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September 2015 - September 2019
- During my Ph.D studies i tried to develop novel POCT bio analytical methods to detect various metabolites such as Uric Acid,melamine thrombine in various biological samples e.g blood, urine and milk
As an effective separation tool, free‐flow electrophoresis has not been used for purification of low‐abundance protein in complex sample matrix. Herein, lysozyme in complex egg white matrix was chosen as the model protein for demonstrating the purification of low‐content peptide via an FFE coupled with gel fitration chromatography (GFC). The crude...
Cannabis sativa (C. sativa) is a widespread medicinal plant used for humans and cattles infectious diseases. The current study is carried out to assess phytochemical, antimicrobial, antioxidant, α-amylase inhibition and cytotoxic activity of the Cannabis sativa plant. An appropriate amount of phytochemicals like tannins, flavonoids, lycopene, and β...
An aptamer-linked assay of target biomarker (e.g., thrombin) is facing the challenges of long- term run, complex performance and expensive instrument unfitting clinical diagnosis in resource-limited area. Herein, a facile chip electrophoresis titration (ET) model was proposed for rapid, portable and low-cost assay of thrombin via aptamer-linked mag...
A novel analytical protocol was developed for general quality screening of chicken meat based on isoelectric focusing (IEF) and protein extraction. To demonstrate the developed protocol, twenty‐four chickens were divided into three groups; each had eight chickens. The chickens in Group 1 were slaughtered by exsanguination, Group 2 asphyxiated in wa...
Assays of uric acid (UA) in human urine and blood are of great significance to clinic diagnoses of many diseases, such as gout, renal failure, and hyperuricemia. Portable and visual quantification method, however, has been rarely developed for POCT detection of UA in urine and blood samples. In this work, we developed the electrophoresis titration...
Background Organochalcogen compounds have attracted the interest of a multitude of studies for their promising Pharmacological and biological activities. The antioxidant activity and acute toxicity of an organoselenium compound, 1-(2-(2-(2-(1-aminoethyl)phenyl)diselanyl)phenyl)ethanamine (APDP) was determined in mice. Methods Mice were randomly di...
High portability and green are two of the most important objectives pursued by microfluidic techniques. However, there are still many challenges for design of portable and visual microfluidic method (e.g., chip electrophoresis) thanks of uses of cumbersome pump, power supply and detector. Herein a facile double inner standard plot (DISP) model of e...
Melamine was sometimes adulterated to dairy products for false protein content increase in developing countries. However, portable sensor has not been developed for on-spot determination of melamine in dairy products yet. Herein, a distance-based sensor was advanced for the quantification of melamine in dairy products based on chip electrophoretic...
In this paper, an optimized method based on gas chromatography/time-of-flight mass spectrometry (GC-TOFMS) platform has been developed for the analysis of gut microbial-host related co-metabolites in fecal samples. The optimization was performed with proportion of chloroform (C), methanol (M) and water (W) for the extraction of specific metabolic p...