Muhammad Muddassir Ali

• DOCTORATE
• Assistant Professor at University of Veterinary and Animal Sciences

Sulfoxaflor (SFX) is an insecticide that is commonly used for the control of sap‐feeding insects. Since SFX is extensively applied globally, it has been implicated in the substantial induction of environmental toxicity. Therefore, in this study, Allium cepa roots have been employed to elucidate the potential cytogenotoxic effects of SFX in non‐targ...

Bisphenol-A (BPA) is a predominately used chemical to manufacture polycarbonate plastic goods and as an epoxy resin to form protective linings. BPA can act as an environmental endocrine disruptor and can pass through the body of animals from different sources. The environmental factors include leakage from plastic tanks, water tubs, animal feed con...

The article illustrates the in vivo anthelmintic activity of seeds of Azadirachta indica and other synthetic drugs comparatively compare and to justify the said seed consumption as an anthelmintic by the customary therapists of animals in South-Asia. Crude aqueous extract (CAE) from seeds of A. indica at the dosage of 2 g/kg of body weight, levamis...

Aseel birds are known for their hardiness, thermotolerance, pugnacity, and robustness. However, they are highly susceptible to Avian Influenza (AI) and Newcastle Disease (ND) viruses which cause huge mortalities. Present study was designed to identify polymorphisms in exon-14 of Mx1, and 5’UTR & intron-7 of ROBO2 gene associated with antibody respo...

Recent Trends in Livestock Innovative Technologies explores the most recent developments and developing trends in the livestock farming industry. The book delves into the application of innovative technologies in various aspects of livestock production, management, and health through edited chapters. The book starts with an outline of the difficult...

Recent Trends in Livestock Innovative Technologies explores the most recent developments and developing trends in the livestock farming industry. The book delves into the application of innovative technologies in various aspects of livestock production, management, and health through edited chapters. The book starts with an outline of the difficult...

Recent Trends in Livestock Innovative Technologies explores the most recent developments and developing trends in the livestock farming industry. The book delves into the application of innovative technologies in various aspects of livestock production, management, and health through edited chapters. The book starts with an outline of the difficult...

Alternative and modified therapeutic approaches are key elements in culminating antibiotic resistance. To this end, an experimental trial was conducted to determine the cytotoxicity and antibacterial potential of composites of magnesium oxide (MgO) nanoparticles and antibiotics stabilized in sodium alginate gel against multi-drug-resistant Staphylo...

The COVID-19 pandemic, caused by the novel coronavirus SARS-CoV-2, has had a profound impact on various production sectors worldwide, including agriculture. Poultry production, a critical component of the global food supply chain, is also not spared from the effects of pandemic. Soybean, a versatile and widely consumed crop, plays a crucial role in...

Enhancing economical poultry production using low-cost animal blood plasma powder incorporated in poultry rations is a good step toward sustainable poultry production. The purpose of the present study was to evaluate the effect of dietary supplementation of animal blood plasma powder on production performance, dressed weight, and immune response of...

The COVID-19 pandemic, caused by the novel coronavirus SARS-CoV-2, has had a profound impact on various production sectors worldwide, including agriculture. Poultry production, a critical component of the global food supply chain, is also not spared from the effects of pandemic. Soybean, a versatile and widely consumed crop, plays a crucial role in...

Currently, aquatic and terrestrial ecosystems are continuously and chronically polluted by cocktails of countless chemical compounds. The susceptibility to infections is tremendously increasing in a variety of organisms due to exposure to environmental pollutants. Pendimethalin, an herbicide, is continuously used in agriculture to remove unwanted b...

Vancomycin-resistant Staphylococcus aureus (VRSA) is a zoonotic life-threatening pathogen. Vancomycin exhibits anti-bacterial activity by inhibiting peptidoglycan synthesis by binding to the D-ala-D-ala terminus of the peptidoglycan. But in VRSA, D-ala-D-ala is replaced by D-ala-D-lactate due to the presence of vanA, vanB or vanD genes. This study...

Background Methicillin-resistant Staphylococcus aureus (MRSA), affecting livestock and human beings, has become a global public health hazard with economic consequences. Aims The current study was designed to investigate the prevailing MRSA-associated subclinical mastitis and associated risk factors in dairy buffaloes. The study also highlighted t...

Background: Outbreaks of small ruminant morbillivirus (SRMV) are regularly occurring in Pakistan despite vaccine availability. This study was designed to identify substitutions within the immunogenic structural and functional regions of the nucleocapsid, fusion, and hemagglutinin genes of SRMV and their comparison with vaccinal strains of Nigerian...

The infection of Cryptosporidium in pigs causes digestive system ailments, diarrhea and weight loss serving as an economic burden, especially in newborn animals. The bacterial fermentation products of short-chain fatty acids have important roles in immune function, microbiota regulation, osmotic balance and metabolism. However, till now little know...

Intestinal parasites are of great economic importance in livestock. However, scarcity of data has been found about the prevalence of four important intestinal parasites including Cryptosporidium sp., Giardia duodenalis, Enterocytozoon bieneusi and Blastocystis sp. infection in free-ranged yaks and Tibetan pigs during the winter season on the platea...

Data regarding the therapeutic potential of Caladium lindenii (C. lindenii) are insufficient. It becomes more important to explore plants as an alternative or palliative therapeutics in deadly diseases around the globe. The current study was planned to explore C. lindenii for its anticancer activity of ethanolic and hexane extracts of C. lindenii l...

Staphylococcus aureus is emerging as a ubiquitous multidrug-resistant pathogen circulating among animals, humans, and their environment. The current study focused on molecular epidemiology and evidence-based treatment against S. aureus from bovine endometritis. For this study, n = 304 cattle were screened for endometritis using ultrasonography whil...

Background Hepatitis E virus (HEV) infects the liver and can cause the swelling of the liver. Swelling of the liver subsides after a few weeks of infection depending upon the severity of the disease. Hepatitis E virus (HEV) infection is endemic in Pakistan. Generally, four genotypes are prevalent worldwide. Methods The current study was conducted...

The current study was designed to characterize methicillin-resistant Staphylococcus aureus (MRSA) isolated from bovine milk, along with its response to antibiotics, and ultimately reverse its mechanism of resistance by modulation with non-antibiotics. The synergistic combination of antibiotics with NSAIDs were tested in-vivo by giving MRSA challeng...

Foot-and-mouth disease is responsible for severe economic losses to the livestock industry of Pakistan. This study aimed to use Swiss albino mice as a cost-effective experimental animal model to study different immunological and histopathological aspects of FMDV instead of natural targeted species like cattle. After isolation of field isolates FMDV...

Bisphenol S (BPS) is an analog of bisphenol A, which is used as substitute of BPA in many products like airport luggage tags, baby bottles, plastics, and epoxy resins etc. Bisphenol S can cause toxic effects in different organisms, i.e., mice, rat, zebrafish, and C.elegans, etc. Bisphenol S is also known as “endocrine disruptor” due to its ability...

Use of antibiotics without following standard guidelines is routine practice in developing countries which is giving rise to genetic divergence and increased drug resistance. The current study analyzed genetic divergence and drug resistance by S. aureus and therapeutic efficacy of novel antibiotic combinations. The study revealed that 42.30% (minim...

Livestock is an integral part of agriculture countries where ticks play significant role as potent pests causing considerable losses to economy and health. Drug resistance has made these pests supersede conventional therapies and control programs Nanotechnology here comes as an advancing and significant candidate alternatively able to reverse drug...

Avian pathogenic Escherichia coli (APEC) induces colibacillosis, an acute and systemic disease, resulting in substantial economic losses in the poultry sector. This study aimed to investigate the antibiotic resistance pattern associated with frequent virulence gene distribution in APEC O78:K80 that may cause pathological alterations in chickens. Th...

Staphylococcus aureus (S. aureus) has become a leading animal and public health pathogen that keeps on transferring from one host to other, giving rise to newer strains by genetic shifts. The current study was designed to investigate the epidemiology and genetic relatedness of mecA gene in S. aureus isolated from pets, immediate individuals in cont...

The present study aimed to evaluate the genotoxic potential of cerium oxide (CeO 2 ), magnesium oxide (MgO) nanoparticles and their ionic forms by alkaline comet assay. Eisenia hortensis were exposed to different series of concentrations (25, 50, 100, 200, and 400 μg/ml) of chemicals for 48 h to find LC 50 . The LC 50 for MgO and CeO 2 NPs were 70...

Nowadays, green synthesized nanoparticles (NPs) are extensively investigated to explore their biological potential. They are being explored to treat different infectious and cancerous diseases. Therefore, the current study was designed to evaluate the cytotoxic and genotoxic effects of biosynthesized silver nanoparticles (AgNPs) from the medicinal...

Pethoxamid is chloroacetamide herbicide. Pethoxamid is commonly used to kill different weeds in various crops. Pethoxamid can leach in the water and soil and can cause toxic effects to other non-target species. Current study is therefore aimed to perform the investigation of the cytotoxic and genotoxic effects of pethoxamid on Allium cepa cells.The...

Fluoride is commonly found in the soil and water environment and may act as chronic poison. A large amount of fluoride deposition causes serious harm to the ecological environment and human health. Mitochondrial dysfunction is a shared feature of fluorosis, and numerous studies reported this phenomenon in different model systems. More and more evid...

The potential spillover of foot and mouth disease (FMD) virus at the wildlife-livestock interface is mainly responsible for the outbreaks in captive wild ungulates. The current study was planned to investigate an FMD outbreak in the wild ungulate species in the Jallo Wildlife Park and breeding facility, Lahore, Pakistan from Mar 2021– Jun 2021. The...

Recently, there have been numerous reports showing that phthalates have negative human health impacts and may cause several diseases such as asthma, breast cancer, obesity, type II diabetes, and male infertility. Animals are also exposed to phthalates through the environment and can cause adverse health effects on them. Several studies have been fo...

Fungi can be used as a potent chemotherapeutic agent to treat various cancers. In current study acetone and methanol extracts of Terfezia claveryi, Terfezia boudieri, Terfezia olbiensis, Picoa lefebvrei, Picoa juniperi were used to assess total phenolic contents, antioxidant activity, ion-chelating impact, antimicrobial activity, the cytotoxic and...

Nevertheless, the discovery and increased production of pharmaceuticals has lead to substantial use of pharmaceutical products that include antibiotics and steroidal hormones. The day by day increase in human population across the globe is leading to the increased need of food production. Due to the excessive use of common antibiotics i.e. penicill...

The One Health (OH) institutions need dire attention of all the stakeholders i.e. legislative, administration, policymaker, corporate sector, professionals for animal welfare and human health, and environmental safety. A survey was conducted through questionnaire distribution among 1000 literate persons who could easily read English i.e. physicians...

Background: This study elucidated the molecular detection and pathological alterations in broiler chickens naturally infected with field circulating NDV strains along with their phylogenomic dynamics. Methods: Morbid tissue samples of diseased/dead chickens were collected from 100 poultry flocks presented to poultry disease diagnostic laboratories...

Staphylococcus aureus (S. aureus) has been a substantial economic problem due to its antibiotic resistance, persistence inside host and recurrence of disease. It escapes from immunity because of its intra-cellular growth. Moreover, it forms biofilm on both living and in-animate surfaces that leads to recurrent infections and growth in food industry...

The main aim of this study was to develop a set of functions that can analyze the genomic data with less time consumption and memory. Epi-gene is presented as a solution to large sequence file handling and computational time problems. It uses less time and less programming skills in order to work with a large number of genomes. In the current study...

Copper oxychloride gained great importance due to its broad-spectrum antifungal action to combat various fungal diseases of plants. However, excess quantity of cupric fungicides on plants causes enzymatic changes and toxic effects. Thus, the current study was aimed to investigate the cytotoxicity and genotoxicity of copper oxychloride on Allium cep...

Antibiotic-resistant bacteria have become the predominant etiology of endometritis and thus require effective treatment approaches. We used ultrasonography coupled with clinical signs and presented complaints of reproductive issues to investigate the epidemiology, phylogenetic analysis, antimicrobial resistance, and development of novel therapeutic...

The present study was designed to evaluate the effect of 2-methyl-2-nitro-3-guanidine on non-target mammalian species after acute exposure and its probable. Forty-five four weeks old Sprague Dawley rats of mixed sexes having mean body weight (80 ± 20g) were randomly divided into three groups fifteen each, namely C (control), E(exposed), and V (expo...

The present study describes the synthesis, characterization and antiochratoxin efficacy of zinc oxide nanoparticles against hepatotoxicity and nephrotoxicity in albino rats. Ochratoxin A (OTA) was produced by solid state fermentation on wheat grains using Aspergillus ochraceus and estimated by HPLC. ZnONPs were produced using leaf extract of Camell...

Outer membrane proteins (OMPs) of Aeromonas hydrophila have a variety of functional roles in virulence and pathogenesis and represent promising targets for vaccine development. The main objective of this study was to develop an in-silico model of beta-barrel OMP present among the valid A. hydrophila pangenomes (n = 22). With a program named the β-b...

Context Breeds of buffalo (Nili-Ravi and Kundhi) show different growth-rate and meat characteristics. Consumer preferences vary for the selection of meat according to the taste and sensory attributes. Buffalo meat is widely consumed in Asia and multiple buffalo breeds are reared for meat production. Aims The current experiment was designed to comp...

Background Prevalence of brucellosis and MLVA genotyping in animals and humans, isolated from different regions of Pakistan was performed. Animals having history of brucellosis from the field and local farms of Bannu, Mardan, Peshawar, Swat, Lahore and Islamabad were selected for blood collection. Humans that work with them were also selected for s...

Each year, foot-and-mouth disease leads to enormous economic losses to the livestock industry. Currently, the killed whole virus is widely using to control FMD. However, vaccination is constrained by lack of or incomplete protection. Therefore, along with vaccination, we need to find the antivirals against FMD. This study was conducted to investiga...

Bioinformatics education has been a hot topic in South Asia, and the interest in this education peaks with the start of the 21st century. The governments of South Asian countries had a systematic effort for bioinformatics. They developed the infrastructures to provide maximum facility to the scientific community to gain maximum output in this field...

A correction to this paper has been published: https://doi.org/10.1007/s13204-021-01793-9

Bovine tuberculosis (bTB) is a widespread zoonotic infection targeting the livestock sector, especially in developing countries, and posing a risk to humans and animal populations. Its recent prevalence in river buffaloes has been estimated as higher as 33.7%. In emergent countries like Pakistan, there is likeliness of human-livestock interfaces ex...

Ticks transmit a number of bacterial, protozoal and viral pathogens that cause many diseases like ehrlichiosis, hemorrhagic fever, theileriosis, babesiosis and anaplasmosis in livestock. This study was designed for molecular characterization of BM86 gene of Rhipicephalus (Boophilus) microplus tick. In this study, the BM86 gene was amplified, using...

Bovine mastitis is a host response to the microorganisms linked with the host immune system efficiency. Tumor necrosis factor-alpha (TNF-α) is a proinflammatory cytokine that plays a significant role in the innate and adaptive immune response. In this study, we characterized the upstream regulatory region and evaluated the relative mRNA expression...

A Correction to this paper has been published: https://doi.org/10.1007/s13353-021-00610-x

Tungsten oxide nanoparticles or nanopowder (WO3NPs) is commonly used in various industries and also in biomedical applications such as additives, pigments, and biomedical sensors. Non-judicious excessive use of these nanoparticles (NPs) could be a serious human health concern. Therefore, the current study aimed to explore the cytotoxic and genotoxi...

Green synthesis of nanoparticles (NPs) had shown the positive effects on various parameters of human health. However, this can vary depending on plant category, NPs nature, doses of green synthesized NPs and type of cell or tissue taken for experiment. The aim of this study is to evaluate the effects of Allium cepa (A. cepa)-mediated zinc oxide ZnO...

Myostatin is a protein translated by the the MSTN gene (also known as GDF8), is responsible for limiting muscle growth and strength. In thoroughbred horse (Equus Caballus), limited studies have been designed to examine the variants in the coding region of MSTN gene. However, no data is available regarding the single nucleotide polymorphisms (SNPs)...

Phyto-synthesized nanoparticles (NPs) having reduced chemical toxicity have been focused globally and become essential component of nanotechnology recently. We prepared green phytochemically (ginger and garlic) reduced NiO-NPs to replace synthetic bactericidal and catalytic agent in textile industry. NPs were characterized using ultra-violet visibl...

Emergence of resistance to antibiotics in bacterial pathogens and prevalence of residual effects of drugs pose serious challenges to public health worldwide. Diminishing efficacy of antibiotics calls for an expedited development of alternative antimicrobials. In this regard, zinc oxide (ZnO) nanoparticles (NPs) signify a latent and versatile inorga...

Anaplasmosis is caused by a gram negative obligate intracellular bacterium of the genus Anaplasma with the pathogen having zoonotic impact. The study aimed to estimate the prevalence of anaplasmosis in Pakistan, to unravel the association of potential risk factors and to investigate the effect on hematological parameters in affected small ruminants...

Silicon nanoparticles gained a great interest due to its use in biomedical research. It is considered as safe and has been used in nanomedicine. But literature still states its toxicity depending upon the size and dose of silicon nanoparticles. So, current study was aimed to evaluate the cytotoxicity and genotoxicity of silicon dioxide nanoparticle...

Endometritis is an inflammation of the uterus, which can reduce the reproductive performance in yaks. Salidroside is a natural monomer of Rhodiolarosea, it has anti-inflammatory, antioxidative and immune regulation properties. We conduct this study to investigate the effect of salidroside and dexamethasone on E. coli-induced inflammatory response o...

The current study was designed to characterize mitochondrial ATPase8/6 genes in Sindh ibex and to determin its phylogenetic relationship with other capra species. Blood samples were collected from Kirthar National Park, Sindh, Pakistan. PCR product was sequenced bi-directionally using dideoxy chain termination method after mitochondrial ATPase8/6 g...

Nickel (Ni) and cobalt (Co) doped ZnO nanoparticles were prepared using simple and cost-effective sol-gel technique. Effects of various weight ratios (5, 10, 15 and 20) % of Co and Ni-doped into ZnO have been examined. The prepared undoped and doped nanoparticles (NPs) were characterized using XRD, SEM, FTIR, EDX and UV-Vis spectroscopy. By analyzi...

Nanoparticles fabricated with biological reducing agents to minimize toxic effects of chemicals are being focused worldwide. Biologically synthesized metal oxide nanomaterials have become integral part of nanotechnology. The current work is providing an insight on ZnO nanoparticles having root extract of Z. officinale and A. sativum in terms of cat...

How to Cite This Article Abbas G, Nadeem A, Javed M, Ali MM, Aqeel M, Babar ME, Tahir MS, Tabassum S, Shehzad W: Mitochondrial cytochrome-b, cytochrome-c and d-loop region based phylogenetic and diversity analysis in blackbuck (Antilope cervicapra). Kafkas Univ Vet Fak Derg, 26 (1): 25-31, 2020. Abstract The present study was designed to find diver...

Background The hepatocellular carcinoma is a devastating disease and it’s a fifth common cancer. It has increased from the last ten years and the third commonest cause of cancer-related death. Many species of Thermopsis have been used as a source of traditional oriental medicines. Its many species are endemic in different regions of the world, for...

The aim of this work is to assess biomarker responses to stresses caused by pollution of the marine environment in the Gulf of Annaba, using the bivalve mollusc Donax trunculus and Mytilus galloprovincialis. Males and females were collected during the summer from site 1 considered to be far from pollution sources (SM1 and SD1), site 2 (SM2 and SD2)...

Brassinosteroids (BR) are the newly discovered plant hormone that protects the plants from biotic and abiotic stresses. Plants produce these hormones all the time, but the variation occurs in the quantity and location of its production. It is proved that brassinosteroids help the plants to regulate its response against stress conditions and make pl...

The current study was designed to evaluate genotoxicity of different sizes of iron oxide nanoparticles (IONPs) and ionic iron using coelomocytes of the earthworms Eisenia hortensis. Earthworms were exposed to different series of IONPs and ionic iron concentrations to find the respective LC50 of the chosen chemicals. LC50 for < 50, <100 nm and the i...

Tetramethylpyrazine (TMP) is a biologically active ingredient, which is isolated from a popularChinese medicinal plant. It has been used effectively to treat ischemic heart problems, cerebrovascular and thrombotic vascular diseases. This study was designed to evaluate the effect of TMP on calciumsensing receptors in pulmonary artery smooth muscle i...

Methicillin resistant Staph aureus (MRSA) is an emerging public health concern from dairy milk, and its diagnosis by phenotypic methodology is experiencing higher discrepancies. The present study was planned to estimate discrepancies in phenotypic identification of MRSA and MSSA (Methicillin sensitive Staph aureus) in relation to mecA, and prevalen...

Traditional medicines are used for therapeutic purposes all over the world. Many endemic plants all over the world have magical therapeutic potential. These could be explored further for medicinal purposes and hence can be preserved for their proper propagation. Thermposis turcica is endemic to Turkey. It’s general anti-oxidant and anti-cancerous a...

The bovine leptospirosis is an economically important zoonotic disease of flood affected areas worldwide, but scarce information is available about its epidemiology in Pakistan. This is a first study on sero-epidemiology of bovine leptospirosis in Pakistan. The objectives of this study were to investigate the sero-prevalence and associated risk fac...

Background: HCV has very diverse genotypes in nature, divided into variable number of subtypes. Due to epidemiologically divergent subtypes, it shows diverse geographical distributions, which makes it difficult to treat. This disease plays havoc with underdeveloped and developing countries due to scarcity of data and poor awareness among common peo...

This study was aimed to evaluate the status of total oxidant, antioxidant capacity and DNA damage caused by gunshot for wild falcons. Wounded and debilitated buzzards (buteo family) (n=20) were examined having different types of injuries caused by gunshot and other minor accidents. Buzzards were divided in to two groups, having gunshot wounds (n=10...

This study examined the aflatoxin levels in various dairy feed concentrates, silage, total mixed rations and different feed ingredients. Of the total 215 samples, 50 samples of dairy concentrate feed, roughages were i.e. Silage (n=23), TMR (n=9), Hay (n=8) and 115 samples of other feed ingredients were analyzed. After Extraction and filtration, afl...

This study aimed to evaluate the genetic resistance of Pakistani goat breeds (Beetal, Teddy, Angora, Nachi) against Haemonchus contortus. In total, 13 animals of each breed, irrespective of sex, were selected. Following artificial infection with 5000 L3 (third stage) larvae of Haemonchus contortus to each animal, susceptibility and resistance of ea...

Cobalt and its different compounds are extensively used worldwide and considered as possible environmental pollutant. Earthworms are useful model organism and its different species are used to monitor soil pollution. No study has been found to detect cobalt chloride (CoCl2) genotoxicity in earthworms. So, current study aimed to evaluate CoCl2 induc...

Streptococcus equi subsp. equi is highly contagious equine-specific disease characterized by abscessation of the neck and head lymph nodes. The prevalence and carrier potential of S. equi was investigated using 194 nasal swabs and 56 pus samples collected from clinically infected horses and examined by PCR. S. equi was detected in 122 (48.8%) horse...

To study the canine diabetes mellitus, 500 blood samples were collected and blood glucose level (mg/dl) was measured. Out of 500 samples, 16 (3.2%) were positive for diabetes mellitus. Out of 16, 11 (68.8%) were female and 5 (31.2%) were male dogs. Clinical signs like hyperglycemia (95% CL= 82.93-100), polyuria (95% CL= 56.99-95.00), polydypsia (95...