, recent stu...
Producing starch gels with superior mechanical attributes remains a challenging pursuit. This research sought to develop a simple method using ethanol exposure to produce robust starch gels. The gels’ mechanical properties, rheology, structural characteristics, and digestion were assessed through textural, rheological, structural, and in vitro dige...
The biosynthesis of penicillin G (PG) is compartmentalized, and the transportation of the end and intermediate products, and substrates (precursors) such as L-cysteine (L-Cys), L-valine (L-Val) and phenylacetic acid (PAA) requires traversing membrane barriers. However, the transportation system of PAA as a side chain of PG are unclear yet. To disco...
Monascus vinegar (MV), during whose brewing process Monascus spp. and polished rice (PR) are normally used as the starter and the raw material, respectively, is one of the traditional vinegars in China. In this study, the effects of three raw materials, including PR, unhusked rice (UR), and germinated UR (GR), on MV volatile compounds have been inv...
Monascus species have been used for the production of many industrially and medically important metabolites, most of which are polyketides produced by the action of polyketide synthases that use acetyl-CoA and malonyl-CoA as precursors, and some of them are derived from acetate. In this study the effects of acetic acid, and two kinds of acetates, s...
![Figure 11. UPLC-MS/MS ([M-H] − ) spectrogram of the M. ruber M7, ΔmfsT,...](publication/336104258/figure/fig3/AS:829894388903937@1574873649657/UPLC-MS-MS-M-H-spectrogram-of-the-M-ruber-M7-DmfsT-DmfsTmfsT-and_Q320.jpg)
: The biosynthesis of penicillin G (PG) is compartmentalized, which forces penicillin and its intermediates to cross the membrane barriers. Although many aspects around the penicillin intermediates traffic system remain unclosed, the transmembrane transporter protein involvement has been only predicted. In the present work, detection of PG and isop...
The present study aimed to elucidate the photosynthetic performance, antioxidant enzyme activities, anthocyanin contents, anthocyanin biosynthetic gene expression, and vanadium uptake in mustard genotypes (purple and green) that differ in photosynthetic capacity under vanadium stress. The results indicated that vanadium significantly reduced photos...
Salmonella infection is an important foodborne consumer health concern that can be mitigated during food processing. Bacteriophage therapy imparts many advantages over conventional chemical preservatives including pathogen specificity, natural derivation, potency, and providing a high degree of safety. The objective of this study aimed to isolate a...
The concentration of advanced glycation end products (AGEs) in foods, which are formed by Maillard reaction, has demonstrated as risk factors associated with many chronic diseases. The AGEs inhibitory activities of five common phenolic acids (protocatechuic acid, dihydroferulic acid, p-coumaric acid, p-hydroxybenzoic acid and salicylic acid) with d...
Questions
Questions (20)
Hi,
I am working with some genomics data and I need to predict some gene clusters in genomes. I want to use a different method given by Takeda et al., 2014. I am calculating but i am not confident about my results. Can someone help me with understanding the procedure of calculations?
Thank you very much,
Hi,
I am working on compound identification using HPLC. Each compound has its unique Uv-Vis spectrum. Here are some compounds that are creating trouble for me and I can’t recognize their spectrum. I am attaching these spectra. Please let me know if someone is familiar with those spectra. I’ll be highly obliged.
Thank you very much.
Hi, as pBR322 is one of the oldest plasmids used. Can pBR322 be used in the construction of a yeast plasmid or can a vector having pBR322 ORI and BOM be assembled in yeast?
Thank you for the answers.
Hello Every one, I am wondering whether southern blot can determine the mutated genes while they will be used as probes. Will it show result if the DNA fragment used as Probe has mismatches at intervals. Secondly, If the probe is 1KB long, but 700 bp is same, but 300 bp are not same on either of the sides. Will southern blot give the result or not? If yes, what is the possible solution to get a accurate band. Thank you.
Hello Every one,
I am looking for the fate of stop codon when it comes within the protein sequence, is it skipped or place a random amino acid? Shortly, Did anyone experienced double stop codon in mRNA?
Thanks for reply.
Hello Everyone, I want to convert some large STO files to FASTA, do anyone know any good server to do it, Thank you so much,
Hello Every one,
I want to search some homologous proteins from some database using HMMER but i am facing some problem, HMMER is being failed to search, can anyone help me to sort this problem out? Thank you, here are the pictures,
Hello Everyone, I need to go through a question. From the theoried and laws, all the organisms having 2n number have a pair of genes, named as Allels of each other, here are 3 questions. 1. Do both of them having same sequesce (probably not)? 2. If we mask the effect of 1 gene, by silencing or knockout or gene manipulation techniques, what will happen to its allel, will it work or not? 3. Do allels work independent of each other, work dependently or in cooperative manner?
Thanks for replies,
Hello,
I am working on some gene manipulation experiments; many times I faced a problem, When i t-clone my GOI(Gene of Interest) if give many colonies. When i run Colony PCR from the middle portion it gives exact sized positive result, but when I try to amplify full fragment, it gives nothing. When I mini-prep it and double digest it, I dont get any band of vector size nor fragment size. I did this about 10 times, What may be the problem? It is happened when I am using LA taq Takara.
Hello Mates, I need to know about HEPES concentration to make E. Coli competent cells . Previously, I am using 0.1 M CaCl2, but now i want to use HEPES only, Thanks for suggestions.
