Muhammad Ashraf

Muhammad Ashraf
University of Veterinary and Animal Sciences | UVAS · Department of Pharmacology and Toxicology

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734
Publications
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39,142
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Publications

Publications (734)
Article
Micro and macro-morphological features contribute to plants' tolerance to a variety of environmental pollutants. The contribution of such structural modifications in the phytoremediation potential of Diplachne fusca populations collected from five saline habitats were explored when treated with 100 to 400 mM NaCl for 75 days along with control. Str...
Article
Lipoxygenases (LOXs) are a group of enzymes that catalyze the oxidation of polyunsaturated fatty acids and initiate the biosynthesis of secondary metabolites that are involved to control inflammation. In search of new and more potent LOX inhibitors, a series of new 3-(5-(4-chlorophenyl)-4-(2-furylmethyl)-1,2,4-triazole hybrids was prepared and scre...
Article
Full-text available
Key message Overexploitation of Salvadoraoleoides for medicine and animal fodder along with significant habitat loss in its natural ranges is causing a continuous decline in natural populations. Although, the IUCN Red List categorizes it as data deficit, there are some reports indicating that its natural populations are vulnerable in many regions o...
Article
Elucidation of the structural and physiological adaptations developed against environmental determinants is a key to understand acclimation and capability to withstand cold in plants colonizing high altitudes. In the present study, two Sorghum species, S. arundinaceum and S. nitidum, were collected from three different elevations (800, 1800 and 280...
Article
Full-text available
Lipoxygenases (LOXs) are a class of enzymes that catalyze the production of pro-inflammatory mediators, such as leukotrienes and lipoxins, via an arachidonic acid cascade as soon as they are released from the membrane phospholipids after tissue injury. In continuation of our efforts in search for new LOX inhibitors, a series of chlorophenyl-furfury...
Article
Full-text available
Thiamin is a crucial vitamin with a vast variety of anti-oxidative and physiological roles in plants subjected to abiotic stresses. We examined the efficiency of foliar-applied thiamin (50 and 100 mM) on growth, yield quality and key-biochemical characteristics of two cultivars (FD1 and FD3) of cauliflower ( Brassica oleracea L.) under water-defici...
Chapter
Recent advances in understanding of the molecular mechanisms of development and growth of plants provide an opportunity to increase substantially crop production under normal or stressful conditions. Plant stress responses are protective mechanisms activated by any type of stress through various signaling pathways. Nitric oxide (NO) is a signaling...
Article
Full-text available
α-Glucosidase inhibition is considered as an effective strategy for the treatment of diabetes mellitus. Currently, three α-glucosidase inhibitors are being used as drugs; Acarbose, Voglibose and Miglitol. The side effects of these drugs are forcing researchers to search for new and effective molecules. In this research work, novel 1,2,3-benzotriazi...
Article
Full-text available
Kinnow (Citrus reticulata), a type of citrus fruit grown in Pakistan, is world-famous due to its unique taste. However, deficiency of nutrients, environmental stresses, and pests and disease attacks are causing low fruit productivity , juice quality, and fruit dropping. In this study, integrated management of hormones and nutrients was performed in...
Article
This study reports the synthesis of a series of ibuprofen derivatives, including thiosemicarbazides 4a–f, 1,3,4‐oxadiazoles 5a–f, 1,3,4‐thiadiazoles 6a–f, 1,2,4‐triazoles 7a–f, and their S‐alkylated derivatives 8a–d. All of the newly synthesized derivatives were analyzed using 1H NMR, 13C NMR spectroscopy, and high‐resolution mass spectra (electron...
Article
Full-text available
Fimbristylis complanata manifests several biochemical and anatomical modifications to mitigate salinity-induced ionic disturbance. Three populations from salt-affected aquatic habitats were evaluated to assess degree of salinity tolerance and ion homeostasis. The collection sites were HR-Rasool headworks, LR-Lillah-Khewra foothills and SH-Sahianwal...
Article
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Cotton (Gossypium hirsutum L.) productivity is decreasing alarmingly by salinity. For genetic analysis of cotton response to salinity 8 parental genotypes and their 16 F 1 crosses were evaluated in Line × Tester fashion under normal and salt stress 15 dSm −1. Data were recorded for plant height, number of bolls plant −1 , boll weight, lint weight,...
Article
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Salinity has drastic effects on plant growth and productivity and is one of the major factors responsible for crop yield losses throughout the agricultural soils of the world. The mechanisms of salinity tolerance in plants are regulated by a set of inherent multigenes and prevalent environmental factors, which bring about a myriad of metabolic chan...
Article
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The role of ionic excretions and hyper-accumulation of salts through alterations of structural and functional traits in five populations of Suaeda vera Forssk. ex J.F. Gmel., a halophytic salt-indicator species of saline environments, was explored. Differently adapted populations of S. vera exhibited specific structural and functional responses for...
Article
Full-text available
A series of 28 novel naproxen derivatives (4a-f, 5a-f, 6a-d, 7a-f, and 8a-f) have been designed, synthesized, and characterized. The synthesized derivatives were assessed as dual inhibitors for 15-lipoxygenase (LOX) and α-glucosidase enzymes and checked for cytotoxicity and ADME studies. The inhibitory potential of naproxen derivatives for 15- LOX...
Article
The present work summarizes the synthesis of new sulfonamides and their N-substituted derivatives as possible agents for treating Alzheimer’s disease. The synthesis was triggered by reaction of 1,4-benzodioxane-6- amine (1) with 4-bromobenzenesulfonyl chloride (2) in aqueous alkaline media to yield N-(2,3-dihydro-1,4- benzodioxin-6-yl)-4-bromobenze...
Article
Full-text available
Fullerenols are carbon nanoparticles that have been declared as free radical sponges. There is a need to take a prudent path toward its applications in various biological systems. For plants, polyhydroxy fullerenes can be beneficial nanozymes with the ability to scavenge all forms of reactive oxygen species produced within sub-cellular compartments...
Article
Lower levels of zinc in the food lead to Zn deficiency in the local population of Pakistan. The study aimed to increase the Zn fraction of an edible vegetable for which in-house fabrication of nano-Zn oxide crystals was performed using the sol–gel method. The synthesized nano-crystals exhibited ~ 25 nm diameter, and the XRD peaks indicated a single...
Article
Full-text available
Fungi, important for growth of plants in arid lands, are expected to be involved in novel biochemical activities during fungal–plant interactions. We isolated 150 fungi associated with rhizosphere and root endosphere of two perennial grasses, Cymbopogon jwarancusa and Panicum antidotale, from Cholistan desert. The isolates were screened for their i...
Article
The future challenges associated with the shortage of fossil fuels and their current environmental impacts intrigued the researchers to look for alternative ways of generating green energy. Solar-driven water splitting into oxygen and hydrogen is one of those advanced strategies. Researchers have studied various semiconductor materials to achieve p...
Article
In the present study, twenty-eight (n=28) longitudinally rear male goat kids were slaughtered at monthly intervals (n=4/group) till the 6th month of age; to examine the testicular development and establishment of spermatogenesis through histo-architecture along with mRNA expression of related genes. The results showed that seminiferous tubular diam...
Article
Full-text available
The adaptability potential of plants enables them to colonize diverse habitats along elevation gradients. Studying these adaptive traits and linking them to environmental attributes provide useful information to understand limitations imposed by elevation gradients on distribution of plant species. To meet these objectives, six most dominant perenn...
Article
The phytoextraction potential of turnip and comparative effectiveness of three different organic acids towards removal of lead (Pb) was investigated under field conditions. The 20 d old turnip seedlings were exposed to different Pb levels (0.0218, 2.42 and 4.83 mM Pb) spiked in the soil. After 10 d of Pb application, the soil was spiked with 2.4 mM...
Article
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Considering that antimicrobial resistance (AMR) is a global challenge, there is a dire need to assess the knowledge, attitude, and practice (KAP) of clinicians in AMR endemic countries. The current multicenter, cross-sectional study aimed at highlighting gaps in antimicrobial (AM) stewardship and AMR among practicing doctors working in public terti...
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The aim of the present study was to examine the diversity for heat tolerance in a set of 105 accessions using some key morpho�physiological attributes. The data was standardized by calculating percent change over control, and used for phonetic analysis and construction of dendograms based on distance matrix (all primary data is given as supplement...
Article
Full-text available
Naturally adapted populations of a leafy succulent desert halophyte Salsola imbricata were evaluated for growth patterns, and, structural and functional modifications, that ensures their success in highly salt-affected habitats. The populations were collected from five diverse habitats least saline (DWF-Derawar Fort), moderately saline (TWT-Trawayw...
Article
Full-text available
The influence of varying concentrations (0, 1, 3, 4, 5, 6, 8, and 10 % v/v) of neem (Azadirachta indica) leaf extract on drought (40 % field capacity) stressed quinoa (Chenopodium quinoa Willd.) plants was assessed. During the current study two cultivars of quinoa (V7 and V9) were used. Our study revealed that water stress adversely affects the fre...
Article
Full-text available
As global warming progresses, agriculture will likely be impacted enormously by the increasing heat stress (HS). Hence, future crops, especially in the southern Mediterranean regions, need thermotolerance to maintain global food security. In this regard, plant scientists are searching for solutions to tackle the yield-declining impacts of HS on cro...
Article
Full-text available
Background Coronavirus disease 2019 (COVID-19) caused by the novel coronavirus-infected millions globally. Despite a wide range of advised options for the treatment of COVID-19, a single strategy to tackle this pandemic remains elusive, thus far. That is why we are conducting a clinical trial to find out the efficacy of iodine complex to clear a vi...
Preprint
α-Glucosidase inhibition is considered as an effective strategy for the treatment of diabetes mellitus. Currently, three α-glucosidase inhibitors are being used as drugs; Acarbose, Voglibose and Miglitol. The side effects of these drugs are forcing researchers to search for new and effective molecules. In this research work, novel 1,2,3-benzotriazi...
Article
Naturally adapted populations of a leafy succulent desert halophyte Salsola imbricata were evaluated for growth patterns, and, structural and functional modifications, that ensures their success in highly salt-affected habitats. The populations were collected from five diverse habitats least saline (DWF-Derawar Fort), moderately saline (TWT-Trawayw...
Article
Full-text available
Naturally adapted populations of a leafy succulent desert halophyte Salsola imbricata were evaluated for growth patterns, and, structural and functional modifications, that ensures their success in highly salt-affected habitats. The populations were collected from five diverse habitats least saline (DWF-Derawar Fort), moderately saline (TWT-Trawayw...
Article
Two ecotypes of Cenchrus setigerus Vahl (birdwood grass) were collected from dry mountains surrounding hyper-saline Kalar Kahar Lake and Gatwala artificial forest plantation. The ramets of the two ecotypes were established in pots for 15 days and then subjected to 100% FC (control) or 75% and 50% FC (field capacity) levels. Growth attributes such a...
Article
Full-text available
Drought is one of the most important abiotic stress factors limiting maize production worldwide. The objective of this study was to investigate whether photoprotection of PSII was associated with the degree of drought tolerance and yield in three maize hybrids (30Y87, 31R88, P3939). To do this, three maize hybrids were subjected to three cycles of...
Article
Full-text available
Optimum water availability at different growth stages is one the major prerequisites of best growth and yield production of plants. Exogenous application of plant growth regulators considered effective for normal functioning of plants under water-deficit conditions. A study was conducted to examine the influence of exogenously applied L-methionine...
Article
Full-text available
Polysaccharides are beneficially used as drug carriers via prodrug formation and offer a mechanism for better effectiveness and delivery of the drug. The unique geometry of hydroxypropylcellulose (HPC), a polysaccharide, allows the attachment of drug molecules with a higher degree of substitution. Therefore, HPC-gemifloxacin conjugates, i.e., macro...
Article
Full-text available
Arsenic (As) being a toxic metalloid adversely afects plant growth and yield, as well as poses severe risks to human health. Hydrogen sulfde (H2S) has emerged a vital signaling molecule regulating key plant growth processes under stress conditions. However, till date little information is available regarding the role of H2S in mitigating As toxicit...
Article
Full-text available
Objectives The objective of the study is to measure the efficacy of ionic-iodine polymer complex [1] for clinical and radiological improvement in coronavirus disease 2019 (COVID-19) patients. Trial design The trial will be closed label, randomized and placebo-controlled with a 1:1:1:1 allocation ratio and superiority framework. Participants All P...
Article
Full-text available
Background Cotton ( Gossypium hirsutum L.) is grown for fiber and oil purposes in tropical and sub-tropical areas of the world. Pakistan is the 4th largest producer of cotton. It has a significant contribution in the GDP of Pakistan. Therefore, the present study was performed to assess the genetic variations and genetic diversity of yield and fiber...
Article
Full-text available
Objectives The study objective is to quantify the effectiveness of ivermectin (subcutaneous/oral IVM) in the presence or absence of zinc (Zn) for clinical and radiological improvement in coronavirus disease 2019 (COVID-19) patients with moderate severity. Trial design This quadruple-blinded, placebo-controlled randomized clinical trial will be a m...
Article
Full-text available
Objectives Considering the therapeutic potential of honey and Nigella sativa (HNS) in coronavirus disease 2019 (COVID-19) patients, the objective of the study is defined to evaluate the prophylactic role of HNS. Trial design The study is a randomized, placebo-controlled, adaptive clinical trial with parallel group design, superiority framework wit...
Article
Two ecotypes of Cenchrus setigerus Vahl (birdwood grass) were collected from dry mountains surrounding hyper-saline Kalar Kahar Lake and Gatwala artificial forest plantation. The ramets of the two ecotypes were established in pots for 15 days and then subjected to 100% FC (control) or 75 and 50% FC (field capacity) levels. Growth attributes such as...
Article
The main objective of the study was to assess if joint application of melatonin (MT, 0.1 mM) and salicylic acid (SA 0.5 mM) could improve tolerance of pepper plants to arsenic (As) as sodium hydrogen arsenate heptahydrate (0.05 mM). The imposition of arsenic stress led to accumulation of As in roots and leaves, and increased contents of leaf prolin...
Article
Full-text available
Now-a-days, plant-based extracts, as a cheap source of growth activators, are being widely used to treat plants grown under extreme climatic conditions. So, a trial was conducted to assess the response of two maize ( Zea mays L.) varieties, Sadaf (drought tolerant) and Sultan (drought sensitive) to foliar-applied sugar beet extract (SBE) under vary...
Article
Full-text available
A study was carried out to evaluate the effectiveness of sugar beet extract (SBE) and glycine betaine (GB) in mitigating the adverse effects of drought stress on two maize cultivars. Seeds (caryopses) of two maize cultivars, Sadaf (drought-tolerant) and Sultan (drought-sensitive) were sown in plastic pots. Plants were subjected to different (100%,...
Article
Full-text available
This study was planned to investigate effect of colchicine on humoral immune responses and immune organs in mice. Humoral immune responses were evaluated by performing haemagglutination assay, mice lethality test and Jerne hemolytic plaque formation. Colchicine treated groups were administered 40µg/kg, 80µg/kg and 160µg/kg.Data obtained was analyze...
Article
Fifteen ecologically distinct ecotypes of Bermuda grass Cynodon dactylon (L.) Pers. were evaluated for key factors involved in its widespread distribution in different environmental conditions. Ecotypes collected from saltmarshes showed better vegetative growth, accumulation of inorganic ions (Na⁺, K⁺ and Ca²⁺), compatible solutes (proline and glyc...
Article
Arsenic (As) is a highly toxic contaminant in the environment. Although both inorganic and organic types of arsenic exist in the environment, the most common inorganic forms of As that adversely affect plants are arsenite (As III) and arsenate (As V). Despite no evidence for As being essential for plant growth, exposure of roots to this element can...
Article
The main intent of the current research was to appraise if combined application of hydrogen sulfide (H2S, 0.2 mM) and silicon (Si 2.0 mM) could improve tolerance of tomato plants to arsenic (As as sodium hydrogen arsenate heptahydrate, 0.2 mM) stress. Plant growth, chlorophylls (Chl), PSII maximum efficiency (Fv/Fm), H2S concentration and L-cystein...
Article
Synthesis of heterocyclic compounds encompassing multiple functionalities and their biological screening is the most adapted strategy in the world for pharmacological evaluation of future drug candidates. The undertaken research was initiated by esterification 2-(1H-indol-3-yl)acetic acid (1) with catalytic amount of sulfuric acid in ethanol to eth...
Article
Full-text available
The role of morpho-anatomical adaptations of six Kyllinga brevifolia populations in successfully invading hyper-saline environments was investigated. Physiological and anatomical characteristics showed a high degree of plasticity indicating its adaptability potential to a variety of environmental conditions. The population from hyper-saline saltmar...
Article
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Authors' Contribution SAK designed and conducted the research trial. MA supervised the research work. DS and SI helped in data collection. SS and UA assisted in manuscript write-up. SA did the statistical analysis of data. FA and MSH reviewed the manuscript. Cirrhinus mrigala naturally breeds in rivers and streams during monsoon season but in ahatc...
Article
Full-text available
Authors' Contribution SAK designed and conducted the research trial. MA supervised the research work. DS and SI helped in data collection. SS and UA assisted in manuscript write-up. SA did the statistical analysis of data. FA and MSH reviewed the manuscript. Cirrhinus mrigala naturally breeds in rivers and streams during monsoon season but in ahatc...
Article
It was aimed to assess that up to what extent endogenous nitric oxide (NO) and its sources are involved in glutathione (GSH)-mediated tolerance of maize plants to cadmium (Cd) stress. The Cd-stressed maize plants were sprayed with or without GSH (1.0 mM) once every week for two weeks. Before initiating the stress treatment, the Cd-stressed plants s...
Article
Fifteen populations of Calotropis procera (Aiton) W.T. Aiton (milkweed) were collected from ecologically different regions of Punjab to evaluate anatomical traits that contribute to the ecological success of this plant in heterogeneous environmental regimes. Structural features relating to stems and leaves showed phenotypic variation. Xeromorphy wa...
Article
Full-text available
Fifteen populations of Calotropis procera (Aiton) W.T. Aiton (milk-weed) were collected from ecologically different regions of Punjab to evaluate anatomical traits that contribute to the ecological success of this plant in heterogeneous environmental regimes. Structural features relating to stems and leaves showed phenotypic variation. Xeromorphy w...
Article
The contribution of one major or a combination of several physiological processes in salt tolerance was assessed in three local varieties (Blacklong, Advanta-1103, and Dilpasand) of ridge gourd [Luffa acutangula (L.) Roxb.] at varying salt levels (0, 75, and 150 mM NaCl). Based on growth attributes, var. Dilpasand as salt-tolerant and var. Blacklon...
Article
Full-text available
Plants have developed a number of physiological, biochemical, and molecular strategies to overcome water deficit conditions. Arabidopsis vacuolar pyrophosphatase 1 (AVP1) can enhance stress tolerance in plants through ion homeostasis, redox balance, and photosynthetic activity. In the present study, three transgenic tobacco lines overexpressing AVP...
Article
Full-text available
The principal intent of the investigation was to examine the influence of joint application of methyl jasmonate (MeJA, 10 μM) and a nitric oxide–donor sodium nitroprusside (SNP, 100 μM) to wheat plants grown under cadmium (Cd as CdCl 2 , 100 μM) stress. Cd stress suppressed plant growth, chlorophylls ( Chl ), and PSII maximum efficiency ( F v / F m...
Article
Full-text available
Efficient selection for salt tolerance using the choice of physiological traits is of great importance. In the present study, seven ecotypes of Panicum antidotale (four from the Cholistan Desert and three from the non-Cholistani area) were evaluated after three weeks of salt stress. Results showed that shoot fresh biomass decreased by 74 and 83% re...
Article
In search for new anti-inflammatory agents that inhibit the enzymes of arachidonic acid pathway as the drug targets, the present article describes the screening of 1,3,4-oxadiazole analogues against lipoxygenase (LOX) enzyme. The work is based on the synthesis of new N-alkyl/aralky/aryl derivatives (6a-o) of 2-(4-phenyl-5-(1-phenylcarbamoylpiperidi...