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Monique F van Wordragen

Monique F van Wordragen
AIRINOV Nederland BV

PhD

About

62
Publications
2,370
Reads
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1,104
Citations
Introduction
Skills and Expertise
Additional affiliations
September 2006 - March 2013
NSure Holding
Position
  • CEO
September 1996 - September 2006
Wageningen University & Research
Position
  • Program manager Molecular technologies
July 1991 - September 1996
Wageningen University & Research
Position
  • PostDoc Position

Publications

Publications (62)
Article
During wintertime Viburnum opulus var. roseum (snowball) shrubs are forced in warm greenhouses to harvest early cut flowers. Early forcing is occasionally unsuccessful. This is probably due to a lack of hours with low temperatures that is needed to break bud dormancy. To gain more insight about the effect of temperature on breaking dormancy and ear...
Article
Full-text available
In boreal forest regions, a great portion of forest tree seedlings are stored indoors in late autumn to prevent seedlings from outdoor winter damage. For seedlings to be able to survive in storage it is crucial that they store well and can cope with the dark and cold storage environment. The aim of this study was to search for genes that can determ...
Patent
The invention relates to the field of quality testing of fresh plant-based and mushroom based products. Methods, carriers and kits for determining the quality stage are provided.
Article
The invention relates to the field of quality testing of fresh potato products. Methods, carriers and kits for determining and/or predicting the quality stage of potato batches are provided.
Article
Full-text available
cDNA microarrays were used to characterize senescence-associated gene expression in petals of cut carnation (Dianthus caryophyllus) flowers, sampled from anthesis to the first senescence symptoms. The population of PCR fragments spotted on these microarrays was enriched for flower-specific and senescence-specific genes, using subtractive hybridizat...
Article
Full-text available
Scots pine (Pinus sylvestris L.) seedlings were grown under different conditions (three field locations, two seasons and two climate room regimes), and then analyzed for freezing tolerance of shoots and roots and for transcript abundance in apical buds based on a cDNA microarray containing about 1500 expressed sequence tags (ESTs) from buds of cold...
Article
The current invention provides a method for rapid testing of stress tolerance in a plant and a method of producing plants with enhanced stress tolerance, in particular cold and/or drought tolerance. Such a method may be applied in breeding and selection programs for this trait, or for determining the timing of induction of stress and cold tolerance...
Article
We investigated changes in gene expression in Iris hollandicaflowers by microarray technology. Flag tepals were sampled daily, from three days prior to flower opening to the onset of visible senescence symptoms. Gene expression profiles were compared with biochemical data including lipid and protein degradation and DNA coiling, and with morphologic...
Article
Full-text available
Genomics technologies can be applied for unravelling the biological processes that are important for pre-and post-harvest quality. Such knowledge will allow precise monitoring of physiological condition and can be used as a tool for optimising production chains. A combination with quality change models will allow prediction of future product qualit...
Article
Genomics technologies can be applied for unravelling the biological processes that are important for pre- and post-harvest quality. Such knowledge will allow precise monitoring of physiological condition and can be used as a tool for optimising production chains. A combination with quality change models will allow prediction of future product quali...
Article
Full-text available
Gene expression profiles of cut Iris and carnation flowers were studied using cDNA microarrays. The cDNA libraries were enriched for flower-specific genes by subtraction with cDNA from subtending growing tissue. This strategy is meant to eliminate most household genes and numerous genes that are not specific for petals and senescence. In Iris, we s...
Chapter
Genetic manipulation has proven an extremely powerful tool in both fundamental and applied plant research. A wide variety of techniques — ranging from microinjection to particle bombardment — has been developed to allow transformation of virtually any plant species of interest. However, one thing that these techniques have in common is that, until...
Article
This article describes a set of protocols—for retrofitting, transformation and purification—that together enable the delivery of full-sized YAC-DNA to plant cells. To be able to equip YACs of interest with plant selectable markers, we have constructed a retrofitting vector that carriesnptII anduidA. Furthermore, we established a transformation prot...
Article
Variation in recombinant frequencies at two adjacent intervals on chromosome 6 of tomato (Lycopersicon esculentum Mill.) has been studied in seven lines that differ in the amount and origin of introgressed segments from wild species. These lines were all crossed to a genotype homozygous recessive for the markers tl, yv, and c, which define the cent...
Article
Integration of molecular and classical genetic maps is an essential requirement for marker-assisted breeding, quantitative trait locus mapping and map-based cloning. With respects to tomato, such maps are only available for the top part of chromosome 1, for chromosome 3 and for the short arm and the centromere proximal part of the long arm of chrom...
Article
Full-text available
In the past, a classical map of the tomato genome has been established that is based on linkage data from intraspecific Lycopersicon esculentum crosses. In addition, a high density molecular linkage map has recently been constructed using a L. esculentum x L. pennellii cross. As the respective maps only partially match, they provide limited informa...
Article
We have previously presented an integrated linkage map of tomato chromosome 6, that showed the position of restriction fragment length polymorphism (RFLP) and randomly amplified polymorphic DNA (RAPD) markers relative to a variety of classical markers. As for the short arm, map resolution has now been improved by crossing the chromosome 6 substitut...
Article
A 3'-end truncated crystal protein gene, derived from Bacillus thuringiensis (Bt) subsp. aizawai 7.21, encoding the toxic fragment of the insecticidal protein cryIA(b), was constructed. The gene was inserted into a transformation vector, also carrying the neomycin phosphotransferase II (nptII) gene and the beta-glucuronidase (gus) gene, and introdu...
Article
Explants from leaves of in vitro-grown chrysanthemum (Dendranthema grandiflora Tzvel.) cultivars regenerated adventitious shoots without an intermediate callus phase. Puncturing explants with a brush increased regenerations, but in combination with cocultivation with Agrobacterium tumefaciens it had an adverse effect on shoot formation. The negativ...
Article
The susceptibilities to gene transfer of seven genotypes of chrysanthemum (Dendranthema grandiflora), upon infection of leaf explants with wild-type Agrobacterium strains, were compared in a search for highly efficient cultivar/bacterial strain combinations. Large differences between genotypes were found, but for all genotypes the supervirulent str...
Article
Full-text available
A dominant allele at the Mi locus on chromosome 6 of tomato (Lycopersicon esculentum Mill) confers resistance to three species of root-knot nematodes (Meloidogyne). The resistance, which is associated with a localized necrotic response, was originally introduced into tomato from the wild species Lycopersicon peruvianum. As a step towards the molecu...
Article
A dominant allele at the Mi locus on chromosome 6 of tomato (Lycopersicon esculentum Mill) confers resistance to three species of root-knot nematodes (Meloidogyne). The resistance, which is associated with a localized necrotic response, was originally introduced into tomato from the wild species Lycopersicon peruvianum. As a step towards the molecu...
Article
The most widely used technique for the introduction of new genetic information into plant cells is based on the natural gene transfer capacity ofAgrobacterium tumefaciens. Currently, this technique is routinely applicable in just a few model species, like tobacco and petunia. Thus far, the numerous efforts to apply the technique to crop species hav...
Article
The agropine type Agrobacterium rhizogenes strain LBA9402 induced callus and roots on stems of greenhouse grown plants and on leaf disks of in vitro grown plantlets of chrysanthemum (Dendranthema grandiflora Tzvel.). In this callus and roots no opines were detected, nor were any of the other features of the hairy root syndrome observed. Experiments...
Article
To develop an Agrobacterium mediated transformation protocol for chrysanthemum we studied the transformation efficiency of commonly used A.tumefaciens strains on 14 genotypes by comparing tumour size and frequency. One genotype was analyzed in detail using 14 strains of both A.tumefaciens and A.rhizogenes. Only a few genotype/strain combinations re...
Article
Thesis statement, summary, afterword, and vita in Dutch. "Stellingen" ([2] p.) inserted. Thesis (doctoral)--Landbouwuniversiteit te Wageningen, 1991. Includes bibliographical references.
Article
Microarrays were used to identify genes involved in dormancy and frost tolerance in tree seedlings. For Pinus sylvestris, expression profiles of more than 1500 ESTs were determined during autumn and winter. Seedlings were grown at locations in Denmark, Scotland and Sweden and several provenances were used including seedlings of varying age. From th...
Article
We investigated changes in gene expression in Iris hollandicaflowers by microarray technology. Flag tepals were sampled daily, from three days prior to flower opening to the onset of visible senescence symptoms. Gene expression profiles were compared with biochemical data including lipid and protein degradation and DNA coiling, and with morphologic...

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