Muhammad Nasir Khan Khattak

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Verified

Muhammad Nasir Khan verified their affiliation via an institutional email.

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• Ph.D
• Senior Researcher at Universität Greifswald

Owing to the stern importance of nutritional intakes for the growth and shaping life long events , we aim to elucidate the nutritional status of the schoolchildren according to the as definitions of the International Obesity Task Force (IOTF) and the Centres of Disease Control and Prevention (CDC) in 6 to 12 year-old schoolchildren from the rural d...

Stem cell treatment shows promise in treating conditions such as neurodegenerative disorders and spinal injuries, but its effectiveness is hampered by cell death and apoptosis. Improving the differentiation of MSCs into neural cells could enhance their therapeutic potential. The role of miR-22-3p in human dental pulp stem cells (HDPSCs), a superior...

Background The investigation of prevalence trends of metabolic cardiovascular risk factors is important for appropriate planning of future health programs aiming to prevent cardiovascular morbidity and mortality. In a previous study, we demonstrated an increase in the prevalence of type 2 diabetes (T2D) between 2000 and 2010 in Northeast Germany. T...

The study isolated gelatin from Labeo rohita scales and characterized it using Fourier transform infrared spectroscopy (FTIR), X-Ray diffraction (XRD), and scanning electron microscopy (SEM). The resulting gelatine and commercial bovine bone gelatin were found to have crystalline structures, with both being porous and spherical. L. rohita scales yi...

Background and aim: As the spleen plays a significant role in immunity, the aim was to investigate the associations of different body composition markers derived from various sources with spleen volume in a general population sample. Materials and methods: Cross-sectional data of 1095 individuals (570 women; 52%) aged between 30 and 90 years were c...

We report the proteomic profile of Epidermal Mucus (EM) from Labeo rohita and identified the differentially abundant proteins (DAPs) against Aeromonas hydrophila infection through label-free liquid chromatography-mass spectrometry (LC-MS/MS). Using discovery-based proteomics, a total of 2039 proteins were quantified in nontreated group and 1,328 pr...

This study reports the bactericidal activity of mucus extracts and biochemical characterization of skin mucus from five Cyprinids, including Labeo rohita, Ctenopharyngodon Idella, Gibelion catla, Hypophthalmichthys molitrix and Cirrhinus mrigala against ten different bacteria extracted from naturally infected fish. The bactericidal activity was mea...

Background Human dental pulp stem cells (HDPSCs) may be differentiated into neural lineages. The main aim of the study was to assess the DNA demethylation and histone deacetylation inhibition on the differentiation of HDPSCs into neural progenitor-like cells (NPCs). Methods HDPSCs were treated with 5-aza2′-deoxycytidine (AZA), DNA methylation inhi...

Background Both hormonal and genetic data reveal that the stress hormone cortisol and its regulating genes may affect the level of testosterone in humans. It is uncertain whether type 2 diabetes mellitus would manifest similarly. Furthermore, a genetic strategy to screen out the stress system genes that may contribute to testosterone decline in hum...

Background Differentiation of Immortalized Human Bone Marrow Mesenchymal Stromal Cells - hTERT (iMSC3) into adipocytes is in vitro model of obesity. In our earlier study, rosiglitazone enhanced adipogenesis particularly the brown adipogenesis of iMSC3. In this study, the transcriptomic profiles of iMSC3 derived adipocytes with and without rosiglita...

Diabetes mellitus (DM), an endocrine syndrome characterized by high blood glucose levels due to abrogated insulin activity. The existing treatments for DM have side effects and varying degrees of efficacy. Therefore, it is paramount that novel approaches be developed to enhance the management of DM. Therapeutic plants have been accredited as having...

Hot springs ecosystem is the most ancient continuously inhabited ecosystem on earth which harbors diverse thermophilic bacteria and archaea distributed worldwide. Life in extreme environments is very challenging so there is a great potential biological dark matter and their adaptation to harsh environments eventually producing thermostable enzymes...

Human dental pulp stem cells (HDPSCs) have great potential to be used in regenerative medicine. To use these stem cells effectively for this purpose, they should be grown in a 3D cell culture that mimics their natural niches instead of a 2D conventional cell culture. The aim of this study was to grow the HDPSCs in the 3D cell culture created by Tra...

Our previous study revealed that the treatment of 5-aza-2-deoxycytidine (5-aza) inhibited while treatment of suberoylanilide hydroxamic acid (SAHA) enhanced the adipogenic differentiation of MG-63 cells. In this study, we examined the transcriptomic profiles of the derived adipocyte-like cells from MG-63 cells in the presence of 5-aza (Treatment 1)...

The plants extracts are widely used in traditional medicines and hence considered a potential source for drug discovery. In this study, we assessed the phytochemical composition of Asplenium dalhousiae and Asplenium polypodioides in acetone extracts and checked its antiproliferative potential in MDA-MB-231 cells. We found that both plants are rich...

In this study, oil degrading bacteria discovered from fish living near the oil ports at Karachi in Pakistan were characterized. The bacteria isolated from skin, gills, and gut in fish could consume crude oil as a source of carbon and energy. Total 36 isolates were tested using Nutrient Agar (NA) and MSA media with different crude oil concentrations...

Objective MG-63 cells that have osteoblastic and adipogenic differentiation potential were evaluated for internalization, and adipogenic differentiation in the presence and absence of the covalently functionalized aryl gold nanoparticles (AuNPs-C 6 H 4 -4-COOH). Results Inductively coupled plasma, flow cytometry and confocal microscopy analyses co...

A hypothalamic neuropeptide, RF-amide related peptide-3 (RFRP-3), the mammalian ortholog of the avian gonadotropin inhibitory hormone (GnIH) has inhibitory signals for reproductive axis via G-protein coupled receptor 147 in mammals. Moreover, RFRP-3 has orexigenic action but the mechanism involved in energy homeostasis and glucose metabolism is not...

Globalization and environmental regulations force organizations and producers to adopt sustainability in every aspect of their business. The concept of sustainability in manufacturing is now achieving maturity; however, there is still an opportunity to explore sustainability in perishable food items. The growth of population worldwide increased the...

Ficus latex is rich in polyphenolic compounds and hence considered as an antioxidant and anti-proliferative. Many studies are available on Ficus carica (common fig) whereas Ficus salicifolia is less studied. F. salicifolia grows in a harsh dry environment, therefore its latex was selected in the current study along with the F. carica for their comp...

The Echinoccocus spp. infection results in cystic or alveolar echinococcosis causing billions of dollars loss to the international economy in respect of expensive medical treatments, permanent loss of entire or part of organ, and a substantial decrease in livestock productivity. Therefore, the study was designed with objectives to know existence of...

The use of Probiotics in aquaculture got an importance in the previous years. Hence, present study was carried out to evaluate the effects of the commercially available dietary probiotic (a consortium of Bacillus subtilis and Saccharomyces cerevisiae) on the growth, proximate body composition, digestive enzymes, hematological parameters and immune...

Humans living close to carnivore habitats sometimes develop a negative attitude towards them because of the damages they cause. Such an attitude has costs, both for wild carnivores and local communities. Human costs are mostly in the form of economic loss to property and infrastructure, damage to crops, and depredation on livestock. In northern Pak...

The heavy metals were studied in water, sediments, algae, and various tissues of Glyptosternon reticulatum and Cyprinus carpio from River Swat, Pakistan, using flame atomic absorption spectrophotometer. The Zn, Cu, Pb and Ni were higher in water at sewage site compared to upstream and downstream sites. In sediments, the Ni and Cd were not detected...

Unraveling molecular mechanisms that govern adipocyte formation will lead to a greater understanding of obesity and subsequent treatment. In the current study, we assessed the effects of miR-22-3p, a non-coding RNA, on adipocyte differentiation from telomerase-transformed Mesenchymal Stromal Cells (iMSC3). The transfection of iMSC3 with miR-22-3p s...

The date palm (Phoenix dactylifera L.) is an important fruit crop with significant pharmaceutical potential. Little data are available on comparative pharmaceutical importance of the date pits and we designed this study to assess the antitumorigenic effects of date palm pits extracts from different Emiratis varieties. We used MDA-MB-231 cells deriv...

Diabetes Mellitus (DM) is an endocrine disorder. Different types of nanoparticles are used as drug and insulin carriers. However, these nanoparticles also induce toxicity. Therefore, in the current study, we prepared GNPs, characterize it through SEM, XRD, and prepare its nanoinsulin (NI), administered dose dependently to the DM. The trace elements...

Diabetes mellitus is characterized by hyperglycemia is a leading cause of death in both developed and underdeveloped countries. Few therapies are in practice, but rapid and effective control is still lacking. In the current study, we aim to check the effect of Gold nanoinsulin on glucose level, blood complete picture (CP) and blood biochemistry in...

RRecombination-activating gene 2 (rag 2) allies with recombination-activating gene 1 (rag 1) and regulates the V(D)J recombination of immunoglobulin (Ig) and T-cell receptor (TCR) genes. Being a key player in the adaptive immune response of vertebrates, functional characterization of rag 2 from yellow catfish is beneficial for understanding the bio...

Plants produce and release a vast variety of secondary metabolites with diversified functions, and possess ecological, toxicological and biological effects that mimic the activities of synthetic chemicals. Coumarins extracted from bacteria, fungi and many edible plants are widely used for their antibacterial, antifungal, antiviral, anti-HIV and ant...

Obesity is a major risk for diabetes. Brown adipose tissue (BAT) mediates production of heat while white adipose tissue (WAT) function in the storage of fat. Roles of BAT in the treatment of obesity and related disorders warrants more investigation. Peroxisome proliferator activator receptor gamma (PPAR-γ) is the master regulator of both BAT and WA...

Background: Pregnant women are particularly vulnerable to iodine deficiency. Considering the rural-urban disparities in nutritional status in Pakistan, this study aimed to assess regional influences on nutritional iodine status of pregnant women in Pakistan. Methods: Data were collected from 1246 pregnant women in all trimesters of pregnancy who...

An epidemiological study was performed with the aim to elucidate the prevalence of gastrointestinal nematodes in equines of Bajaur and Mohmand Agencies in North-Western Pakistan from September 2013 to May 2014. The fecal samples from one thousand equines, comprising horses (n=83), donkeys (n=715), and mules (n=202) were collected and processed for...

The use of probiotics is considered effective for survival, growth and enhanced immune response in aquaculture. In the current study, effects of commercially available probiotic (Magic Plus) was investigated on survival, growth and immune response of Mori (Cirrhinus mrigala) in a polyculture system. The experiment was conducted for 90 days on 1200...

An unexplored soil microbial community associated with the root system of Nicotiana tabacum was isolated to analyze its impact on growth and yield of the crop. A total of nine isolates out of 180 were biochemically screened and characterized as potential plant growth-promoting rhizobacteria due to the expression of growth-promoting traits. All isol...

Cytochrome B (Cyt B) gene and control region of mitochondrial DNA are considered important for evaluating phylogenetic association of a species. In this study, we sequenced Cyt B and control region of Schizothorax plagiostomus and constructed phylogenetic association tree of S. plagiostomus with 23 schizothoracine fishes. We found S. plagiostomus t...

The present study was conducted to evaluate the impact of marble industry effluents on the accumulation of heavy metals in selected tissues of mahaseer (Tor putitora) in Barandu River. Fish samples were collected from three selected sites (upstream (US), effluents/industrial (IS), and downstream (DS) sites) on monthly basis for 8 months. Heavy meta...

Cyclophilin A (CypA), a peptidyl-prolyl cis-trans isomerase (PPIase), is a ubiquitous and multi-functional protein. In addition to its role as a host cell receptor for cyclosporine A, CypA has diverse functions in inflammatory conditions and diseases. CypA secreted in response to inflammatory stimuli binds to the cell surface via its receptor, CD14...

p>Present study was designed to investigate the effects of 200 mg/ml solvent/Kg of body weight of Bauhinia variegata’s leaf extract on neuromuscular coordination, locomotory and exploratory behavior of adult female albino mice. Six week old female albino mice were used as experimental animals. Mice were either orally supplemented with 200 mg/ml/Kg...

Toxoplasmosis causes huge morbidity and mortality in variety of warm blooded hosts including humans. The present study aimed at establishing the prevalence, distributions and risk factors of toxoplasmosis in the human population of Mardan district, northern Pakistan. A retrospective case-control study was launched and 600 subjects (cases= 301, cont...

Iodine deficiency remains a considerable challenge worldwide, even after decades of efforts to address the problem. The aim of this review is to present the current situation in historically iodine-deficient Pakistan regarding iodine nutritional status and place it in a global perspective. We collected relevant articles from online bibliographic da...

The complete mitochondrial genome is important for determining the phylogenetic relationship of species. In the current study, we sequenced the complete mitochondrial genome of Kunar Snow trout (Schizothorax labiatus). It was found to be 61,590 bp in length having 37 genes (13 protein coding genes, 2 ribosomal RNA (rRNA) genes, 22 transfer (tRNA) g...

Cyclophilin A (CypA), a well-recognized receptor for anti-inflammatory drug cyclosporine A (CsA) is a ubiquitous and multi-functional protein. Beside the diverse intracellular functions, CypA is secreted against inflammatory stimuli, where it activates and attracts leucocytes via CD147 to the stimulus site. Interestingly, it synergizes with other f...

Glutamine, one of the most important nutrients, plays a vital role in carbon metabolic pathway and has been reported to be required for the replication of several human DNA viruses. However, whether glutamine is required for RNA virus replication and the related mechanism remain elusive. Nervous necrosis virus (NNV), a positive-stranded RNA virus,...

Fish skin mucus is a dynamic barrier for invading pathogens with a variety of anti-microbial enzymes, including cyclophilin A (CypA), a multi-functional protein with peptidyl-prolyl cis/trans isomerase (PPIase) activity. Beside various other immunological functions, CypA induces leucocytes migration in vitro in teleost. In the current study, we hav...

Ladybird beetle is important group of beetles because they are important universal predatory and occupies important place in biological control. However, the ladybird beetle fauna of Pakistan is not extensively explored area wise. In this connection, the present study was conducted to explore the Ladybird beetle fauna of district Buner, Khyber Pakh...

Gut contents of 500 freshly slaughtered small ruminants (300 goats and 200 sheep) were studied using floatation and sedimentation methods followed by direct microscopic examination aims to investigate the prevalence of gastrointestinal tract helminthes in small ruminants of district Kohat, Khyber Pakhtunkhwa Pakistan during March-November 2013. Ove...

p>Patients with diabetes are considered to be at more risk of dyslipidemia and hypertension, hence targets for cardiovascular diseases. This study describes the possible role of Glycated Hemoglobin (HbA1c) and serum lipid profile as a biomarker in the detection of cardiovascular diseases. A cross-sectional study was carried out on 54 (33 males and...

Micro-RNAs are cellular components regulating gene expression at the post-transcription level. In the present study, artificial micro-RNAs were used to decrease the transcript level of two genes, AtExpA8 (encoding an expansin) and AHL25 (encoding an AT-hook motif nuclear localized protein) in Arabidopsis thaliana. The backbone of the Arabidopsis en...

Cyclophilin A (CypA) is a key member of immunophilins that has peptidyl-prolyl cis/trans isomerase (PPIase) activity. Besides acting as a cellular receptor for immunosuppressive drug cyclosporine A (CsA), CypA is involved in various cellular activities. CypA has an important role in viral infection which either facilitates or inhibits their replica...

The present study was conducted to investigate the effect of sub-lethal doses of Cypermethrin (CYP) on the survival and morphological and biochemical aspects of rohu (Labeo rohita) during early developmental stages. Newly fertilized eggs were incubated in the presence of sub-lethal concentration of CYP (20% of LC50 i.e., 8.43 mg L�1) in a well desi...

Tuberculosis (TB) is an ancient disease however, poses serious challenge in the modern era in developing as well as developed countries. Every year millions of peoples are infected with TB and millions of peoples lose their lives. This study was undertaken to determine the prevalence of pulmonary tuberculosis sputum smear positive (PTB-SS+ve) among...

MicroRNAs (miRNAs) play important roles in mediating multiple biological processes in eukaryotes and are being increasingly studied to evaluate their roles associated with cellular changes following viral infection. Snakehead fish Vesiculovirus (SHVV) has caused mass mortality in snakehead fish during the past few years. To identify specific miRNAs...

The present study was conducted to investigate the effect of sub-lethal doses of Cypermethrin (CYP) on the survival and morphological and biochemical aspects of rohu (Labeo rohita) during early developmental stages. Newly fertilized eggs were incubated in the presence of sub-lethal concentration of CYP (20% of LC50 i.e., 8.43 mg L�1) in a well desi...

In a recently published study we observed that effluents from marble industry affected physicochemical characteristics of River Barandu in District Buner, Pakistan. These changes in water quality due to marble effluents may affect fish community. The present study was therefore conducted to evaluate the impacts of marble industry effluents on fish...

Schizothorax plagiostomus, a fresh water fish, is an economically important fish of Pakistan. In this study, the complete mitochondrial DNA (mtDNA) sequence of S. plagiostomus was explored. The mitogenome length was found to be 16 563bp with 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and 2 non-coding regions: origin of light-strand replic...

The mitochondrial DNA sequence represents a mainstay of phylogenetics. It allows biologists to elucidate the evolutionary relationships among species. It also permits an examination of the relatedness of populations, hence got importance in the field of biology. The current study was designed to know the mitochondrial sequence of Schizothorax esoci...

Context. Pakistan is considered severely iodine deficient on the basis of available data. However this data is collected in patches with severe iodine deficiency. Objectives. The current study was conducted to know the prevalence of goiter and iodine nutrition status in the district Karak. Design. Thirty clusters approach was adopted for the study....

Paint is a liquor blend, used as a decorative or protective coating. Paints are the main source of volatile organic compounds (VOCs), very harmful for the environment and human beings. In the present study, fungal and bacterial growth on paint flakes sandwiched between the mineral salt medium agar layers were subjected to various analysis. Dry cell...

Grey and black francolins are the prime game birds of Pakistan. According to Red Data Book both species are declared as threatened worldwide. However in Pakistan the status of both species is not assessed. Therefore to study population and habitat of both the species is of critical importance. The current study was conducted in Mang game reserve, H...

Industries play an important role in improving the living standard but at the same time cause several environmental problems. Therefore, it is necessary to evaluate the impact of industries on the quality of environment. In the present study, the impact of marble industry effluents on water and sediment quality of Barandu River in Buner District, P...

Bats (Mammalia: Chiroptera) are significantly important as seeds dispersal, pollinators and biological pest control agents. The present study was conducted to explore the diversity of bats in districts Charsadda, Kohat, Mardan, Nowshera and Peshawar, Khyber Pakhtunkhwa (KP), Pakistan from July 2011 to May 2013. During the present study 10 bat speci...

Bats (Mammalia: Chiroptera) are significantly important as seeds dispersal, pollinators and biological pest control agents. The present study was conducted to explore the diversity of bats in districts Charsadda, Kohat, Mardan, Nowshera and Peshawar, Khyber Pakhtunkhwa (KP), Pakistan from July 2011 to May 2013. During the present study 10 bat speci...

Bats (Mammalia: Chiroptera) are significantly important as seeds dispersal, pollinators and biological pest control agents. The present study was conducted to explore the diversity of bats in districts Charsadda, Kohat, Mardan, Nowshera and Peshawar, Khyber Pakhtunkhwa (KP), Pakistan from July 2011 to May 2013. During the present study 10 bat speci...

The present study was conducted to investigate the contamination of water, sediments, and fish tissues with heavy metals in river Panjkora at Lower Dir, Khyber Pakhtunkhwa, Pakistan. Water, sediments, and fish (Shizothorax plagiostomus) samples were collected from September 2012 to January 2013 at three different sites (upstream site at Sharigut, s...

The present study was conducted to investigate the contamination of water, sediments, and fish tissues with heavy metals in river Panjkora at Lower Dir, Khyber Pakhtunkhwa, Pakistan. Water, sediments, and fish (Shizothorax plagiostomus) samples were collected from September 2012 to January 2013 at three different sites (upstream site at Sharigut, s...

their transitionary status which is due to the volatile law-and-order situation in this region from the last few decades. The present study aimed at elucidating the ABO and Rh blood groups polymorphisms in the Bajaur population, which is a war affected territory in the in north-western Pakistan. Blood groups data were obtained from 1,200 unrelated...

p class="NoSpacing">Pakistan is considered to be one of the most severely iodine deficient countries in the region. A decade earlier, 70% of the population was estimated to be at risk of iodine deficiency. However, the recent use of iodized salt has reduced the intensity of the problem. Earlier studies regarding iodine deficiency in Pakistan were r...

Context. Pakistan is considered to be severely iodine deficient with 70% population at risk of iodine deficiency. Objectives. This study was designed to determine the prevalence of goiter and status of iodine in district Charsadda, Pakistan. Design. The study was carried out using the 30 cluster approach. Subject and Methods. From each cluster, 40...

The present study was designed to detect the presence of tick-borne parasites (Theileria and Babesia spp.) in 196 blood samples collected from apparently healthy sheep and goats from two provinces, Punjab and Khyber Pukhtoon Khwa, in Pakistan. Reverse line blot (RLB) assay was applied for the parasitic detection by the amplification of hypervariabl...

Ectoparasites increase birds' morbidity by sucking blood and causing irritation to them hence affect their economic production. In the current study comparative prevalence of ectoparasites was studied in domesticated and wild black (Francolinus francolinus) and grey partridges (Francolinus pondicerianus) from Khyber Pakhtoonkhwa province of Pakista...

Hintergrund und Ziele: Der Wnt-Signalweg spielt eine Rolle bei der Kontrolle der Zellproliferation und Differenzierung in vielen Geweben, einschließlich des Hormonsystems. Dieser Signalweg wurde auch in verschiedenen Tumorarten nachgewiesen. Allerdings ist nur wenig über die Wnt-Signalkaskade in Hypophysenzellen und bei der Tumorgenese bekannt. Die...

Water pollution is one of the major threats to public health in Pakistan. Drinking water quality is poorly managed and monitored. Pakistan ranks at number 80 among 122 nations regarding drinking water quality. Drinking water sources, both surface and groundwater are contaminated with coliforms, toxic metals and pesticides throughout the country. Va...

The Wnt/ß-catenin signalling pathway is involved in tumorigenesis including endocrine tumors. We investigated the Wnt/ß-catenin pathway's modulation by corticotropin-releasing hormone (CRH) and somatostatin or somatotropin release-inhibiting factor (SRIF) in mouse pituitary AtT-20 corticotroph cells. The Wnt/β-catenin signalling pathway was activat...