Muhammad AnwarShenzhen University · College of Life Sciences
Muhammad Anwar
PhD Horticulture Biotechnology
• Molecular Biotechnology
• Biotechnological Techniques
• Plant Tissue Culture
• Plant Tissue Culture Techniques
• Trans
About
48
Publications
10,622
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412
Citations
Citations since 2017
Introduction
Dr.Muhammad Anwar currently works at Shenzhen university, Shenzhen . Muhammad does research in Horticulture, Biotechnology and Bioinformatics. Their current project is 'MYB transcriptional factor'. Transformation, Gene expression,Tissue culture,Bioinformatics.
Additional affiliations
January 2004 - June 2018
Fujian Agriculture and Forestry University FuzhouChina
Position
- Researcher
Description
- PhD Scholar
Education
September 2014 - June 2018
July 2006 - December 2008
September 2002 - July 2006
Publications
Publications (48)
Microbial life is truly an important system for other living organism on the Earth. The tiny organisms are of great interest in research and academia for their better use to human life and to treat some diseases which came into due to their existence. From the past few decades, recent development in advanced microbiological resources and meta-genom...
Genetic manipulation of Haematococcus pluvialis is difficult because of the lack of a stable and convenient transformation system. The pH124-EGFP-Ble vector containing ble as a selective gene and EGFP as a reporter gene was constructed and employed for effective transformation. H. pluvialis protoplasts were obtained by treating with cellulase and m...
Plant and fruit surfaces are covered with cuticle wax and provide a protective barrier against biotic and abiotic stresses. Cuticle wax consists of very-long-chain fatty acids (VLCFAs) and their derivatives. β-Ketoacyl-CoA synthase (KCS) is a key enzyme in the synthesis of VLCFAs and provides a precursor for the synthesis of cuticle wax, but the KC...
Insects change their stimulus-response through the perception of associating these stimuli with important survival events such as rewards, threats, and mates. Insects develop strong associations and relate them to their experiences through several behavioral procedures. Among the insects, Apis species, Apis mellifera ligustica are known for their o...
Crop production is a serious challenge to provide food for the 10 billion individuals forecasted to live across the globe in 2050. The scientists’ emphasize establishing an equilibrium among diversity and quality of crops by enhancing yield to fulfill the increasing demand for food supply sustainably. The exploitation of genetic resources using gen...
Mytilin is one of the most important CS-αβ peptides involved in innate immune response in Mytilidae. In this study, we successfully identified four mytilin-like antimicrobial peptides (pernalins) from Asian green mussel Perna viridis by aligning the P. viridis transcriptome with 186 mytilins and myticins related sequences collected from the transcr...
Unicellular volvocalean green algal Haematococcus pluvialis, known as astaxanthin rich microalgae, transforms into aplanospore stage from the flagellate stage when exposed to the stress environments. However, the mechanism of the formation of aplanospore cell wall, which hinders the extraction of astaxanthin and the genetic manipulation is still un...
The MYB transcription factors (TFs) are evolving as critical role in the regulation of the phenylpropanoid and tanshinones biosynthetic pathway. MYB TFs relate to a very important gene family, which are involved in the regulation of primary and secondary metabolisms, terpenoids, bioactive compounds, plant defense against various stresses and cell m...
Climate change could be an existential threat to many crops. Drought and heat stress are becoming harder for cultivated crops. Cotton in Pakistan is grown under high temperature and low moisture, could be used as a source of heat and drought tolerance. Therefore, the study was conducted to Morphological, physiological and molecular characterization...
Chemical plant ecology is a multi-faceted field focusing on prevalence, task, and combination of plant-based secondary metabolites. Understanding of these secondary metabolites helps in the discovery of the applications of these secondary metabolites in medical science. Abiotic components of the ecosystem can be influenced by chemicals released by...
Pepper's (Capsicum annum) response to bacterial pathogen Ralstonia solanacearm inoculation (RSI) and abiotic stresses is known to be synchronized by transcriptional network; however, related molecular mechanisms need extensive experimentation. We identified and characterized functions of CabHLH113 -a basic helix-loop-helix transcription factor-in p...
Human completely rely on food and nutrition supply to absorb enough lutein, which is an important antioxidant beneficial to health of eyes and heart. In plants, lycopene epsilon-cyclase (LCYE) is the key enzyme in lutein synthesis pathway, where lycopene is converted into δ-carotene, α-carotene and lutein. In this study, for the first time, we succ...
Pepper's (Capsicum annum) response to bacterial pathogen Ralstonia solanacearm inoculation (RSI) and abiotic stresses is known to be synchronized by transcriptional network; however, related molecular mechanisms need extensive experimentation. We identified and characterized functions of CabHLH113-a basic helix-loop-helix transcription factor-in pe...
Plants, due to their sessile nature, have an innate immune system that helps them to defend against different pathogen infections. The defense response of plants is composed of a highly regulated and complex molecular network, involving the extensive reprogramming of gene expression during the presence of pathogenic molecular signatures. Plants att...
Background:
The yield of microalgae biomass is the key to affect the accumulation of fatty acids. A few microalgae can assimilate organic carbon to improve biomass yield. In mixotrophic cultivation, microalgae can use organic carbon source and light energy simultaneously. The preference of the main energy source by microalgae determines the biomas...
Short tandem target mimic (STTM) RNAs contain two non-cleavable microRNA (miRNA) binding sites linked by a spacer and can be used to effectively block the functions of specific miRNAs in animals and plants. However, the application of STTMs in unicellular organisms has not been reported. In this study, we tested different STTMs targeting miRNAs of...
This study was carried out in the laboratory of Shangqiu Institute of Technology, Henan to investigate the effect of a different combination of inorganic salt on the quality and physiological characteristics of cut flowers (CFs) of Perpetual Carnation. Furthermore, to find out the best preservation solution of inorganic salt that can enhance the or...
Background: The yield of microalgae biomass is the key to affect the accumulation of fatty acids. A few of microalgae can assimilate organic carbon to improve biomass yield. In mixotrophic cultivation, organic carbon source and light energy exist simultaneously. The preference of the main energy source by microalgae determines the biomass yield. Au...
Background: The yield of microalgae biomass is the key to affect the accumulation of fatty acids. A few microalgae can assimilate organic carbon to improve biomass yield. In mixotrophic cultivation, microalgae can use organic carbon source and light energy simultaneously. The preference of the main energy source by microalgae determines the biomass...
Different types of molecular approaches have been used for improving resistance against pathogens to secure food. Efficient and advanced genome editing tool as paralleled to earlier techniques like Zinc Finger Nuclease (ZFN), transcription activator-like effector nucleases (TALENs), and Clustered Regularly Interspaced Short Palin-dromic Repeats (CR...
Different types of molecular approaches have been used for improving resistance against pathogens to securefood. Efficient and advanced genome editing tool as paralleled to earlier techniques like Zinc Finger Nuclease(ZFN), transcription activator-like effector nucleases (TALENs), and Clustered Regularly Interspaced Short Palin-dromic Repeats (CRIS...
R2R3-MYB transcription factors play important roles in the regulation of plant flavonoid metabolites. In the current study, NtMYB3, a novel R2R3-MYB transcriptional factor isolated from Chinese narcissus (Narcissus tazetta L. var. chinensis), was functionally characterized. Phylogenetic analysis indicated that NtMYB3 belongs to the AtMYB4-like clad...
The WRKY transcription factors (TFs) family constitutes a major group of TFs in spermatophytes. Different studies have endorsed the considerable biological roles performed by WRKY TFs in plant growth, biotic and abiotic stress responses. Genomic and transcriptomic profiling facilitate us in understanding the WRKY genes in various plants and reveal...
Chinese narcissus (Narcissus tazetta L. var. chinensis Roem.) is a popular flower in Asia. However, flower colors are limited with all cultivars having a white perianth and yellow corona. Previous studies have shown no anthocyanin accumulation in this species. The reason for an absence of anthocyanins remains unknown, with the flavonoid biosyntheti...
A R2R3-MYB-like gene，named NtMYB5，was selected from transcriptome of Narcissus tazetta var. chinensis and was cloned in order to study its function in flavonoid metabolic pathway. Multiple alignments showed that NtMYB5 contained R2-domain and R3-domain. A pdLNLD/ELxiG/S motif was also found near the C-terminal. Phylogenetic tree analysis indicated...
R2R3 MYB transcription factors play key functions in the regulation of secondary metabolites. In the present study, a R2R3 MYB transcriptional factorNtMYB2was identified from Chinese narcissus (Narcissus tazettaL. var.ChinensisRoem) and functionally characterized.NtMYB2belongs to subgroup 4 of the R2R3 MYB transcription factor family that are relat...
The regeneration frequency of okra (Abelmoschus esculentus) is greatly
influenced by its genetic makeup and recalcitrant nature. Phenolic secretion, in
particular, is a major problem in okra tissue culture. This study describes a reproducible,
rapid, and more efficient in vitro regeneration method using cotyledonary node explants
of okra. Explants...
The regeneration frequency of okra (Abelmoschus esculentus) is greatly influenced by its genetic makeup and recalcitrant nature. Phenolic secretion, in particular, is a major problem in okra tissue culture. This study describes a reproducible, rapid, and more efficient in vitro regeneration method using cotyledonary node explants of okra. Explants...
Rice grassy stunt virus (RGSV) a member of Tenuivirus family, is very potent and destructive which effects rice crop in many countries, particularly China. Non coding RNAs have important functions in development and epigenetic regulation of gene expression in numerous organsisms. There is three type of small non coding RNAs have been found in eukar...
This study was aimed to evaluate the performance of different cotton genotypes at Chishtian District, Bahawalnagar region. Four cotton genotypes (IUB-2013, FH-142, FH-LALAZAR and MNH-886) were sown under two different sowing times i.e., normal sowing S1 (20 th April) and late sowing S2 (20 th May 2016). The experiment design was split plot design w...
The present study was planned to evaluate the transgenic potato plants containing chitinase (RCG-3) gene transformed through Agro bacterium for resistance against Rhizoctonia Solani and Fusarium solani. In vitro, plants were cultured for multiplication and then pathogenicity of transgenic potato plants harboring chitinase gene were carried out with...
Bioinformatics plays an important role in agriculture science. As the data amount grows exponentially, there is a parallel growth in tools and methods demand in visualization, integration, analysis, prediction and management of data. At the same time, many researchers in the field of plant sciences are unfamiliar with available methods, databases,...
A field experiment was carried out at Horticulture research area of Pir Meher Ali Shah-Arid Agriculture University Rawalpindi during, 2012 to study the effect of pre-harvest treatment of salicylic acid (0.1, 0.5 and 0.1 mM/L) on growth, yield and vase. The results showed that foliar application of SA at the concentration of 1.0 mM/L significantly i...
This study was designed to assess the influence of different soilless and soil-based media on the growth, yield and flower quality of Tegetes erecta (Marigold) under controlled conditions. Seeds were sown in seedling trays during winter of 2012. Seedlings were planted in pots, each containing different undertaken media, singly or in combination in...
Potato is a very important food crop and is adversely affected by fungus. Agrobacterium-mediated
transformation can play an important role in the improvement of potato. The present study was
conducted to optimize the different factors affecting Agrobacterium-mediated transformation of
chitinase gene. Nodes were used as explant of potato cultivar...
Cellulase is an important industrial enzyme which can be obtained from cheap agrowastes. Pakistan is an agriculture country, producing tons of waste in the form of wheat straw, rice bran, sugarcane bagasee, corn cobs, corn stover etc. The aim of the present study was to produce cellulase by using abundant agrowastes like corn cobs. The conditions w...
Questions
Questions (10)
I want to transform in Chlamydomonas reinhardtii. Please guide me on which is the best strategy for transformation, Linear or circular vector. what is the difference between Linear and circular vectors?
I need help. I want to construct a single vector with 10 genes about 14 KB for overexpression in Chlamydomonas reinhardtii. which strategies is best to construct this vector. is there any solution?
Please, I need help on how to calculate the flavonol?
the data is attached
L-1=
L-2=
Wild=
The value of 2-△△Cq is UFGT gene
A=2.63
B=1
C=1.12
Control=179
I want to ask how to calculate the fold change between Control and experimental treatments(A,B) .
Why the expression level of UFGT gene(in A and B) is the lowest as compared to control.
Please guide me
I need the protocol for the extraction of Anthocyanin, Proanthocyanin, Flavonol and total phenolic.
Please I need information.How to prepare the Agroinfiltration buffer solution?
I want to design the primer in order to obtained Full-length cDNA. Please recommend me the most reliable and authentic software for primer design. I will be special thankful to you.
I have also attached my sequence. Please help me to design two forward specific gene prime for RACE 3.
I have partial cds sequence of my gene having length 769 bp.i have design two forward primers at 5' end one from 17 sequence and 2nd forward from 556 sequence. I have used UPM(S) universal primer as Reverse primer at 3' end. I have obtained 1.4 KB . Then I ligated with Pmd@18-T Vector .Then I do the transformation in E.Coli. Then Pick the single colony and do PCR. the result show same band about 1.5 KB. when i send to the company for a sequence. there is no my gene sequence. Please guide me about it. what is the matter?
I have gene from MYB family. This gene has partial cds sequence is 769. I need its full-length cDNA. please guide me about it how can i get full sequence?
Projects
Project (1)
