Muhammad Salman Hameed- PhD
- PhD Student at Fujian Agriculture and Forestry University
Muhammad Salman Hameed
- PhD
- PhD Student at Fujian Agriculture and Forestry University
Working as a Rsearch Associate in China
About
54
Publications
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Introduction
I hold a PhD in Insect Molecular Ecology and Entomology from Fujian Agriculture and Forestry University (FAFU), where my research focused on RNA interference (RNAi)-based pest management strategies. My primary objective is to develop innovative, environmentally sustainable solutions for insect pest control by integrating molecular tools with ecological insights. I have a strong background in insect molecular biology, RNAi, functional genomics, and host-pest interactions.
Current institution
Publications
Publications (54)
Plants produce a diverse array of secondary metabolites, including saponins, which play significant roles in defense against herbivores. This review investigates the potential of saponins from dicotyledonous plants, particularly within the Fabaceae (Leguminosae) family—encompassing species such as alfalfa, grass peas, lupins, lentils, chickpeas, an...
Cabbage (Brassica oleracea) is a globally important crop but often suffers from damage caused by sucking insect pests and various diseases. This study explores the effectiveness of microbial protein elicitors, such as PeaT1, PeBL1, Hrip1, and PeBA1, in managing Cabbage aphids (Brevicoryne brassicae). It investigates how these elicitors affect phyto...
The study evaluates the efficacy of leufenuron, emamectin benzoate, and thiamethoxam against the Dusky Cotton Bug (Oxycarenus laetus Kirby) using residue-based bioassay methods. Key findings indicate that emamectin benzoate showed the highest efficacy with the lowest LC50 value, making it the most potent insecticide among those tested. Leufenuron a...
Crops face constant threats from insect pests, which can lead to sudden disasters and global famine. One of the most dangerous pests is the Asian citrus psyllid (ACP), which poses a significant threat to citrus plantations worldwide. Effective and adaptive management strategies to combat ACP are always in demand. Plant resistance (PR) is a key elem...
Metals are dangerous to both human health and the environment since they are poisonous elements with a density greater than 5 g cm-3. The abundant and common heavy metals contaminating Agricultural lands are lead (Pb), cadmium (Cd) and chromium (Cr). Different organic amendments have been used to degrade and immobilize the metals with different eff...
Maize stem borer (Chilo Partellus), poses a substantial threat to maize crops all over the world, causing damage that ranges from 26.7% to 80.4%. Its destructive impact includes killing the shoot in young plants, leading to a "dead heart, " and damaging the upper stem in older plants through boring activity. To combat this issue, a fi eld experimen...
In the current study, the role of cell division cycle-associated 8 (CDCA8) in colon adenocarcinoma (COAD) was analyzed through comprehensive expression and methylation analysis, genetic mutation inquiry, and prognostic assessment. Utilizing the UALCAN database, CDCA8 expression analysis revealed significant overexpression in carcinogenic cells comp...
Worldwide, the dengue virus (DENV) is a common cause of infections. The disease can range from a minor flu-like illness to a serious complex ailment that kills the sufferer. Both the morbidity and fatality rates associated with dengue fever (DF) are high in Pakistan and other Asian nations. Through a mosquito bite, the DENV is spread. DENV was isol...
Maximum demands of staple food led to maximum nutrient application to increase rice cropping production which may affect the yield and quality of rice crops under semiarid environmental conditions. A study was conducted to evaluate the effect of boron, zinc and silicon application on the productivity of direct seeded rice. The field experiment was...
Maximum demands of staple food led to maximum nutrient application to increase rice cropping production which may affect the yield and quality of rice crops under semiarid environmental conditions. A study was conducted to evaluate the effect of boron, zinc and silicon application on the productivity of direct seeded rice. The field experiment was...
COVID-19 now spreading in Pakistan, massive consequences to health and livelihoods are feared as lockdown impacted the food basket and triggered logistics and harvest crisis especially in Punjab and Sindh. Millions of farmers are at the edge of disaster due to shortage of means of transportation, absence of labor force and threat of COVID-19 transm...
The COVID-19 epidemic is causing an international health emergency attention and imposing financial damage to economies to lock down the countries in strict quarantine measures. Further the relationship between temperature and humidity with COVID-19 affected cities around the world justifies exceptional consideration. Geographical location 30-50 °N...
For Pakistan and as well as for world community COVID-19 is a huge tragedy. The defensive actions plan of Pakistan to contain COVID-19 pandemic includes self-isolation, home quarantine, social distancing and lockdown. The lockdown strategy adopted by the different provincial governments which involve non-functioning and closure of all the commerce...
COVID-19 now spreading in Pakistan, massive consequences to health and livelihoods are feared as lockdown impacted the food basket and triggered logistics and harvest crisis especially in Punjab and Sindh. Millions of farmers are at the edge of disaster due to shortage of means of transportation, absence of labor force and threat of COVID-19 transm...
For Pakistan and as well as for world community COVID-19 is a huge tragedy. The defensive actions plan of Pakistan to contain COVID-19 pandemic includes self-isolation, home quarantine, social distancing and lockdown. The lockdown strategy adopted by the different provincial governments which involve non-functioning and closure of all the commerce...
The start of (2020) is underway with the rise of novel coronavirus known as SARS-CoV-2 that triggering pneumonia-related respiratory syndrome all over the world. It is the third intensive pathogenic and contagious coronavirus after severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome coronavirus (MERS-CoV) a...
There is a significant risk to the public health in world and strong apprehensions in Pakistan due to emerging infectious disease known as Covid-19 which is characterized by a completely unique mode of pathogen. In this paper we reviewed the first 65 days trajectory of Covid-19 outbreak in Pakistan and its impact on over the coming days, weeks and...
COVID-19 now spreading in Pakistan, massive consequences to health and livelihoods are feared as lockdown impacted the food basket and triggered logistics and harvest crisis especially in Punjab and Sindh. Millions of farmers are at the edge of disaster due to shortage of means of transportation, absence of labor force and threat of COVID-19 transm...
There is a significant risk to the public health in world and strong apprehensions in Pakistan due to emerging infectious disease known as Covid-19 which is characterized by a completely unique mode of pathogen. In this paper we reviewed the first 65 days trajectory of Covid-19 outbreak in Pakistan and its impact on over the coming days, weeks and...
The COVID-19 epidemic is causing an international health emergency attention and imposing financial damage to economies to lock down the countries in strict quarantine measures. Further the relationship between temperature and humidity with COVID-19 affected cities around the world justifies exceptional consideration. Geographical location 30-50 o...
The start of (2020) is underway with the rise of novel coronavirus known as SARS-CoV-2 that triggering pneumonia-related respiratory syndrome all over the world. It is the third intensive pathogenic and contagious coronavirus after severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome coronavirus (MERS-CoV) a...
Every individual has specific immune system which is entirely different from other individuals. The available data on Covid-19 showed comprehensive regional disparity within the human immune system with consistency to their particular effecting reasons. States with high dengue endemicity (>1.5 million cases/year) are apparently not as much hit by C...
Every individual has specific immune system which is entirely different from other individuals. The available data on Covid-19 showed comprehensive regional disparity within the human immune system with consistency to their particular effecting reasons. States with high dengue endemicity (>1.5 million cases/year) are apparently not as much hit by C...
Argonaute2 (AGO2) is a core catalytic component of the RNA-induces silencing complex (RISC) that binds to small guide RNAs containing small interfering RNA (siRNA) and microRNA (miRNA). The guide RNA leads RISC to the complementary mRNA for gene suppression. We cloned the full length cDNA (2193 bp) of the Ago2 gene (PxAgo2) from diamondback moth (D...
This study addressed the issue of quality control which is appealing considerable attention currently to nontoxic and healthy production for human consumption. Excess uptake of nitrate in leafy vegetable is suspected to have carcinogenic effects when consumed in high quantity in a human diet. In this regard, we investigated a strategy to minimize n...
The diamondback moth (DBM), Plutella xylostella (Lepidoptera: Plutellidae) is a very destructive crucifer-specialized pest that has resulted in significant crop losses worldwide. DBM is well attracted to glucosinolates (which act as fingerprints and essential for herbivores in host plant recognition) containing crucifers such as wintercress, Barbar...
The diamondback moth (DBM), Plutella xylostella (Lepidoptera: Plutellidae) is a very destructive crucifer-specialized pest that has resulted in significant crop losses worldwide. DBM is well attracted to glucosinolates (which act as fingerprints and essential for herbivores in host plant recognition) containing crucifers such as wintercress, Barbar...
The diamondback moth (DBM), Plutella xylostella (Lepidoptera: Plutellidae) is a very destructive crucifer-specialized pest that has resulted in significant crop losses worldwide. DBM is well attracted to glucosinolates (which act as fingerprints and essential for herbivores in host plant recognition) containing crucifers such as wintercress, Barbar...
Neuropeptides are endogenous active substances that widely exist in multicellular biological nerve tissue and participate in the function of the nervous system, and most of them act on neuropeptide receptors. In insects, neuropeptides and their receptors play important roles in controlling a multitude of physiological processes. In this project, we...
Argonaute (Ago) protein family plays a key role in the RNA interference (RNAi) process in different insects including Lepidopteran. However, the role of Ago proteins in the RNAi pathway of Plutella xylostella is still unknown. We cloned an Argonaute3 gene in P. xylostella (PxAgo3) with the complete coding sequence of 2832 bp. The encoded protein ha...
Over the last century, the global climate has been changing rapidly. Numerous anthropogenic activities have caused an unprecedented increase in the concentrations of atmospheric CO2 with other gases which consequently resulted in increased temperature and altered precipitation. In agriculture ecosystem, insect population dynamics are regulated by t...
Different IGRs Concentrations against maize stem borer (Chilo partellus Swinhoe) in field condition of Faisalabad-Pakistan. Pure and Applied Biology. http://dx. Abstract The experiment was conducted at the Youngwala experimental area of the Department of Agri. Entomology, University of Agriculture, Faisalabad in a Randomized Complete Block Design (...
The main aim of the present investigation was to know about the importance of leaf miners and the damage percentage they cause the present study was proposed to identify the species responsible for damaging the vegetables and other alternative host plants. The research was conducted during the year 2011, to determine the damage percentage of vegeta...
Aedes albopictus play a vital role in spreading disease such as dengue fever. Now day's dengue virus had become a serious problem for human life in Pakistan. Aedes albopictus is one of vector of dengue virus. There is temperature dependent development of Aedesalbopictus population. Temperature fluctuation has great effect on developmental stages of...
Questions
Questions (7)
Does anyone know, what is the exect time or method for transfection dsRNA into sf9 cell line?. Actually, i constructed EGFP vector and i did transfect it into sf9 cell line then i checked it by comfocal microscope. So, i got transfection successfully after finding green color into sf9 cells. Now, I want to suppress this EGFP gene into cells. But here, i am little confuse, either should i transfect EGFP dsRNA with EGFP vector or not?. I just want to know the exect method. If anyone knows, Please share here. I will be thankful for this.
Best Regards,
Muhammad Salman
Actually, my cells plate have contain GFP vector already and target gene (Ago2) also exist in cells. Here, i want to treat cell plate with both dsRNA of GFP and target gene (Ago2) but i am little confuse. i should to transfect with both dsRNA together at a time or separately into cells and what is the best procedure for the use of dsRNA into cells? Any body have better ideas about this, Please guide me. i will be thankful for this kind action. Warm Regards, Muhammad Salman
Actually, i want to check the normal, overexpression and suppression of target gene (Ago2) in RNAi pathway of DBM cells line. For this purpose, i want to know the best way to construct the dsRNA of Ago2 and GFP protein. i have little information about dsRNA preparation. there are two ways, one is by using Megascript RNAi kit and other is by using plasmid. if plasmid way is good then please guide me about protocol. how i can construct dsRNA with plasmid. if any Professor have an expertise about this, please give positve reponse as soon as possible. i will be thankful for this kind action.
Best Regards,
Muhammad Salman
i did cut both my expression vector (PIZT/V5-HIS) and my target gene (Ago2) after adding restriction enzyme (Kpn I and EcoR I). after this, i need to do T4 DNA Ligation. In this way, i did add .5µl vector (conc. is 74ng), 1 µl Ago2 (conc. is 21), 1 µl T4 DNA ligase buffer, 1 µl T4 DNA ligase and 6.5µl dd H2O. afer mixing, kept this tube at 16 °C for whole night then transformed all solution in DH5 cells. after clone pcr, there is no positive results. I request you, if any expert knows about my problem. please guide me good suggestion as soon as possible. thanks
Hi Professors,
I want to do vector (PIZT/V5-HIS) cloning. i did transformation of vector (.5 µL) with 50µL E.coli cells and keep in ice for 30 minuts then add L.B liquid 500 µL without antibiotic (Zeocin) and keep at 37 temperature for one hour. After this i did add 50 µL antibiotic (zeocin) in L.B gel then cool the gel on petridish plate. i did spread 10µL transformed sample (Vector + Ecoli) on petridish plates then keep these petridish at 37 temperature for whole night. next day i did pick up positive clone and add one clone with 5000 µL L.B liquid (with Zeocin) into test tube then keep this test tube at 37 C temperature for whole night then i did extracted vector by using plasmid extraction mini kit. after this, i did gel run but no positive result. i did get band in gel run. here i am confuse may be vector did not insert in cells. if any professor have an idea about this please guide me as soon as possible. thanks,
Best Regards,
Muhammad Salman
Actually, i am doing this vector (PIZT/V5-His) cloning. in this way, i will use Zeocin antibiotic and mix with L.B gel but i am little confuse how much amount of Zeocin antibiotic will mix in L.B gel for getting Positive clone on petri dish. i have liquid form of Zeocin antibiotic and concentration is 100mg/ml. the amount of L.B gel have 100 ml in one flask. if any friend or professor knows about this please guide me as soon as possible. i will be thankful for this kind action
i want to prepare dsRNA of Ago2. any friend can tell me about this?
