Morahem Ashengroph

University of Kurdistan | UoK · Department of Biological Science and Biotechnology

The present study has been conducted towards isolation of moderately halophilic bacteria capable of transforming caffeine into theobromine. A total of 45 caffeine-degrading moderate halophiles were enriched from hypersaline lakes and examined for the biotransformation of caffeine to theobromine by thin-layer chromatography (TLC) and high-performanc...

To screen strains of halotolerant or halophile bacteria which are able to convert isoeugenol to vanillin, 36 different strains of bacteria isolated from the salty environments in Iran were investigated. During growth on isoeugenol, a moderately halotolerant Gram-negative coccobacil showed capability of converting isoeugenol to vanillin. Based on mo...

Abstract The popular demand for natural food additives has resulted in a number of processes for producing natural vanillin. Although there are chemical procedures and plant sources for vanillin production, microbial bioconversions are being sought as a suitable ‘natural’ alternative. The present paper describes the conversion of isoeugenol to vani...

There is a growing interest in exploiting microbial bioconversions for the production of high value-added products such as vanillin and vanillic acid. We conducted this study to screen strains of bacteria which are able to degrade ferulic acid, and to evaluate the possibility of forming vanillic acid via conversion of ferulic acid under resting cel...

Introduction: Nanoparticles (NPs) are of great interest in the design of various drugs due to their high surface-to-volume ratio, which result from their unique physicochemical properties. Because of the importance of examining the interactions between newly designed particles with different targets in the case of various diseases, techniques for e...

Background: According to gas chromatography - mass spectrometry (GC-MS) results of a previous study, six metabolites including alpha-terpineol, geranyl acetate, linalool, myrcenol, terpinolene, and thymol showed significantly more amount relative to other metabolites. Methods: A continuation of the previous study, the interaction of these metabo...

Background: Chalcogenide nanoparticles (NPs), such as silver selenide NPs (Ag2Se NPs), are used as quantum dots in pharmaceutical formulations for the detection of cancer cells, as well as in solar cells, sensors, and electrical, optical, and magnetic instruments. Objectives: The physical-chemical production of metal NPs is expensive and difficult...

Background: Copper carbonate nanoparticles have several applications in the fields of pigments, insecticides, and fungicides. They are also used as catalysts in chemical processes and crude oil desulfurization. Fungi can biosynthesize metal nanoparticles due to their high tolerance, extracellular synthesis, simplicity of extraction, and large-scale...

Introduction: It has been more than two years since the outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Wuhan, China. The SARS-CoV-2 has created an unprecedented pandemic (COVID-19) in the modern era and challenged all the scientific, economic, and social structures of the world. Extensive worldwide research has been con...

This study reports the biosynthesis of silver nanoparticles (AgNPs) using a Pseudomonas canadensis Ma1 strain isolated from wild-growing mushrooms. Freshly prepared cells of P. canadensis Ma1 incubated at 26–28 °C with a silver nitrate solution changed to a yellowish brown color, indicating the formation of AgNPs, which was confirmed by UV–Vis spec...

Introduction: The inactivation and eradication of multidrug-resistant bacteria, fungi, and viruses by conventional antibiotics and drugs have not been effective. The hindering of these pathogens in hospital-acquired infections caused by Gram-positive bacteria, particularly strains of S. aureus including community-acquired methicillin-resistant (CA...

Quantum dots (QDs) are semiconductor materials that range from 2 to 10 nanometers. These nanomaterials (NMs) are smaller and have more unique properties compared to conventional nanoparticles (NPs). One of the unique properties of QDs is their special optoelectronic properties, making it possible to apply these NMs in bioimaging. Different size and...

Severe acute respiratory syndrome (SARS)-CoV-2 from the family Coronaviridae is the cause of the outbreak of severe pneumonia, known as coronavirus disease 2019 (COVID-19), which was first recognized in 2019. Various potential antiviral drugs have been presented to hinder SARS-CoV-2 or treat COVID-19 disease. Side effects of these drugs are among t...

In this study, three nonpathogenic endophytic bacterial isolates recovered from Fragaria × ananassa stolon and petal and their antagonistic activity was evaluated against Colletotrichum nymphaeae, the causal agent of strawberry anthracnose under in vitro, in vivo, and greenhouse conditions. Bacterial isolates were identified as Bacillus spp. using...

Bean root rot (Fusarium solani) is an important disease in major areas of bean production in the world, including Iran, which causes significant damage to bean crops every year. To date, various methods have been used to control this fungus, including physical and chemical controls; however, due to the multiplicity of host and soil fungus, the use...

An antagonistic yeast strain was isolated from the strawberry fruit cv. Paros, and its antifungal properties against Botrytis cinerea causal agent of strawberry grey mold disease were evaluated under in vitro and in vivo experiments. The isolate was tentatively identified as Tetrapisispora sp. strain 111A-NL1 based on phenotypic characteristics and...

About 80% higher risk of amputation resulted from microbial infection was indicated for patients with diabetic foot ulcers (DFUs). Micro and nano-scaffolds made of natural polymers specifically cellulose, chitosan, and collagen can donate the biocompatibility, biodegradability, and bioavailability properties appropriate to accelerate wound closure...

An aquatic yeast capable of amorphous selenium nanoparticles (SeNPs) synthesis was isolated and characterized as a novel strain of Rhodotorula mucilaginosa R-8441 based on its morphology and amplification of ITS1-5.8 S ITS2 16 rDNA sequence. The effect of various parameters such as precursor concentration, biomass concentration, and time incubation...

Strawberry anthracnose, caused by Colletotrichum nymphaeae is an important disease of strawberry. In this study, an endophytic bacterium isolated from the stem of Fragaria × ananassa cv Paros and identified as Serratia rubidaea strain Mar61-01 based on phenotypic, biochemical characteristics and molecular phylogenetic analysis. Antagonistic activit...

This research investigated the impact of calcium carbonate precipitation induced by the activity of four microbial strains known as Sporosarcina pasteurii, Bacillus megaterium, Sporosarcina ureae, and Bacillus licheniformis at cell concentrations of 107 cells/ml on lightweight fiber concrete (LWFC). Three mixed designs with different fiber percenta...

An endophytic bacterial strain, isolated from Fragaria × ananassa roots, and its antifungal activity against Colletotrichum nymphaeae were investigated under in vitro, in vivo, and greenhouse experiments. Bacterial isolate was identified as Bacillus atrophaeus strain DM6120 using phenotypic, biochemical, and molecular phylogenetic analysis of the 1...

The purpose of this research was to explore the control of common bean root rot disease caused by Fusarium solani by the bacterial strain CTA8689 under in vitro and greenhouse conditions. The strain CTA8689 was isolated from common bean root and identified as Achromobacter xylosoxidans using 16S rDNA sequence, morphological and physiological charac...

In this study, four endophytic bacterial strains were isolated from healthy strawberry plant and were identified as Bacillus spp. strain YN8, Bacillus spp. strain LN57, Bacillus spp. strain MN17 and Pseudomonas aeruginosa strain EN18 based on phenotypic, biochemical characteristics and molecular phylogenetic analysis. Then, their effects as biocont...

Introduction: Copper nanoparticles (CuNPs) are used in various industries, including pharmaceutical and biomedical sciences, agricultural and food chemistry, electronics and environmental applications, due to their novel optical, chemical, photoelectrochemical, and electronic properties as well as antimicrobial activities. The aim of the present st...

Copper nanoparticles (CuNPs) are used in various industries, including pharmaceutical and biomedical sciences, agricultural and food chemistry, electronics and environmental applications, due to their novel optical, chemical, photoelectrochemical and electronic properties as well as antimicrobial activities. The aim of this study was to evaluate th...

Ultrasound-assisted Fenton-like was utilized to leaching precious metals from spent automobile catalysts. The effect of different factors including solid to liquid (S/L) ratio, H2O2 percentage and initial Fe2+ concentration was done. Response surface methodology (RSM) was used to optimize the effective factors in the leaching of Al and Ce from the...

In this study, the extraction of rare metals from the spent automobile catalyst using Aspergillus niger (A. niger) was investigated. The effect of different factors including initial glucose, initial pH, inoculum percentage, and Solid to Liquid (S/L) ratio on the A.niger growth was done. Then in the condition of initial glucose of 100 g/L, initial...

Proteins have evolved in specific 3D structures and play different functions in cells and determine various reactions and pathways. The newly synthesized amino acid chains once depart ribosome must crumple into three-dimensional structures so can be biologically active. This process of protein that makes a functional molecule is called protein fold...

The aim of this study was to isolate and characterize marine bacterial strains capable of converting selenite to elemental selenium with the formation of Se nanoparticles (SeNPs). For the first time, a novel marine strain belonging to Bacillus amyloliquefaciens (GenBank accession no. MK392020) was isolated from the coast of the Caspian Sea and char...

A major challenge for functional foods containing probiotics is to provide a sufficient number of probiotic microorganisms to survive the harsh environments during processing and storage so that they can confer their health benefits. Extensive studies have been carried out on the spray-drying of probiotics based on enhancing their survivability aft...

This study was conducted to evaluate the effects of fiber source (wheat bran (WB) or sugar beet pulp (SBP)) and xylanase supplementation on production, egg quality, ileal digestibility, intestinal morphology, and gastrointestinal pH in aged laying hens. A total of 540 laying hens (Lohman LSL Lite; 70 wk of lay) were randomized into 10 treatments (6...

A major challenge for functional foods containing probiotics is to provide a sufficient number of probiotic microorganisms to survive the harsh environments during processing and storage so that they can confer their health benefits. Extensive studies have been carried out on the spray-drying of probiotics based on enhancing their survivability aft...

A novel endophytic bacterium, designated strain UN1512, was isolated from strawberry leaves, and its antifungal activity against Colletotrichum nymphaeae was estimated under in vitro, in vivo, and greenhouse conditions. The bacterial isolate was identified as Stenotrophomonas maltophilia via morphological, biochemical, and molecular traits. The str...

Crown gall disease of grapevine, caused by Agrobacterium tumefaciens, often results in severe economic loss to grape production worldwide. This study demonstrated the ability of the endophytic bacteria Pseudomonas sp. Sn48 and Pantoea sp. Sa14 isolated from domesticated and wild grapevines to induce resistance in both above‐ and belowground tissues...

An environment-friendly, cheap method, biogenic synthesis of silver nanoparticles (AgNPs) is interesting as compared to physical and chemical synthesis methods. The aim of the present study was to utilize the inherent capability of Yarrowia lipolytica as a novel biocatalyst for green production of AgNPs using different strategies, including growing...

نانوذرات سولفید کادمیوم به دلیل ویژگی های خاص فوتوالکتریکی کاربردهای فراوانی در ساخت سلولهای خورشیدی حساس به رنگ، دیودهای انتشارگر نوری و ساخت نانوبیوسنسورها دارند. با توجه به سازگار نبودن روش‌هاي متنوع فيزيکي و شيميايي با محيط زيست، توسعه روش‌های زیستی تولید نانوذرات، به‌عنوان روشی سازگار با محیط زیست و ارزان، مطرح است. علیرغم سمیت کادمیوم برای بس...

For the first time a novel aquatic bacterium belonging to strain of Pseudomonas chlororaphis was developed for the synthesis of cadmium sulfide quantum dots. The cadmium sulfate solution incubated with cell-free extract (CFE) of P. chlororaphis strain CHR05 to generate cadmium sulfide nanoparticles (CdSNps) which were characterized with combined sp...

A nonpathogenic endophytic bacterial isolate, recovered from Fragaria × ananassa stolon, and its antifungal activity against Colletotrichum nymphaeae was evaluated under in vitro, in vivo, and greenhouse conditions. Bacterial isolate was identified as Staphylococcus sciuri MarR44 (Strain ID: WDCM 891 = CCSM-B 00640) using phenotypic and biochemical...

Take-all caused by Gaeumannomyces graminis var. tritici (Ggt) is one of the most important root and crown diseases of wheat. In this research, four basidiomycetes isolates as fungal endophytes were isolated from healthy cereal plants and were identified as Coprinopsis urticicola M2, Rhizoctonia endophytica M9, Rhizoctonia zeae M32 and Fomes fomenta...

Nanotechnology is an interdisciplinary science that involves the production, manipulation, and use of materials with nano-scale. Nanobiotechnology has emerged as an important branch of nanotechnology. The combination of biological principles with physical and chemical processes generates specific nano-sized structures. Nanobiotechnology applies the...

L-DOPA (L-3, 4-dihydroxyphenylalanine) is the most effective treatment for Parkinson's disease. In the present research, isolation and characterization of L-tyrosine-degrading yeast strains and evaluation of their ability as biocatalyst for the biological conversion of L-tyrosine into L-dopa were investigated. L-tyrosine-degrading yeast strain Mar1...

Four coordination complexes, [2-mimH]2[Co(pydc)2][ClO4]. H2O (1), [2-mimH]2[Zr(pydc)3] (2), [imiH]2[Zr(pydc)3].4H2O (3) and [Ca(pydcH2)2(H2O)]2[Co(pydc)2]2·6H2O (4) have been prepared from the reaction between proton-transfer compounds containing pyridine-2,6-dicarboxylic acid (pydcH2) with 2-methylimidazole (2-mim) and imidazole (imi) in 1:2 M rat...

40 isoeugenol-tolerant yeasts were isolated from the rhizosphere soil samples which in turn were collected from aromatic plants in different regions of Iran, and further tested for their ability to grow on a minimal medium containing isoeugenol as the sole carbon and energy source. Nine isolates which were able to grow on isoeugenol were examined f...

A total of 40 fungal cultures were isolated for their ability to grow on caffeine as a sole source carbon and nitrogen, and further screened for theophylline-producing activities under the growing cell system. Based on thin-layer chromatography and high-performance liquid chromatography analyses, the potent strain Aureobasidium sp. TeO12 was chosen...

زمینه و هدف: حضور تراکم های بالای سلنیت در پساب‌های صنعتی و ورود آن به منابع آب و زنجیره غذایی موجب نگرانی های بهداشتی می شود. بنابراین حذف این آلاینده‌ سمی با روش‌های امن هم چون اصلاح زیستی میکروبی امری ضروری می‌باشد. هدف از این مطالعه، بررسی توانایی حذف سلنیت در لاکتوباسیل های مقاوم جدا شده از پنیرهای سنتی مناطق بکر روستایی استان کردستان است. روش...

Background & Aims: High concentrations of selenite have carcinogenetic, cytotoxic and genotoxic effects. Therefore, removal of this toxic pullutant from the environment has a particular importance in maintaining public health. In this study, with the aim of optimization of selenite removal process, the design Box-Behnken method was used to estimate...

In this study, about 112 isolates of Streptomyces were isolated from chickpea rhizospheric soils. Among the isolated strains, five showed strong inhibitory effects against chickpea Fusarium wilt caused by Fusarium oxysporum f. sp. ciceris in vitro using plate assay and selected for further studies. The selected strains were identified as Streptomyc...

Sesquiterpene lactones (SLs) are one of the most diverse groups of secondary metabolites that mainly have been observed in the Asteraceae. They are composed of a C15 skeleton bearing functional groups, e.g., hydroxy, keto, or epoxy. Sesquiterpene lactones have been shown to display several biological activities; hence, their therapeutic effects are...

Microbial caffeine removal is a green solution for treatment of caffeinated products and agro-industrial effluents. We directed this investigation to optimizing a bio-decaffeination process with growing cultures of Pseudomonas pseudoalcaligenes through Taguchi methodology which is a structured statistical approach that can be lowered variations in...

Background and Objectives: Microbial caffeine removal is a green solution for treatment of caffeinated products and agro-industrial effluents. We directed this investigation to optimizing a bio-decaffeination process with growing cultures of Pseudomonas pseudoalcaligenes through Taguchi methodology which is a structured statistical approach that ca...

Introduction: The biological synthesis of nanoparticles has gained enormous importance due to the development of clean and environmentally-friendly processes. Silver is highly toxic to microbial cells, Nevertheless, it has been reported that several microorganisms are silver resistance and corroborate the microbial reduction of water soluble Ag+ to...

Development of an environmental friendly and cost-effective process for microbial degradation of caffeine to non-toxic compounds are promising to solve the problems of physiochemical extraction of caffeine in the treatment the caffeine containing agroindustrial effluents. Thirteen bacterial strains, isolated from tea plantation soils in the north r...

For all industrial processes, modelling, optimisation and control are the keys to enhance productivity and ensure product quality. In the current study, the optimization of process parameters for improving the conversion of isoeugenol to vanillin by Psychrobacter sp. CSW4 was investigated by means of Taguchi approach and Box-Behnken statistical des...

The great demand of people for consumption of natural additives resulted in producing natural vanillin. There are plant sources and chemical procedures for vanillin production but microbial bioconversions are being sought as a suitable alternative. In the present work, the ability to produce vanillin from isoeugenol was screened using growing cultu...

Candida galli strain PGO6 isolated from oil-contaminated water is the first isolated yeast strain which is capable to form vanillin and vanillic acid during isoeugenol biotransformation. The products were confirmed by thin-layer chromatography (TLC), changes in the UV absorption pattern and high-performance liquid chromatography (HPLC). The phenoty...

A novel moderately halophilic, Gram-positive bacterium, designated strain QW6(T), was isolated from textile industry wastewater in Qom, Iran. Strain QW6(T) was strictly aerobic, non-motile, non-sporulating and oxidase- and catalase-positive. It grew at salinities of 1-25% (w/v) NaCl, showing optimal growth at 7.5-10.0% (w/v). Growth occurred at 5.0...

Among the 49 strains of moderately halophilic bacteria isolated from the salty environments of Iran, a Gram-positive coccus designated as strain QW6 showed high capacity in the removal of toxic oxyanions of tellurium in a wide range of culture medium factors including pH (5.5-10.5), temperature (25-45 degrees C), various salts including NaCl, KCl,...

Halophilic microorganisms have important roles in bioremediation and transformation of the toxic metal compounds in polluted environments. Identification of toxic metal tolerant strains is the first step in applying them in bioremediation processes. In these investigations 90 strains of bacteria were isolated from various salty environments (soil,...